Cell-free biosensors for rapid detection of water contaminants

Autores
Jung, Jaeyoung K.; Alam, Khalid K.; Verosloff, Matthew S.; Capdevila, Daiana Andrea; Desmau, Morgane; Clauer, Phillip R.; Lee, Jeong Wook; Nguyen, Peter Q.; Pastén, Pablo A.; Matiasek, Sandrine J.; Gaillard, Jean-François; Giedroc, David Peter; Collins, James J.; Lucks, Julius B.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lack of access to safe drinking water is a global problem, and methods to reliably and easily detect contaminants could be transformative. We report the development of a cell-free in vitro transcription system that uses RNA Output Sensors Activated by Ligand Induction (ROSALIND) to detect contaminants in water. A combination of highly processive RNA polymerases, allosteric protein transcription factors and synthetic DNA transcription templates regulates the synthesis of a fluorescence-activating RNA aptamer. The presence of a target contaminant induces the transcription of the aptamer, and a fluorescent signal is produced. We apply ROSALIND to detect a range of water contaminants, including antibiotics, small molecules and metals. We also show that adding RNA circuitry can invert responses, reduce crosstalk and improve sensitivity without protein engineering. The ROSALIND system can be freeze-dried for easy storage and distribution, and we apply it in the field to test municipal water supplies, demonstrating its potential use for monitoring water quality.
Fil: Jung, Jaeyoung K.. Northwestern University; Estados Unidos
Fil: Alam, Khalid K.. Northwestern University; Estados Unidos
Fil: Verosloff, Matthew S.. Northwestern University; Estados Unidos
Fil: Capdevila, Daiana Andrea. Indiana University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Desmau, Morgane. Northwestern University; Estados Unidos
Fil: Clauer, Phillip R.. Massachusetts Institute of Technology; Estados Unidos
Fil: Lee, Jeong Wook. Pohang University of Science and Technology; Corea del Sur
Fil: Nguyen, Peter Q.. Harvard University; Estados Unidos
Fil: Pastén, Pablo A.. Pontificia Universidad Católica de Chile; Chile
Fil: Matiasek, Sandrine J.. California State University; Estados Unidos
Fil: Gaillard, Jean-François. Northwestern University; Estados Unidos
Fil: Giedroc, David Peter. Indiana University; Estados Unidos
Fil: Collins, James J.. Massachusetts Institute of Technology; Estados Unidos
Fil: Lucks, Julius B.. Northwestern University; Estados Unidos
Materia
RNA Output Sensors Activated by Ligand Induction (ROSALIND)
cell-free in vitro transcription
water contaminants
metal ions
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/139072
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/139072
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cell-free biosensors for rapid detection of water contaminantsJung, Jaeyoung K.Alam, Khalid K.Verosloff, Matthew S.Capdevila, Daiana AndreaDesmau, MorganeClauer, Phillip R.Lee, Jeong WookNguyen, Peter Q.Pastén, Pablo A.Matiasek, Sandrine J.Gaillard, Jean-FrançoisGiedroc, David PeterCollins, James J.Lucks, Julius B.RNA Output Sensors Activated by Ligand Induction (ROSALIND)cell-free in vitro transcriptionwater contaminantsmetal ionshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lack of access to safe drinking water is a global problem, and methods to reliably and easily detect contaminants could be transformative. We report the development of a cell-free in vitro transcription system that uses RNA Output Sensors Activated by Ligand Induction (ROSALIND) to detect contaminants in water. A combination of highly processive RNA polymerases, allosteric protein transcription factors and synthetic DNA transcription templates regulates the synthesis of a fluorescence-activating RNA aptamer. The presence of a target