pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogels

Autores
Galaburri, Gonzalo; Peralta Ramos, María Luz; Lazaro Martinez, Juan Manuel; Fernández de Luis, Roberto; Arriortua, María Isabel; Villanueva, María Emilia; Copello, Guillermo Javier
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The oxidation of keratin hydrogels was optimized aiming to obtain keratose hydrogels while maintaining the original 3D structure and pH-responsive behaviour. In addition, we present a comparative study of their dual-stimuli responsive behaviour regarding the pH-responsiveness and their previously non-described ion selective responsiveness. Keratose and keratin hydrogels showed similar swellings when contracted at low pH, and the former showed steeper expansion and higher swelling than the latter at high pH. In addition, both hydrogels showed selective responsiveness toward Ca2+ ions when expanded, and toward Cu2+ ions when contracted. IR, Raman, ss-NMR, HRMAS NMR, SAXS, and thermal characterization demonstrated that oxidation does not interfere with the stimuli-responsive mechanisms related to protein conformation. The higher swelling of keratose hydrogels would be originated in the increase of the mobility of the chains after oxidation coupled to the hydration of sulfonic acid groups.
Fil: Galaburri, Gonzalo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina
Fil: Peralta Ramos, María Luz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina
Fil: Lazaro Martinez, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Fernández de Luis, Roberto. No especifíca;
Fil: Arriortua, María Isabel. Universidad del País Vasco; España
Fil: Villanueva, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Copello, Guillermo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Materia
HYDROGEL
KERATIN
KERATOSE
SMART MATERIAL
STIMULI-RESPONSIVE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/120283
Acceder
id CONICETDig_ba0d5855124c4f0a1ac0563505084921
oai_identifier_str oai:ri.conicet.gov.ar:11336/120283
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogelsGalaburri, GonzaloPeralta Ramos, María LuzLazaro Martinez, Juan ManuelFernández de Luis, RobertoArriortua, María IsabelVillanueva, María EmiliaCopello, Guillermo JavierHYDROGELKERATINKERATOSESMART MATERIALSTIMULI-RESPONSIVEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The oxidation of keratin hydrogels was optimized aiming to obtain keratose hydrogels while maintaining the original 3D structure and pH-responsive behaviour. In addition, we present a comparative study of their dual-stimuli responsive behaviour regarding the pH-responsiveness and their previously non-described ion selective responsiveness. Keratose and keratin hydrogels showed similar swellings when contracted at low pH, and the former showed steeper expansion and higher swelling than the latter at high pH. In addition, both hydrogels showed selective responsiveness toward Ca2+ ions when expanded, and toward Cu2+ ions when contracted. IR, Raman, ss-NMR, HRMAS NMR, SAXS, and thermal characterization demonstrated that oxidation does not interfere with the stimuli-responsive mechanisms related to protein conformation. The higher swelling of keratose hydrogels would be originated in the increase of the mobility of the chains after oxidation coupled to the hydration of sulfonic acid groups.Fil: Galaburri, Gonzalo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; ArgentinaFil: Peralta Ramos, María Luz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; ArgentinaFil: Lazaro Martinez, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Fernández de Luis, Roberto. No especifíca;Fil: Arriortua, María Isabel. Universidad del País Vasco; EspañaFil: Villanueva, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Copello, Guillermo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaPergamon-Elsevier Science Ltd2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/120283Galaburri, Gonzalo; Peralta Ramos, María Luz; Lazaro Martinez, Juan Manuel; Fernández de Luis, Roberto; Arriortua, María Isabel; et al.; pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogels; Pergamon-Elsevier Science Ltd; European Polymer Journal; 118; 9-2019; 1-90014-3057CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0014305719303623info:eu-repo/semantics/altIdentifier/doi/10.1016/j.eurpolymj.2019.05.043info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:16:34Zoai:ri.conicet.gov.ar:11336/120283instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:16:34.292CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogels
title pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogels
spellingShingle pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogels
Galaburri, Gonzalo
HYDROGEL
KERATIN
KERATOSE
