Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis

Autores
Echeverría, Gustavo; Zumárraga, Martín José; Proaño Pérez, Freddy; Blasco, Francisco Barceló; de Waard, Jacobus H.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although several brands of tuberculin purifed protein derivatives (PPDs) are available for diagnosing bovine tuberculosis (bTB), comparative studies to determine their diagnostic accuracy are infrequent. In Ecuador we compared two diferent PPD brands for bTB diagnosis using skin testing and measuring skin thickness increase. Additionally, we evaluated four PPD brands, including those used for skin testing, in the Bovine Tuberculosis Interferon Gamma Test (IFN-γ test) measuring IFN-γ induction in whole blood. The study included 17 naturally tuberculosis-infected PPD and IFN-γ test positive bovines. Both the feld and laboratory results showed signifcant diferences in classifying the 17 bovines as bTB positive or negative. We hypothesize that several factors, such as the genetic background of the cows, sensitization to environmental mycobacteria, M. bovis strains involved in the bTB infection, and the manufacturing procedures of the PPDs, could have infuenced the immune reaction toward the diferent tuberculin PPD brands. Our study emphasizes the necessity for comparative studies aimed at determining the diagnostic accuracy of PPD brands for bTB diagnosis as well as the development of standardized methods for PPD production and potency determination.
Fil: Echeverría, Gustavo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Universidad Central del Ecuador; Ecuador
Fil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Proaño Pérez, Freddy. Universidad Central del Ecuador; Ecuador
Fil: Blasco, Francisco Barceló. Universidad de Las Américas.; Ecuador
Fil: de Waard, Jacobus H.. Universidad de Las Américas.; Ecuador
Materia
PPD
avian PPD
bovine PPD
IFN-y
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/261734
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosisEcheverría, GustavoZumárraga, Martín JoséProaño Pérez, FreddyBlasco, Francisco Barcelóde Waard, Jacobus H.PPDavian PPDbovine PPDIFN-yhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Although several brands of tuberculin purifed protein derivatives (PPDs) are available for diagnosing bovine tuberculosis (bTB), comparative studies to determine their diagnostic accuracy are infrequent. In Ecuador we compared two diferent PPD brands for bTB diagnosis using skin testing and measuring skin thickness increase. Additionally, we evaluated four PPD brands, including those used for skin testing, in the Bovine Tuberculosis Interferon Gamma Test (IFN-γ test) measuring IFN-γ induction in whole blood. The study included 17 naturally tuberculosis-infected PPD and IFN-γ test positive bovines. Both the feld and laboratory results showed signifcant diferences in classifying the 17 bovines as bTB positive or negative. We hypothesize that several factors, such as the genetic background of the cows, sensitization to environmental mycobacteria, M. bovis strains involved in the bTB infection, and the manufacturing procedures of the PPDs, could have infuenced the immune reaction toward the diferent tuberculin PPD brands. Our study emphasizes the necessity for comparative studies aimed at determining the diagnostic accuracy of PPD brands for bTB diagnosis as well as the development of standardized methods for PPD production and potency determination.Fil: Echeverría, Gustavo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Universidad Central del Ecuador; EcuadorFil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Proaño Pérez, Freddy. Universidad Central del Ecuador; EcuadorFil: Blasco, Francisco Barceló. Universidad de Las Américas.; EcuadorFil: de Waard, Jacobus H.. Universidad de Las Américas.; EcuadorNature Publishing Group2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/261734Echeverría, Gustavo; Zumárraga, Martín José; Proaño Pérez, Freddy; Blasco, Francisco Barceló; de Waard, Jacobus H.; Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis; Nature Publishing Group; Scientific Reports; 14; 1; 3-2024; 1-92045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-52089-1info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-52089-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:05Zoai:ri.conicet.gov.ar:11336/261734instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:06.116CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
title Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
spellingShingle Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
Echeverría, Gustavo
PPD
avian PPD
bovine PPD
IFN-y
title_short Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
title_full Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
title_fullStr Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
title_full_unstemmed Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
title_sort Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
dc.creator.none.fl_str_mv Echeverría, Gustavo
Zumárraga, Martín José
Proaño Pérez, Freddy
Blasco, Francisco Barceló
de Waard, Jacobus H.