contaminant induces the transcription of the aptamer, and a fluorescent signal is produced. We apply ROSALIND to detect a range of water contaminants, including antibiotics, small molecules and metals. We also show that adding RNA circuitry can invert responses, reduce crosstalk and improve sensitivity without protein engineering. The ROSALIND system can be freeze-dried for easy storage and distribution, and we apply it in the field to test municipal water supplies, demonstrating its potential use for monitoring water quality.Fil: Jung, Jaeyoung K.. Northwestern University; Estados UnidosFil: Alam, Khalid K.. Northwestern University; Estados UnidosFil: Verosloff, Matthew S.. Northwestern University; Estados UnidosFil: Capdevila, Daiana Andrea. Indiana University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Desmau, Morgane. Northwestern University; Estados UnidosFil: Clauer, Phillip R.. Massachusetts Institute of Technology; Estados UnidosFil: Lee, Jeong Wook. Pohang University of Science and Technology; Corea del SurFil: Nguyen, Peter Q.. Harvard University; Estados UnidosFil: Pastén, Pablo A.. Pontificia Universidad Católica de Chile; ChileFil: Matiasek, Sandrine J.. California State University; Estados UnidosFil: Gaillard, Jean-François. Northwestern University; Estados UnidosFil: Giedroc, David Peter. Indiana University; Estados UnidosFil: Collins, James J.. Massachusetts Institute of Technology; Estados UnidosFil: Lucks, Julius B.. Northwestern University; Estados UnidosNature Publishing Group2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139072Jung, Jaeyoung K.; Alam, Khalid K.; Verosloff, Matthew S.; Capdevila, Daiana Andrea; Desmau, Morgane; et al.; Cell-free biosensors for rapid detection of water contaminants; Nature Publishing Group; Nature Biotechnology; 38; 12; 7-2020; 1451-14591087-01561546-1696CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41587-020-0571-7info:eu-repo/semantics/altIdentifier/doi/10.1038/s41587-020-0571-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:09Zoai:ri.conicet.gov.ar:11336/139072instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:10.214CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cell-free biosensors for rapid detection of water contaminants
title Cell-free biosensors for rapid detection of water contaminants
spellingShingle Cell-free biosensors for rapid detection of water contaminants
Jung, Jaeyoung K.
RNA Output Sensors Activated by Ligand Induction (ROSALIND)
cell-free in vitro transcription
water contaminants
metal ions
title_short Cell-free biosensors for rapid detection of water contaminants
title_full Cell-free biosensors for rapid detection of water contaminants
title_fullStr Cell-free biosensors for rapid detection of water contaminants
title_full_unstemmed Cell-free biosensors for rapid detection of water contaminants
title_sort Cell-free biosensors for rapid detection of water contaminants
dc.creator.none.fl_str_mv Jung, Jaeyoung K.
Alam, Khalid K.
Verosloff, Matthew S.
Capdevila, Daiana Andrea
Desmau, Morgane
Clauer, Phillip R.
Lee, Jeong Wook
Nguyen, Peter Q.
Pastén, Pablo A.
Matiasek, Sandrine J.
Gaillard, Jean-François
Giedroc, David Peter
Collins, James J.
Lucks, Julius B.
author Jung, Jaeyoung K.
author_facet Jung, Jaeyoung K.
Alam, Khalid K.
Verosloff, Matthew S.
Capdevila, Daiana Andrea
Desmau, Morgane
Clauer, Phillip R.
Lee, Jeong Wook
Nguyen, Peter Q.
Pastén, Pablo A.
Matiasek, Sandrine J.
Gaillard, Jean-François
Giedroc, David Peter
Collins, James J.
Lucks, Julius B.
author_role author
author2 Alam, Khalid K.
Verosloff, Matthew S.
Capdevila, Daiana Andrea
Desmau, Morgane
Clauer, Phillip R.
Lee, Jeong Wook
Nguyen, Peter Q.
Pastén, Pablo A.
Matiasek, Sandrine J.
Gaillard, Jean-François
Giedroc, David Peter
Collins, James J.
