Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model

Autores
Cicora, F.; Stringa, Pablo Luis; Guerrieri, Diego; Roberti, J.; Ambrosi, Nella Gabriela; Toniolo, F.; Cicora, P.; Palti, G.; Vasquez, D.; Raimondi, C.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Brain death (BD), a non-immunological factor of renal injury, triggers an inflammatory process causing pathological signs of cell death in the kidney, such as necrosis and apoptosis. Kidneys from brain dead donors show lower success rates than kidneys from living donors and one strategy to improve transplantation outcome is to precondition the donors. For the first time, anti-rat thymoglobulin (rATG) was administered in an experimental brain death animal model to evaluate if it could ameliorate histopathological damage and improve organ function. Animals were divided into three groups: V (n=5) ventilated for 2h; BD (n=5) brain death and ventilated for 2h; and BD+rATG (n=5) brain death, ventilated for 2h, rATG was administered during brain death (10mg/kg). We observed lower creatinine levels in treatment groups (means): V, 0·88±0·22mg/dl; BD, 1·37±0·07mg/dl; and BD+rATG, 0·64±0·02mg/dl (BD versus BD+rATG, P<0·001). In the BD group there appeared to be a marked increase of ATN, whereas ATN was decreased significantly in the rATG group (V, 2·25±0·5 versus BD, 4·75±0·5, P<0·01; BD+rATG, 2·75±0·5 versus BD 4·75±0·5 P<0·01). Gene expression was evaluated with reverse transcription-polymerase chain reaction; tumour necrosis factor (TNF)-α, interleukin (IL)-6, C3, CD86 showed no significant difference between groups. Increased IL-10 and decreased CCL2 in BD+rATG compared to BD (both cases P<0·01). Myeloperoxidase was increased significantly after the brain death setting (V: 32±7·5 versus BD: 129±18). Findings suggest that rATG administered to potential donors may ameliorate renal damage caused by BD. These findings could contribute in the search for specific cytoprotective interventions to improve the quality and viability of transplanted organs.
Fil: Cicora, F.. Hospital Alemán; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Stringa, Pablo Luis. Universidad Nacional de La Plata; Argentina. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Roberti, J.. No especifíca;
Fil: Ambrosi, Nella Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Toniolo, F.. No especifíca;
Fil: Cicora, P.. No especifíca;
Fil: Palti, G.. Hospital Alemán; Argentina
Fil: Vasquez, D.. No especifíca;
Fil: Raimondi, C.. Universidad Nacional de La Plata; Argentina
Materia
ACUTE TUBULAR NECROSIS
BRAIN DEATH
IL-10
MCP-1
THYMOGLOBULIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197582
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/197582
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat modelCicora, F.Stringa, Pablo LuisGuerrieri, DiegoRoberti, J.Ambrosi, Nella GabrielaToniolo, F.Cicora, P.Palti, G.Vasquez, D.Raimondi, C.ACUTE TUBULAR NECROSISBRAIN DEATHIL-10MCP-1THYMOGLOBULINhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Brain death (BD), a non-immunological factor of renal injury, triggers an inflammatory process causing pathological signs of cell death in the kidney, such as necrosis and apoptosis. Kidneys from brain dead donors show lower success rates than kidneys from living donors and one strategy to improve transplantation outcome is to precondition the donors. For the first time, anti-rat thymoglobulin (rATG) was administered in an experimental brain death animal model to evaluate if it could ameliorate histopathological damage and improve organ function. Animals were divided into three groups: V (n=5) ventilated for 2h; BD (n=5) brain death and ventilated for 2h; and BD+rATG (n=5) brain death, ventilated for 2h, rATG was administered during brain death (10mg/kg). We observed lower creatinine levels in treatment groups (means): V, 0·88±0·22mg/dl; BD, 1·37±0·07mg/dl; and BD+rATG, 0·64±0·02mg/dl (BD versus BD+rATG, P<0·001). In the BD group there appeared to be a marked increase of ATN, whereas ATN was decreased significantly in the rATG group (V, 2·25±0·5 versus BD, 4·75±0·5, P<0·01; BD+rATG, 2·75±0·5 versus BD 4·75±0·5 P<0·01). Gene expression was evaluated with reverse transcription-polymerase chain reaction; tumour necrosis factor (TNF)-α, interleukin (IL)-6, C3, CD86 showed no significant difference between groups. Increased IL-10 and decreased CCL2 in BD+rATG compared to BD (both cases P<0·01). Myeloperoxidase was increased significantly after the brain death setting (V: 32±7·5 versus BD: 129±18). Findings suggest that rATG administered to potential donors may ameliorate renal damage caused by BD. These findings could contribute in the search for specific cytoprotective interventions to improve the quality and viability of transplanted organs.Fil: Cicora, F.. Hospital Alemán; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Stringa, Pablo Luis. Universidad Nacional de La Plata; Argentina. