Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulators
- Autores
- Nardi, Cristina Fernanda; Villarreal, Natalia Marina; Opazo, Maria C; Martinez, Gustavo Adolfo; Moya León, Maria A.; Civello, Pedro Marcos
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Xyloglucan endotransglycosylase/hydrolase (XTH) enzymes are involved in the remodeling of plant cell wall hemicelluloses. With the aim to study the involvement of XTHs in strawberry fruit ripening, two cDNAs (FaXTH1 and FaXTH2) coding putative XTH proteins were cloned and the expression level of both genes was analyzed on different plant tissues and during ripening of three cultivars that differ in fruit firmness: Camarosa and Selva (both firm cultivars) and Toyonoka (soft cultivar). Bioinformatics and phylogenetic analysis suggest that FaXTH1 would have xyloglucan endo-transglycosylase (XET) as exclusive activity, while FaXTH2 could be either a strict XET enzyme or have both XET/XEH (xyloglucan endohydrolase) activities. The expression profile of FaXTH1 was similar during ripening of the three cultivars analyzed; and a low transcript amount was detected in early ripening stages (LG and W) coinciding with a high diminution of fruit firmness. Nevertheless, the expression level of FaXTH1 was significantly higher during ripening in all stages in Camarosa and in LG, W and 100% R in Selva, in comparison to the softer cultivar (Toyonoka). On the other hand, not significant differences on FaXTH2 expression levels were detected during strawberry fruit ripening and between cultivars. These results suggest that FaXTH1 and FaXTH2 might play different roles on hemicellulose metabolism and fruit softening in strawberry. In addition, promoter regions of both genes were cloned and the effect of plant growth regulators on FaXTH1 and FaXTH2 gene expression was evaluated. FaXTH1 and FaXTH2 gene expression was significantly up-regulated when fruit were treated with gibberellic acid and abscisic acid, and when the endogenous source of auxins was removed. On the other hand, the expression of both genes decreased significantly in fruit treated with ethylene while the opposite situation was observed in fruit treated with 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perception
Fil: Nardi, Cristina Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Villarreal, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Opazo, Maria C. Universidad de Talca; Chile
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Fundación Ciencias Exactas; Argentina
Fil: Moya León, Maria A.. Universidad de Talca; Chile
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata. Fundación Ciencias Exactas; Argentina - Materia
-
Strawberry
Softening
Xyloglucan Endotransglycosylase/Hydrolase
Plant Growth Regulator - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/24840
Ver los metadatos del registro completo
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Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulatorsNardi, Cristina FernandaVillarreal, Natalia MarinaOpazo, Maria CMartinez, Gustavo AdolfoMoya León, Maria A.Civello, Pedro MarcosStrawberrySofteningXyloglucan Endotransglycosylase/HydrolasePlant Growth RegulatorXyloglucan endotransglycosylase/hydrolase (XTH) enzymes are involved in the remodeling of plant cell wall hemicelluloses. With the aim to study the involvement of XTHs in strawberry fruit ripening, two cDNAs (FaXTH1 and FaXTH2) coding putative XTH proteins were cloned and the expression level of both genes was analyzed on different plant tissues and during ripening of three cultivars that differ in fruit firmness: Camarosa and Selva (both firm cultivars) and Toyonoka (soft cultivar). Bioinformatics and phylogenetic analysis suggest that FaXTH1 would have xyloglucan endo-transglycosylase (XET) as exclusive activity, while FaXTH2 could be either a strict XET enzyme or have both XET/XEH (xyloglucan endohydrolase) activities. The expression profile of FaXTH1 was similar during ripening of the three cultivars analyzed; and a low transcript amount was detected in early ripening stages (LG and W) coinciding with a high diminution of fruit firmness. Nevertheless, the expression level of FaXTH1 was significantly higher during ripening in all stages in Camarosa and in LG, W and 100% R in Selva, in comparison to the softer cultivar (Toyonoka). On the other hand, not significant differences on FaXTH2 expression levels were detected during strawberry fruit ripening and between cultivars. These results suggest that FaXTH1 and FaXTH2 might play different roles on hemicellulose metabolism and fruit softening in strawberry. In addition, promoter regions of both genes were cloned and the effect of plant growth regulators on FaXTH1 and FaXTH2 gene expression was evaluated. FaXTH1 and FaXTH2 gene expression was significantly up-regulated when fruit were treated with gibberellic acid and abscisic acid, and when the endogenous source of auxins was removed. On the other hand, the expression of both genes decreased significantly in fruit treated with ethylene while the opposite situation was observed in fruit treated with 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perceptionFil: Nardi, Cristina Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Villarreal, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Opazo, Maria C. Universidad de Talca; ChileFil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Fundación Ciencias Exactas; ArgentinaFil: Moya León, Maria A.. Universidad de Talca; ChileFil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata. Fundación Ciencias Exactas; ArgentinaElsevier Science2014-01-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24840Nardi, Cristina Fernanda; Villarreal, Natalia Marina; Opazo, Maria C; Martinez, Gustavo Adolfo; Moya León, Maria A.; et al.; Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulators; Elsevier Science; Scientia Horticulturae; 165; 22-1-2014; 111-1220304-4238CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2013.10.035info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S030442381300561Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:54Zoai:ri.conicet.gov.ar:11336/24840instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:54.354CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulators |
| title |
Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulators |
| spellingShingle |
Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulators Nardi, Cristina Fernanda Strawberry Softening Xyloglucan Endotransglycosylase/Hydrolase Plant Growth Regulator |
| title_short |
Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulators |
| title_full |
Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulators |
| title_fullStr |
Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulators |
| title_full_unstemmed |
Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulators |
| title_sort |
Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulators |
| dc.creator.none.fl_str_mv |
Nardi, Cristina Fernanda Villarreal, Natalia Marina Opazo, Maria C Martinez, Gustavo Adolfo Moya León, Maria A. Civello, Pedro Marcos |
| author |
Nardi, Cristina Fernanda |
| author_facet |
Nardi, Cristina Fernanda Villarreal, Natalia Marina Opazo, Maria C Martinez, Gustavo Adolfo Moya León, Maria A. Civello, Pedro Marcos |
| author_role |
author |
| author2 |
Villarreal, Natalia Marina Opazo, Maria C Martinez, Gustavo Adolfo Moya León, Maria A. Civello, Pedro Marcos |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Strawberry Softening Xyloglucan Endotransglycosylase/Hydrolase Plant Growth Regulator |
| topic |
Strawberry Softening Xyloglucan Endotransglycosylase/Hydrolase Plant Growth Regulator |
| dc.description.none.fl_txt_mv |
Xyloglucan endotransglycosylase/hydrolase (XTH) enzymes are involved in the remodeling of plant cell wall hemicelluloses. With the aim to study the involvement of XTHs in strawberry fruit ripening, two cDNAs (FaXTH1 and FaXTH2) coding putative XTH proteins were cloned and the expression level of both genes was analyzed on different plant tissues and during ripening of three cultivars that differ in fruit firmness: Camarosa and Selva (both firm cultivars) and Toyonoka (soft cultivar). Bioinformatics and phylogenetic analysis suggest that FaXTH1 would have xyloglucan endo-transglycosylase (XET) as exclusive activity, while FaXTH2 could be either a strict XET enzyme or have both XET/XEH (xyloglucan endohydrolase) activities. The expression profile of FaXTH1 was similar during ripening of the three cultivars analyzed; and a low transcript amount was detected in early ripening stages (LG and W) coinciding with a high diminution of fruit firmness. Nevertheless, the expression level of FaXTH1 was significantly higher during ripening in all stages in Camarosa and in LG, W and 100% R in Selva, in comparison to the softer cultivar (Toyonoka). On the other hand, not significant differences on FaXTH2 expression levels were detected during strawberry fruit ripening and between cultivars. These results suggest that FaXTH1 and FaXTH2 might play different roles on hemicellulose metabolism and fruit softening in strawberry. In addition, promoter regions of both genes were cloned and the effect of plant growth regulators on FaXTH1 and FaXTH2 gene expression was evaluated. FaXTH1 and FaXTH2 gene expression was significantly up-regulated when fruit were treated with gibberellic acid and abscisic acid, and when the endogenous source of auxins was removed. On the other hand, the expression of both genes decreased significantly in fruit treated with ethylene while the opposite situation was observed in fruit treated with 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perception Fil: Nardi, Cristina Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Villarreal, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Opazo, Maria C. Universidad de Talca; Chile Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Fundación Ciencias Exactas; Argentina Fil: Moya León, Maria A.. Universidad de Talca; Chile Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata. Fundación Ciencias Exactas; Argentina |
| description |
Xyloglucan endotransglycosylase/hydrolase (XTH) enzymes are involved in the remodeling of plant cell wall hemicelluloses. With the aim to study the involvement of XTHs in strawberry fruit ripening, two cDNAs (FaXTH1 and FaXTH2) coding putative XTH proteins were cloned and the expression level of both genes was analyzed on different plant tissues and during ripening of three cultivars that differ in fruit firmness: Camarosa and Selva (both firm cultivars) and Toyonoka (soft cultivar). Bioinformatics and phylogenetic analysis suggest that FaXTH1 would have xyloglucan endo-transglycosylase (XET) as exclusive activity, while FaXTH2 could be either a strict XET enzyme or have both XET/XEH (xyloglucan endohydrolase) activities. The expression profile of FaXTH1 was similar during ripening of the three cultivars analyzed; and a low transcript amount was detected in early ripening stages (LG and W) coinciding with a high diminution of fruit firmness. Nevertheless, the expression level of FaXTH1 was significantly higher during ripening in all stages in Camarosa and in LG, W and 100% R in Selva, in comparison to the softer cultivar (Toyonoka). On the other hand, not significant differences on FaXTH2 expression levels were detected during strawberry fruit ripening and between cultivars. These results suggest that FaXTH1 and FaXTH2 might play different roles on hemicellulose metabolism and fruit softening in strawberry. In addition, promoter regions of both genes were cloned and the effect of plant growth regulators on FaXTH1 and FaXTH2 gene expression was evaluated. FaXTH1 and FaXTH2 gene expression was significantly up-regulated when fruit were treated with gibberellic acid and abscisic acid, and when the endogenous source of auxins was removed. On the other hand, the expression of both genes decreased significantly in fruit treated with ethylene while the opposite situation was observed in fruit treated with 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perception |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014-01-22 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/24840 Nardi, Cristina Fernanda; Villarreal, Natalia Marina; Opazo, Maria C; Martinez, Gustavo Adolfo; Moya León, Maria A.; et al.; Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulators; Elsevier Science; Scientia Horticulturae; 165; 22-1-2014; 111-122 0304-4238 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/24840 |
| identifier_str_mv |
Nardi, Cristina Fernanda; Villarreal, Natalia Marina; Opazo, Maria C; Martinez, Gustavo Adolfo; Moya León, Maria A.; et al.; Expression of FaXTH1 and FaXTH2 genes in strawberry fruit. Cloning of promoter regions and effect of plant growth regulators; Elsevier Science; Scientia Horticulturae; 165; 22-1-2014; 111-122 0304-4238 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2013.10.035 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S030442381300561X |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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application/pdf application/pdf application/pdf application/pdf |
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Elsevier Science |
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Elsevier Science |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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