Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis
- Autores
- Uribe, Pamela; Cabrillana, María Eugenia; Fornes, Miguel Walter; Treulen, Favián; Boguen, Rodrigo; Isachenko, Vladimir; Isachenko, Evgenia; Sánchez, Raúl; Villegas, Juana V.
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Peroxynitrite is a highly reactive nitrogen species and a potent inducer of apoptosis and necrosis in somatic cells. Peroxynitrite-induced nitrosative stress has emerged as a major cause of impaired sperm function; however, its ability to trigger cell death has not been described in human spermatozoa. The objective here was to characterize biochemical and morphological features of cell death induced by peroxynitrite-mediated nitrosative stress in human spermatozoa. For this, spermatozoa were incubated with and without (untreated control) 3-morpholinosydnonimine (SIN-1), in order to generate peroxynitrite. Sperm viability, mitochondrial permeability transition (MPT), externalization of phosphatidylserine, DNA oxidation and fragmentation, caspase activation, tyrosine nitration, and sperm ultrastructure were analyzed. The results showed that at 24 h of incubation with SIN-1, the sperm viability was significantly reduced compared to untreated control (P < 0.001). Furthermore, the MPT was induced (P < 0.01) and increment in DNA oxidation (P < 0.01), DNA fragmentation (P < 0.01), tyrosine nitration (P < 0.0001) and ultrastructural damage were observed when compared to untreated control. Caspase activation was not evidenced, and although phosphatidylserine externalization increased compared to untreated control (P < 0.001), this process was observed in <10% of the cells and the gradual loss of viability was not characterized by an important increase in this parameter. In conclusion, peroxynitrite-mediated nitrosative stress induces the regulated variant of cell death known as MPT-driven necrosis in human spermatozoa. This study provides a new insight into the pathophysiology of nitrosative stress in human spermatozoa and opens up a new focus for developing specific therapeutic strategies to better preserve sperm viability or to avoid cell death.
Fil: Uribe, Pamela. Universidad de La Frontera; Chile
Fil: Cabrillana, María Eugenia. Aconcagua University; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Fornes, Miguel Walter. Aconcagua University; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Treulen, Favián. Universidad de La Frontera; Chile
Fil: Boguen, Rodrigo. Universidad de La Frontera; Chile
Fil: Isachenko, Vladimir. Universität zu Berlin; Alemania
Fil: Isachenko, Evgenia. Universitat zu Köln; Alemania
Fil: Sánchez, Raúl. Universidad de La Frontera; Chile
Fil: Villegas, Juana V.. Universidad de La Frontera; Chile - Materia
-
NECROSIS
NITROSATIVE STRESS
OXIDATIVE STRESS
PEROXYNITRITE
SPERM CELL DEATH - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/92636
Ver los metadatos del registro completo
id |
CONICETDig_49e5a3b4aea6cf34050eb0a324a1a2d3 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/92636 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosisUribe, PamelaCabrillana, María EugeniaFornes, Miguel WalterTreulen, FaviánBoguen, RodrigoIsachenko, VladimirIsachenko, EvgeniaSánchez, RaúlVillegas, Juana V.NECROSISNITROSATIVE STRESSOXIDATIVE STRESSPEROXYNITRITESPERM CELL DEATHhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Peroxynitrite is a highly reactive nitrogen species and a potent inducer of apoptosis and necrosis in somatic cells. Peroxynitrite-induced nitrosative stress has emerged as a major cause of impaired sperm function; however, its ability to trigger cell death has not been described in human spermatozoa. The objective here was to characterize biochemical and morphological features of cell death induced by peroxynitrite-mediated nitrosative stress in human spermatozoa. For this, spermatozoa were incubated with and without (untreated control) 3-morpholinosydnonimine (SIN-1), in order to generate peroxynitrite. Sperm viability, mitochondrial permeability transition (MPT), externalization of phosphatidylserine, DNA oxidation and fragmentation, caspase activation, tyrosine nitration, and sperm ultrastructure were analyzed. The results showed that at 24 h of incubation with SIN-1, the sperm viability was significantly reduced compared to untreated control (P < 0.001). Furthermore, the MPT was induced (P < 0.01) and increment in DNA oxidation (P < 0.01), DNA fragmentation (P < 0.01), tyrosine nitration (P < 