The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cells

Autores
Pronsato, Lucía; Milanesi, Lorena Magdalena; Vasconsuelo, Andrea Anahi; Frattini, Natalia María; Blanco, Nicolás Olegario
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rhabdomyosarcoma, a malignant tumor commonly found in children and adolescents, often carriesa poor prognosis. We conducted studies on the effects of the liposoluble extract of Nicotiana glauca (N. glauca), a plant from the Solanaceae family that is widely distributed, on the C2C12 murine myoblast cell line. We additionally investigated the antiproliferative effects of the extract on the RD rhabdomyosarcoma tumor cell line. The RD cells were pre-incubated without fetal bovine serum, followed by treatment with the liposoluble extract and the n-hexane sub-extract at specific concentrations. The effects on cell morphology were assessed using staining techniques. Cell viability, growth, and division capacities were evaluated through a wound assay. We analyzed the expression levels and subcellular localization of several proteins, including β-catenin, Notch 1, Caspase 3, and 14-3-3, using Western blotting. The extract of N. glauca induces apoptosis in C2C12 cells, suggesting that it may possess antitumor properties and could potentially be used in the treatment of hyperproliferative disorders. Our findings revealed that the control cells exhibited intact and normal nuclei, while the treated cells displayed characteristic morphological changes associated with apoptosis. Also, the treatments significantly reduced cell division capacity, andpotentially cell migration, compared to the control group. Moreover, the evaluation of β-catenin protein localization showed a cytoplasmic and membrane proximity in C2C12 cells, whereas RD cells exhibited nuclear localization. These findings suggest the potential therapeutic value of the extract in targeting the proliferation and spread of rhabdomyosarcoma cells.
Fil: Pronsato, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Milanesi, Lorena Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Vasconsuelo, Andrea Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Frattini, Natalia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Blanco, Nicolás Olegario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Materia
APOPTOSIS
RHABDOMYOSARCOMA CELLS
EXTRACTS OF N. GLAUCA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/274315
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/274315
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cellsPronsato, LucíaMilanesi, Lorena MagdalenaVasconsuelo, Andrea AnahiFrattini, Natalia MaríaBlanco, Nicolás OlegarioAPOPTOSISRHABDOMYOSARCOMA CELLSEXTRACTS OF N. GLAUCAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rhabdomyosarcoma, a malignant tumor commonly found in children and adolescents, often carriesa poor prognosis. We conducted studies on the effects of the liposoluble extract of Nicotiana glauca (N. glauca), a plant from the Solanaceae family that is widely distributed, on the C2C12 murine myoblast cell line. We additionally investigated the antiproliferative effects of the extract on the RD rhabdomyosarcoma tumor cell line. The RD cells were pre-incubated without fetal bovine serum, followed by treatment with the liposoluble extract and the n-hexane sub-extract at specific concentrations. The effects on cell morphology were assessed using staining techniques. Cell viability, growth, and division capacities were evaluated through a wound assay. We analyzed the expression levels and subcellular localization of several proteins, including β-catenin, Notch 1, Caspase 3, and 14-3-3, using Western blotting. The extract of N. glauca induces apoptosis in C2C12 cells, suggesting that it may possess antitumor properties and could potentially be used in the treatment of hyperproliferative disorders. Our findings revealed that the control cells exhibited intact and normal nuclei, while the treated cells displayed characteristic morphological changes associated with apoptosis. Also, the treatments significantly reduced cell division capacity, andpotentially cell migration, compared to the control group. Moreover, the evaluation of β-catenin protein localization showed a cytoplasmic and membrane proximity in C2C12 cells, whereas RD cells exhibited nuclear localization. These findings suggest the potential therapeutic value of the extract in targeting the proliferation and spread of rhabdomyosarcoma cells.Fil: Pronsato, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Milanesi, Lorena Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Vasconsuelo, Andrea Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Frattini, Natalia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Blanco, Nicolás Olegario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaProbiologist2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/274315Pronsato, Lucía; Milanesi, Lorena Magdalena; Vasconsuelo, Andrea Anahi; Frattini, Natalia María; Blanco, Nicolás Olegario; The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cells; Probiologist; Cell Signaling; 3; 1; 1-2025; 10-182837-8253CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.probiologists.com/article/the-extract-of-nicotiana-glauca-induces-apoptosis-in-rhabdomyosarcoma-cellsinfo:eu-repo/semantics/altIdentifier/doi/10.46439/signaling.3.049info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:42:05Zoai:ri.conicet.gov.ar:11336/274315instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:42:05.685CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cells
title The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cells
spellingShingle The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cells
Pronsato, Lucía
APOPTOSIS
RHABDOMYOSARCOMA CELLS
EXTRACTS OF N. GLAUCA
title_short The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cells
