Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated...

Autores: Martínez Galera, M.; Gil García, Magdalena; Culzoni, Maria Julia; Goicoechea, Hector Casimiro
Año de publicación: 2010
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: An analytical method for the simultaneous determination of nine -blockers (sotalol atenolol, nadolol, pindolol, metoprolol, timolol, bisoprolol, propanolol and betaxolol) and two analgesics (paracetamol and phenazone) in river water by liquid chromatography and diode array detection is reported. The method involves a modified precolumn switching methodology replacing the small precolumn with a short C18 liquid chromatography column (50mm×4.6mm,5mparticle size), thus allowing the preconcent ration of large water sample volumes whereas interferences eluting at the first of the chromatogram were discarded to waste. This approach allowed to preconcentrate 30mL river water samples, modified with 0.4% MeOH, achieving univariate method detection and determination limits ranged between 0.03 and 0.16gL−1 and between 0.2 and 0.5gL−1, respectively, with precision values lower than 9.4% for spiking levels at the quantitation limits of each analyte and lower than 4.0%, except bisoprolol (8.3%), for higherspiking levels (1.0gL−1 of all analytes). Matrix background was reduced in three way data by a baseline correction following the Eilers methodology, whereas multivariate curve resolution and alternating least squares in combination with the standard addition calibration method, applied to these data, coped with overlapping peak, systematic (additive) and proportional (matrix effect) errors. The method was successfully applied for the determination of the target pharmaceuticals in river water from three places in a river stream with acceptable recoveries and precision values, taking into account the complexity of the analytical problem. The joint statistical test for the slope and the intercept of the linear regression between the nominal concentration values versus those predicted, showed that the region computed contained the theoretically expected values (0) for the intercept and (1) for the slope (at a confidence level of 95%), which indicates the absence of both constant and proportional errors in the predicted concentrations.
Fil: Martínez Galera, M.. Universidad de Almería.; España
Fil: Gil García, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones en Humanidades y Ciencias Sociales. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación. Instituto de Investigaciones en Humanidades y Ciencias Sociales; Argentina
Fil: Culzoni, Maria Julia. Universidad Nacional del Litoral; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina
Materia: MCR
ALS
CHROMATOGRAPHY
EMERGENT CONTAMINANT
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/103399

