Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy

Autores: Santa Cruz, Iara Sofía; Santa Cruz, Iara Sofía; Garabalino, Marcela Alejandra; Garabalino, Marcela Alejandra; Trivillin, Verónica Andrea; Trivillin, Verónica Andrea; Itoiz, María Elina; Itoiz, María Elina; Pozzi, Emiliano César Cayetano; Pozzi, Emiliano César Cayetano; Thorp, Silvia Inés; Thorp, Silvia Inés; Curotto, Paula; Curotto, Paula; Guidobono, Juan Santiago; Guidobono, Juan Santiago; Heber, Elisa Mercedes; Heber, Elisa Mercedes; Nigg, David W.; Nigg, David W.; Schwint, Amanda Elena; Schwint, Amanda Elena; Monti Hughes, Andrea; Monti Hughes, Andrea
Año de publicación: 2020
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Objective(s): The hamster carcinogenesis model recapitulates oral oncogenesis. Dimethylbenz[a]anthracene (DMBA) cancerization induces early severe mucositis, affecting animal's welfare and causing tissue loss and pouch shortening. “Short” pouches cannot be everted for local irradiation for boron neutron capture therapy (BNCT). Our aim was to optimize the DMBA classical cancerization protocol to avoid severe mucositis, without affecting tumor development. We evaluated BNCT in animals cancerized with this novel protocol. Materials and methods: We studied: Classical cancerization protocol (24 applications) and Classical with two interruptions (completed at the end of the cancerization protocol). BNCT mediated by boronophenylalanine (BPA) was performed in both groups. Results: The twice-interrupted group exhibited a significantly lower percentage of animals with severe mucositis versus the non-interrupted group (17% versus 71%) and a significantly higher incidence of long pouches (100% versus 53%). Tumor development and the histologic characteristics of tumor and precancerous tissue were not affected by the interruptions. For both groups, overall tumor response was more than 80%, with a similar incidence of BNCT-induced severe mucositis. Conclusion(s): The twice-interrupted protocol reduced severe mucositis during cancerization without affecting tumor development. This favored the animal's welfare and reduced the number of animals to be cancerized for our studies, without affecting BNCT response.
Objective(s): The hamster carcinogenesis model recapitulates oral oncogenesis. Dimethylbenz[a]anthracene (DMBA) cancerization induces early severe mucositis, affecting animal's welfare and causing tissue loss and pouch shortening. “Short” pouches cannot be everted for local irradiation for boron neutron capture therapy (BNCT). Our aim was to optimize the DMBA classical cancerization protocol to avoid severe mucositis, without affecting tumor development. We evaluated BNCT in animals cancerized with this novel protocol. Materials and methods: We studied: Classical cancerization protocol (24 applications) and Classical with two interruptions (completed at the end of the cancerization protocol). BNCT mediated by boronophenylalanine (BPA) was performed in both groups. Results: The twice-interrupted group exhibited a significantly lower percentage of animals with severe mucositis versus the non-interrupted group (17% versus 71%) and a significantly higher incidence of long pouches (100% versus 53%). Tumor development and the histologic characteristics of tumor and precancerous tissue were not affected by the interruptions. For both groups, overall tumor response was more than 80%, with a similar incidence of BNCT-induced severe mucositis. Conclusion(s): The twice-interrupted protocol reduced severe mucositis during cancerization without affecting tumor development. This favored the animal's welfare and reduced the number of animals to be cancerized for our studies, without affecting BNCT response.
