Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B

Autores
Weber Steffens, Dorothea; Hunold, Katja; Kürschner, Johanna; Giraldez Martinez, Sonia; Elumalai, Preetham; Schmidt, Dominic; Trevani, Analía Silvina; Runza, Valeria L.; Männel, Daniela N.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ficolins activate the lectin pathway of the complement system upon binding to carbohydrate patterns on pathogens. To characterize the producer cells of ficolin-B the expression of mouse ficolin-B, the orthologue of human M-ficolin, was studied in macrophages and dendritic cells during differentiation from bone marrow cells, in primary granulocytes, and during differentiation of granulocytes derived from ER-Hoxb8 cells. Expression of ficolin-B mRNA declined in all myeloid cell types to low levels during terminal differentiation. However, in contrast to macrophages and dendritic cells, ficolin-B expression was enhanced upon activation in granulocytes. High expression of ficolin-B was observed in primary immature neutrophilic CD11b+ Ly-6Cint Ly-6Ghigh granulocytes when isolated from the bone marrow, in particular during sepsis. Ficolin-B was demonstrated in lysates of primary granulocytes, ER-Hoxb8-derived granulocytes, bone marrow-derived macrophages, and dendritic cells. Native ficolin-B from cell lysates and supernatants of granulocytes activated the lectin pathway as measured by binding to MASP-2 and inducing C4 deposition. Specific staining demonstrated intra-cellular or cell associated ficolin-B protein in activated immature granulocytes deposited in a granular fashion. This study shows that ficolin-B is stored in and set free from immature granulocytic myeloid cells indicating a role in the early infection-induced cellular response of these inflammatory cells.
Fil: Weber Steffens, Dorothea. Universitat Regensburg; Alemania
Fil: Hunold, Katja. Universitat Regensburg; Alemania
Fil: Kürschner, Johanna. Universitat Regensburg; Alemania
Fil: Giraldez Martinez, Sonia. Universitat Regensburg; Alemania
Fil: Elumalai, Preetham. Universitat Regensburg; Alemania
Fil: Schmidt, Dominic. Universitat Regensburg; Alemania
Fil: Trevani, Analía Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Runza, Valeria L.. Universitat Regensburg; Alemania
Fil: Männel, Daniela N.. Universitat Regensburg; Alemania
Materia
Complement Activation
Lectin Pathway
Inflammatory Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20860
Acceder
id CONICETDig_b570e99178532de5875627edba846626
oai_identifier_str oai:ri.conicet.gov.ar:11336/20860
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immature mouse granulocytic myeloid cells are characterized by production of ficolin-BWeber Steffens, DorotheaHunold, KatjaKürschner, JohannaGiraldez Martinez, SoniaElumalai, PreethamSchmidt, DominicTrevani, Analía SilvinaRunza, Valeria L.Männel, Daniela N.Complement ActivationLectin PathwayInflammatory Cellshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Ficolins activate the lectin pathway of the complement system upon binding to carbohydrate patterns on pathogens. To characterize the producer cells of ficolin-B the expression of mouse ficolin-B, the orthologue of human M-ficolin, was studied in macrophages and dendritic cells during differentiation from bone marrow cells, in primary granulocytes, and during differentiation of granulocytes derived from ER-Hoxb8 cells. Expression of ficolin-B mRNA declined in all myeloid cell types to low levels during terminal differentiation. However, in contrast to macrophages and dendritic cells, ficolin-B expression was enhanced upon activation in granulocytes. High expression of ficolin-B was observed in primary immature neutrophilic CD11b+ Ly-6Cint Ly-6Ghigh granulocytes when isolated from the bone marrow, in particular during sepsis. Ficolin-B was demonstrated in lysates of primary granulocytes, ER-Hoxb8-derived granulocytes, bone marrow-derived macrophages, and dendritic cells. Native ficolin-B from cell lysates and supernatants of granulocytes activated the lectin pathway as measured by binding to MASP-2 and inducing C4 deposition. Specific staining demonstrated intra-cellular or cell associated ficolin-B protein in activated immature granulocytes deposited in a granular fashion. This study shows that ficolin-B is stored in and set free from immature granulocytic myeloid cells indicating a role in the early infection-induced cellular response of these inflammatory cells.Fil: Weber Steffens, Dorothea. Universitat Regensburg; AlemaniaFil: Hunold, Katja. Universitat Regensburg; AlemaniaFil: Kürschner, Johanna. Universitat Regensburg; AlemaniaFil: Giraldez Martinez, Sonia. Universitat Regensburg; AlemaniaFil: Elumalai, Preetham. Universitat Regensburg; AlemaniaFil: Schmidt, Dominic. Universitat Regensburg; AlemaniaFil: Trevani, Analía Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Runza, Valeria L.. Universitat Regensburg; AlemaniaFil: Männel, Daniela N.. Universitat Regensburg; AlemaniaElsevier2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20860Weber Steffens, Dorothea; Hunold, Katja; Kürschner, Johanna; Giraldez Martinez, Sonia ; Elumalai, Preetham; et al.; Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B; Elsevier; Molecular Immunology; 56; 4; 12-2013; 488-4960161-5890CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0161589013004343info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molimm.2013.06.015info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:06Zoai:ri.conicet.gov.ar:11336/20860instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:06.682CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B
title Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B
spellingShingle Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B
Weber Steffens, Dorothea
Complement Activation
Lectin Pathway
Inflammatory Cells
title_short Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B
title_full Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B
title_fullStr Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B
title_full_unstemmed Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B
title_sort Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B
dc.creator.none.fl_str_mv Weber Steffens, Dorothea
Hunold, Katja
Kürschner, Johanna
Giraldez Martinez, Sonia
Elumalai, Preetham
Schmidt, Dominic
Trevani, Analía Silvina
Runza, Valeria L.