author Echeverría, Gustavo
author_facet Echeverría, Gustavo
Zumárraga, Martín José
Proaño Pérez, Freddy
Blasco, Francisco Barceló
de Waard, Jacobus H.
author_role author
author2 Zumárraga, Martín José
Proaño Pérez, Freddy
Blasco, Francisco Barceló
de Waard, Jacobus H.
author2_role author
author
author
author
dc.subject.none.fl_str_mv PPD
avian PPD
bovine PPD
IFN-y
topic PPD
avian PPD
bovine PPD
IFN-y
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Although several brands of tuberculin purifed protein derivatives (PPDs) are available for diagnosing bovine tuberculosis (bTB), comparative studies to determine their diagnostic accuracy are infrequent. In Ecuador we compared two diferent PPD brands for bTB diagnosis using skin testing and measuring skin thickness increase. Additionally, we evaluated four PPD brands, including those used for skin testing, in the Bovine Tuberculosis Interferon Gamma Test (IFN-γ test) measuring IFN-γ induction in whole blood. The study included 17 naturally tuberculosis-infected PPD and IFN-γ test positive bovines. Both the feld and laboratory results showed signifcant diferences in classifying the 17 bovines as bTB positive or negative. We hypothesize that several factors, such as the genetic background of the cows, sensitization to environmental mycobacteria, M. bovis strains involved in the bTB infection, and the manufacturing procedures of the PPDs, could have infuenced the immune reaction toward the diferent tuberculin PPD brands. Our study emphasizes the necessity for comparative studies aimed at determining the diagnostic accuracy of PPD brands for bTB diagnosis as well as the development of standardized methods for PPD production and potency determination.
Fil: Echeverría, Gustavo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Universidad Central del Ecuador; Ecuador
Fil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Proaño Pérez, Freddy. Universidad Central del Ecuador; Ecuador
Fil: Blasco, Francisco Barceló. Universidad de Las Américas.; Ecuador
Fil: de Waard, Jacobus H.. Universidad de Las Américas.; Ecuador
description Although several brands of tuberculin purifed protein derivatives (PPDs) are available for diagnosing bovine tuberculosis (bTB), comparative studies to determine their diagnostic accuracy are infrequent. In Ecuador we compared two diferent PPD brands for bTB diagnosis using skin testing and measuring skin thickness increase. Additionally, we evaluated four PPD brands, including those used for skin testing, in the Bovine Tuberculosis Interferon Gamma Test (IFN-γ test) measuring IFN-γ induction in whole blood. The study included 17 naturally tuberculosis-infected PPD and IFN-γ test positive bovines. Both the feld and laboratory results showed signifcant diferences in classifying the 17 bovines as bTB positive or negative. We hypothesize that several factors, such as the genetic background of the cows, sensitization to environmental mycobacteria, M. bovis strains involved in the bTB infection, and the manufacturing procedures of the PPDs, could have infuenced the immune reaction toward the diferent tuberculin PPD brands. Our study emphasizes the necessity for comparative studies aimed at determining the diagnostic accuracy of PPD brands for bTB diagnosis as well as the development of standardized methods for PPD production and potency determination.
publishDate 2024
dc.date.none.fl_str_mv 2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/261734
Echeverría, Gustavo; Zumárraga, Martín José; Proaño Pérez, Freddy; Blasco, Francisco Barceló; de Waard, Jacobus H.; Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis; Nature Publishing Group; Scientific Reports; 14; 1; 3-2024; 1-9
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/261734
identifier_str_mv Echeverría, Gustavo; Zumárraga, Martín José; Proaño Pérez, Freddy; Blasco, Francisco Barceló; de Waard, Jacobus H.; Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis; Nature Publishing Group; Scientific Reports; 14; 1; 3-2024; 1-9
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-52089-1
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-52089-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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