Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model

Autores
Cicora, Federico; Stringa, Pablo Luis; Guerrieri, Diego; Vásquez, Daniela; Toniolo, Fernanda; Roberti, Javier; Raimondi, Clemente
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rabbit anti-rat thymoglobulin (rATG) administered to donors with brain death (BD) may improve organs quality. We explored the effects of rATG administered to BD donors in the histology of heart, lungs and small bowel in a rat experimental model. Animals were randomly assigned to 3 groups: V (n = 5) no BD, 2 h ventilation; BD (n = 5) BD and 2 h ventilation; BD and rATG: BD, 2 h ventilation, rATG (10 mg/kg) after BD diagnosis. Histopathological damage scores were based on neutrophil infiltration, airway epithelial cell damage, interstitial edema, hyaline membrane formation, and pulmonary hemorrhage (lungs); neutrophil infiltration and interstitial edema (heart); Park score (bowel). Lung damage was significantly lower in BD + rATG group: V 5 ± 1.6; BD 11.25 ± 0.5, BD + rATG 6.5 ± 1.9 (p < 0.01). Heart: V 2.0 ± 0.81; BD 4.75 ± 1.25 and BD + rATG 3.5 ± 1.7 (p > 0.05). Small bowel: BD 2.25 ± 0.96 vs. BD + rATG 1.00 ± 1.15 (n.s.). Histological damage amelioration in lung and attenuation tendency in heart and small bowel encourages research of cytoprotective strategies to improve organ viability.
Fil: Cicora, Federico. Foundation for Research & Assistance in Kidney Disease; Argentina. Hospital Alemán; Argentina
Fil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Vásquez, Daniela. Foundation for Research & Assistance in Kidney Disease; Argentina
Fil: Toniolo, Fernanda. Universidad Nacional de La Plata; Argentina
Fil: Roberti, Javier. Foundation for Research & Assistance in Kidney Disease; Argentina
Fil: Raimondi, Clemente. Universidad Nacional de La Plata; Argentina
Materia
Transplantation
Donor Preconditioning
Animal Model
Thymoglobulin
Brain Death
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13994
Acceder
id CONICETDig_2cbe6a858e8583afdb3e57aaac12ce8b
oai_identifier_str oai:ri.conicet.gov.ar:11336/13994
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat modelCicora, FedericoStringa, Pablo LuisGuerrieri, DiegoVásquez, DanielaToniolo, FernandaRoberti, JavierRaimondi, ClementeTransplantationDonor PreconditioningAnimal ModelThymoglobulinBrain Deathhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Rabbit anti-rat thymoglobulin (rATG) administered to donors with brain death (BD) may improve organs quality. We explored the effects of rATG administered to BD donors in the histology of heart, lungs and small bowel in a rat experimental model. Animals were randomly assigned to 3 groups: V (n = 5) no BD, 2 h ventilation; BD (n = 5) BD and 2 h ventilation; BD and rATG: BD, 2 h ventilation, rATG (10 mg/kg) after BD diagnosis. Histopathological damage scores were based on neutrophil infiltration, airway epithelial cell damage, interstitial edema, hyaline membrane formation, and pulmonary hemorrhage (lungs); neutrophil infiltration and interstitial edema (heart); Park score (bowel). Lung damage was significantly lower in BD + rATG group: V 5 ± 1.6; BD 11.25 ± 0.5, BD + rATG 6.5 ± 1.9 (p < 0.01). Heart: V 2.0 ± 0.81; BD 4.75 ± 1.25 and BD + rATG 3.5 ± 1.7 (p > 0.05). Small bowel: BD 2.25 ± 0.96 vs. BD + rATG 1.00 ± 1.15 (n.s.). Histological damage amelioration in lung and attenuation tendency in heart and small bowel encourages research of cytoprotective strategies to improve organ viability.Fil: Cicora, Federico. Foundation for Research & Assistance in Kidney Disease; Argentina. Hospital Alemán; ArgentinaFil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Vásquez, Daniela. Foundation for Research & Assistance in Kidney Disease; ArgentinaFil: Toniolo, Fernanda. Universidad Nacional de La Plata; ArgentinaFil: Roberti, Javier. Foundation for Research & Assistance in Kidney Disease; ArgentinaFil: Raimondi, Clemente. Universidad Nacional de La Plata; ArgentinaElsevier Science2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13994Cicora, Federico; Stringa, Pablo Luis; Guerrieri, Diego; Vásquez, Daniela; Toniolo, Fernanda; et al.; Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model; Elsevier Science; Transplant Immunology; 28; 4; 6-2013; 203-2050966-3274enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S096632741300021Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.trim.2013.04.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:46Zoai:ri.conicet.gov.ar:11336/13994instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:46.688CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model
title Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model
spellingShingle Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model
