LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina

Autores
Gantuz, Magdalena; Lorenzetti, Mario Alejandro; Altcheh, Jaime Marcelo; de Matteo, Elena Noemí; Moscatelli, Guillermo; Moroni, Samanta; Chabay, Paola Andrea; Preciado, María Victoria
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Epstein Barr virus (EBV) is associated with several lymphoid and epithelial malignancies such as Hodgkin and Burkitt lymphoma or nasopharyngeal carcinoma and it is also the etiological agent of infectious mononucleosis (IM). Transcriptional regulation of the viral oncoprotein LMP1, remains yet not fully understood. LMP1 expression can be initiated in an EBNA2 dependent or independent manner from ED-L1 or LT-R1 promoters. It has been proposed that sequence variation at ED-L1 region could be an important factor concerning LMP1 expression. In order to characterize the natural sequence variation of the ED-L1 promoter, and its relationship with neoplasia, 44 pediatric patients, 17 IM and 27 EBV-associated lymphoma cases from Argentina, were studied. Phylogenetic analysis showed 4 main clusters, namely B95.8, Raji, Cao and P3HR1. Most isolates, 80.3%, conformed the B95.8 group. Co-infection with more than one viral variant was detected in 5/17 IM cases, but no co-infections were detected among lymphoma cases. Moreover, co-infected IM cases exhibited differences between the ED-L1 sequences obtained from different anatomical compartments. Mutations confined to transcription factor binding sites such as SP1/SP3, CRE, AP2, C/EBP were found in similar proportions in 23 isolates from both benign and malignant samples, rendering the distribution of these mutations not significant among malignant samples.
Fil: Gantuz, Magdalena. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lorenzetti, Mario Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moscatelli, Guillermo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moroni, Samanta. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina
Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Preciado, María Victoria. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Epstein Barr Virus
Lmp1
Ed-L1 Promoter
Lymphoma
Infectious Mononucleosis
Pediatric Patients
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23963
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in ArgentinaGantuz, MagdalenaLorenzetti, Mario AlejandroAltcheh, Jaime Marcelode Matteo, Elena NoemíMoscatelli, GuillermoMoroni, SamantaChabay, Paola AndreaPreciado, María VictoriaEpstein Barr VirusLmp1Ed-L1 PromoterLymphomaInfectious MononucleosisPediatric Patientshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The Epstein Barr virus (EBV) is associated with several lymphoid and epithelial malignancies such as Hodgkin and Burkitt lymphoma or nasopharyngeal carcinoma and it is also the etiological agent of infectious mononucleosis (IM). Transcriptional regulation of the viral oncoprotein LMP1, remains yet not fully understood. LMP1 expression can be initiated in an EBNA2 dependent or independent manner from ED-L1 or LT-R1 promoters. It has been proposed that sequence variation at ED-L1 region could be an important factor concerning LMP1 expression. In order to characterize the natural sequence variation of the ED-L1 promoter, and its relationship with neoplasia, 44 pediatric patients, 17 IM and 27 EBV-associated lymphoma cases from Argentina, were studied. Phylogenetic analysis showed 4 main clusters, namely B95.8, Raji, Cao and P3HR1. Most isolates, 80.3%, conformed the B95.8 group. Co-infection with more than one viral variant was detected in 5/17 IM cases, but no co-infections were detected among lymphoma cases. Moreover, co-infected IM cases exhibited differences between the ED-L1 sequences obtained from different anatomical compartments. Mutations confined to transcription factor binding sites such as SP1/SP3, CRE, AP2, C/EBP were found in similar proportions in 23 isolates from both benign and malignant samples, rendering the distribution of these mutations not significant among malignant samples.Fil: Gantuz, Magdalena. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lorenzetti, Mario Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moscatelli, Guillermo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moroni, Samanta. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; ArgentinaFil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Preciado, María Victoria. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23963Gantuz, Magdalena; Lorenzetti, Mario Alejandro; Altcheh, Jaime Marcelo; de Matteo, Elena Noemí; Moscatelli, Guillermo; et al.; LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina; Elsevier; Infection, Genetics and Evolution; 14; 1-2013; 275-2811567-1348CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567134813000105info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2012.12.025info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:49Zoai:ri.conicet.gov.ar:11336/23963instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:49.212CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina
title LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina
spellingShingle LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina
Gantuz, Magdalena
Epstein Barr Virus
Lmp1
Ed-L1 Promoter
Lymphoma
Infectious Mononucleosis
Pediatric Patients
title_short LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina
title_full LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina
title_fullStr LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina
title_full_unstemmed LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina
title_sort LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina
dc.creator.none.fl_str_mv Gantuz, Magdalena
Lorenzetti, Mario Alejandro
Altcheh, Jaime Marcelo
de Matteo, Elena Noemí
Moscatelli, Guillermo
Moroni, Samanta
Chabay, Paola Andrea
Preciado, María Victoria
author Gantuz, Magdalena
author_facet Gantuz, Magdalena
Lorenzetti, Mario Alejandro
Altcheh, Jaime Marcelo
de Matteo, Elena Noemí
Moscatelli, Guillermo
Moroni, Samanta
Chabay, Paola Andrea
Preciado, María Victoria
author_role author
author2 Lorenzetti, Mario Alejandro
Altcheh, Jaime Marcelo
de Matteo, Elena Noemí
Moscatelli, Guillermo
Moroni, Samanta
Chabay, Paola Andrea
Preciado, María Victoria
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Epstein Barr Virus
Lmp1
Ed-L1 Promoter
Lymphoma
Infectious Mononucleosis
Pediatric Patients
topic Epstein Barr Virus
Lmp1
Ed-L1 Promoter
Lymphoma
Infectious Mononucleosis
Pediatric Patients
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The Epstein Barr virus (EBV) is associated with several lymphoid and epithelial malignancies such as Hodgkin and Burkitt lymphoma or nasopharyngeal carcinoma and it is also the etiological agent of infectious mononucleosis (IM). Transcriptional regulation of the viral oncoprotein LMP1, remains yet not fully understood. LMP1 expression can be initiated in an EBNA2 dependent or independent manner from ED-L1 or LT-R1 promoters. It has been proposed that sequence variation at ED-L1 region could be an important factor concerning LMP1 expression. In order to characterize the natural sequence variation of the ED-L1 promoter, and its relationship with neoplasia, 44 pediatric patients, 17 IM and 27 EBV-associated lymphoma cases from Argentina, were studied. Phylogenetic analysis showed 4 main clusters, namely B95.8, Raji, Cao and P3HR1. Most isolates, 80.3%, conformed the B95.8 group. Co-infection with more than one viral variant was detected in 5/17 IM cases, but no co-infections were detected among lymphoma cases. Moreover, co-infected IM cases exhibited differences between the ED-L1 sequences obtained from different anatomical compartments. Mutations confined to transcription factor binding sites such as SP1/SP3, CRE, AP2, C/EBP were found in similar proportions in 23 isolates from both benign and malignant samples, rendering the distribution of these mutations not significant among malignant samples.
Fil: Gantuz, Magdalena. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lorenzetti, Mario Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moscatelli, Guillermo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moroni, Samanta. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina
Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Preciado, María Victoria. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The Epstein Barr virus (EBV) is associated with several lymphoid and epithelial malignancies such as Hodgkin and Burkitt lymphoma or nasopharyngeal carcinoma and it is also the etiological agent of infectious mononucleosis (IM). Transcriptional regulation of the viral oncoprotein LMP1, remains yet not fully understood. LMP1 expression can be initiated in an EBNA2 dependent or independent manner from ED-L1 or LT-R1 promoters. It has been proposed that sequence variation at ED-L1 region could be an important factor concerning LMP1 expression. In order to characterize the natural sequence variation of the ED-L1 promoter, and its relationship with neoplasia, 44 pediatric patients, 17 IM and 27 EBV-associated lymphoma cases from Argentina, were studied. Phylogenetic analysis showed 4 main clusters, namely B95.8, Raji, Cao and P3HR1. Most isolates, 80.3%, conformed the B95.8 group. Co-infection with more than one viral variant was detected in 5/17 IM cases, but no co-infections were detected among lymphoma cases. Moreover, co-infected IM cases exhibited differences between the ED-L1 sequences obtained from different anatomical compartments. Mutations confined to transcription factor binding sites such as SP1/SP3, CRE, AP2, C/EBP were found in similar proportions in 23 isolates from both benign and malignant samples, rendering the distribution of these mutations not significant among malignant samples.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23963
Gantuz, Magdalena; Lorenzetti, Mario Alejandro; Altcheh, Jaime Marcelo; de Matteo, Elena Noemí; Moscatelli, Guillermo; et al.; LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina; Elsevier; Infection, Genetics and Evolution; 14; 1-2013; 275-281
1567-1348
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23963
identifier_str_mv Gantuz, Magdalena; Lorenzetti, Mario Alejandro; Altcheh, Jaime Marcelo; de Matteo, Elena Noemí; Moscatelli, Guillermo; et al.; LMP1 promoter sequence analysis in Epstein Barr virus pediatric infection reveals preferential circulation of B95.8 related variants in Argentina; Elsevier; Infection, Genetics and Evolution; 14; 1-2013; 275-281
1567-1348
CONICET Digital
CONICET
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language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2012.12.025
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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