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spelling	Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated approach to green analytical methodologiesMartínez Galera, M.Gil García, MagdalenaCulzoni, Maria JuliaGoicoechea, Hector CasimiroMCRALSCHROMATOGRAPHYEMERGENT CONTAMINANThttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An analytical method for the simultaneous determination of nine -blockers (sotalol atenolol, nadolol, pindolol, metoprolol, timolol, bisoprolol, propanolol and betaxolol) and two analgesics (paracetamol and phenazone) in river water by liquid chromatography and diode array detection is reported. The method involves a modified precolumn switching methodology replacing the small precolumn with a short C18 liquid chromatography column (50mm×4.6mm,5mparticle size), thus allowing the preconcent ration of large water sample volumes whereas interferences eluting at the first of the chromatogram were discarded to waste. This approach allowed to preconcentrate 30mL river water samples, modified with 0.4% MeOH, achieving univariate method detection and determination limits ranged between 0.03 and 0.16gL−1 and between 0.2 and 0.5gL−1, respectively, with precision values lower than 9.4% for spiking levels at the quantitation limits of each analyte and lower than 4.0%, except bisoprolol (8.3%), for higherspiking levels (1.0gL−1 of all analytes). Matrix background was reduced in three way data by a baseline correction following the Eilers methodology, whereas multivariate curve resolution and alternating least squares in combination with the standard addition calibration method, applied to these data, coped with overlapping peak, systematic (additive) and proportional (matrix effect) errors. The method was successfully applied for the determination of the target pharmaceuticals in river water from three places in a river stream with acceptable recoveries and precision values, taking into account the complexity of the analytical problem. The joint statistical test for the slope and the intercept of the linear regression between the nominal concentration values versus those predicted, showed that the region computed contained the theoretically expected values (0) for the intercept and (1) for the slope (at a confidence level of 95%), which indicates the absence of both constant and proportional errors in the predicted concentrations.Fil: Martínez Galera, M.. Universidad de Almería.; EspañaFil: Gil García, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones en Humanidades y Ciencias Sociales. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación. Instituto de Investigaciones en Humanidades y Ciencias Sociales; ArgentinaFil: Culzoni, Maria Julia. Universidad Nacional del Litoral; ArgentinaFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; ArgentinaElsevier Science2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103399Martínez Galera, M.; Gil García, Magdalena; Culzoni, Maria Julia; Goicoechea, Hector Casimiro; Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated approach to green analytical methodologies; Elsevier Science; Journal of Chromatography - A; 1217; 13; 3-2010; 2042-20490021-9673CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2010.01.082info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:11Zoai:ri.conicet.gov.ar:11336/103399instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:11.406CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated approach to green analytical methodologies
title	Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated approach to green analytical methodologies
spellingShingle	Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated approach to green analytical methodologies Martínez Galera, M. MCR ALS CHROMATOGRAPHY EMERGENT CONTAMINANT
title_short	Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated approach to green analytical methodologies
title_full	Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated approach to green analytical methodologies
title_fullStr	Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated approach to green analytical methodologies
title_full_unstemmed	Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated approach to green analytical methodologies
title_sort	Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated approach to green analytical methodologies
dc.creator.none.fl_str_mv	Martínez Galera, M. Gil García, Magdalena Culzoni, Maria Julia Goicoechea, Hector Casimiro
author	Martínez Galera, M.
author_facet	Martínez Galera, M. Gil García, Magdalena Culzoni, Maria Julia Goicoechea, Hector Casimiro
author_role	author
author2	Gil García, Magdalena Culzoni, Maria Julia Goicoechea, Hector Casimiro
author2_role	author author author
dc.subject.none.fl_str_mv	MCR ALS CHROMATOGRAPHY EMERGENT CONTAMINANT
topic	MCR ALS CHROMATOGRAPHY EMERGENT CONTAMINANT
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	An analytical method for the simultaneous determination of nine -blockers (sotalol atenolol, nadolol, pindolol, metoprolol, timolol, bisoprolol, propanolol and betaxolol) and two analgesics (paracetamol and phenazone) in river water by liquid chromatography and diode array detection is reported. The method involves a modified precolumn switching methodology replacing the small precolumn with a short C18 liquid chromatography column (50mm×4.6mm,5mparticle size), thus allowing the preconcent ration of large water sample volumes whereas interferences eluting at the first of the chromatogram were discarded to waste. This approach allowed to preconcentrate 30mL river water samples, modified with 0.4% MeOH, achieving univariate method detection and determination limits ranged between 0.03 and 0.16gL−1 and between 0.2 and 0.5gL−1, respectively, with precision values lower than 9.4% for spiking levels at the quantitation limits of each analyte and lower than 4.0%, except bisoprolol (8.3%), for higherspiking levels (1.0gL−1 of all analytes). Matrix background was reduced in three way data by a baseline correction following the Eilers methodology, whereas multivariate curve resolution and alternating least squares in combination with the standard addition calibration method, applied to these data, coped with overlapping peak, systematic (additive) and proportional (matrix effect) errors. The method was successfully applied for the determination of the target pharmaceuticals in river water from three places in a river stream with acceptable recoveries and precision values, taking into account the complexity of the analytical problem. The joint statistical test for the slope and the intercept of the linear regression between the nominal concentration values versus those predicted, showed that the region computed contained the theoretically expected values (0) for the intercept and (1) for the slope (at a confidence level of 95%), which indicates the absence of both constant and proportional errors in the predicted concentrations. Fil: Martínez Galera, M.. Universidad de Almería.; España Fil: Gil García, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones en Humanidades y Ciencias Sociales. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación. Instituto de Investigaciones en Humanidades y Ciencias Sociales; Argentina Fil: Culzoni, Maria Julia. Universidad Nacional del Litoral; Argentina Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina
description	An analytical method for the simultaneous determination of nine -blockers (sotalol atenolol, nadolol, pindolol, metoprolol, timolol, bisoprolol, propanolol and betaxolol) and two analgesics (paracetamol and phenazone) in river water by liquid chromatography and diode array detection is reported. The method involves a modified precolumn switching methodology replacing the small precolumn with a short C18 liquid chromatography column (50mm×4.6mm,5mparticle size), thus allowing the preconcent ration of large water sample volumes whereas interferences eluting at the first of the chromatogram were discarded to waste. This approach allowed to preconcentrate 30mL river water samples, modified with 0.4% MeOH, achieving univariate method detection and determination limits ranged between 0.03 and 0.16gL−1 and between 0.2 and 0.5gL−1, respectively, with precision values lower than 9.4% for spiking levels at the quantitation limits of each analyte and lower than 4.0%, except bisoprolol (8.3%), for higherspiking levels (1.0gL−1 of all analytes). Matrix background was reduced in three way data by a baseline correction following the Eilers methodology, whereas multivariate curve resolution and alternating least squares in combination with the standard addition calibration method, applied to these data, coped with overlapping peak, systematic (additive) and proportional (matrix effect) errors. The method was successfully applied for the determination of the target pharmaceuticals in river water from three places in a river stream with acceptable recoveries and precision values, taking into account the complexity of the analytical problem. The joint statistical test for the slope and the intercept of the linear regression between the nominal concentration values versus those predicted, showed that the region computed contained the theoretically expected values (0) for the intercept and (1) for the slope (at a confidence level of 95%), which indicates the absence of both constant and proportional errors in the predicted concentrations.
publishDate	2010
dc.date.none.fl_str_mv	2010-03
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/103399 Martínez Galera, M.; Gil García, Magdalena; Culzoni, Maria Julia; Goicoechea, Hector Casimiro; Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated approach to green analytical methodologies; Elsevier Science; Journal of Chromatography - A; 1217; 13; 3-2010; 2042-2049 0021-9673 CONICET Digital CONICET
url	http://hdl.handle.net/11336/103399
identifier_str_mv	Martínez Galera, M.; Gil García, Magdalena; Culzoni, Maria Julia; Goicoechea, Hector Casimiro; Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated approach to green analytical methodologies; Elsevier Science; Journal of Chromatography - A; 1217; 13; 3-2010; 2042-2049 0021-9673 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2010.01.082
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Elsevier Science
publisher.none.fl_str_mv	Elsevier Science
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
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repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Determination of pharmaceuticals in river water by column switching of large sample volumes and liquid chromatography–diode array detection, assisted by chemometrics: An integrated...

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