Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphisms

Autores
Lorenzetti, Mario Alejandro; Gantuz, Magdalena; Altcheh, Jaime Marcelo; de Matteo, Elena Noemí; Chabay, Paola Andrea; Preciado, María Victoria
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ubiquitous Epstein-Barr virus (EBV) is related to the development of lymphomas and is also the etiological agent for infectious mononucleosis (IM). Sequence variations in the LMP1 encoding gene have been deeply studied in different pathologies and geographic regions. Controversial results propose the existence of tumor-related variants while others argued in favor of a geographical distribution of these variants. However, current reports assessing EBV variants in IM were performed in adult patients who display multiple variant infections. In the present study, LMP1 variants in 15 pediatric patients with IM and 20 pediatric EBV-associated lymphomas from Argentina were compared as representatives of benign and malignant infection in children, respectively. A three months follow-up study of LMP1 variants in peripheral blood cells and in oral secretions of patients with IM was performed. Moreover, an integrated linkage analysis was performed with variants of EBNA1 and the promoter region of BZLF1. Similar sequence polymorphisms were detected in both pathological conditions, IM and lymphoma, but these differ from those previously described in healthy donors from Argentina and Brazil. The results suggest that certain LMP1 polymorphisms, namely the 30bp deletion and high copy number of the 33bp repeats, could be associated to EBV-related pathologies, either benign or malignant instead of just tumor related. Additionally, this is the first study to describe the Alaskan variant in EBV-related lymphomas previously restricted to nasopharyngeal carcinomas from North America..
Fil: Lorenzetti, Mario Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gantuz, Magdalena. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina
Materia
LMP1
Infectious Mononucleosis
Lymphoma
Pediatric Patients
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/244770
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/244770
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphismsLorenzetti, Mario AlejandroGantuz, MagdalenaAltcheh, Jaime Marcelode Matteo, Elena NoemíChabay, Paola AndreaPreciado, María VictoriaLMP1Infectious MononucleosisLymphomaPediatric Patientshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The ubiquitous Epstein-Barr virus (EBV) is related to the development of lymphomas and is also the etiological agent for infectious mononucleosis (IM). Sequence variations in the LMP1 encoding gene have been deeply studied in different pathologies and geographic regions. Controversial results propose the existence of tumor-related variants while others argued in favor of a geographical distribution of these variants. However, current reports assessing EBV variants in IM were performed in adult patients who display multiple variant infections. In the present study, LMP1 variants in 15 pediatric patients with IM and 20 pediatric EBV-associated lymphomas from Argentina were compared as representatives of benign and malignant infection in children, respectively. A three months follow-up study of LMP1 variants in peripheral blood cells and in oral secretions of patients with IM was performed. Moreover, an integrated linkage analysis was performed with variants of EBNA1 and the promoter region of BZLF1. Similar sequence polymorphisms were detected in both pathological conditions, IM and lymphoma, but these differ from those previously described in healthy donors from Argentina and Brazil. The results suggest that certain LMP1 polymorphisms, namely the 30bp deletion and high copy number of the 33bp repeats, could be associated to EBV-related pathologies, either benign or malignant instead of just tumor related. Additionally, this is the first study to describe the Alaskan variant in EBV-related lymphomas previously restricted to nasopharyngeal carcinomas from North America..Fil: Lorenzetti, Mario Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gantuz, Magdalena. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; ArgentinaAmerican Society for Microbiology2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244770Lorenzetti, Mario Alejandro; Gantuz, Magdalena; Altcheh, Jaime Marcelo; de Matteo, Elena Noemí; Chabay, Paola Andrea; et al.; Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphisms; American Society for Microbiology; Journal of Clinical Microbiology; 50; 3; 3-2012; 609-6180095-1137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/jcm.01043-12info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:07Zoai:ri.conicet.gov.ar:11336/244770instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:07.637CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphisms
title Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphisms
spellingShingle Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphisms
Lorenzetti, Mario Alejandro
LMP1
Infectious Mononucleosis
Lymphoma
Pediatric Patients
title_short Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphisms
title_full Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphisms
title_fullStr Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphisms
title_full_unstemmed Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphisms
title_sort Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphisms
dc.creator.none.fl_str_mv Lorenzetti, Mario Alejandro
Gantuz, Magdalena
Altcheh, Jaime Marcelo
de Matteo, Elena Noemí
Chabay, Paola Andrea
Preciado, María Victoria
author Lorenzetti, Mario Alejandro
author_facet Lorenzetti, Mario Alejandro
Gantuz, Magdalena
Altcheh, Jaime Marcelo
de Matteo, Elena Noemí
Chabay, Paola Andrea
Preciado, María Victoria
author_role author
author2 Gantuz, Magdalena
Altcheh, Jaime Marcelo
de Matteo, Elena Noemí
Chabay, Paola Andrea
Preciado, María Victoria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv LMP1
Infectious Mononucleosis
Lymphoma
Pediatric Patients
topic LMP1
Infectious Mononucleosis
Lymphoma
Pediatric Patients
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The ubiquitous Epstein-Barr virus (EBV) is related to the development of lymphomas and is also the etiological agent for infectious mononucleosis (IM). Sequence variations in the LMP1 encoding gene have been deeply studied in different pathologies and geographic regions. Controversial results propose the existence of tumor-related variants while others argued in favor of a geographical distribution of these variants. However, current reports assessing EBV variants in IM were performed in adult patients who display multiple variant infections. In the present study, LMP1 variants in 15 pediatric patients with IM and 20 pediatric EBV-associated lymphomas from Argentina were compared as representatives of benign and malignant infection in children, respectively. A three months follow-up study of LMP1 variants in peripheral blood cells and in oral secretions of patients with IM was performed. Moreover, an integrated linkage analysis was performed with variants of EBNA1 and the promoter region of BZLF1. Similar sequence polymorphisms were detected in both pathological conditions, IM and lymphoma, but these differ from those previously described in healthy donors from Argentina and Brazil. The results suggest that certain LMP1 polymorphisms, namely the 30bp deletion and high copy number of the 33bp repeats, could be associated to EBV-related pathologies, either benign or malignant instead of just tumor related. Additionally, this is the first study to describe the Alaskan variant in EBV-related lymphomas previously restricted to nasopharyngeal carcinomas from North America..
Fil: Lorenzetti, Mario Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gantuz, Magdalena. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina
description The ubiquitous Epstein-Barr virus (EBV) is related to the development of lymphomas and is also the etiological agent for infectious mononucleosis (IM). Sequence variations in the LMP1 encoding gene have been deeply studied in different pathologies and geographic regions. Controversial results propose the existence of tumor-related variants while others argued in favor of a geographical distribution of these variants. However, current reports assessing EBV variants in IM were performed in adult patients who display multiple variant infections. In the present study, LMP1 variants in 15 pediatric patients with IM and 20 pediatric EBV-associated lymphomas from Argentina were compared as representatives of benign and malignant infection in children, respectively. A three months follow-up study of LMP1 variants in peripheral blood cells and in oral secretions of patients with IM was performed. Moreover, an integrated linkage analysis was performed with variants of EBNA1 and the promoter region of BZLF1. Similar sequence polymorphisms were detected in both pathological conditions, IM and lymphoma, but these differ from those previously described in healthy donors from Argentina and Brazil. The results suggest that certain LMP1 polymorphisms, namely the 30bp deletion and high copy number of the 33bp repeats, could be associated to EBV-related pathologies, either benign or malignant instead of just tumor related. Additionally, this is the first study to describe the Alaskan variant in EBV-related lymphomas previously restricted to nasopharyngeal carcinomas from North America..
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/244770
Lorenzetti, Mario Alejandro; Gantuz, Magdalena; Altcheh, Jaime Marcelo; de Matteo, Elena Noemí; Chabay, Paola Andrea; et al.; Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphisms; American Society for Microbiology; Journal of Clinical Microbiology; 50; 3; 3-2012; 609-618
0095-1137
CONICET Digital
CONICET
url http://hdl.handle.net/11336/244770
identifier_str_mv Lorenzetti, Mario Alejandro; Gantuz, Magdalena; Altcheh, Jaime Marcelo; de Matteo, Elena Noemí; Chabay, Paola Andrea; et al.; Distinctive EBV variants associated to pediatric benign and malignant pathologies: LMP1 sequence characterization and linkage with other viral gene polymorphisms; American Society for Microbiology; Journal of Clinical Microbiology; 50; 3; 3-2012; 609-618
0095-1137
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/jcm.01043-12
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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