Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina

Autores
Klisch, Manfred; Sinha, Rajeshwar P.; Helbling, Eduardo Walter; Häder, Donat P.
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Natural phytoplankton assemblages from a freshwater lake in Trelew (Province of Chubut, Patagonia, Argentina) were exposed to natural solar radiation at different depths in a water basin filled with fresh water rich in humic substances. Samples were taken at regular intervals for DNA extraction and subsequent analysis of DNA damage by determining the formation of thyminedimers using an immuno-dot-blot procedure. The use of a colorimetric detection system based on the reaction of alkaline phosphatase with BCIP (5-bromo-4-chloro-3-indolyl phosphate)/NBT (nitro blue tetrazolium) proved unsatisfactory because of uneven and strong backgroundsignals. As a consequence the antibodies were stripped and the blots were reprobed successfully, using a chemoluminescence based detection system. Natural solar radiation caused an increase from 3.2 ± 0.9 thymine dimers (T^T) per mega base pair (Mbp) to 50.9 ± 3.8 T^T Mbp–1during the light period in natural phytoplankton exposed at the surface. When the UV-B component of the solar radiation was removed by a 320 nm cut-off filter, no thymine dimers were produced. After prolonged exposure at the surface, some but not total recovery occurredovernight. There was no significant DNA damage in the samples exposed at 85 cm depth (kd = 3.62 m–1 at 305 nm). The results indicate that the impinging UV-B radiation at the study site may be deleterious to the survival of phytoplankton exposed at the water surface and thus significantly alter the nutrient cycling and biomass production.
Fil: Klisch, Manfred. Friedrich Alexander Universität; Alemania
Fil: Sinha, Rajeshwar P.. Banaras Hindu University; India. Friedrich Alexander Universität; Alemania
Fil: Helbling, Eduardo Walter. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; Argentina
Fil: Häder, Donat P.. Friedrich Alexander Universität; Alemania
Materia
DNA damage
freshwater phytoplankton
repair
solar UV radiation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105042
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, ArgentinaKlisch, ManfredSinha, Rajeshwar P.Helbling, Eduardo WalterHäder, Donat P.DNA damagefreshwater phytoplanktonrepairsolar UV radiationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Natural phytoplankton assemblages from a freshwater lake in Trelew (Province of Chubut, Patagonia, Argentina) were exposed to natural solar radiation at different depths in a water basin filled with fresh water rich in humic substances. Samples were taken at regular intervals for DNA extraction and subsequent analysis of DNA damage by determining the formation of thyminedimers using an immuno-dot-blot procedure. The use of a colorimetric detection system based on the reaction of alkaline phosphatase with BCIP (5-bromo-4-chloro-3-indolyl phosphate)/NBT (nitro blue tetrazolium) proved unsatisfactory because of uneven and strong backgroundsignals. As a consequence the antibodies were stripped and the blots were reprobed successfully, using a chemoluminescence based detection system. Natural solar radiation caused an increase from 3.2 ± 0.9 thymine dimers (T^T) per mega base pair (Mbp) to 50.9 ± 3.8 T^T Mbp–1during the light period in natural phytoplankton exposed at the surface. When the UV-B component of the solar radiation was removed by a 320 nm cut-off filter, no thymine dimers were produced. After prolonged exposure at the surface, some but not total recovery occurredovernight. There was no significant DNA damage in the samples exposed at 85 cm depth (kd = 3.62 m–1 at 305 nm). The results indicate that the impinging UV-B radiation at the study site may be deleterious to the survival of phytoplankton exposed at the water surface and thus significantly alter the nutrient cycling and biomass production.Fil: Klisch, Manfred. Friedrich Alexander Universität; AlemaniaFil: Sinha, Rajeshwar P.. Banaras Hindu University; India. Friedrich Alexander Universität; AlemaniaFil: Helbling, Eduardo Walter. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; ArgentinaFil: Häder, Donat P.. Friedrich Alexander Universität; AlemaniaBirkhauser Verlag Ag2005-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105042Klisch, Manfred; Sinha, Rajeshwar P.; Helbling, Eduardo Walter; Häder, Donat P.; Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina; Birkhauser Verlag Ag; Aquatic Sciences; 67; 1; 3-2005; 72-781015-1621CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00027-004-0770-1info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00027-004-0770-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:08:16Zoai:ri.conicet.gov.ar:11336/105042instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:08:16.454CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina
title Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina
spellingShingle Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina
Klisch, Manfred
DNA damage
freshwater phytoplankton
repair
solar UV radiation
title_short Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina
title_full Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina
title_fullStr Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina
title_full_unstemmed Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina
title_sort Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina
dc.creator.none.fl_str_mv Klisch, Manfred
Sinha, Rajeshwar P.