Cicora, Federico
Transplantation
Donor Preconditioning
Animal Model
Thymoglobulin
Brain Death
title_short Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model
title_full Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model
title_fullStr Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model
title_full_unstemmed Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model
title_sort Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model
dc.creator.none.fl_str_mv Cicora, Federico
Stringa, Pablo Luis
Guerrieri, Diego
Vásquez, Daniela
Toniolo, Fernanda
Roberti, Javier
Raimondi, Clemente
author Cicora, Federico
author_facet Cicora, Federico
Stringa, Pablo Luis
Guerrieri, Diego
Vásquez, Daniela
Toniolo, Fernanda
Roberti, Javier
Raimondi, Clemente
author_role author
author2 Stringa, Pablo Luis
Guerrieri, Diego
Vásquez, Daniela
Toniolo, Fernanda
Roberti, Javier
Raimondi, Clemente
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Transplantation
Donor Preconditioning
Animal Model
Thymoglobulin
Brain Death
topic Transplantation
Donor Preconditioning
Animal Model
Thymoglobulin
Brain Death
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Rabbit anti-rat thymoglobulin (rATG) administered to donors with brain death (BD) may improve organs quality. We explored the effects of rATG administered to BD donors in the histology of heart, lungs and small bowel in a rat experimental model. Animals were randomly assigned to 3 groups: V (n = 5) no BD, 2 h ventilation; BD (n = 5) BD and 2 h ventilation; BD and rATG: BD, 2 h ventilation, rATG (10 mg/kg) after BD diagnosis. Histopathological damage scores were based on neutrophil infiltration, airway epithelial cell damage, interstitial edema, hyaline membrane formation, and pulmonary hemorrhage (lungs); neutrophil infiltration and interstitial edema (heart); Park score (bowel). Lung damage was significantly lower in BD + rATG group: V 5 ± 1.6; BD 11.25 ± 0.5, BD + rATG 6.5 ± 1.9 (p < 0.01). Heart: V 2.0 ± 0.81; BD 4.75 ± 1.25 and BD + rATG 3.5 ± 1.7 (p > 0.05). Small bowel: BD 2.25 ± 0.96 vs. BD + rATG 1.00 ± 1.15 (n.s.). Histological damage amelioration in lung and attenuation tendency in heart and small bowel encourages research of cytoprotective strategies to improve organ viability.
Fil: Cicora, Federico. Foundation for Research & Assistance in Kidney Disease; Argentina. Hospital Alemán; Argentina
Fil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Vásquez, Daniela. Foundation for Research & Assistance in Kidney Disease; Argentina
Fil: Toniolo, Fernanda. Universidad Nacional de La Plata; Argentina
Fil: Roberti, Javier. Foundation for Research & Assistance in Kidney Disease; Argentina
Fil: Raimondi, Clemente. Universidad Nacional de La Plata; Argentina
description Rabbit anti-rat thymoglobulin (rATG) administered to donors with brain death (BD) may improve organs quality. We explored the effects of rATG administered to BD donors in the histology of heart, lungs and small bowel in a rat experimental model. Animals were randomly assigned to 3 groups: V (n = 5) no BD, 2 h ventilation; BD (n = 5) BD and 2 h ventilation; BD and rATG: BD, 2 h ventilation, rATG (10 mg/kg) after BD diagnosis. Histopathological damage scores were based on neutrophil infiltration, airway epithelial cell damage, interstitial edema, hyaline membrane formation, and pulmonary hemorrhage (lungs); neutrophil infiltration and interstitial edema (heart); Park score (bowel). Lung damage was significantly lower in BD + rATG group: V 5 ± 1.6; BD 11.25 ± 0.5, BD + rATG 6.5 ± 1.9 (p < 0.01). Heart: V 2.0 ± 0.81; BD 4.75 ± 1.25 and BD + rATG 3.5 ± 1.7 (p > 0.05). Small bowel: BD 2.25 ± 0.96 vs. BD + rATG 1.00 ± 1.15 (n.s.). Histological damage amelioration in lung and attenuation tendency in heart and small bowel encourages research of cytoprotective strategies to improve organ viability.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13994
Cicora, Federico; Stringa, Pablo Luis; Guerrieri, Diego; Vásquez, Daniela; Toniolo, Fernanda; et al.; Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model; Elsevier Science; Transplant Immunology; 28; 4; 6-2013; 203-205
0966-3274
url http://hdl.handle.net/11336/13994
identifier_str_mv Cicora, Federico; Stringa, Pablo Luis; Guerrieri, Diego; Vásquez, Daniela; Toniolo, Fernanda; et al.; Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model; Elsevier Science; Transplant Immunology; 28; 4; 6-2013; 203-205
0966-3274
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S096632741300021X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.trim.2013.04.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269181119037440
score 13.13397

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