Fil: Santa Cruz, Iara Sofía. Comisión Nacional de Energía Atómica; Argentina
Fil: Santa Cruz, Iara Sofía. Comisión Nacional de Energía Atómica; Argentina
Fil: Garabalino, Marcela Alejandra. Comisión Nacional de Energía Atómica; Argentina
Fil: Garabalino, Marcela Alejandra. Comisión Nacional de Energía Atómica; Argentina
Fil: Trivillin, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Trivillin, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Itoiz, María Elina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Itoiz, María Elina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; Argentina
Fil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; Argentina
Fil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; Argentina
Fil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; Argentina
Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; Argentina
Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; Argentina
Fil: Guidobono, Juan Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Guidobono, Juan Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Heber, Elisa Mercedes. Comisión Nacional de Energía Atómica; Argentina
Fil: Heber, Elisa Mercedes. Comisión Nacional de Energía Atómica; Argentina
Fil: Nigg, David W.. Idaho National Laboratory; Estados Unidos
Fil: Nigg, David W.. Idaho National Laboratory; Estados Unidos
Fil: Schwint, Amanda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Schwint, Amanda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Monti Hughes, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Monti Hughes, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Materia: ANIMAL WELFARE
ANIMAL WELFARE
BORON NEUTRON CAPTURE THERAPY
BORON NEUTRON CAPTURE THERAPY
CARCINOGENESIS
CARCINOGENESIS
HAMSTER CHEEK POUCH
HAMSTER CHEEK POUCH
MUCOSITIS
MUCOSITIS
ORAL CANCER
ORAL CANCER
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/168836

Acceder

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oai_identifier_str	oai:ri.conicet.gov.ar:11336/168836
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapyOptimization of the classical oral cancerization protocol in hamster to study oral cancer therapySanta Cruz, Iara SofíaSanta Cruz, Iara SofíaGarabalino, Marcela AlejandraGarabalino, Marcela AlejandraTrivillin, Verónica AndreaTrivillin, Verónica AndreaItoiz, María ElinaItoiz, María ElinaPozzi, Emiliano César CayetanoPozzi, Emiliano César CayetanoThorp, Silvia InésThorp, Silvia InésCurotto, PaulaCurotto, PaulaGuidobono, Juan SantiagoGuidobono, Juan SantiagoHeber, Elisa MercedesHeber, Elisa MercedesNigg, David W.Nigg, David W.Schwint, Amanda ElenaSchwint, Amanda ElenaMonti Hughes, AndreaMonti Hughes, AndreaANIMAL WELFAREANIMAL WELFAREBORON NEUTRON CAPTURE THERAPYBORON NEUTRON CAPTURE THERAPYCARCINOGENESISCARCINOGENESISHAMSTER CHEEK POUCHHAMSTER CHEEK POUCHMUCOSITISMUCOSITISORAL CANCERORAL CANCERhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3Objective(s): The hamster carcinogenesis model recapitulates oral oncogenesis. Dimethylbenz[a]anthracene (DMBA) cancerization induces early severe mucositis, affecting animal's welfare and causing tissue loss and pouch shortening. “Short” pouches cannot be everted for local irradiation for boron neutron capture therapy (BNCT). Our aim was to optimize the DMBA classical cancerization protocol to avoid severe mucositis, without affecting tumor development. We evaluated BNCT in animals cancerized with this novel protocol. Materials and methods: We studied: Classical cancerization protocol (24 applications) and Classical with two interruptions (completed at the end of the cancerization protocol). BNCT mediated by boronophenylalanine (BPA) was performed in both groups. Results: The twice-interrupted group exhibited a significantly lower percentage of animals with severe mucositis versus the non-interrupted group (17% versus 71%) and a significantly higher incidence of long pouches (100% versus 53%). Tumor development and the histologic characteristics of tumor and precancerous tissue were not affected by the interruptions. For both groups, overall tumor response was more than 80%, with a similar incidence of BNCT-induced severe mucositis. Conclusion(s): The twice-interrupted protocol reduced severe mucositis during cancerization without affecting tumor development. This favored the animal's welfare and reduced the number of animals to be cancerized for our studies, without affecting BNCT response.Objective(s): The hamster carcinogenesis model recapitulates oral oncogenesis. Dimethylbenz[a]anthracene (DMBA) cancerization induces early severe mucositis, affecting animal's welfare and causing tissue loss and pouch shortening. “Short” pouches cannot be everted for local irradiation for boron neutron capture therapy (BNCT). Our aim was to optimize