Helbling, Eduardo Walter
Häder, Donat P.
author Klisch, Manfred
author_facet Klisch, Manfred
Sinha, Rajeshwar P.
Helbling, Eduardo Walter
Häder, Donat P.
author_role author
author2 Sinha, Rajeshwar P.
Helbling, Eduardo Walter
Häder, Donat P.
author2_role author
author
author
dc.subject.none.fl_str_mv DNA damage
freshwater phytoplankton
repair
solar UV radiation
topic DNA damage
freshwater phytoplankton
repair
solar UV radiation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Natural phytoplankton assemblages from a freshwater lake in Trelew (Province of Chubut, Patagonia, Argentina) were exposed to natural solar radiation at different depths in a water basin filled with fresh water rich in humic substances. Samples were taken at regular intervals for DNA extraction and subsequent analysis of DNA damage by determining the formation of thyminedimers using an immuno-dot-blot procedure. The use of a colorimetric detection system based on the reaction of alkaline phosphatase with BCIP (5-bromo-4-chloro-3-indolyl phosphate)/NBT (nitro blue tetrazolium) proved unsatisfactory because of uneven and strong backgroundsignals. As a consequence the antibodies were stripped and the blots were reprobed successfully, using a chemoluminescence based detection system. Natural solar radiation caused an increase from 3.2 ± 0.9 thymine dimers (T^T) per mega base pair (Mbp) to 50.9 ± 3.8 T^T Mbp–1during the light period in natural phytoplankton exposed at the surface. When the UV-B component of the solar radiation was removed by a 320 nm cut-off filter, no thymine dimers were produced. After prolonged exposure at the surface, some but not total recovery occurredovernight. There was no significant DNA damage in the samples exposed at 85 cm depth (kd = 3.62 m–1 at 305 nm). The results indicate that the impinging UV-B radiation at the study site may be deleterious to the survival of phytoplankton exposed at the water surface and thus significantly alter the nutrient cycling and biomass production.
Fil: Klisch, Manfred. Friedrich Alexander Universität; Alemania
Fil: Sinha, Rajeshwar P.. Banaras Hindu University; India. Friedrich Alexander Universität; Alemania
Fil: Helbling, Eduardo Walter. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; Argentina
Fil: Häder, Donat P.. Friedrich Alexander Universität; Alemania
description Natural phytoplankton assemblages from a freshwater lake in Trelew (Province of Chubut, Patagonia, Argentina) were exposed to natural solar radiation at different depths in a water basin filled with fresh water rich in humic substances. Samples were taken at regular intervals for DNA extraction and subsequent analysis of DNA damage by determining the formation of thyminedimers using an immuno-dot-blot procedure. The use of a colorimetric detection system based on the reaction of alkaline phosphatase with BCIP (5-bromo-4-chloro-3-indolyl phosphate)/NBT (nitro blue tetrazolium) proved unsatisfactory because of uneven and strong backgroundsignals. As a consequence the antibodies were stripped and the blots were reprobed successfully, using a chemoluminescence based detection system. Natural solar radiation caused an increase from 3.2 ± 0.9 thymine dimers (T^T) per mega base pair (Mbp) to 50.9 ± 3.8 T^T Mbp–1during the light period in natural phytoplankton exposed at the surface. When the UV-B component of the solar radiation was removed by a 320 nm cut-off filter, no thymine dimers were produced. After prolonged exposure at the surface, some but not total recovery occurredovernight. There was no significant DNA damage in the samples exposed at 85 cm depth (kd = 3.62 m–1 at 305 nm). The results indicate that the impinging UV-B radiation at the study site may be deleterious to the survival of phytoplankton exposed at the water surface and thus significantly alter the nutrient cycling and biomass production.
publishDate 2005
dc.date.none.fl_str_mv 2005-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105042
Klisch, Manfred; Sinha, Rajeshwar P.; Helbling, Eduardo Walter; Häder, Donat P.; Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina; Birkhauser Verlag Ag; Aquatic Sciences; 67; 1; 3-2005; 72-78
1015-1621
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105042
identifier_str_mv Klisch, Manfred; Sinha, Rajeshwar P.; Helbling, Eduardo Walter; Häder, Donat P.; Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina; Birkhauser Verlag Ag; Aquatic Sciences; 67; 1; 3-2005; 72-78
1015-1621
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00027-004-0770-1
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00027-004-0770-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Birkhauser Verlag Ag
publisher.none.fl_str_mv Birkhauser Verlag Ag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781412275912704
score 12.982451

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