title_full The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cells
title_fullStr The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cells
title_full_unstemmed The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cells
title_sort The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cells
dc.creator.none.fl_str_mv Pronsato, Lucía
Milanesi, Lorena Magdalena
Vasconsuelo, Andrea Anahi
Frattini, Natalia María
Blanco, Nicolás Olegario
author Pronsato, Lucía
author_facet Pronsato, Lucía
Milanesi, Lorena Magdalena
Vasconsuelo, Andrea Anahi
Frattini, Natalia María
Blanco, Nicolás Olegario
author_role author
author2 Milanesi, Lorena Magdalena
Vasconsuelo, Andrea Anahi
Frattini, Natalia María
Blanco, Nicolás Olegario
author2_role author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
RHABDOMYOSARCOMA CELLS
EXTRACTS OF N. GLAUCA
topic APOPTOSIS
RHABDOMYOSARCOMA CELLS
EXTRACTS OF N. GLAUCA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rhabdomyosarcoma, a malignant tumor commonly found in children and adolescents, often carriesa poor prognosis. We conducted studies on the effects of the liposoluble extract of Nicotiana glauca (N. glauca), a plant from the Solanaceae family that is widely distributed, on the C2C12 murine myoblast cell line. We additionally investigated the antiproliferative effects of the extract on the RD rhabdomyosarcoma tumor cell line. The RD cells were pre-incubated without fetal bovine serum, followed by treatment with the liposoluble extract and the n-hexane sub-extract at specific concentrations. The effects on cell morphology were assessed using staining techniques. Cell viability, growth, and division capacities were evaluated through a wound assay. We analyzed the expression levels and subcellular localization of several proteins, including β-catenin, Notch 1, Caspase 3, and 14-3-3, using Western blotting. The extract of N. glauca induces apoptosis in C2C12 cells, suggesting that it may possess antitumor properties and could potentially be used in the treatment of hyperproliferative disorders. Our findings revealed that the control cells exhibited intact and normal nuclei, while the treated cells displayed characteristic morphological changes associated with apoptosis. Also, the treatments significantly reduced cell division capacity, andpotentially cell migration, compared to the control group. Moreover, the evaluation of β-catenin protein localization showed a cytoplasmic and membrane proximity in C2C12 cells, whereas RD cells exhibited nuclear localization. These findings suggest the potential therapeutic value of the extract in targeting the proliferation and spread of rhabdomyosarcoma cells.
Fil: Pronsato, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Milanesi, Lorena Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Vasconsuelo, Andrea Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Frattini, Natalia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Blanco, Nicolás Olegario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
description Rhabdomyosarcoma, a malignant tumor commonly found in children and adolescents, often carriesa poor prognosis. We conducted studies on the effects of the liposoluble extract of Nicotiana glauca (N. glauca), a plant from the Solanaceae family that is widely distributed, on the C2C12 murine myoblast cell line. We additionally investigated the antiproliferative effects of the extract on the RD rhabdomyosarcoma tumor cell line. The RD cells were pre-incubated without fetal bovine serum, followed by treatment with the liposoluble extract and the n-hexane sub-extract at specific concentrations. The effects on cell morphology were assessed using staining techniques. Cell viability, growth, and division capacities were evaluated through a wound assay. We analyzed the expression levels and subcellular localization of several proteins, including β-catenin, Notch 1, Caspase 3, and 14-3-3, using Western blotting. The extract of N. glauca induces apoptosis in C2C12 cells, suggesting that it may possess antitumor properties and could potentially be used in the treatment of hyperproliferative disorders. Our findings revealed that the control cells exhibited intact and normal nuclei, while the treated cells displayed characteristic morphological changes associated with apoptosis. Also, the treatments significantly reduced cell division capacity, andpotentially cell migration, compared to the control group. Moreover, the evaluation of β-catenin protein localization showed a cytoplasmic and membrane proximity in C2C12 cells, whereas RD cells exhibited nuclear localization. These findings suggest the potential therapeutic value of the extract in targeting the proliferation and spread of rhabdomyosarcoma cells.
publishDate 2025
dc.date.none.fl_str_mv 2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/274315
Pronsato, Lucía; Milanesi, Lorena Magdalena; Vasconsuelo, Andrea Anahi; Frattini, Natalia María; Blanco, Nicolás Olegario; The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cells; Probiologist; Cell Signaling; 3; 1; 1-2025; 10-18
2837-8253
CONICET Digital
CONICET
url http://hdl.handle.net/11336/274315
identifier_str_mv Pronsato, Lucía; Milanesi, Lorena Magdalena; Vasconsuelo, Andrea Anahi; Frattini, Natalia María; Blanco, Nicolás Olegario; The extract of Nicotiana glauca induces apoptosis in rhabdomyosarcoma cells; Probiologist; Cell Signaling; 3; 1; 1-2025; 10-18
2837-8253
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.probiologists.com/article/the-extract-of-nicotiana-glauca-induces-apoptosis-in-rhabdomyosarcoma-cells
info:eu-repo/semantics/altIdentifier/doi/10.46439/signaling.3.049
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Probiologist
publisher.none.fl_str_mv Probiologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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