Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamas

Autores
Rossetto, Liliana; Gallelli, Maria Florencia; Bianchi, Carolina Paula; Franco, Gabriel; Miragaya, Marcelo
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The aim of this study was to evaluate the effect of asynthetic eCG like glycoprotein on follicular development and embryo production, as an alternative to native eCGin llamas. METHODS: Twenty non-gestating and non-lactating llamas wereexamined daily by transrectal ultrasonography (tUS) to assesovarian status (MyLab One Vet, ESAOTE) until a growingfollicle with a diameter ≥7 mm, considered ovulatory in thisspecies, was observed. At that moment, females received8 μg of a GnRH analog (IV) (buserelin acetate, Gonaxal®Biogénesis Bagó, Argentina) (Day 0). On Day 3, tUS wasperformed to confirm the absence of follicles > 5 mm andthen animals were divided in two groups: eCG-N (n= 10)received 1000 IU of native eCG (IM) (Novormon®, Syntex,Argentina), and eCG-R (n= 10) received 1000 IU of asynthetic eCG like glycoprotein (IM) (Syntex, Argentina).On Day 7, all llamas were injected with 112.5 μg ofcloprostenol (IM) (Enzaprost®, Biogénesis Bagó). On Day10, the number of ovulatory follicles were determined bytUS and then llamas were mated with a male with provenfertility. Afterwards, females were injected with 8 μg ofbuserelin acetate (IV) and 24 h later, natural mating was repeated with another male, in order to minimize the male effect. On Day 18, the number of corpus luteum (CL) that developed after mating were assessed by tUS and then embryo recovery was performed by uterine flushing. The number of follicles that developed after treatment andthe number of collected embryos between groups werecompared by Mann-Whitney test. The number of CL that developed after mating between groups were comparedby unpaired t-test. Values are expressed as mean ± SEM.RESULTS: No significant differences were observed between groupsin the number of ovulatory follicles observed on Day 10(10.5±2.9 vs. 8.4±1.3 in the eCG-N and eCG-R, respectively), nor in the number of CL that developed after mating(9.2±3 vs. 8.2±1.2 in the eCG-N and eCG-R, respectively).The number of collected embryos did not show significant differences between groups (2.6±1.1 and 3.3±1.1 in theeCG-N and eCG-R, respectively). CONCLUSIONS: The synthetic eCG like glycoprotein shows a similar effect with regards to follicular development and embryo collection than the native eCG.
Fil: Rossetto, Liliana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Gallelli, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
Fil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Franco, Gabriel. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
19th International Congress on Animal Reproduction
Bologna
Italia
Universidad de Bologna
Materia
eCG
LLAMA
EMBRYO TRANSFER
SUPERSTIMULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/225273

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oai_identifier_str oai:ri.conicet.gov.ar:11336/225273
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamasRossetto, LilianaGallelli, Maria FlorenciaBianchi, Carolina PaulaFranco, GabrielMiragaya, MarceloeCGLLAMAEMBRYO TRANSFERSUPERSTIMULATIONhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to evaluate the effect of asynthetic eCG like glycoprotein on follicular development and embryo production, as an alternative to native eCGin llamas. METHODS: Twenty non-gestating and non-lactating llamas wereexamined daily by transrectal ultrasonography (tUS) to assesovarian status (MyLab One Vet, ESAOTE) until a growingfollicle with a diameter ≥7 mm, considered ovulatory in thisspecies, was observed. At that moment, females received8 μg of a GnRH analog (IV) (buserelin acetate, Gonaxal®Biogénesis Bagó, Argentina) (Day 0). On Day 3, tUS wasperformed to confirm the absence of follicles > 5 mm andthen animals were divided in two groups: eCG-N (n= 10)received 1000 IU of native eCG (IM) (Novormon®, Syntex,Argentina), and eCG-R (n= 10) received 1000 IU of asynthetic eCG like glycoprotein (IM) (Syntex, Argentina).On Day 7, all llamas were injected with 112.5 μg ofcloprostenol (IM) (Enzaprost®, Biogénesis Bagó). On Day10, the number of ovulatory follicles were determined bytUS and then llamas were mated with a male with provenfertility. Afterwards, females were injected with 8 μg ofbuserelin acetate (IV) and 24 h later, natural mating was repeated with another male, in order to minimize the male effect. On Day 18, the number of corpus luteum (CL) that developed after mating were assessed by tUS and then embryo recovery was performed by uterine flushing. The number of follicles that developed after treatment andthe number of collected embryos between groups werecompared by Mann-Whitney test. The number of CL that developed after mating between groups were comparedby unpaired t-test. Values are expressed as