The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis

Autores
Fernandez Bidondo, Laura; Almasia, Natalia Ines; Bazzini, Ariel Alejandro; Colombo, Roxana; Hopp, E.; Vazquez Rovere, Cecilia; Godeas, Alicia Margarita
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The biological control of fungal diseases through the use of genetically modified (GM) plants could decrease the input of chemical pesticides. To overcome possible losses in potato (Solanum tuberosum) yield because of susceptibility to soil fungal pathogens, researchers have developed potato transgenic lines expressing antifungal proteins. However, all GM crops must be monitored in their potentially detrimental effects on non-target soil microorganisms. Arbuscular mycorrhizal (AM) fungi are good candidates for this type of analysis, as good indicators of a normal rhizosphere structure and functionality. In this work, we have monitored potato lines with over-expression of genes encoding peptides with antifungal properties on their effects on the soil-borne fungal pathogen Rhizoctonia solani and AM fungi.The six GM potato lines (AG-1, AG-3, RC-1, RC-5, AGRC-8 and AGRC-12) evaluated showed higher reduction in infection indexes in comparison to untransformed plants when challenged with a highly virulent strain of R. solani. The growth of RC-1, RC-5 and AGRC-12 lines remained almost unaltered by the pathogen; which evidenced the maximum inhibition of R. solani infection. The level of root colonization by the AM fungus Rizophagus intraradices (pure in vitro isolated) did not significantly differ between transgenic and wild potato lines under in vitro and microcosm conditions. An increase in mycorrhization was evident with the addition of potato biomass residues of these GM lines in comparison to the addition of residues of the wild type potato line.In addition to the R. intraradices assays, we performed microcosm assays with soil samples from sites with at least100-year history of potato crop as inoculum source.The roots of AGRC-12 GM line showed significant higher levels of native mycorrhization and arbuscules development. In general, the potato lines apparently were less receptive to R. intraradices pure inoculum than to AM species from the natural inoculum. In this work, the selected GM potato lines did not have evident adverse effects on AM fungal colonization.
Fil: Fernandez Bidondo, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bazzini, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colombo, Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Hopp, E.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Godeas, Alicia Margarita. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
TRANSGENIC POTATO
ANTIFUNGAL GENES
RHIZOCTONIA SOLANI
ARBUSCULAR MYCORRHIZAL FUNGI
INTRARADICAL VARIATIONS
TRANSGENIC POTATO
ANTIFUNGAL GENES
RHIZOCTONIA SOLANI
ARBUSCULAR MYCORRHIZAL FUNGI
INTRARADICAL VARIATIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/118937
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/118937
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosisFernandez Bidondo, LauraAlmasia, Natalia InesBazzini, Ariel AlejandroColombo, RoxanaHopp, E.Vazquez Rovere, CeciliaGodeas, Alicia MargaritaTRANSGENIC POTATOANTIFUNGAL GENESRHIZOCTONIA SOLANIARBUSCULAR MYCORRHIZAL FUNGIINTRARADICAL VARIATIONSTRANSGENIC POTATOANTIFUNGAL GENESRHIZOCTONIA SOLANIARBUSCULAR MYCORRHIZAL FUNGIINTRARADICAL VARIATIONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The biological control of fungal diseases through the use of genetically modified (GM) plants could decrease the input of chemical pesticides. To overcome possible losses in potato (Solanum tuberosum) yield because of susceptibility to soil fungal pathogens, researchers have developed potato transgenic lines expressing antifungal proteins. However, all GM crops must be monitored in their potentially detrimental effects on non-target soil microorganisms. Arbuscular mycorrhizal (AM) fungi are good candidates for this type of analysis, as good indicators of a normal rhizosphere structure and functionality. In this work, we have monitored potato lines with over-expression of genes encoding peptides with antifungal properties on their effects on the soil-borne fungal pathogen Rhizoctonia solani and AM fungi.The six GM potato lines (AG-1, AG-3, RC-1, RC-5, AGRC-8 and AGRC-12) evaluated showed higher reduction in infection indexes in comparison to untransformed plants when challenged with a highly virulent strain of R. solani. The growth of RC-1, RC-5 and