Lucks, Julius B.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv RNA Output Sensors Activated by Ligand Induction (ROSALIND)
cell-free in vitro transcription
water contaminants
metal ions
topic RNA Output Sensors Activated by Ligand Induction (ROSALIND)
cell-free in vitro transcription
water contaminants
metal ions
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lack of access to safe drinking water is a global problem, and methods to reliably and easily detect contaminants could be transformative. We report the development of a cell-free in vitro transcription system that uses RNA Output Sensors Activated by Ligand Induction (ROSALIND) to detect contaminants in water. A combination of highly processive RNA polymerases, allosteric protein transcription factors and synthetic DNA transcription templates regulates the synthesis of a fluorescence-activating RNA aptamer. The presence of a target contaminant induces the transcription of the aptamer, and a fluorescent signal is produced. We apply ROSALIND to detect a range of water contaminants, including antibiotics, small molecules and metals. We also show that adding RNA circuitry can invert responses, reduce crosstalk and improve sensitivity without protein engineering. The ROSALIND system can be freeze-dried for easy storage and distribution, and we apply it in the field to test municipal water supplies, demonstrating its potential use for monitoring water quality.
Fil: Jung, Jaeyoung K.. Northwestern University; Estados Unidos
Fil: Alam, Khalid K.. Northwestern University; Estados Unidos
Fil: Verosloff, Matthew S.. Northwestern University; Estados Unidos
Fil: Capdevila, Daiana Andrea. Indiana University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Desmau, Morgane. Northwestern University; Estados Unidos
Fil: Clauer, Phillip R.. Massachusetts Institute of Technology; Estados Unidos
Fil: Lee, Jeong Wook. Pohang University of Science and Technology; Corea del Sur
Fil: Nguyen, Peter Q.. Harvard University; Estados Unidos
Fil: Pastén, Pablo A.. Pontificia Universidad Católica de Chile; Chile
Fil: Matiasek, Sandrine J.. California State University; Estados Unidos
Fil: Gaillard, Jean-François. Northwestern University; Estados Unidos
Fil: Giedroc, David Peter. Indiana University; Estados Unidos
Fil: Collins, James J.. Massachusetts Institute of Technology; Estados Unidos
Fil: Lucks, Julius B.. Northwestern University; Estados Unidos
description Lack of access to safe drinking water is a global problem, and methods to reliably and easily detect contaminants could be transformative. We report the development of a cell-free in vitro transcription system that uses RNA Output Sensors Activated by Ligand Induction (ROSALIND) to detect contaminants in water. A combination of highly processive RNA polymerases, allosteric protein transcription factors and synthetic DNA transcription templates regulates the synthesis of a fluorescence-activating RNA aptamer. The presence of a target contaminant induces the transcription of the aptamer, and a fluorescent signal is produced. We apply ROSALIND to detect a range of water contaminants, including antibiotics, small molecules and metals. We also show that adding RNA circuitry can invert responses, reduce crosstalk and improve sensitivity without protein engineering. The ROSALIND system can be freeze-dried for easy storage and distribution, and we apply it in the field to test municipal water supplies, demonstrating its potential use for monitoring water quality.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/139072
Jung, Jaeyoung K.; Alam, Khalid K.; Verosloff, Matthew S.; Capdevila, Daiana Andrea; Desmau, Morgane; et al.; Cell-free biosensors for rapid detection of water contaminants; Nature Publishing Group; Nature Biotechnology; 38; 12; 7-2020; 1451-1459
1087-0156
1546-1696
CONICET Digital
CONICET
url http://hdl.handle.net/11336/139072
identifier_str_mv Jung, Jaeyoung K.; Alam, Khalid K.; Verosloff, Matthew S.; Capdevila, Daiana Andrea; Desmau, Morgane; et al.; Cell-free biosensors for rapid detection of water contaminants; Nature Publishing Group; Nature Biotechnology; 38; 12; 7-2020; 1451-1459
1087-0156
1546-1696
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41587-020-0571-7
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41587-020-0571-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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