the DMBA classical cancerization protocol to avoid severe mucositis, without affecting tumor development. We evaluated BNCT in animals cancerized with this novel protocol. Materials and methods: We studied: Classical cancerization protocol (24 applications) and Classical with two interruptions (completed at the end of the cancerization protocol). BNCT mediated by boronophenylalanine (BPA) was performed in both groups. Results: The twice-interrupted group exhibited a significantly lower percentage of animals with severe mucositis versus the non-interrupted group (17% versus 71%) and a significantly higher incidence of long pouches (100% versus 53%). Tumor development and the histologic characteristics of tumor and precancerous tissue were not affected by the interruptions. For both groups, overall tumor response was more than 80%, with a similar incidence of BNCT-induced severe mucositis. Conclusion(s): The twice-interrupted protocol reduced severe mucositis during cancerization without affecting tumor development. This favored the animal's welfare and reduced the number of animals to be cancerized for our studies, without affecting BNCT response.Fil: Santa Cruz, Iara Sofía. Comisión Nacional de Energía Atómica; ArgentinaFil: Santa Cruz, Iara Sofía. Comisión Nacional de Energía Atómica; ArgentinaFil: Garabalino, Marcela Alejandra. Comisión Nacional de Energía Atómica; ArgentinaFil: Garabalino, Marcela Alejandra. Comisión Nacional de Energía Atómica; ArgentinaFil: Trivillin, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Trivillin, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Itoiz, María Elina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Itoiz, María Elina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; ArgentinaFil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; ArgentinaFil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; ArgentinaFil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; ArgentinaFil: Curotto, Paula. Comisión Nacional de Energía Atómica; ArgentinaFil: Curotto, Paula. Comisión Nacional de Energía Atómica; ArgentinaFil: Guidobono, Juan Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Guidobono, Juan Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Heber, Elisa Mercedes. Comisión Nacional de Energía Atómica; ArgentinaFil: Heber, Elisa Mercedes. Comisión Nacional de Energía Atómica; ArgentinaFil: Nigg, David W.. Idaho National Laboratory; Estados UnidosFil: Nigg, David W.. Idaho National Laboratory; Estados UnidosFil: Schwint, Amanda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Schwint, Amanda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Monti Hughes, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Monti Hughes, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaWiley Blackwell Publishing, IncWiley Blackwell Publishing, Inc2020-092020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/168836Santa Cruz, Iara Sofía; Garabalino, Marcela Alejandra; Trivillin, Verónica Andrea; Itoiz, María Elina; Pozzi, Emiliano César Cayetano; et al.; Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy; Wiley Blackwell Publishing, Inc; Oral Diseases; 26; 6; 9-2020; 1-27Santa Cruz, Iara Sofía; Garabalino, Marcela Alejandra; Trivillin, Verónica Andrea; Itoiz, María Elina; Pozzi, Emiliano César Cayetano; et al.; Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy; Wiley Blackwell Publishing, Inc; Oral Diseases; 26; 6; 9-2020; 1-271354-523X1354-523X1601-08251601-0825CONICET DigitalCONICETengenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/odi.13358info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/odi.13358info:eu-repo/semantics/altIdentifier/doi/10.1111/odi.13358info:eu-repo/semantics/altIdentifier/doi/10.1111/odi.13358info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:23:52Zoai:ri.conicet.gov.ar:11336/168836instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:23:52.757CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy
title	Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy
spellingShingle	Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy Santa Cruz, Iara Sofía ANIMAL WELFARE ANIMAL WELFARE BORON NEUTRON CAPTURE THERAPY BORON NEUTRON CAPTURE THERAPY CARCINOGENESIS CARCINOGENESIS HAMSTER CHEEK POUCH HAMSTER CHEEK POUCH MUCOSITIS MUCOSITIS ORAL CANCER ORAL CANCER
title_short	Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy
title_full	Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy
title_fullStr	Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy
title_full_unstemmed	Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy
title_sort	Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy
