Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donors

Autores
Zampini, Enzo German; Veiga, Maria Fernanda; Fumuso, Fernanda Gabriela; Cabido, Luciana; Neild, Deborah Margarita; Chaves, María Graciela; Miragaya, Marcelo Horacio; Trasorras, Virginia Luz
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to evaluate the application of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time natural mating in llama embryo donors. All females (n = 8) received 8 μg IM of GnRH analog (GnRHa; buserelin) on day 0, regardless of follicular status. After eight days, another GnRHa dose was administered followed by 250 μg IM PGF2α (cloprostenol). A dose of 1000 IU IM of equine chorionic gonadotrophin (eCG) was applied on day 12 and a new dose of PGF2α was administered on day 13. All embryo donors were mated with a male of proven fertility followed by a GnRHa dose on day 18. 24 h later, mating was repeated with a different male. Transcervical uterine flushing for embryo recovery was carried out on all females on day 26. Recipient females received one dose of GnRHa (day 0) two days after the first mating of embryo donor females. A 75% (6/8) of embryo donors responded to the superstimulation treatment with a range of 2 to 5 corpus luteums (CLs) on embryo recovery day. A total of 24 CLs were registered, with a mean of 4 ± 0.9 CLs per female. Embryo recovery rate was 66.7% (16/24), with a range of 0 to 4 embryos and a mean of 2.7 ± 1.5 embryos per female. Regarding quality of the recovered embryos, 56.2% were grade I, 6.2% were grade II and 37.5% were grade V (untransferable; arrested morulae). Grade I and II embryos (n = 10) were transcervically transferred into recipient females (n = 10) six days after inducing their ovulation. At 24 days after embryo transfer (ET), a 50% pregnancy rate was registered. In conclusion, a group of llama embryo donors can be synchronized and superstimulated using a fixed-time mating protocol based on GnRHa, PGF2α, and eCG without the necessity of using ultrasonography in the field.
Fil: Zampini, Enzo German. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Veiga, Maria Fernanda. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabido, Luciana. Gobierno de la Provincia de Jujuy. Dirección Provincial de Desarrollo Ganadero; Argentina
Fil: Neild, Deborah Margarita. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Chaves, María Graciela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Miragaya, Marcelo Horacio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Trasorras, Virginia Luz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
CAMELIDS
EMBRYO TRANSFER
FOLLICULAR WAVE
OVARIAN DYNAMIC
OVARIAN SUPERSTIMULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128637
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donorsZampini, Enzo GermanVeiga, Maria FernandaFumuso, Fernanda GabrielaCabido, LucianaNeild, Deborah MargaritaChaves, María GracielaMiragaya, Marcelo HoracioTrasorras, Virginia LuzCAMELIDSEMBRYO TRANSFERFOLLICULAR WAVEOVARIAN DYNAMICOVARIAN SUPERSTIMULATIONhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of the present study was to evaluate the application of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time natural mating in llama embryo donors. All females (n = 8) received 8 μg IM of GnRH analog (GnRHa; buserelin) on day 0, regardless of follicular status. After eight days, another GnRHa dose was administered followed by 250 μg IM PGF2α (cloprostenol). A dose of 1000 IU IM of equine chorionic gonadotrophin (eCG) was applied on day 12 and a new dose of PGF2α was administered on day 13. All embryo donors were mated with a male of proven fertility followed by a GnRHa dose on day 18. 24 h later, mating was repeated with a different male. Transcervical uterine flushing for embryo recovery was carried out on all females on day 26. Recipient females received one dose of GnRHa (day 0) two days after the first mating of embryo donor females. A 75% (6/8) of embryo donors responded to the superstimulation treatment with a range of 2 to 5 corpus luteums (CLs) on embryo recovery day. A total of 24 CLs were registered, with a mean of 4 ± 0.9 CLs per female. Embryo recovery rate was 66.7% (16/24), with a range of 0 to 4 embryos and a mean of 2.7 ± 1.5 embryos per female. Regarding quality of the recovered embryos, 56.2% were grade I, 6.2% were grade II and 37.5% were grade V (untransferable; arrested morulae). Grade I and II embryos (n = 10) were transcervically transferred into recipient females (n = 10) six days after inducing their ovulation. At 24 days after embryo transfer (ET), a 50% pregnancy rate was registered. In conclusion, a group of llama embryo donors can be synchronized and superstimulated using a fixed-time mating protocol based on GnRHa, PGF2α, and eCG without the necessity of using ultrasonography in the field.Fil: Zampini, Enzo German. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Veiga, Maria Fernanda. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; ArgentinaFil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabido, Luciana. Gobierno de la Provincia de Jujuy. Dirección Provincial de Desarrollo Ganadero; ArgentinaFil: Neild, Deborah Margarita. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; ArgentinaFil: Chaves, María Graciela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; ArgentinaFil: Miragaya, Marcelo Horacio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; ArgentinaFil: Trasorras, Virginia Luz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFrontiers Media S.A.2020-11-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128637Zampini, Enzo German; Veiga, Maria Fernanda; Fumuso, Fernanda Gabriela; Cabido, Luciana; Neild, Deborah Margarita; et al.; Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donors; Frontiers Media S.A.; Frontiers in Veterinary Science; 7; 595889; 12-11-2020; 1-62297-1769CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2020.595889/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2020.595889info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:47:56Zoai:ri.conicet.gov.ar:11336/128637instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:47:57.064CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donors
title Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donors
spellingShingle Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donors
Zampini, Enzo German
