Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman Study

Autores
Grochol, Jana; Dronov, Roman; Lisdat, Fred; Hildebrandt, Peter; Murgida, Daniel Horacio
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Silver electrodes were covered with mixed self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA) and 11-mercaptoundecanol (MU) and subsequently coated with alternating layers of cytochrome c (Cyt) and poly(anilinesulfonic acid) (PASA). The immobilized protein is electroactive and retains its native structure. Compared to the case of systems on gold electrodes, the stability of the assembly was found to be decreased. The redox process of Cyt is accompanied by reversible oxidation-reduction of PASA as revealed by the comparative surface-enhanced resonance Raman (SERR) analysis of assemblies including Cyt and the redox-inactive apo-cytochrome c. Timeresolved SERR experiments show a fast electron exchange between the protein and the polyelectrolyte that may play a supporting role in the electric communication of thicker multilayer assemblies employed as sensors.
Fil: Grochol, Jana. Technische Universitat Berlin; Alemania
Fil: Dronov, Roman. Max Planck Institute of Colloids and Interfaces; Alemania
Fil: Lisdat, Fred. Technical University of Applied Sciences Wildau; Alemania
Fil: Hildebrandt, Peter. Technische Universitat Berlin; Alemania
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Materia
Polyelectrolytes
Cytochrome
SAMs
SERR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103459
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/103459
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman StudyGrochol, JanaDronov, RomanLisdat, FredHildebrandt, PeterMurgida, Daniel HoracioPolyelectrolytesCytochromeSAMsSERRhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Silver electrodes were covered with mixed self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA) and 11-mercaptoundecanol (MU) and subsequently coated with alternating layers of cytochrome c (Cyt) and poly(anilinesulfonic acid) (PASA). The immobilized protein is electroactive and retains its native structure. Compared to the case of systems on gold electrodes, the stability of the assembly was found to be decreased. The redox process of Cyt is accompanied by reversible oxidation-reduction of PASA as revealed by the comparative surface-enhanced resonance Raman (SERR) analysis of assemblies including Cyt and the redox-inactive apo-cytochrome c. Timeresolved SERR experiments show a fast electron exchange between the protein and the polyelectrolyte that may play a supporting role in the electric communication of thicker multilayer assemblies employed as sensors.Fil: Grochol, Jana. Technische Universitat Berlin; AlemaniaFil: Dronov, Roman. Max Planck Institute of Colloids and Interfaces; AlemaniaFil: Lisdat, Fred. Technical University of Applied Sciences Wildau; AlemaniaFil: Hildebrandt, Peter. Technische Universitat Berlin; AlemaniaFil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaAmerican Chemical Society2007-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103459Grochol, Jana; Dronov, Roman; Lisdat, Fred; Hildebrandt, Peter; Murgida, Daniel Horacio; Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman Study; American Chemical Society; Langmuir; 23; 22; 10-2007; 11289-112940743-7463CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/la701470dinfo:eu-repo/semantics/altIdentifier/doi/10.1021/la701470dinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:11Zoai:ri.conicet.gov.ar:11336/103459instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:12.003CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman Study
title Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman Study
spellingShingle Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman Study
Grochol, Jana
Polyelectrolytes
Cytochrome
SAMs
SERR
title_short Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman Study
title_full Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman Study
title_fullStr Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman Study
title_full_unstemmed Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman Study
title_sort Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman Study
dc.creator.none.fl_str_mv Grochol, Jana
Dronov, Roman
Lisdat, Fred
Hildebrandt, Peter
Murgida, Daniel Horacio
author Grochol, Jana
author_facet Grochol, Jana
Dronov, Roman
Lisdat, Fred
Hildebrandt, Peter
Murgida, Daniel Horacio
author_role author
author2 Dronov, Roman
Lisdat, Fred
Hildebrandt, Peter
Murgida, Daniel Horacio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Polyelectrolytes
Cytochrome
SAMs
SERR
topic Polyelectrolytes
Cytochrome
SAMs
SERR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Silver electrodes were covered with mixed self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA) and 11-mercaptoundecanol (MU) and subsequently coated with alternating layers of cytochrome c (Cyt) and poly(anilinesulfonic acid) (PASA). The immobilized protein is electroactive and retains its native structure. Compared to the case of systems on gold electrodes, the stability of the assembly was found to be decreased. The redox process of Cyt is accompanied by reversible oxidation-reduction of PASA as revealed by the comparative surface-enhanced resonance Raman (SERR) analysis of assemblies including Cyt and the redox-inactive apo-cytochrome c. Timeresolved SERR experiments show a fast electron exchange between the protein and the polyelectrolyte that may play a supporting role in the electric communication of thicker multilayer assemblies employed as sensors.
Fil: Grochol, Jana. Technische Universitat Berlin; Alemania
Fil: Dronov, Roman. Max Planck Institute of Colloids and Interfaces; Alemania
Fil: Lisdat, Fred. Technical University of Applied Sciences Wildau; Alemania
Fil: Hildebrandt, Peter. Technische Universitat Berlin; Alemania
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
description Silver electrodes were covered with mixed self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA) and 11-mercaptoundecanol (MU) and subsequently coated with alternating layers of cytochrome c (Cyt) and poly(anilinesulfonic acid) (PASA). The immobilized protein is electroactive and retains its native structure. Compared to the case of systems on gold electrodes, the stability of the assembly was found to be decreased. The redox process of Cyt is accompanied by reversible oxidation-reduction of PASA as revealed by the comparative surface-enhanced resonance Raman (SERR) analysis of assemblies including Cyt and the redox-inactive apo-cytochrome c. Timeresolved SERR experiments show a fast electron exchange between the protein and the polyelectrolyte that may play a supporting role in the electric communication of thicker multilayer assemblies employed as sensors.
publishDate 2007
dc.date.none.fl_str_mv 2007-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103459
Grochol, Jana; Dronov, Roman; Lisdat, Fred; Hildebrandt, Peter; Murgida, Daniel Horacio; Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman Study; American Chemical Society; Langmuir; 23; 22; 10-2007; 11289-11294
0743-7463
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103459
identifier_str_mv Grochol, Jana; Dronov, Roman; Lisdat, Fred; Hildebrandt, Peter; Murgida, Daniel Horacio; Electron Transfer in SAM/Cytochrome/Polyelectrolyte Hybrid Systems on Electrodes:  A Time-Resolved Surface-Enhanced Resonance Raman Study; American Chemical Society; Langmuir; 23; 22; 10-2007; 11289-11294
0743-7463
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/la701470d
info:eu-repo/semantics/altIdentifier/doi/10.1021/la701470d
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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