Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7
- Autores
- Goñi, María Gabriela; Tomadoni, Bárbara María; Roura, Sara Ines; Moreira, Maria del Rosario
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The main objective was to assess the effectiveness of preharvest application of chitosan (CH) and tea tree essential oil (TT) in butterhead lettuce in controlling native microflora growth and counteract exogenous Escherichia coli O157:H7 contamination. TT and CH were applied to lettuce plants at 14, 10, 7, 3 and 0 days before harvest. CH showed significant reductions at harvest on mesophilic (−2.0 log cfu/g), psychrotrophic (−1.0 log cfu/g) and yeast and molds (−1.8 log cfu/g), compared with control samples. CH also reduced total coliform bacteria (−2.0 log cfu/g) compared with inoculated plants. Furthermore, CH exerted a bacteriostatic effect on E. coli. TT was only able to reduce total coliforms compared with inoculated plants. Preharvest application of CH exerted an inhibitory effect on lettuce native microflora and a bacteriostatic effect on exogenous E. coli during postharvest storage. Therefore, CH appears to be a promising method for enhancing the safety of lettuce, exposed to an inadequate postharvest handling. Practical Applications Consumers demand products with less use of chemicals; therefore, there has been an increasing research for natural antimicrobials. Chitosan (CH) and essential oils, such as tea tree (TT), have become promising alternatives to chemical decontamination because of its natural character, antimicrobial activity and elicitation of defense responses in plant tissue. The objectives of this work were to examine the residual effectiveness of CH and TT solutions, applied during preharvest, to control E. coli O157:H7 inoculated in lettuce heads, simulating an inadequate manipulation of the vegetable at postharvest. CH solution was a good alternative for controlling not only the native microflora in lettuce during storage, but also reducing the survival of E. coli inoculated in the vegetable at harvest. Preharvest application of CH in the late stages of lettuce head development could easily be introduce in the good agricultural practice routine, without consequences on the environment because CH is a generally recognized as safe substance.
Fil: Goñi, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina
Fil: Tomadoni, Bárbara María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina
Fil: Roura, Sara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina
Fil: Moreira, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina - Materia
-
Biopreservatives
Pathogen Control
Microbial Evolution
Leafy Vegetables - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/35349
Ver los metadatos del registro completo
id |
CONICETDig_bc7a84b33f5f10e35dec566da1c85422 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/35349 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7Goñi, María GabrielaTomadoni, Bárbara MaríaRoura, Sara InesMoreira, Maria del RosarioBiopreservativesPathogen ControlMicrobial EvolutionLeafy Vegetableshttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The main objective was to assess the effectiveness of preharvest application of chitosan (CH) and tea tree essential oil (TT) in butterhead lettuce in controlling native microflora growth and counteract exogenous Escherichia coli O157:H7 contamination. TT and CH were applied to lettuce plants at 14, 10, 7, 3 and 0 days before harvest. CH showed significant reductions at harvest on mesophilic (−2.0 log cfu/g), psychrotrophic (−1.0 log cfu/g) and yeast and molds (−1.8 log cfu/g), compared with control samples. CH also reduced total coliform bacteria (−2.0 log cfu/g) compared with inoculated plants. Furthermore, CH exerted a bacteriostatic effect on E. coli. TT was only able to reduce total coliforms compared with inoculated plants. Preharvest application of CH exerted an inhibitory effect on lettuce native microflora and a bacteriostatic effect on exogenous E. coli during postharvest storage. Therefore, CH appears to be a promising method for enhancing the safety of lettuce, exposed to an inadequate postharvest handling. Practical Applications Consumers demand products with less use of chemicals; therefore, there has been an increasing research for natural antimicrobials. Chitosan (CH) and essential oils, such as tea tree (TT), have become promising alternatives to chemical decontamination because of its natural character, antimicrobial activity and elicitation of defense responses in plant tissue. The objectives of this work were to examine the residual effectiveness of CH and TT solutions, applied during preharvest, to control E. coli O157:H7 inoculated in lettuce heads, simulating an inadequate manipulation of the vegetable at postharvest. CH solution was a good alternative for controlling not only the native microflora in lettuce during storage, but also reducing the survival of E. coli inoculated in the vegetable at harvest. Preharvest application of CH in the late stages of lettuce head development could easily be introduce in the good agricultural practice routine, without consequences on the environment because CH is a generally recognized as safe substance.Fil: Goñi, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Tomadoni, Bárbara María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Roura, Sara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Moreira, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaWiley Blackwell Publishing, Inc2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35349Goñi, María Gabriela; Tomadoni, Bárbara María; Roura, Sara Ines; Moreira, Maria del Rosario; Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7; Wiley Blackwell Publishing, Inc; Journal Of Food Safety; 34; 4; 7-2014; 353-3600149-6085CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/jfs.12135info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jfs.12135/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:05Zoai:ri.conicet.gov.ar:11336/35349instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:05.867CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7 |
title |
Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7 |
spellingShingle |
Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7 Goñi, María Gabriela Biopreservatives Pathogen Control Microbial Evolution Leafy Vegetables |