0.0001) and ultrastructural damage were observed when compared to untreated control. Caspase activation was not evidenced, and although phosphatidylserine externalization increased compared to untreated control (P < 0.001), this process was observed in <10% of the cells and the gradual loss of viability was not characterized by an important increase in this parameter. In conclusion, peroxynitrite-mediated nitrosative stress induces the regulated variant of cell death known as MPT-driven necrosis in human spermatozoa. This study provides a new insight into the pathophysiology of nitrosative stress in human spermatozoa and opens up a new focus for developing specific therapeutic strategies to better preserve sperm viability or to avoid cell death.Fil: Uribe, Pamela. Universidad de La Frontera; ChileFil: Cabrillana, María Eugenia. Aconcagua University; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Fornes, Miguel Walter. Aconcagua University; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Treulen, Favián. Universidad de La Frontera; ChileFil: Boguen, Rodrigo. Universidad de La Frontera; ChileFil: Isachenko, Vladimir. Universität zu Berlin; AlemaniaFil: Isachenko, Evgenia. Universitat zu Köln; AlemaniaFil: Sánchez, Raúl. Universidad de La Frontera; ChileFil: Villegas, Juana V.. Universidad de La Frontera; ChileAsian Society of Andrology2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92636Uribe, Pamela; Cabrillana, María Eugenia; Fornes, Miguel Walter; Treulen, Favián; Boguen, Rodrigo; et al.; Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis; Asian Society of Andrology; Asian Journal of Andrology; 20; 6; 11-2018; 600-6071008-682XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4103/aja.aja_29_18info:eu-repo/semantics/altIdentifier/url/http://www.ajandrology.com/article.asp?issn=1008-682X;year=2018;volume=20;issue=6;spage=600;epage=607;aulast=Uribeinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:24Zoai:ri.conicet.gov.ar:11336/92636instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:24.68CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis |
title |
Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis |
spellingShingle |
Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis Uribe, Pamela NECROSIS NITROSATIVE STRESS OXIDATIVE STRESS PEROXYNITRITE SPERM CELL DEATH |
title_short |
Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis |
title_full |
Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis |
title_fullStr |
Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis |
title_full_unstemmed |
Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis |
title_sort |
Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis |
dc.creator.none.fl_str_mv |
Uribe, Pamela Cabrillana, María Eugenia Fornes, Miguel Walter Treulen, Favián Boguen, Rodrigo Isachenko, Vladimir Isachenko, Evgenia Sánchez, Raúl Villegas, Juana V. |
author |
Uribe, Pamela |
author_facet |
Uribe, Pamela Cabrillana, María Eugenia Fornes, Miguel Walter Treulen, Favián Boguen, Rodrigo Isachenko, Vladimir Isachenko, Evgenia Sánchez, Raúl Villegas, Juana V. |
author_role |
author |
author2 |
Cabrillana, María Eugenia Fornes, Miguel Walter Treulen, Favián Boguen, Rodrigo Isachenko, Vladimir Isachenko, Evgenia Sánchez, Raúl Villegas, Juana V. |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
NECROSIS NITROSATIVE STRESS OXIDATIVE STRESS PEROXYNITRITE SPERM CELL DEATH |
topic |
NECROSIS NITROSATIVE STRESS OXIDATIVE STRESS PEROXYNITRITE SPERM CELL DEATH |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Peroxynitrite is a highly reactive nitrogen species and a potent inducer of apoptosis and necrosis in somatic cells. Peroxynitrite-induced nitrosative stress has emerged as a major cause of impaired sperm function; however, its ability to trigger cell death has not been described in human spermatozoa. The objective here was to characterize biochemical and morphological features of cell death induced by peroxynitrite-mediated nitrosative stress in human spermatozoa. For this, spermatozoa were incubated with and without (untreated control) 3-morpholinosydnonimine (SIN-1), in order to generate peroxynitrite. Sperm viability, mitochondrial permeability transition (MPT), externalization of phosphatidylserine, DNA oxidation and fragmentation, caspase activation, tyrosine nitration, and sperm ultrastructure were analyzed. The results showed that at 24 h of incubation with SIN-1, the sperm viability was significantly reduced compared