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Roberti, J.. No especifíca;Fil: Ambrosi, Nella Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Toniolo, F.. No especifíca;Fil: Cicora, P.. No especifíca;Fil: Palti, G.. Hospital Alemán; ArgentinaFil: Vasquez, D.. No especifíca;Fil: Raimondi, C.. Universidad Nacional de La Plata; ArgentinaWiley Blackwell Publishing, Inc2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197582Cicora, F.; Stringa, Pablo Luis; Guerrieri, Diego; Roberti, J.; Ambrosi, Nella Gabriela; et al.; Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model; Wiley Blackwell Publishing, Inc; Clinical and Experimental Immunology; 169; 3; 9-2012; 330-3370009-9104CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/cei/article/169/3/330/6438577?login=falseinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2249.2012.04617.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:49Zoai:ri.conicet.gov.ar:11336/197582instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:49.522CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model
title Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model
spellingShingle Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model
Cicora, F.
ACUTE TUBULAR NECROSIS
BRAIN DEATH
IL-10
MCP-1
THYMOGLOBULIN
title_short Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model
title_full Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model
title_fullStr Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model
title_full_unstemmed Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model
title_sort Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model
dc.creator.none.fl_str_mv Cicora, F.
Stringa, Pablo Luis
Guerrieri, Diego
Roberti, J.
Ambrosi, Nella Gabriela
Toniolo, F.
Cicora, P.
Palti, G.
Vasquez, D.
Raimondi, C.
author Cicora, F.
author_facet Cicora, F.
Stringa, Pablo Luis
Guerrieri, Diego
Roberti, J.
Ambrosi, Nella Gabriela
Toniolo, F.
Cicora, P.
Palti, G.
Vasquez, D.
Raimondi, C.
author_role author
author2 Stringa, Pablo Luis
Guerrieri, Diego
Roberti, J.
Ambrosi, Nella Gabriela
Toniolo, F.
Cicora, P.
Palti, G.
Vasquez, D.
Raimondi, C.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACUTE TUBULAR NECROSIS
BRAIN DEATH
IL-10
MCP-1
THYMOGLOBULIN
topic ACUTE TUBULAR NECROSIS
BRAIN DEATH
IL-10
MCP-1
THYMOGLOBULIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Brain death (BD), a non-immunological factor of renal injury, triggers an inflammatory process causing pathological signs of cell death in the kidney, such as necrosis and apoptosis. Kidneys from brain dead donors show lower success rates than kidneys from living donors and one strategy to improve transplantation outcome is to precondition the donors. For the first time, anti-rat thymoglobulin (rATG) was administered in an experimental brain death animal model to evaluate if it could ameliorate histopathological damage and improve organ function. Animals were divided into three groups: V (n=5) ventilated for 2h; BD (n=5) brain death and ventilated for 2h; and BD+rATG (n=5) brain death, ventilated for 2h, rATG was administered during brain death (10mg/kg). We observed lower creatinine levels in treatment groups (means): V, 0·88±0·22mg/dl; BD, 1·37±0·07mg/dl; and BD+rATG, 0·64±0·02mg/dl (BD versus BD+rATG, P<0·001). In the BD group there appeared to be a marked increase of ATN, whereas ATN was decreased significantly in the rATG group (V, 2·25±0·5 versus BD, 4·75±0·5, P<0·01; BD+rATG, 2·75±0·5 versus BD 4·75±0·5 P<0·01). Gene expression was evaluated with reverse transcription-polymerase chain reaction; tumour necrosis factor (TNF)-α, interleukin (IL)-6, C3, CD86 showed no significant difference between groups. Increased IL-10 and decreased CCL2 in BD+rATG compared to BD (both cases P<0·01). Myeloperoxidase was increased significantly after the brain death setting (V: 32±7·5 versus BD: 129±18). Findings suggest that rATG administered to potential donors may ameliorate renal damage caused by BD. These findings could contribute in the search for specific cytoprotective interventions to improve the quality and viability of transplanted organs.
