Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics

Autores
Kranich, Anja; Ly, Hoang Khoa; Hildebrandt, Peter; Murgida, Daniel Horacio
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Heterogeneous electron transfer of proteins at biomimetic interfaces is characterized by unusual distance dependences of the electron-transfer rates, whose origin has been elusive and controversial. Using a two-color, time-resolved, surface-enhanced resonance Raman spectroelectrochemical approach, we have been able to monitor simultaneously and in real time the structure, electron-transfer kinetics, and configurational fluctuations of cytochrome c electrostatically adsorbed to electrodes coated with selfassembled monolayers. Our results show that the overall electron-transfer kinetics is determined by protein dynamics rather than by tunnelling probabilities and that the protein dynamics in turn is controlled by the interfacial electric field. Implications for interprotein electron transfer at biological membranes are discussed.
Fil: Kranich, Anja. Technische Universitat Berlin; Alemania
Fil: Ly, Hoang Khoa. Technische Universitat Berlin; Alemania
Fil: Hildebrandt, Peter. Technische Universitat Berlin; Alemania
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina
Materia
SAMs
Cytochrome
SERR
Electron transfer
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102609
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102609
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein DynamicsKranich, AnjaLy, Hoang KhoaHildebrandt, PeterMurgida, Daniel HoracioSAMsCytochromeSERRElectron transferhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Heterogeneous electron transfer of proteins at biomimetic interfaces is characterized by unusual distance dependences of the electron-transfer rates, whose origin has been elusive and controversial. Using a two-color, time-resolved, surface-enhanced resonance Raman spectroelectrochemical approach, we have been able to monitor simultaneously and in real time the structure, electron-transfer kinetics, and configurational fluctuations of cytochrome c electrostatically adsorbed to electrodes coated with selfassembled monolayers. Our results show that the overall electron-transfer kinetics is determined by protein dynamics rather than by tunnelling probabilities and that the protein dynamics in turn is controlled by the interfacial electric field. Implications for interprotein electron transfer at biological membranes are discussed.Fil: Kranich, Anja. Technische Universitat Berlin; AlemaniaFil: Ly, Hoang Khoa. Technische Universitat Berlin; AlemaniaFil: Hildebrandt, Peter. Technische Universitat Berlin; AlemaniaFil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; ArgentinaAmerican Chemical Society2008-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102609Kranich, Anja; Ly, Hoang Khoa; Hildebrandt, Peter; Murgida, Daniel Horacio; Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics; American Chemical Society; Journal of the American Chemical Society; 130; 30; 7-2008; 9844-98480002-7863CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/ja8016895info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/ja8016895info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:27Zoai:ri.conicet.gov.ar:11336/102609instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:28.277CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics
title Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics
spellingShingle Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics
Kranich, Anja
SAMs
Cytochrome
SERR
Electron transfer
title_short Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics
title_full Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics
title_fullStr Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics
title_full_unstemmed Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics
title_sort Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics
dc.creator.none.fl_str_mv Kranich, Anja
Ly, Hoang Khoa
Hildebrandt, Peter
Murgida, Daniel Horacio
author Kranich, Anja
author_facet Kranich, Anja
Ly, Hoang Khoa
Hildebrandt, Peter
Murgida, Daniel Horacio
author_role author
author2 Ly, Hoang Khoa
Hildebrandt, Peter
Murgida, Daniel Horacio
author2_role author
author
author
dc.subject.none.fl_str_mv SAMs
Cytochrome
SERR
Electron transfer
topic SAMs
Cytochrome
SERR
Electron transfer
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Heterogeneous electron transfer of proteins at biomimetic interfaces is characterized by unusual distance dependences of the electron-transfer rates, whose origin has been elusive and controversial. Using a two-color, time-resolved, surface-enhanced resonance Raman spectroelectrochemical approach, we have been able to monitor simultaneously and in real time the structure, electron-transfer kinetics, and configurational fluctuations of cytochrome c electrostatically adsorbed to electrodes coated with selfassembled monolayers. Our results show that the overall electron-transfer kinetics is determined by protein dynamics rather than by tunnelling probabilities and that the protein dynamics in turn is controlled by the interfacial electric field. Implications for interprotein electron transfer at biological membranes are discussed.
Fil: Kranich, Anja. Technische Universitat Berlin; Alemania
Fil: Ly, Hoang Khoa. Technische Universitat Berlin; Alemania
Fil: Hildebrandt, Peter. Technische Universitat Berlin; Alemania
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina
description Heterogeneous electron transfer of proteins at biomimetic interfaces is characterized by unusual distance dependences of the electron-transfer rates, whose origin has been elusive and controversial. Using a two-color, time-resolved, surface-enhanced resonance Raman spectroelectrochemical approach, we have been able to monitor simultaneously and in real time the structure, electron-transfer kinetics, and configurational fluctuations of cytochrome c electrostatically adsorbed to electrodes coated with selfassembled monolayers. Our results show that the overall electron-transfer kinetics is determined by protein dynamics rather than by tunnelling probabilities and that the protein dynamics in turn is controlled by the interfacial electric field. Implications for interprotein electron transfer at biological membranes are discussed.
publishDate 2008
dc.date.none.fl_str_mv 2008-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102609
Kranich, Anja; Ly, Hoang Khoa; Hildebrandt, Peter; Murgida, Daniel Horacio; Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics; American Chemical Society; Journal of the American Chemical Society; 130; 30; 7-2008; 9844-9848
0002-7863
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102609
identifier_str_mv Kranich, Anja; Ly, Hoang Khoa; Hildebrandt, Peter; Murgida, Daniel Horacio; Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics; American Chemical Society; Journal of the American Chemical Society; 130; 30; 7-2008; 9844-9848
0002-7863
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/ja8016895
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/ja8016895
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268668555165696
score 13.13397

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