Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes

Autores
Castrillón, Patricia O.; Cardinali, Daniel Pedro; Arce, Agustín; Cutrera, Rodolfo Angel; Esquifino, Ana I.
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To examine the regulation of interferon (IFN)-γ release by cells derived from submaxillary lymph nodes of rats subjected to an acute or chronic superior cervical ganglionectomy (SCGx). Methods: A unilateral SCGx and a contralateral sham operation were performed. Twenty hours or 7 days later cells from submaxillary lymph nodes were incubated for 24 h without any additional treatment (experiment 1), after adding lipopolysaccharide or concanavalin A (experiment 2) or after adding norepinephrine (NE, 10-8 M; experiment 3). IFN-γ concentration in the culture media was measured by ELISA. Results: Compared to controls, cells obtained from lymph nodes at a time of degeneration of sympathetic nerve terminals released more IFN-γ, whereas those derived from chronically SCGx lymph nodes released less IFN-γ. Stimulation of IFN-γ release by mitogens was detectable in the innervated or acutely denervated lymph nodes, but not in chronically denervated lymph nodes. When the effect of 10-8 M NE on IFN-γ release was tested, the neurotransmitter augmented cytokine release in cells prepared from chronically denervated lymph nodes only. Conclusion: The microenvironment provided by local sympathetic nerves is essential to enable an appropriate IFN-γ release by submaxillary lymph node cells to occur. Copyright © 2001 S. Karger AG, Basel.
Fil: Castrillón, Patricia O.. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina. Pontificia Universidad Católica Argentina ; Argentina
Fil: Arce, Agustín. Universidad Complutense de Madrid; España
Fil: Cutrera, Rodolfo Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
Materia
Interferon-Γ Release
Mitogenic Responses
Norepinephrine
Submaxillary Lymph Nodes
Superior Cervical Ganglion
Sympathetic Nerves
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39261
Acceder
id CONICETDig_66f5170a284fea466421e26d18d31842
oai_identifier_str oai:ri.conicet.gov.ar:11336/39261
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodesCastrillón, Patricia O.Cardinali, Daniel PedroArce, AgustínCutrera, Rodolfo AngelEsquifino, Ana I.Interferon-Γ ReleaseMitogenic ResponsesNorepinephrineSubmaxillary Lymph NodesSuperior Cervical GanglionSympathetic Nerveshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Objective: To examine the regulation of interferon (IFN)-γ release by cells derived from submaxillary lymph nodes of rats subjected to an acute or chronic superior cervical ganglionectomy (SCGx). Methods: A unilateral SCGx and a contralateral sham operation were performed. Twenty hours or 7 days later cells from submaxillary lymph nodes were incubated for 24 h without any additional treatment (experiment 1), after adding lipopolysaccharide or concanavalin A (experiment 2) or after adding norepinephrine (NE, 10-8 M; experiment 3). IFN-γ concentration in the culture media was measured by ELISA. Results: Compared to controls, cells obtained from lymph nodes at a time of degeneration of sympathetic nerve terminals released more IFN-γ, whereas those derived from chronically SCGx lymph nodes released less IFN-γ. Stimulation of IFN-γ release by mitogens was detectable in the innervated or acutely denervated lymph nodes, but not in chronically denervated lymph nodes. When the effect of 10-8 M NE on IFN-γ release was tested, the neurotransmitter augmented cytokine release in cells prepared from chronically denervated lymph nodes only. Conclusion: The microenvironment provided by local sympathetic nerves is essential to enable an appropriate IFN-γ release by submaxillary lymph node cells to occur. Copyright © 2001 S. Karger AG, Basel.Fil: Castrillón, Patricia O.. Universidad Complutense de Madrid; EspañaFil: Cardinali, Daniel Pedro. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina. Pontificia Universidad Católica Argentina ; ArgentinaFil: Arce, Agustín. Universidad Complutense de Madrid; EspañaFil: Cutrera, Rodolfo Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; ArgentinaFil: Esquifino, Ana I.. Universidad Complutense de Madrid; EspañaKarger2000-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39261Castrillón, Patricia O.; Cardinali, Daniel Pedro; Arce, Agustín; Cutrera, Rodolfo Angel; Esquifino, Ana I.; Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes; Karger; NeuroImmunoModulation; 8; 4; 12-2000; 197-2021021-7401CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000054280info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/54280info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:00:42Zoai:ri.conicet.gov.ar:11336/39261instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:00:42.591CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes
title Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes
spellingShingle Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes
Castrillón, Patricia O.
