Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiation

Autores
Gonzalez Maglio, Daniel Horacio; Cela, Eliana Maiten; Ferrari, Alejandro; Leoni, Juliana
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ultraviolet radiation (UVR) effects on skin have been extensively studied. However, mitochondrial dysfunction and superoxide (O2) production have only been studied using cell cultures, which are useful models, but do not consider the crosstalk between tissues or cellular differentiation. We aimed to evaluate the usefulness of fluorescent dyes to study skin ex vivo. Mitochondrial alterations were evaluated in epidermal cells isolated from UVR-exposed mice. Furthermore, a combination of dyes and antibodies was tested to analyse specific skin cell types. UVR caused a decrease in the percentage of total cells with polarized mitochondria, but did not change the mitochondrial O2 production. However, this production was increased significantly in DiOC6+ cells. Furthermore, it was possible to evaluate the cellular damage produced to basal keratinocytes and Langerhans cells. The results show that fluorescent dyes –alone or in combination with antibodies– are useful to analyse cellular events that take place in whole organs.
Fil: Gonzalez Maglio, Daniel Horacio. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Cela, Eliana Maiten. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Ferrari, Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Leoni, Juliana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
Ultraviolet Irradiation
Skin
Basal Keratinocyte
Langerhans Cells
Mitochondrial Polarization
Superoxide Production
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13921
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13921
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiationGonzalez Maglio, Daniel HoracioCela, Eliana MaitenFerrari, AlejandroLeoni, JulianaUltraviolet IrradiationSkinBasal KeratinocyteLangerhans CellsMitochondrial PolarizationSuperoxide Productionhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Ultraviolet radiation (UVR) effects on skin have been extensively studied. However, mitochondrial dysfunction and superoxide (O2) production have only been studied using cell cultures, which are useful models, but do not consider the crosstalk between tissues or cellular differentiation. We aimed to evaluate the usefulness of fluorescent dyes to study skin ex vivo. Mitochondrial alterations were evaluated in epidermal cells isolated from UVR-exposed mice. Furthermore, a combination of dyes and antibodies was tested to analyse specific skin cell types. UVR caused a decrease in the percentage of total cells with polarized mitochondria, but did not change the mitochondrial O2 production. However, this production was increased significantly in DiOC6+ cells. Furthermore, it was possible to evaluate the cellular damage produced to basal keratinocytes and Langerhans cells. The results show that fluorescent dyes –alone or in combination with antibodies– are useful to analyse cellular events that take place in whole organs.Fil: Gonzalez Maglio, Daniel Horacio. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Cela, Eliana Maiten. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Ferrari, Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Leoni, Juliana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaWiley2011-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13921Gonzalez Maglio, Daniel Horacio; Cela, Eliana Maiten; Ferrari, Alejandro; Leoni, Juliana; Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiation; Wiley; Experimental Dermatology; 20; 11; 6-2011; 947-9500906-6705enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0625.2011.01342.x/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1600-0625.2011.01342.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:26Zoai:ri.conicet.gov.ar:11336/13921instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:27.04CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiation
title Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiation
spellingShingle Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiation
Gonzalez Maglio, Daniel Horacio
Ultraviolet Irradiation
Skin
Basal Keratinocyte
Langerhans Cells
Mitochondrial Polarization
Superoxide Production
title_short Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiation
title_full Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiation
title_fullStr Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiation
title_full_unstemmed Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiation
title_sort Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiation
dc.creator.none.fl_str_mv Gonzalez Maglio, Daniel Horacio
Cela, Eliana Maiten
Ferrari, Alejandro
Leoni, Juliana
author Gonzalez Maglio, Daniel Horacio
author_facet Gonzalez Maglio, Daniel Horacio
Cela, Eliana Maiten
Ferrari, Alejandro
Leoni, Juliana
author_role author
author2 Cela, Eliana Maiten
Ferrari, Alejandro
Leoni, Juliana
author2_role author
author
author
dc.subject.none.fl_str_mv Ultraviolet Irradiation
Skin
Basal Keratinocyte
Langerhans Cells
Mitochondrial Polarization
Superoxide Production
topic Ultraviolet Irradiation
Skin
Basal Keratinocyte
Langerhans Cells
Mitochondrial Polarization
Superoxide Production
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Ultraviolet radiation (UVR) effects on skin have been extensively studied. However, mitochondrial dysfunction and superoxide (O2) production have only been studied using cell cultures, which are useful models, but do not consider the crosstalk between tissues or cellular differentiation. We aimed to evaluate the usefulness of fluorescent dyes to study skin ex vivo. Mitochondrial alterations were evaluated in epidermal cells isolated from UVR-exposed mice. Furthermore, a combination of dyes and antibodies was tested to analyse specific skin cell types. UVR caused a decrease in the percentage of total cells with polarized mitochondria, but did not change the mitochondrial O2 production. However, this production was increased significantly in DiOC6+ cells. Furthermore, it was possible to evaluate the cellular damage produced to basal keratinocytes and Langerhans cells. The results show that fluorescent dyes –alone or in combination with antibodies– are useful to analyse cellular events that take place in whole organs.
Fil: Gonzalez Maglio, Daniel Horacio. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Cela, Eliana Maiten. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Ferrari, Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Leoni, Juliana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Ultraviolet radiation (UVR) effects on skin have been extensively studied. However, mitochondrial dysfunction and superoxide (O2) production have only been studied using cell cultures, which are useful models, but do not consider the crosstalk between tissues or cellular differentiation. We aimed to evaluate the usefulness of fluorescent dyes to study skin ex vivo. Mitochondrial alterations were evaluated in epidermal cells isolated from UVR-exposed mice. Furthermore, a combination of dyes and antibodies was tested to analyse specific skin cell types. UVR caused a decrease in the percentage of total cells with polarized mitochondria, but did not change the mitochondrial O2 production. However, this production was increased significantly in DiOC6+ cells. Furthermore, it was possible to evaluate the cellular damage produced to basal keratinocytes and Langerhans cells. The results show that fluorescent dyes –alone or in combination with antibodies– are useful to analyse cellular events that take place in whole organs.
publishDate 2011
dc.date.none.fl_str_mv 2011-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13921
Gonzalez Maglio, Daniel Horacio; Cela, Eliana Maiten; Ferrari, Alejandro; Leoni, Juliana; Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiation; Wiley; Experimental Dermatology; 20; 11; 6-2011; 947-950
0906-6705
url http://hdl.handle.net/11336/13921
identifier_str_mv Gonzalez Maglio, Daniel Horacio; Cela, Eliana Maiten; Ferrari, Alejandro; Leoni, Juliana; Mitochondrial function evaluation in epidermal cells ex vivo after ultraviolet irradiation; Wiley; Experimental Dermatology; 20; 11; 6-2011; 947-950
0906-6705
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0625.2011.01342.x/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1600-0625.2011.01342.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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