AGRC-12 lines remained almost unaltered by the pathogen; which evidenced the maximum inhibition of R. solani infection. The level of root colonization by the AM fungus Rizophagus intraradices (pure in vitro isolated) did not significantly differ between transgenic and wild potato lines under in vitro and microcosm conditions. An increase in mycorrhization was evident with the addition of potato biomass residues of these GM lines in comparison to the addition of residues of the wild type potato line.In addition to the R. intraradices assays, we performed microcosm assays with soil samples from sites with at least100-year history of potato crop as inoculum source.The roots of AGRC-12 GM line showed significant higher levels of native mycorrhization and arbuscules development. In general, the potato lines apparently were less receptive to R. intraradices pure inoculum than to AM species from the natural inoculum. In this work, the selected GM potato lines did not have evident adverse effects on AM fungal colonization.Fil: Fernandez Bidondo, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bazzini, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Colombo, Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Hopp, E.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Godeas, Alicia Margarita. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118937Fernandez Bidondo, Laura; Almasia, Natalia Ines; Bazzini, Ariel Alejandro; Colombo, Roxana; Hopp, E.; et al.; The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis; Elsevier; Crop Protection; 124; 10-2019; 1-250261-2194CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0261219419301772info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cropro.2019.05.031info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:45Zoai:ri.conicet.gov.ar:11336/118937instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:45.997CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis
title The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis
spellingShingle The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis
Fernandez Bidondo, Laura
TRANSGENIC POTATO
ANTIFUNGAL GENES
RHIZOCTONIA SOLANI
ARBUSCULAR MYCORRHIZAL FUNGI
INTRARADICAL VARIATIONS
TRANSGENIC POTATO
ANTIFUNGAL GENES
RHIZOCTONIA SOLANI
ARBUSCULAR MYCORRHIZAL FUNGI
INTRARADICAL VARIATIONS
title_short The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis
title_full The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis
title_fullStr The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis
title_full_unstemmed The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis
title_sort The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis
dc.creator.none.fl_str_mv Fernandez Bidondo, Laura
Almasia, Natalia Ines
Bazzini, Ariel Alejandro
Colombo, Roxana
Hopp, E.
Vazquez Rovere, Cecilia
Godeas, Alicia Margarita
author Fernandez Bidondo, Laura
author_facet Fernandez Bidondo, Laura
Almasia, Natalia Ines
Bazzini, Ariel Alejandro
Colombo, Roxana
Hopp, E.
Vazquez Rovere, Cecilia
Godeas, Alicia Margarita
author_role author
author2 Almasia, Natalia Ines
Bazzini, Ariel Alejandro
Colombo, Roxana
Hopp, E.
Vazquez Rovere, Cecilia
Godeas, Alicia Margarita
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv TRANSGENIC POTATO
ANTIFUNGAL GENES
RHIZOCTONIA SOLANI
ARBUSCULAR MYCORRHIZAL FUNGI
INTRARADICAL VARIATIONS
TRANSGENIC POTATO
ANTIFUNGAL GENES
RHIZOCTONIA SOLANI
ARBUSCULAR MYCORRHIZAL FUNGI
INTRARADICAL VARIATIONS
topic TRANSGENIC POTATO
ANTIFUNGAL GENES
RHIZOCTONIA SOLANI
ARBUSCULAR MYCORRHIZAL FUNGI
INTRARADICAL VARIATIONS
TRANSGENIC POTATO
ANTIFUNGAL GENES
RHIZOCTONIA SOLANI
ARBUSCULAR MYCORRHIZAL FUNGI
INTRARADICAL VARIATIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The biological control of fungal diseases through the use of genetically modified (GM) plants could decrease the input of chemical pesticides. To overcome possible losses in potato (Solanum tuberosum) yield because of susceptibility to soil fungal pathogens, researchers have developed potato transgenic lines expressing antifungal proteins. However, all GM crops must be monitored in their potentially detrimental effects on non-target soil microorganisms. Arbuscular mycorrhizal (AM) fungi are good candidates for this type of analysis, as good indicators of a normal rhizosphere structure and functionality. In this work, we have monitored potato lines with over-expression of genes encoding peptides with antifungal properties on their effects on the soil-borne fungal pathogen Rhizoctonia solani and AM