dc.creator.none.fl_str_mv	Santa Cruz, Iara Sofía Santa Cruz, Iara Sofía Garabalino, Marcela Alejandra Garabalino, Marcela Alejandra Trivillin, Verónica Andrea Trivillin, Verónica Andrea Itoiz, María Elina Itoiz, María Elina Pozzi, Emiliano César Cayetano Pozzi, Emiliano César Cayetano Thorp, Silvia Inés Thorp, Silvia Inés Curotto, Paula Curotto, Paula Guidobono, Juan Santiago Guidobono, Juan Santiago Heber, Elisa Mercedes Heber, Elisa Mercedes Nigg, David W. Nigg, David W. Schwint, Amanda Elena Schwint, Amanda Elena Monti Hughes, Andrea Monti Hughes, Andrea
author	Santa Cruz, Iara Sofía
author_facet	Santa Cruz, Iara Sofía Garabalino, Marcela Alejandra Trivillin, Verónica Andrea Itoiz, María Elina Pozzi, Emiliano César Cayetano Thorp, Silvia Inés Curotto, Paula Guidobono, Juan Santiago Heber, Elisa Mercedes Nigg, David W. Schwint, Amanda Elena Monti Hughes, Andrea
author_role	author
author2	Garabalino, Marcela Alejandra Trivillin, Verónica Andrea Itoiz, María Elina Pozzi, Emiliano César Cayetano Thorp, Silvia Inés Curotto, Paula Guidobono, Juan Santiago Heber, Elisa Mercedes Nigg, David W. Schwint, Amanda Elena Monti Hughes, Andrea
author2_role	author author author author author author author author author author author
dc.subject.none.fl_str_mv	ANIMAL WELFARE ANIMAL WELFARE BORON NEUTRON CAPTURE THERAPY BORON NEUTRON CAPTURE THERAPY CARCINOGENESIS CARCINOGENESIS HAMSTER CHEEK POUCH HAMSTER CHEEK POUCH MUCOSITIS MUCOSITIS ORAL CANCER ORAL CANCER
topic	ANIMAL WELFARE ANIMAL WELFARE BORON NEUTRON CAPTURE THERAPY BORON NEUTRON CAPTURE THERAPY CARCINOGENESIS CARCINOGENESIS HAMSTER CHEEK POUCH HAMSTER CHEEK POUCH MUCOSITIS MUCOSITIS ORAL CANCER ORAL CANCER
purl_subject.fl_str_mv	https://purl.org/becyt/ford/3.5 https://purl.org/becyt/ford/3.5 https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv	Objective(s): The hamster carcinogenesis model recapitulates oral oncogenesis. Dimethylbenz[a]anthracene (DMBA) cancerization induces early severe mucositis, affecting animal's welfare and causing tissue loss and pouch shortening. “Short” pouches cannot be everted for local irradiation for boron neutron capture therapy (BNCT). Our aim was to optimize the DMBA classical cancerization protocol to avoid severe mucositis, without affecting tumor development. We evaluated BNCT in animals cancerized with this novel protocol. Materials and methods: We studied: Classical cancerization protocol (24 applications) and Classical with two interruptions (completed at the end of the cancerization protocol). BNCT mediated by boronophenylalanine (BPA) was performed in both groups. Results: The twice-interrupted group exhibited a significantly lower percentage of animals with severe mucositis versus the non-interrupted group (17% versus 71%) and a significantly higher incidence of long pouches (100% versus 53%). Tumor development and the histologic characteristics of tumor and precancerous tissue were not affected by the interruptions. For both groups, overall tumor response was more than 80%, with a similar incidence of BNCT-induced severe mucositis. Conclusion(s): The twice-interrupted protocol reduced severe mucositis during cancerization without affecting tumor development. This favored the animal's welfare and reduced the number of animals to be cancerized for our studies, without affecting BNCT response. Objective(s): The hamster carcinogenesis model recapitulates oral oncogenesis. Dimethylbenz[a]anthracene (DMBA) cancerization induces early severe mucositis, affecting animal's welfare and causing tissue loss and pouch shortening. “Short” pouches cannot be everted for local irradiation for boron neutron capture therapy (BNCT). Our aim was to optimize the DMBA classical cancerization protocol to avoid severe mucositis, without affecting tumor development. We evaluated BNCT in animals cancerized with this novel protocol. Materials and methods: We studied: Classical cancerization protocol (24 applications) and Classical with two interruptions (completed at the end of the cancerization protocol). BNCT mediated by boronophenylalanine (BPA) was performed in both groups. Results: The twice-interrupted group exhibited a significantly lower percentage of animals with severe mucositis versus the non-interrupted group (17% versus 71%) and a significantly higher incidence of long pouches (100% versus 53%). Tumor development and the histologic characteristics of tumor and precancerous tissue were not affected by the interruptions. For both groups, overall tumor response was more than 80%, with a similar incidence of BNCT-induced severe mucositis. Conclusion(s): The twice-interrupted protocol reduced severe mucositis during cancerization without affecting tumor development. This favored the animal's welfare and reduced the number of animals to be cancerized for our studies, without affecting BNCT response. Fil: Santa Cruz, Iara Sofía. Comisión Nacional de Energía