Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale

Autores
Giorello, Alejandra Nahili; Kennedy, Malcolm W.; Butti, Marcos Javier; Radman, Nilda Ester; Córsico, Betina; Franchini, Gisela Raquel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The giant kidney worm, Dioctophyme renale, is a debilitating and potentially lethal parasite that inhabits and destroys, typically host´s right kidney, and may also be found in ectopic sites. It is circumglobally distributed, mainly in dogs, and is increasingly regarded as a threat to other domestic animals and humans. There is little information on the parasite´s true incidence, or immune responses to it, and none on its biochemistry and molecular biology. Results: We characterised the soluble proteins of body wall, intestine, gonads and pseudocelomic fluid (PCF) of adult parasites. Two proteins, P17 and P44, dominate the PCF of both male and females. P17 is of 16,622 Da by mass spectrometry, and accounts for the intense red colour of the adult parasites. It may function to carry or scavenge oxygen and be related to the ´nemoglobins´ found in other nematode clades. P44 is of 44,460 Da and was found to associate with fatty acids by thin layer chromatography. Using environment-sensitive fluorescent lipid probes, P44 proved to be a hydrophobic ligand-binding protein with a binding site that is highly apolar, and competitive displacement experiments showed that P44 binds fatty acids. It may therefore have a role in distributing lipids within the parasites and, if also secreted, might influence local inflammatory and tissue responses. N-terminal and internal peptide amino-acid sequences of P44 indicate a relationship with a cysteine- and histidine-rich protein of unknown function from Trichinella spiralis. Conclusions: The dominant proteins of D. renale PCF are, like those of large ascaridids, likely to be involved in lipid and oxygen handling, although there is evidence of strong divergence between the two groups.
Fil: Giorello, Alejandra Nahili. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Kennedy, Malcolm W.. University of Glasgow; Reino Unido
Fil: Butti, Marcos Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Radman, Nilda Ester. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Córsico, Betina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Franchini, Gisela Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Materia
DIOCTOPHYME RENALE
EMERGING ZOONOSES
LIPID-BINDING PROTEINS
NEMATODES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48543
Acceder
id CONICETDig_bc80a5bd615b1cdfbfaff151446c632e
oai_identifier_str oai:ri.conicet.gov.ar:11336/48543
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renaleGiorello, Alejandra NahiliKennedy, Malcolm W.Butti, Marcos JavierRadman, Nilda EsterCórsico, BetinaFranchini, Gisela RaquelDIOCTOPHYME RENALEEMERGING ZOONOSESLIPID-BINDING PROTEINSNEMATODEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: The giant kidney worm, Dioctophyme renale, is a debilitating and potentially lethal parasite that inhabits and destroys, typically host´s right kidney, and may also be found in ectopic sites. It is circumglobally distributed, mainly in dogs, and is increasingly regarded as a threat to other domestic animals and humans. There is little information on the parasite´s true incidence, or immune responses to it, and none on its biochemistry and molecular biology. Results: We characterised the soluble proteins of body wall, intestine, gonads and pseudocelomic fluid (PCF) of adult parasites. Two proteins, P17 and P44, dominate the PCF of both male and females. P17 is of 16,622 Da by mass spectrometry, and accounts for the intense red colour of the adult parasites. It may function to carry or scavenge oxygen and be related to the ´nemoglobins´ found in other nematode clades. P44 is of 44,460 Da and was found to associate with fatty acids by thin layer chromatography. Using environment-sensitive fluorescent lipid probes, P44 proved to be a hydrophobic ligand-binding protein with a binding site that is highly apolar, and competitive displacement experiments showed that P44 binds fatty acids. It may therefore have a role in distributing lipids within the parasites and, if also secreted, might influence local inflammatory and tissue responses. N-terminal and internal peptide amino-acid sequences of P44 indicate a relationship with a cysteine- and histidine-rich protein of unknown function from Trichinella spiralis. Conclusions: The dominant proteins of D. renale PCF are, like those of large ascaridids, likely to be involved in lipid and oxygen handling, although there is evidence of strong divergence between the two groups.Fil: Giorello, Alejandra Nahili. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Kennedy, Malcolm W.. University of Glasgow; Reino UnidoFil: Butti, Marcos Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Radman, Nilda Ester. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Córsico, Betina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Franchini, Gisela Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaBioMed Central2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48543Giorello, Alejandra Nahili; Kennedy, Malcolm W.; Butti, Marcos Javier; Radman, Nilda Ester; Córsico, Betina; et al.; Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale; BioMed Central; Parasites and Vectors; 10; 1; 9-2017; 1-111756-3305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-017-2388-xinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-017-2388-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:39:06Zoai:ri.conicet.gov.ar:11336/48543instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:39:07.243CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale
title Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale
spellingShingle Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale
Giorello, Alejandra Nahili
DIOCTOPHYME RENALE
EMERGING ZOONOSES
LIPID-BINDING PROTEINS
NEMATODES
title_short Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale
title_full Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale
title_fullStr Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale
title_full_unstemmed Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale
title_sort Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale
dc.creator.none.fl_str_mv Giorello, Alejandra Nahili
Kennedy, Malcolm W.