fungi.The six GM potato lines (AG-1, AG-3, RC-1, RC-5, AGRC-8 and AGRC-12) evaluated showed higher reduction in infection indexes in comparison to untransformed plants when challenged with a highly virulent strain of R. solani. The growth of RC-1, RC-5 and AGRC-12 lines remained almost unaltered by the pathogen; which evidenced the maximum inhibition of R. solani infection. The level of root colonization by the AM fungus Rizophagus intraradices (pure in vitro isolated) did not significantly differ between transgenic and wild potato lines under in vitro and microcosm conditions. An increase in mycorrhization was evident with the addition of potato biomass residues of these GM lines in comparison to the addition of residues of the wild type potato line.In addition to the R. intraradices assays, we performed microcosm assays with soil samples from sites with at least100-year history of potato crop as inoculum source.The roots of AGRC-12 GM line showed significant higher levels of native mycorrhization and arbuscules development. In general, the potato lines apparently were less receptive to R. intraradices pure inoculum than to AM species from the natural inoculum. In this work, the selected GM potato lines did not have evident adverse effects on AM fungal colonization.
Fil: Fernandez Bidondo, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bazzini, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colombo, Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Hopp, E.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Godeas, Alicia Margarita. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The biological control of fungal diseases through the use of genetically modified (GM) plants could decrease the input of chemical pesticides. To overcome possible losses in potato (Solanum tuberosum) yield because of susceptibility to soil fungal pathogens, researchers have developed potato transgenic lines expressing antifungal proteins. However, all GM crops must be monitored in their potentially detrimental effects on non-target soil microorganisms. Arbuscular mycorrhizal (AM) fungi are good candidates for this type of analysis, as good indicators of a normal rhizosphere structure and functionality. In this work, we have monitored potato lines with over-expression of genes encoding peptides with antifungal properties on their effects on the soil-borne fungal pathogen Rhizoctonia solani and AM fungi.The six GM potato lines (AG-1, AG-3, RC-1, RC-5, AGRC-8 and AGRC-12) evaluated showed higher reduction in infection indexes in comparison to untransformed plants when challenged with a highly virulent strain of R. solani. The growth of RC-1, RC-5 and AGRC-12 lines remained almost unaltered by the pathogen; which evidenced the maximum inhibition of R. solani infection. The level of root colonization by the AM fungus Rizophagus intraradices (pure in vitro isolated) did not significantly differ between transgenic and wild potato lines under in vitro and microcosm conditions. An increase in mycorrhization was evident with the addition of potato biomass residues of these GM lines in comparison to the addition of residues of the wild type potato line.In addition to the R. intraradices assays, we performed microcosm assays with soil samples from sites with at least100-year history of potato crop as inoculum source.The roots of AGRC-12 GM line showed significant higher levels of native mycorrhization and arbuscules development. In general, the potato lines apparently were less receptive to R. intraradices pure inoculum than to AM species from the natural inoculum. In this work, the selected GM potato lines did not have evident adverse effects on AM fungal colonization.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/118937
Fernandez Bidondo, Laura; Almasia, Natalia Ines; Bazzini, Ariel Alejandro; Colombo, Roxana; Hopp, E.; et al.; The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis; Elsevier; Crop Protection; 124; 10-2019; 1-25
0261-2194
CONICET Digital
CONICET
url http://hdl.handle.net/11336/118937
identifier_str_mv Fernandez Bidondo, Laura; Almasia, Natalia Ines; Bazzini, Ariel Alejandro; Colombo, Roxana; Hopp, E.; et al.; The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis; Elsevier; Crop Protection; 124; 10-2019; 1-25
0261-2194
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0261219419301772
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cropro.2019.05.031
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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