Atómica; Argentina Fil: Santa Cruz, Iara Sofía. Comisión Nacional de Energía Atómica; Argentina Fil: Garabalino, Marcela Alejandra. Comisión Nacional de Energía Atómica; Argentina Fil: Garabalino, Marcela Alejandra. Comisión Nacional de Energía Atómica; Argentina Fil: Trivillin, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina Fil: Trivillin, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina Fil: Itoiz, María Elina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina Fil: Itoiz, María Elina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina Fil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; Argentina Fil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; Argentina Fil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; Argentina Fil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; Argentina Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; Argentina Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; Argentina Fil: Guidobono, Juan Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Guidobono, Juan Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Heber, Elisa Mercedes. Comisión Nacional de Energía Atómica; Argentina Fil: Heber, Elisa Mercedes. Comisión Nacional de Energía Atómica; Argentina Fil: Nigg, David W.. Idaho National Laboratory; Estados Unidos Fil: Nigg, David W.. Idaho National Laboratory; Estados Unidos Fil: Schwint, Amanda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina Fil: Schwint, Amanda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina Fil: Monti Hughes, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina Fil: Monti Hughes, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
description	Objective(s): The hamster carcinogenesis model recapitulates oral oncogenesis. Dimethylbenz[a]anthracene (DMBA) cancerization induces early severe mucositis, affecting animal's welfare and causing tissue loss and pouch shortening. “Short” pouches cannot be everted for local irradiation for boron neutron capture therapy (BNCT). Our aim was to optimize the DMBA classical cancerization protocol to avoid severe mucositis, without affecting tumor development. We evaluated BNCT in animals cancerized with this novel protocol. Materials and methods: We studied: Classical cancerization protocol (24 applications) and Classical with two interruptions (completed at the end of the cancerization protocol). BNCT mediated by boronophenylalanine (BPA) was performed in both groups. Results: The twice-interrupted group exhibited a significantly lower percentage of animals with severe mucositis versus the non-interrupted group (17% versus 71%) and a significantly higher incidence of long pouches (100% versus 53%). Tumor development and the histologic characteristics of tumor and precancerous tissue were not affected by the interruptions. For both groups, overall tumor response was more than 80%, with a similar incidence of BNCT-induced severe mucositis. Conclusion(s): The twice-interrupted protocol reduced severe mucositis during cancerization without affecting tumor development. This favored the animal's welfare and reduced the number of animals to be cancerized for our studies, without affecting BNCT response.
publishDate	2020
dc.date.none.fl_str_mv	2020-09 2020-09
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/168836 Santa Cruz, Iara Sofía; Garabalino, Marcela Alejandra; Trivillin, Verónica Andrea; Itoiz, María Elina; Pozzi, Emiliano César Cayetano; et al.; Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy; Wiley Blackwell Publishing, Inc; Oral Diseases; 26; 6; 9-2020; 1-27 Santa Cruz, Iara Sofía; Garabalino, Marcela Alejandra; Trivillin, Verónica Andrea; Itoiz, María Elina; Pozzi, Emiliano César Cayetano; et al.; Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy; Wiley Blackwell Publishing, Inc; Oral Diseases; 26; 6; 9-2020; 1-27 1354-523X 1354-523X 1601-0825 1601-0825 CONICET Digital CONICET
url	http://hdl.handle.net/11336/168836
identifier_str_mv	Santa Cruz, Iara Sofía; Garabalino, Marcela Alejandra; Trivillin, Verónica Andrea; Itoiz, María Elina; Pozzi, Emiliano César Cayetano; et al.; Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy; Wiley Blackwell Publishing, Inc; Oral Diseases; 26; 6; 9-2020; 1-27 1354-523X 1601-0825 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/odi.13358 info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/odi.13358 info:eu-repo/semantics/altIdentifier/doi/10.1111/odi.13358 info:eu-repo/semantics/altIdentifier/doi/10.1111/odi.13358
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Wiley Blackwell Publishing, Inc Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv	Wiley Blackwell Publishing, Inc Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy

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