title_short |
Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7 |
title_full |
Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7 |
title_fullStr |
Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7 |
title_full_unstemmed |
Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7 |
title_sort |
Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7 |
dc.creator.none.fl_str_mv |
Goñi, María Gabriela Tomadoni, Bárbara María Roura, Sara Ines Moreira, Maria del Rosario |
author |
Goñi, María Gabriela |
author_facet |
Goñi, María Gabriela Tomadoni, Bárbara María Roura, Sara Ines Moreira, Maria del Rosario |
author_role |
author |
author2 |
Tomadoni, Bárbara María Roura, Sara Ines Moreira, Maria del Rosario |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Biopreservatives Pathogen Control Microbial Evolution Leafy Vegetables |
topic |
Biopreservatives Pathogen Control Microbial Evolution Leafy Vegetables |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
The main objective was to assess the effectiveness of preharvest application of chitosan (CH) and tea tree essential oil (TT) in butterhead lettuce in controlling native microflora growth and counteract exogenous Escherichia coli O157:H7 contamination. TT and CH were applied to lettuce plants at 14, 10, 7, 3 and 0 days before harvest. CH showed significant reductions at harvest on mesophilic (−2.0 log cfu/g), psychrotrophic (−1.0 log cfu/g) and yeast and molds (−1.8 log cfu/g), compared with control samples. CH also reduced total coliform bacteria (−2.0 log cfu/g) compared with inoculated plants. Furthermore, CH exerted a bacteriostatic effect on E. coli. TT was only able to reduce total coliforms compared with inoculated plants. Preharvest application of CH exerted an inhibitory effect on lettuce native microflora and a bacteriostatic effect on exogenous E. coli during postharvest storage. Therefore, CH appears to be a promising method for enhancing the safety of lettuce, exposed to an inadequate postharvest handling. Practical Applications Consumers demand products with less use of chemicals; therefore, there has been an increasing research for natural antimicrobials. Chitosan (CH) and essential oils, such as tea tree (TT), have become promising alternatives to chemical decontamination because of its natural character, antimicrobial activity and elicitation of defense responses in plant tissue. The objectives of this work were to examine the residual effectiveness of CH and TT solutions, applied during preharvest, to control E. coli O157:H7 inoculated in lettuce heads, simulating an inadequate manipulation of the vegetable at postharvest. CH solution was a good alternative for controlling not only the native microflora in lettuce during storage, but also reducing the survival of E. coli inoculated in the vegetable at harvest. Preharvest application of CH in the late stages of lettuce head development could easily be introduce in the good agricultural practice routine, without consequences on the environment because CH is a generally recognized as safe substance. Fil: Goñi, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina Fil: Tomadoni, Bárbara María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina Fil: Roura, Sara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina Fil: Moreira, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina |
description |
The main objective was to assess the effectiveness of preharvest application of chitosan (CH) and tea tree essential oil (TT) in butterhead lettuce in controlling native microflora growth and counteract exogenous Escherichia coli O157:H7 contamination. TT and CH were applied to lettuce plants at 14, 10, 7, 3 and 0 days before harvest. CH showed significant reductions at harvest on mesophilic (−2.0 log cfu/g), psychrotrophic (−1.0 log cfu/g) and yeast and molds (−1.8 log cfu/g), compared with control samples. CH also reduced total coliform bacteria (−2.0 log cfu/g) compared with inoculated plants. Furthermore, CH exerted a bacteriostatic effect on E. coli. TT was only able to reduce total coliforms compared with inoculated plants. Preharvest application of CH exerted an inhibitory effect on lettuce native microflora and a bacteriostatic effect on exogenous E. coli during postharvest storage. Therefore, CH appears to be a promising method for enhancing the safety of lettuce, exposed to an inadequate postharvest handling. Practical Applications Consumers demand products with less use of chemicals; therefore, there has been an increasing research for natural antimicrobials. Chitosan (CH) and essential oils, such as tea tree (TT), have become promising alternatives to chemical decontamination because of its natural character, antimicrobial activity and elicitation of defense responses in plant tissue. The objectives of this work were to examine the residual effectiveness of CH and TT solutions, applied during preharvest, to control E. coli O157:H7 inoculated in lettuce heads, simulating an inadequate manipulation of the vegetable at postharvest. CH solution was a good alternative for controlling not only the native microflora in lettuce during storage, but also reducing the survival of E. coli inoculated in the vegetable at harvest. Preharvest application of CH in the late stages of lettuce head development could easily be introduce in the good agricultural practice routine, without consequences on the environment because CH is a generally recognized as safe substance. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/35349 Goñi, María Gabriela; Tomadoni, Bárbara María; Roura, Sara Ines; Moreira, Maria del Rosario; Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7; Wiley Blackwell Publishing, Inc; Journal Of Food Safety; 34; 4; 7-2014; 353-360 0149-6085 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/35349 |
identifier_str_mv |
Goñi, María Gabriela; Tomadoni, Bárbara María; Roura, Sara Ines; Moreira, Maria del Rosario; Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7; Wiley Blackwell Publishing, Inc; Journal Of Food Safety; 34; 4; 7-2014; 353-360 0149-6085 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1111/jfs.12135 info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jfs.12135/abstract |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613268021182464 |
score |
13.070432 |