Interferon-Γ Release
Mitogenic Responses
Norepinephrine
Submaxillary Lymph Nodes
Superior Cervical Ganglion
Sympathetic Nerves
title_short Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes
title_full Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes
title_fullStr Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes
title_full_unstemmed Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes
title_sort Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes
dc.creator.none.fl_str_mv Castrillón, Patricia O.
Cardinali, Daniel Pedro
Arce, Agustín
Cutrera, Rodolfo Angel
Esquifino, Ana I.
author Castrillón, Patricia O.
author_facet Castrillón, Patricia O.
Cardinali, Daniel Pedro
Arce, Agustín
Cutrera, Rodolfo Angel
Esquifino, Ana I.
author_role author
author2 Cardinali, Daniel Pedro
Arce, Agustín
Cutrera, Rodolfo Angel
Esquifino, Ana I.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Interferon-Γ Release
Mitogenic Responses
Norepinephrine
Submaxillary Lymph Nodes
Superior Cervical Ganglion
Sympathetic Nerves
topic Interferon-Γ Release
Mitogenic Responses
Norepinephrine
Submaxillary Lymph Nodes
Superior Cervical Ganglion
Sympathetic Nerves
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: To examine the regulation of interferon (IFN)-γ release by cells derived from submaxillary lymph nodes of rats subjected to an acute or chronic superior cervical ganglionectomy (SCGx). Methods: A unilateral SCGx and a contralateral sham operation were performed. Twenty hours or 7 days later cells from submaxillary lymph nodes were incubated for 24 h without any additional treatment (experiment 1), after adding lipopolysaccharide or concanavalin A (experiment 2) or after adding norepinephrine (NE, 10-8 M; experiment 3). IFN-γ concentration in the culture media was measured by ELISA. Results: Compared to controls, cells obtained from lymph nodes at a time of degeneration of sympathetic nerve terminals released more IFN-γ, whereas those derived from chronically SCGx lymph nodes released less IFN-γ. Stimulation of IFN-γ release by mitogens was detectable in the innervated or acutely denervated lymph nodes, but not in chronically denervated lymph nodes. When the effect of 10-8 M NE on IFN-γ release was tested, the neurotransmitter augmented cytokine release in cells prepared from chronically denervated lymph nodes only. Conclusion: The microenvironment provided by local sympathetic nerves is essential to enable an appropriate IFN-γ release by submaxillary lymph node cells to occur. Copyright © 2001 S. Karger AG, Basel.
Fil: Castrillón, Patricia O.. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina. Pontificia Universidad Católica Argentina ; Argentina
Fil: Arce, Agustín. Universidad Complutense de Madrid; España
Fil: Cutrera, Rodolfo Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
description Objective: To examine the regulation of interferon (IFN)-γ release by cells derived from submaxillary lymph nodes of rats subjected to an acute or chronic superior cervical ganglionectomy (SCGx). Methods: A unilateral SCGx and a contralateral sham operation were performed. Twenty hours or 7 days later cells from submaxillary lymph nodes were incubated for 24 h without any additional treatment (experiment 1), after adding lipopolysaccharide or concanavalin A (experiment 2) or after adding norepinephrine (NE, 10-8 M; experiment 3). IFN-γ concentration in the culture media was measured by ELISA. Results: Compared to controls, cells obtained from lymph nodes at a time of degeneration of sympathetic nerve terminals released more IFN-γ, whereas those derived from chronically SCGx lymph nodes released less IFN-γ. Stimulation of IFN-γ release by mitogens was detectable in the innervated or acutely denervated lymph nodes, but not in chronically denervated lymph nodes. When the effect of 10-8 M NE on IFN-γ release was tested, the neurotransmitter augmented cytokine release in cells prepared from chronically denervated lymph nodes only. Conclusion: The microenvironment provided by local sympathetic nerves is essential to enable an appropriate IFN-γ release by submaxillary lymph node cells to occur. Copyright © 2001 S. Karger AG, Basel.
publishDate 2000
dc.date.none.fl_str_mv 2000-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39261
Castrillón, Patricia O.; Cardinali, Daniel Pedro; Arce, Agustín; Cutrera, Rodolfo Angel; Esquifino, Ana I.; Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes; Karger; NeuroImmunoModulation; 8; 4; 12-2000; 197-202
1021-7401
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39261
identifier_str_mv Castrillón, Patricia O.; Cardinali, Daniel Pedro; Arce, Agustín; Cutrera, Rodolfo Angel; Esquifino, Ana I.; Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes; Karger; NeuroImmunoModulation; 8; 4; 12-2000; 197-202
1021-7401
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000054280
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/54280
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083146276143104
score 13.22299

Publicaciones similares