Butti, Marcos Javier
Radman, Nilda Ester
Córsico, Betina
Franchini, Gisela Raquel
author Giorello, Alejandra Nahili
author_facet Giorello, Alejandra Nahili
Kennedy, Malcolm W.
Butti, Marcos Javier
Radman, Nilda Ester
Córsico, Betina
Franchini, Gisela Raquel
author_role author
author2 Kennedy, Malcolm W.
Butti, Marcos Javier
Radman, Nilda Ester
Córsico, Betina
Franchini, Gisela Raquel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DIOCTOPHYME RENALE
EMERGING ZOONOSES
LIPID-BINDING PROTEINS
NEMATODES
topic DIOCTOPHYME RENALE
EMERGING ZOONOSES
LIPID-BINDING PROTEINS
NEMATODES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: The giant kidney worm, Dioctophyme renale, is a debilitating and potentially lethal parasite that inhabits and destroys, typically host´s right kidney, and may also be found in ectopic sites. It is circumglobally distributed, mainly in dogs, and is increasingly regarded as a threat to other domestic animals and humans. There is little information on the parasite´s true incidence, or immune responses to it, and none on its biochemistry and molecular biology. Results: We characterised the soluble proteins of body wall, intestine, gonads and pseudocelomic fluid (PCF) of adult parasites. Two proteins, P17 and P44, dominate the PCF of both male and females. P17 is of 16,622 Da by mass spectrometry, and accounts for the intense red colour of the adult parasites. It may function to carry or scavenge oxygen and be related to the ´nemoglobins´ found in other nematode clades. P44 is of 44,460 Da and was found to associate with fatty acids by thin layer chromatography. Using environment-sensitive fluorescent lipid probes, P44 proved to be a hydrophobic ligand-binding protein with a binding site that is highly apolar, and competitive displacement experiments showed that P44 binds fatty acids. It may therefore have a role in distributing lipids within the parasites and, if also secreted, might influence local inflammatory and tissue responses. N-terminal and internal peptide amino-acid sequences of P44 indicate a relationship with a cysteine- and histidine-rich protein of unknown function from Trichinella spiralis. Conclusions: The dominant proteins of D. renale PCF are, like those of large ascaridids, likely to be involved in lipid and oxygen handling, although there is evidence of strong divergence between the two groups.
Fil: Giorello, Alejandra Nahili. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Kennedy, Malcolm W.. University of Glasgow; Reino Unido
Fil: Butti, Marcos Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Radman, Nilda Ester. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Córsico, Betina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Franchini, Gisela Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
description Background: The giant kidney worm, Dioctophyme renale, is a debilitating and potentially lethal parasite that inhabits and destroys, typically host´s right kidney, and may also be found in ectopic sites. It is circumglobally distributed, mainly in dogs, and is increasingly regarded as a threat to other domestic animals and humans. There is little information on the parasite´s true incidence, or immune responses to it, and none on its biochemistry and molecular biology. Results: We characterised the soluble proteins of body wall, intestine, gonads and pseudocelomic fluid (PCF) of adult parasites. Two proteins, P17 and P44, dominate the PCF of both male and females. P17 is of 16,622 Da by mass spectrometry, and accounts for the intense red colour of the adult parasites. It may function to carry or scavenge oxygen and be related to the ´nemoglobins´ found in other nematode clades. P44 is of 44,460 Da and was found to associate with fatty acids by thin layer chromatography. Using environment-sensitive fluorescent lipid probes, P44 proved to be a hydrophobic ligand-binding protein with a binding site that is highly apolar, and competitive displacement experiments showed that P44 binds fatty acids. It may therefore have a role in distributing lipids within the parasites and, if also secreted, might influence local inflammatory and tissue responses. N-terminal and internal peptide amino-acid sequences of P44 indicate a relationship with a cysteine- and histidine-rich protein of unknown function from Trichinella spiralis. Conclusions: The dominant proteins of D. renale PCF are, like those of large ascaridids, likely to be involved in lipid and oxygen handling, although there is evidence of strong divergence between the two groups.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48543
Giorello, Alejandra Nahili; Kennedy, Malcolm W.; Butti, Marcos Javier; Radman, Nilda Ester; Córsico, Betina; et al.; Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale; BioMed Central; Parasites and Vectors; 10; 1; 9-2017; 1-11
1756-3305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48543
identifier_str_mv Giorello, Alejandra Nahili; Kennedy, Malcolm W.; Butti, Marcos Javier; Radman, Nilda Ester; Córsico, Betina; et al.; Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale; BioMed Central; Parasites and Vectors; 10; 1; 9-2017; 1-11
1756-3305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-017-2388-x
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-017-2388-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083508260306944
score 13.22299

Publicaciones similares