Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine

Autores
Perrotta, Patricio Rene; Arevalo, Fernando Javier; Vettorazzi, Nelio Roberto; Zon, María Alicia; Fernández, Héctor
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An electrochemical immunosensor (EI) for the determination of ochratoxin A (OTA) in red wine samples was developed. This immunosensor was based on Protein G functionalized magnetic beads (MBs) as solid phase for affinity reaction between OTA and OTA monoclonal antibody (mAbOTA). A carbon screen printed-electrodes (CSPE) system was used as electrochemical transduction element. This immunosensor was based on a direct competitive assay between OTA in wine samples and OTA labeled with horseradish peroxidase (HRP) (OTA-HRP). The HRP, in the presence of hydrogen peroxide catalyzes the oxidation of pyrocatechol to benzoquinone, whose back electrochemical reduction was detected on a CSPE by square wave voltammetry (SWV). The experimental variables involved in the immunosensor response to OTA were evaluated. The performance obtained for the EI was an analytical range of 0.01-20 ppb, limit of detection (LOD) of 0.008 ppb, and IC 50 = 0.272 ± 0.081 ppb. In addition, an acceptable accuracy with a relative standard deviation (RSD) of 5.56% and very good recoveries (92-110%) were found. This work shows the potential of our EI for the direct measurement of OTA in red wine samples combining SWV as electroanalytical technique with the MBs and CSPE. This EI has great advantages as direct measurement of red wine samples without a prior pretreatment, the small volume of sample, the short time consuming of experiences and LOD well below those established by the Regulatory Commission of the European Community (2 μg kg -1).
Fil: Perrotta, Patricio Rene. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química. Área Electroquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Vettorazzi, Nelio Roberto. Universidad Nacional de Río Cuarto; Argentina
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Fernández, Héctor. Universidad Nacional de Río Cuarto; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Materia
IMMUNOSENSOR
MAGNETIC BEADS
OCHRATOXIN A
SCREEN PRINTED-ELECTRODE
SQUARE WAVE VOLTAMMETRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/198109
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/198109
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red winePerrotta, Patricio ReneArevalo, Fernando JavierVettorazzi, Nelio RobertoZon, María AliciaFernández, HéctorIMMUNOSENSORMAGNETIC BEADSOCHRATOXIN ASCREEN PRINTED-ELECTRODESQUARE WAVE VOLTAMMETRYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An electrochemical immunosensor (EI) for the determination of ochratoxin A (OTA) in red wine samples was developed. This immunosensor was based on Protein G functionalized magnetic beads (MBs) as solid phase for affinity reaction between OTA and OTA monoclonal antibody (mAbOTA). A carbon screen printed-electrodes (CSPE) system was used as electrochemical transduction element. This immunosensor was based on a direct competitive assay between OTA in wine samples and OTA labeled with horseradish peroxidase (HRP) (OTA-HRP). The HRP, in the presence of hydrogen peroxide catalyzes the oxidation of pyrocatechol to benzoquinone, whose back electrochemical reduction was detected on a CSPE by square wave voltammetry (SWV). The experimental variables involved in the immunosensor response to OTA were evaluated. The performance obtained for the EI was an analytical range of 0.01-20 ppb, limit of detection (LOD) of 0.008 ppb, and IC 50 = 0.272 ± 0.081 ppb. In addition, an acceptable accuracy with a relative standard deviation (RSD) of 5.56% and very good recoveries (92-110%) were found. This work shows the potential of our EI for the direct measurement of OTA in red wine samples combining SWV as electroanalytical technique with the MBs and CSPE. This EI has great advantages as direct measurement of red wine samples without a prior pretreatment, the small volume of sample, the short time consuming of experiences and LOD well below those established by the Regulatory Commission of the European Community (2 μg kg -1).Fil: Perrotta, Patricio Rene. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química. Área Electroquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Vettorazzi, Nelio Roberto. Universidad Nacional de Río Cuarto; ArgentinaFil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Fernández, Héctor. Universidad Nacional de Río Cuarto; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaElsevier Science SA2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/198109Perrotta, Patricio Rene; Arevalo, Fernando Javier; Vettorazzi, Nelio Roberto; Zon, María Alicia; Fernández, Héctor; Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine; Elsevier Science SA; Sensors and Actuators B: Chemical; 162; 1; 2-2012; 327-3330925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2011.12.089info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925400511011798info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:13Zoai:ri.conicet.gov.ar:11336/198109instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:14.072CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine
title Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine
spellingShingle Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine
Perrotta, Patricio Rene
IMMUNOSENSOR
MAGNETIC BEADS
OCHRATOXIN A
SCREEN PRINTED-ELECTRODE
SQUARE WAVE VOLTAMMETRY