to untreated control (P < 0.001). Furthermore, the MPT was induced (P < 0.01) and increment in DNA oxidation (P < 0.01), DNA fragmentation (P < 0.01), tyrosine nitration (P < 0.0001) and ultrastructural damage were observed when compared to untreated control. Caspase activation was not evidenced, and although phosphatidylserine externalization increased compared to untreated control (P < 0.001), this process was observed in <10% of the cells and the gradual loss of viability was not characterized by an important increase in this parameter. In conclusion, peroxynitrite-mediated nitrosative stress induces the regulated variant of cell death known as MPT-driven necrosis in human spermatozoa. This study provides a new insight into the pathophysiology of nitrosative stress in human spermatozoa and opens up a new focus for developing specific therapeutic strategies to better preserve sperm viability or to avoid cell death. Fil: Uribe, Pamela. Universidad de La Frontera; Chile Fil: Cabrillana, María Eugenia. Aconcagua University; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Fornes, Miguel Walter. Aconcagua University; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Treulen, Favián. Universidad de La Frontera; Chile Fil: Boguen, Rodrigo. Universidad de La Frontera; Chile Fil: Isachenko, Vladimir. Universität zu Berlin; Alemania Fil: Isachenko, Evgenia. Universitat zu Köln; Alemania Fil: Sánchez, Raúl. Universidad de La Frontera; Chile Fil: Villegas, Juana V.. Universidad de La Frontera; Chile |
description |
Peroxynitrite is a highly reactive nitrogen species and a potent inducer of apoptosis and necrosis in somatic cells. Peroxynitrite-induced nitrosative stress has emerged as a major cause of impaired sperm function; however, its ability to trigger cell death has not been described in human spermatozoa. The objective here was to characterize biochemical and morphological features of cell death induced by peroxynitrite-mediated nitrosative stress in human spermatozoa. For this, spermatozoa were incubated with and without (untreated control) 3-morpholinosydnonimine (SIN-1), in order to generate peroxynitrite. Sperm viability, mitochondrial permeability transition (MPT), externalization of phosphatidylserine, DNA oxidation and fragmentation, caspase activation, tyrosine nitration, and sperm ultrastructure were analyzed. The results showed that at 24 h of incubation with SIN-1, the sperm viability was significantly reduced compared to untreated control (P < 0.001). Furthermore, the MPT was induced (P < 0.01) and increment in DNA oxidation (P < 0.01), DNA fragmentation (P < 0.01), tyrosine nitration (P < 0.0001) and ultrastructural damage were observed when compared to untreated control. Caspase activation was not evidenced, and although phosphatidylserine externalization increased compared to untreated control (P < 0.001), this process was observed in <10% of the cells and the gradual loss of viability was not characterized by an important increase in this parameter. In conclusion, peroxynitrite-mediated nitrosative stress induces the regulated variant of cell death known as MPT-driven necrosis in human spermatozoa. This study provides a new insight into the pathophysiology of nitrosative stress in human spermatozoa and opens up a new focus for developing specific therapeutic strategies to better preserve sperm viability or to avoid cell death. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/92636 Uribe, Pamela; Cabrillana, María Eugenia; Fornes, Miguel Walter; Treulen, Favián; Boguen, Rodrigo; et al.; Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis; Asian Society of Andrology; Asian Journal of Andrology; 20; 6; 11-2018; 600-607 1008-682X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/92636 |
identifier_str_mv |
Uribe, Pamela; Cabrillana, María Eugenia; Fornes, Miguel Walter; Treulen, Favián; Boguen, Rodrigo; et al.; Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis; Asian Society of Andrology; Asian Journal of Andrology; 20; 6; 11-2018; 600-607 1008-682X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.4103/aja.aja_29_18 info:eu-repo/semantics/altIdentifier/url/http://www.ajandrology.com/article.asp?issn=1008-682X;year=2018;volume=20;issue=6;spage=600;epage=607;aulast=Uribe |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Asian Society of Andrology |
publisher.none.fl_str_mv |
Asian Society of Andrology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842270118338363392 |
score |
13.13397 |