CAMELIDS
EMBRYO TRANSFER
FOLLICULAR WAVE
OVARIAN DYNAMIC
OVARIAN SUPERSTIMULATION
title_short Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donors
title_full Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donors
title_fullStr Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donors
title_full_unstemmed Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donors
title_sort Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donors
dc.creator.none.fl_str_mv Zampini, Enzo German
Veiga, Maria Fernanda
Fumuso, Fernanda Gabriela
Cabido, Luciana
Neild, Deborah Margarita
Chaves, María Graciela
Miragaya, Marcelo Horacio
Trasorras, Virginia Luz
author Zampini, Enzo German
author_facet Zampini, Enzo German
Veiga, Maria Fernanda
Fumuso, Fernanda Gabriela
Cabido, Luciana
Neild, Deborah Margarita
Chaves, María Graciela
Miragaya, Marcelo Horacio
Trasorras, Virginia Luz
author_role author
author2 Veiga, Maria Fernanda
Fumuso, Fernanda Gabriela
Cabido, Luciana
Neild, Deborah Margarita
Chaves, María Graciela
Miragaya, Marcelo Horacio
Trasorras, Virginia Luz
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CAMELIDS
EMBRYO TRANSFER
FOLLICULAR WAVE
OVARIAN DYNAMIC
OVARIAN SUPERSTIMULATION
topic CAMELIDS
EMBRYO TRANSFER
FOLLICULAR WAVE
OVARIAN DYNAMIC
OVARIAN SUPERSTIMULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of the present study was to evaluate the application of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time natural mating in llama embryo donors. All females (n = 8) received 8 μg IM of GnRH analog (GnRHa; buserelin) on day 0, regardless of follicular status. After eight days, another GnRHa dose was administered followed by 250 μg IM PGF2α (cloprostenol). A dose of 1000 IU IM of equine chorionic gonadotrophin (eCG) was applied on day 12 and a new dose of PGF2α was administered on day 13. All embryo donors were mated with a male of proven fertility followed by a GnRHa dose on day 18. 24 h later, mating was repeated with a different male. Transcervical uterine flushing for embryo recovery was carried out on all females on day 26. Recipient females received one dose of GnRHa (day 0) two days after the first mating of embryo donor females. A 75% (6/8) of embryo donors responded to the superstimulation treatment with a range of 2 to 5 corpus luteums (CLs) on embryo recovery day. A total of 24 CLs were registered, with a mean of 4 ± 0.9 CLs per female. Embryo recovery rate was 66.7% (16/24), with a range of 0 to 4 embryos and a mean of 2.7 ± 1.5 embryos per female. Regarding quality of the recovered embryos, 56.2% were grade I, 6.2% were grade II and 37.5% were grade V (untransferable; arrested morulae). Grade I and II embryos (n = 10) were transcervically transferred into recipient females (n = 10) six days after inducing their ovulation. At 24 days after embryo transfer (ET), a 50% pregnancy rate was registered. In conclusion, a group of llama embryo donors can be synchronized and superstimulated using a fixed-time mating protocol based on GnRHa, PGF2α, and eCG without the necessity of using ultrasonography in the field.
Fil: Zampini, Enzo German. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Veiga, Maria Fernanda. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabido, Luciana. Gobierno de la Provincia de Jujuy. Dirección Provincial de Desarrollo Ganadero; Argentina
Fil: Neild, Deborah Margarita. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Chaves, María Graciela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Miragaya, Marcelo Horacio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Trasorras, Virginia Luz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The aim of the present study was to evaluate the application of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time natural mating in llama embryo donors. All females (n = 8) received 8 μg IM of GnRH analog (GnRHa; buserelin) on day 0, regardless of follicular status. After eight days, another GnRHa dose was administered followed by 250 μg IM PGF2α (cloprostenol). A dose of 1000 IU IM of equine chorionic gonadotrophin (eCG) was applied on day 12 and a new dose of PGF2α was administered on day 13. All embryo donors were mated with a male of proven fertility followed by a GnRHa dose on day 18. 24 h later, mating was repeated with a different male. Transcervical uterine flushing for embryo recovery was carried out on all females on day 26. Recipient females received one dose of GnRHa (day 0) two days after the first mating of embryo donor females. A 75% (6/8) of embryo donors responded to the superstimulation treatment with a range of 2 to 5 corpus luteums (CLs) on embryo recovery day. A total of 24 CLs were registered, with a mean of 4 ± 0.9 CLs per female. Embryo recovery rate was 66.7% (16/24), with a range of 0 to 4 embryos and a mean of 2.7 ± 1.5 embryos per female. Regarding quality of the recovered embryos, 56.2% were grade I, 6.2% were grade II and 37.5% were grade V (untransferable; arrested morulae). Grade I and II embryos (n = 10) were transcervically transferred into recipient females (n = 10) six days after inducing their ovulation. At 24 days after embryo transfer (ET), a 50% pregnancy rate was registered. In conclusion, a group of llama embryo donors can be synchronized and superstimulated using a fixed-time mating protocol based on GnRHa, PGF2α, and eCG without the necessity of using ultrasonography in the field.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128637
Zampini, Enzo German; Veiga, Maria Fernanda; Fumuso, Fernanda Gabriela; Cabido, Luciana; Neild, Deborah Margarita; et al.; Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donors; Frontiers Media S.A.; Frontiers in Veterinary Science; 7; 595889; 12-11-2020; 1-6
2297-1769
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128637
identifier_str_mv Zampini, Enzo German; Veiga, Maria Fernanda; Fumuso, Fernanda Gabriela; Cabido, Luciana; Neild, Deborah Margarita; et al.; Development of a GnRH-PGF2α based synchronization and superstimulation protocol for fixed-time mating in llama embryo donors; Frontiers Media S.A.; Frontiers in Veterinary Science; 7; 595889; 12-11-2020; 1-6
2297-1769
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2020.595889/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2020.595889
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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