mean ± SEM.RESULTS: No significant differences were observed between groupsin the number of ovulatory follicles observed on Day 10(10.5±2.9 vs. 8.4±1.3 in the eCG-N and eCG-R, respectively), nor in the number of CL that developed after mating(9.2±3 vs. 8.2±1.2 in the eCG-N and eCG-R, respectively).The number of collected embryos did not show significant differences between groups (2.6±1.1 and 3.3±1.1 in theeCG-N and eCG-R, respectively). CONCLUSIONS: The synthetic eCG like glycoprotein shows a similar effect with regards to follicular development and embryo collection than the native eCG.Fil: Rossetto, Liliana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Gallelli, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; ArgentinaFil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Franco, Gabriel. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina19th International Congress on Animal ReproductionBolognaItaliaUniversidad de BolognaUniversidad de Bologna2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/225273Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamas; 19th International Congress on Animal Reproduction; Bologna; Italia; 2022; 173-173CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iris.unito.it/retrieve/f08db1bf-68d0-4fa7-896d-80abfe6967f9/ICAR%202020%2B2%20abstract-book%20T81%20pp%20135.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:21Zoai:ri.conicet.gov.ar:11336/225273instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:21.876CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamas
title Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamas
spellingShingle Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamas
Rossetto, Liliana
eCG
LLAMA
EMBRYO TRANSFER
SUPERSTIMULATION
title_short Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamas
title_full Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamas
title_fullStr Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamas
title_full_unstemmed Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamas
title_sort Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamas
dc.creator.none.fl_str_mv Rossetto, Liliana
Gallelli, Maria Florencia
Bianchi, Carolina Paula
Franco, Gabriel
Miragaya, Marcelo
author Rossetto, Liliana
author_facet Rossetto, Liliana
Gallelli, Maria Florencia
Bianchi, Carolina Paula
Franco, Gabriel
Miragaya, Marcelo
author_role author
author2 Gallelli, Maria Florencia
Bianchi, Carolina Paula
Franco, Gabriel
Miragaya, Marcelo
author2_role author
author
author
author
dc.subject.none.fl_str_mv eCG
LLAMA
EMBRYO TRANSFER
SUPERSTIMULATION
topic eCG
LLAMA
EMBRYO TRANSFER
SUPERSTIMULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to evaluate the effect of asynthetic eCG like glycoprotein on follicular development and embryo production, as an alternative to native eCGin llamas. METHODS: Twenty non-gestating and non-lactating llamas wereexamined daily by transrectal ultrasonography (tUS) to assesovarian status (MyLab One Vet, ESAOTE) until a growingfollicle with a diameter ≥7 mm, considered ovulatory in thisspecies, was observed. At that moment, females received8 μg of a GnRH analog (IV) (buserelin acetate, Gonaxal®Biogénesis Bagó, Argentina) (Day 0). On Day 3, tUS wasperformed to confirm the absence of follicles > 5 mm andthen animals were divided in two groups: eCG-N (n= 10)received 1000 IU of native eCG (IM) (Novormon®, Syntex,Argentina), and eCG-R (n= 10) received 1000 IU of asynthetic eCG like glycoprotein (IM) (Syntex, Argentina).On Day 7, all llamas were injected with 112.5 μg ofcloprostenol (IM) (Enzaprost®, Biogénesis Bagó). On Day10, the number of ovulatory follicles were determined bytUS and then llamas were mated with a male with provenfertility. Afterwards, females were injected with 8 μg ofbuserelin acetate (IV) and 24 h later, natural mating was repeated with another male, in order to minimize the male effect. On Day 18, the number of corpus luteum (CL) that developed after mating were assessed by tUS and then embryo recovery was performed by uterine flushing. The number of follicles that developed after treatment andthe number of collected embryos between groups werecompared by Mann-Whitney test. The number of CL that developed after mating between groups were comparedby unpaired t-test. Values are expressed as mean ± SEM.RESULTS: No significant differences were observed between groupsin the number of ovulatory follicles observed on Day 10(10.5±2.9 vs. 8.4±1.3 in the eCG-N and eCG-R, respectively), nor in the number of CL that developed after mating(9.2±3 vs. 8.2±1.2 in the eCG-N and eCG-R, respectively).The number of collected embryos did not show significant differences between groups (2.6±1.1 and 3.3±1.1 in theeCG-N and eCG-R, respectively). CONCLUSIONS: The synthetic eCG like glycoprotein shows a similar effect with regards to follicular development and embryo collection than the native eCG.