SMART MATERIAL
STIMULI-RESPONSIVE
title_short pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogels
title_full pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogels
title_fullStr pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogels
title_full_unstemmed pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogels
title_sort pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogels
dc.creator.none.fl_str_mv Galaburri, Gonzalo
Peralta Ramos, María Luz
Lazaro Martinez, Juan Manuel
Fernández de Luis, Roberto
Arriortua, María Isabel
Villanueva, María Emilia
Copello, Guillermo Javier
author Galaburri, Gonzalo
author_facet Galaburri, Gonzalo
Peralta Ramos, María Luz
Lazaro Martinez, Juan Manuel
Fernández de Luis, Roberto
Arriortua, María Isabel
Villanueva, María Emilia
Copello, Guillermo Javier
author_role author
author2 Peralta Ramos, María Luz
Lazaro Martinez, Juan Manuel
Fernández de Luis, Roberto
Arriortua, María Isabel
Villanueva, María Emilia
Copello, Guillermo Javier
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv HYDROGEL
KERATIN
KERATOSE
SMART MATERIAL
STIMULI-RESPONSIVE
topic HYDROGEL
KERATIN
KERATOSE
SMART MATERIAL
STIMULI-RESPONSIVE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The oxidation of keratin hydrogels was optimized aiming to obtain keratose hydrogels while maintaining the original 3D structure and pH-responsive behaviour. In addition, we present a comparative study of their dual-stimuli responsive behaviour regarding the pH-responsiveness and their previously non-described ion selective responsiveness. Keratose and keratin hydrogels showed similar swellings when contracted at low pH, and the former showed steeper expansion and higher swelling than the latter at high pH. In addition, both hydrogels showed selective responsiveness toward Ca2+ ions when expanded, and toward Cu2+ ions when contracted. IR, Raman, ss-NMR, HRMAS NMR, SAXS, and thermal characterization demonstrated that oxidation does not interfere with the stimuli-responsive mechanisms related to protein conformation. The higher swelling of keratose hydrogels would be originated in the increase of the mobility of the chains after oxidation coupled to the hydration of sulfonic acid groups.
Fil: Galaburri, Gonzalo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina
Fil: Peralta Ramos, María Luz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina
Fil: Lazaro Martinez, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Fernández de Luis, Roberto. No especifíca;
Fil: Arriortua, María Isabel. Universidad del País Vasco; España
Fil: Villanueva, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Copello, Guillermo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
description The oxidation of keratin hydrogels was optimized aiming to obtain keratose hydrogels while maintaining the original 3D structure and pH-responsive behaviour. In addition, we present a comparative study of their dual-stimuli responsive behaviour regarding the pH-responsiveness and their previously non-described ion selective responsiveness. Keratose and keratin hydrogels showed similar swellings when contracted at low pH, and the former showed steeper expansion and higher swelling than the latter at high pH. In addition, both hydrogels showed selective responsiveness toward Ca2+ ions when expanded, and toward Cu2+ ions when contracted. IR, Raman, ss-NMR, HRMAS NMR, SAXS, and thermal characterization demonstrated that oxidation does not interfere with the stimuli-responsive mechanisms related to protein conformation. The higher swelling of keratose hydrogels would be originated in the increase of the mobility of the chains after oxidation coupled to the hydration of sulfonic acid groups.
publishDate 2019
dc.date.none.fl_str_mv 2019-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/120283
Galaburri, Gonzalo; Peralta Ramos, María Luz; Lazaro Martinez, Juan Manuel; Fernández de Luis, Roberto; Arriortua, María Isabel; et al.; pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogels; Pergamon-Elsevier Science Ltd; European Polymer Journal; 118; 9-2019; 1-9
0014-3057
CONICET Digital
CONICET
url http://hdl.handle.net/11336/120283
identifier_str_mv Galaburri, Gonzalo; Peralta Ramos, María Luz; Lazaro Martinez, Juan Manuel; Fernández de Luis, Roberto; Arriortua, María Isabel; et al.; pH and ion-selective swelling behaviour of keratin and keratose 3D hydrogels; Pergamon-Elsevier Science Ltd; European Polymer Journal; 118; 9-2019; 1-9
0014-3057
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0014305719303623
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.eurpolymj.2019.05.043
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781616292102144
score 12.982451

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