Fil: Cicora, F.. Hospital Alemán; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Stringa, Pablo Luis. Universidad Nacional de La Plata; Argentina. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Roberti, J.. No especifíca;
Fil: Ambrosi, Nella Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Toniolo, F.. No especifíca;
Fil: Cicora, P.. No especifíca;
Fil: Palti, G.. Hospital Alemán; Argentina
Fil: Vasquez, D.. No especifíca;
Fil: Raimondi, C.. Universidad Nacional de La Plata; Argentina
description Brain death (BD), a non-immunological factor of renal injury, triggers an inflammatory process causing pathological signs of cell death in the kidney, such as necrosis and apoptosis. Kidneys from brain dead donors show lower success rates than kidneys from living donors and one strategy to improve transplantation outcome is to precondition the donors. For the first time, anti-rat thymoglobulin (rATG) was administered in an experimental brain death animal model to evaluate if it could ameliorate histopathological damage and improve organ function. Animals were divided into three groups: V (n=5) ventilated for 2h; BD (n=5) brain death and ventilated for 2h; and BD+rATG (n=5) brain death, ventilated for 2h, rATG was administered during brain death (10mg/kg). We observed lower creatinine levels in treatment groups (means): V, 0·88±0·22mg/dl; BD, 1·37±0·07mg/dl; and BD+rATG, 0·64±0·02mg/dl (BD versus BD+rATG, P<0·001). In the BD group there appeared to be a marked increase of ATN, whereas ATN was decreased significantly in the rATG group (V, 2·25±0·5 versus BD, 4·75±0·5, P<0·01; BD+rATG, 2·75±0·5 versus BD 4·75±0·5 P<0·01). Gene expression was evaluated with reverse transcription-polymerase chain reaction; tumour necrosis factor (TNF)-α, interleukin (IL)-6, C3, CD86 showed no significant difference between groups. Increased IL-10 and decreased CCL2 in BD+rATG compared to BD (both cases P<0·01). Myeloperoxidase was increased significantly after the brain death setting (V: 32±7·5 versus BD: 129±18). Findings suggest that rATG administered to potential donors may ameliorate renal damage caused by BD. These findings could contribute in the search for specific cytoprotective interventions to improve the quality and viability of transplanted organs.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197582
Cicora, F.; Stringa, Pablo Luis; Guerrieri, Diego; Roberti, J.; Ambrosi, Nella Gabriela; et al.; Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model; Wiley Blackwell Publishing, Inc; Clinical and Experimental Immunology; 169; 3; 9-2012; 330-337
0009-9104
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197582
identifier_str_mv Cicora, F.; Stringa, Pablo Luis; Guerrieri, Diego; Roberti, J.; Ambrosi, Nella Gabriela; et al.; Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model; Wiley Blackwell Publishing, Inc; Clinical and Experimental Immunology; 169; 3; 9-2012; 330-337
0009-9104
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/cei/article/169/3/330/6438577?login=false
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2249.2012.04617.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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