Fil: Rossetto, Liliana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Gallelli, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
Fil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Franco, Gabriel. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
19th International Congress on Animal Reproduction
Bologna
Italia
Universidad de Bologna
description The aim of this study was to evaluate the effect of asynthetic eCG like glycoprotein on follicular development and embryo production, as an alternative to native eCGin llamas. METHODS: Twenty non-gestating and non-lactating llamas wereexamined daily by transrectal ultrasonography (tUS) to assesovarian status (MyLab One Vet, ESAOTE) until a growingfollicle with a diameter ≥7 mm, considered ovulatory in thisspecies, was observed. At that moment, females received8 μg of a GnRH analog (IV) (buserelin acetate, Gonaxal®Biogénesis Bagó, Argentina) (Day 0). On Day 3, tUS wasperformed to confirm the absence of follicles > 5 mm andthen animals were divided in two groups: eCG-N (n= 10)received 1000 IU of native eCG (IM) (Novormon®, Syntex,Argentina), and eCG-R (n= 10) received 1000 IU of asynthetic eCG like glycoprotein (IM) (Syntex, Argentina).On Day 7, all llamas were injected with 112.5 μg ofcloprostenol (IM) (Enzaprost®, Biogénesis Bagó). On Day10, the number of ovulatory follicles were determined bytUS and then llamas were mated with a male with provenfertility. Afterwards, females were injected with 8 μg ofbuserelin acetate (IV) and 24 h later, natural mating was repeated with another male, in order to minimize the male effect. On Day 18, the number of corpus luteum (CL) that developed after mating were assessed by tUS and then embryo recovery was performed by uterine flushing. The number of follicles that developed after treatment andthe number of collected embryos between groups werecompared by Mann-Whitney test. The number of CL that developed after mating between groups were comparedby unpaired t-test. Values are expressed as mean ± SEM.RESULTS: No significant differences were observed between groupsin the number of ovulatory follicles observed on Day 10(10.5±2.9 vs. 8.4±1.3 in the eCG-N and eCG-R, respectively), nor in the number of CL that developed after mating(9.2±3 vs. 8.2±1.2 in the eCG-N and eCG-R, respectively).The number of collected embryos did not show significant differences between groups (2.6±1.1 and 3.3±1.1 in theeCG-N and eCG-R, respectively). CONCLUSIONS: The synthetic eCG like glycoprotein shows a similar effect with regards to follicular development and embryo collection than the native eCG.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/225273
Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamas; 19th International Congress on Animal Reproduction; Bologna; Italia; 2022; 173-173
CONICET Digital
CONICET
url http://hdl.handle.net/11336/225273
identifier_str_mv Effect of 1000 IU of synthetic eCG like glycoprotein on follicular development and embryo recovery in llamas; 19th International Congress on Animal Reproduction; Bologna; Italia; 2022; 173-173
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad de Bologna
publisher.none.fl_str_mv Universidad de Bologna
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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