title_short Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine
title_full Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine
title_fullStr Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine
title_full_unstemmed Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine
title_sort Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine
dc.creator.none.fl_str_mv Perrotta, Patricio Rene
Arevalo, Fernando Javier
Vettorazzi, Nelio Roberto
Zon, María Alicia
Fernández, Héctor
author Perrotta, Patricio Rene
author_facet Perrotta, Patricio Rene
Arevalo, Fernando Javier
Vettorazzi, Nelio Roberto
Zon, María Alicia
Fernández, Héctor
author_role author
author2 Arevalo, Fernando Javier
Vettorazzi, Nelio Roberto
Zon, María Alicia
Fernández, Héctor
author2_role author
author
author
author
dc.subject.none.fl_str_mv IMMUNOSENSOR
MAGNETIC BEADS
OCHRATOXIN A
SCREEN PRINTED-ELECTRODE
SQUARE WAVE VOLTAMMETRY
topic IMMUNOSENSOR
MAGNETIC BEADS
OCHRATOXIN A
SCREEN PRINTED-ELECTRODE
SQUARE WAVE VOLTAMMETRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An electrochemical immunosensor (EI) for the determination of ochratoxin A (OTA) in red wine samples was developed. This immunosensor was based on Protein G functionalized magnetic beads (MBs) as solid phase for affinity reaction between OTA and OTA monoclonal antibody (mAbOTA). A carbon screen printed-electrodes (CSPE) system was used as electrochemical transduction element. This immunosensor was based on a direct competitive assay between OTA in wine samples and OTA labeled with horseradish peroxidase (HRP) (OTA-HRP). The HRP, in the presence of hydrogen peroxide catalyzes the oxidation of pyrocatechol to benzoquinone, whose back electrochemical reduction was detected on a CSPE by square wave voltammetry (SWV). The experimental variables involved in the immunosensor response to OTA were evaluated. The performance obtained for the EI was an analytical range of 0.01-20 ppb, limit of detection (LOD) of 0.008 ppb, and IC 50 = 0.272 ± 0.081 ppb. In addition, an acceptable accuracy with a relative standard deviation (RSD) of 5.56% and very good recoveries (92-110%) were found. This work shows the potential of our EI for the direct measurement of OTA in red wine samples combining SWV as electroanalytical technique with the MBs and CSPE. This EI has great advantages as direct measurement of red wine samples without a prior pretreatment, the small volume of sample, the short time consuming of experiences and LOD well below those established by the Regulatory Commission of the European Community (2 μg kg -1).
Fil: Perrotta, Patricio Rene. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química. Área Electroquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Vettorazzi, Nelio Roberto. Universidad Nacional de Río Cuarto; Argentina
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Fernández, Héctor. Universidad Nacional de Río Cuarto; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
description An electrochemical immunosensor (EI) for the determination of ochratoxin A (OTA) in red wine samples was developed. This immunosensor was based on Protein G functionalized magnetic beads (MBs) as solid phase for affinity reaction between OTA and OTA monoclonal antibody (mAbOTA). A carbon screen printed-electrodes (CSPE) system was used as electrochemical transduction element. This immunosensor was based on a direct competitive assay between OTA in wine samples and OTA labeled with horseradish peroxidase (HRP) (OTA-HRP). The HRP, in the presence of hydrogen peroxide catalyzes the oxidation of pyrocatechol to benzoquinone, whose back electrochemical reduction was detected on a CSPE by square wave voltammetry (SWV). The experimental variables involved in the immunosensor response to OTA were evaluated. The performance obtained for the EI was an analytical range of 0.01-20 ppb, limit of detection (LOD) of 0.008 ppb, and IC 50 = 0.272 ± 0.081 ppb. In addition, an acceptable accuracy with a relative standard deviation (RSD) of 5.56% and very good recoveries (92-110%) were found. This work shows the potential of our EI for the direct measurement of OTA in red wine samples combining SWV as electroanalytical technique with the MBs and CSPE. This EI has great advantages as direct measurement of red wine samples without a prior pretreatment, the small volume of sample, the short time consuming of experiences and LOD well below those established by the Regulatory Commission of the European Community (2 μg kg -1).
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/198109
Perrotta, Patricio Rene; Arevalo, Fernando Javier; Vettorazzi, Nelio Roberto; Zon, María Alicia; Fernández, Héctor; Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine; Elsevier Science SA; Sensors and Actuators B: Chemical; 162; 1; 2-2012; 327-333
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/198109
identifier_str_mv Perrotta, Patricio Rene; Arevalo, Fernando Javier; Vettorazzi, Nelio Roberto; Zon, María Alicia; Fernández, Héctor; Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine; Elsevier Science SA; Sensors and Actuators B: Chemical; 162; 1; 2-2012; 327-333
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2011.12.089
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925400511011798
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science SA
publisher.none.fl_str_mv Elsevier Science SA
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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