Männel, Daniela N.
author Weber Steffens, Dorothea
author_facet Weber Steffens, Dorothea
Hunold, Katja
Kürschner, Johanna
Giraldez Martinez, Sonia
Elumalai, Preetham
Schmidt, Dominic
Trevani, Analía Silvina
Runza, Valeria L.
Männel, Daniela N.
author_role author
author2 Hunold, Katja
Kürschner, Johanna
Giraldez Martinez, Sonia
Elumalai, Preetham
Schmidt, Dominic
Trevani, Analía Silvina
Runza, Valeria L.
Männel, Daniela N.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Complement Activation
Lectin Pathway
Inflammatory Cells
topic Complement Activation
Lectin Pathway
Inflammatory Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Ficolins activate the lectin pathway of the complement system upon binding to carbohydrate patterns on pathogens. To characterize the producer cells of ficolin-B the expression of mouse ficolin-B, the orthologue of human M-ficolin, was studied in macrophages and dendritic cells during differentiation from bone marrow cells, in primary granulocytes, and during differentiation of granulocytes derived from ER-Hoxb8 cells. Expression of ficolin-B mRNA declined in all myeloid cell types to low levels during terminal differentiation. However, in contrast to macrophages and dendritic cells, ficolin-B expression was enhanced upon activation in granulocytes. High expression of ficolin-B was observed in primary immature neutrophilic CD11b+ Ly-6Cint Ly-6Ghigh granulocytes when isolated from the bone marrow, in particular during sepsis. Ficolin-B was demonstrated in lysates of primary granulocytes, ER-Hoxb8-derived granulocytes, bone marrow-derived macrophages, and dendritic cells. Native ficolin-B from cell lysates and supernatants of granulocytes activated the lectin pathway as measured by binding to MASP-2 and inducing C4 deposition. Specific staining demonstrated intra-cellular or cell associated ficolin-B protein in activated immature granulocytes deposited in a granular fashion. This study shows that ficolin-B is stored in and set free from immature granulocytic myeloid cells indicating a role in the early infection-induced cellular response of these inflammatory cells.
Fil: Weber Steffens, Dorothea. Universitat Regensburg; Alemania
Fil: Hunold, Katja. Universitat Regensburg; Alemania
Fil: Kürschner, Johanna. Universitat Regensburg; Alemania
Fil: Giraldez Martinez, Sonia. Universitat Regensburg; Alemania
Fil: Elumalai, Preetham. Universitat Regensburg; Alemania
Fil: Schmidt, Dominic. Universitat Regensburg; Alemania
Fil: Trevani, Analía Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Runza, Valeria L.. Universitat Regensburg; Alemania
Fil: Männel, Daniela N.. Universitat Regensburg; Alemania
description Ficolins activate the lectin pathway of the complement system upon binding to carbohydrate patterns on pathogens. To characterize the producer cells of ficolin-B the expression of mouse ficolin-B, the orthologue of human M-ficolin, was studied in macrophages and dendritic cells during differentiation from bone marrow cells, in primary granulocytes, and during differentiation of granulocytes derived from ER-Hoxb8 cells. Expression of ficolin-B mRNA declined in all myeloid cell types to low levels during terminal differentiation. However, in contrast to macrophages and dendritic cells, ficolin-B expression was enhanced upon activation in granulocytes. High expression of ficolin-B was observed in primary immature neutrophilic CD11b+ Ly-6Cint Ly-6Ghigh granulocytes when isolated from the bone marrow, in particular during sepsis. Ficolin-B was demonstrated in lysates of primary granulocytes, ER-Hoxb8-derived granulocytes, bone marrow-derived macrophages, and dendritic cells. Native ficolin-B from cell lysates and supernatants of granulocytes activated the lectin pathway as measured by binding to MASP-2 and inducing C4 deposition. Specific staining demonstrated intra-cellular or cell associated ficolin-B protein in activated immature granulocytes deposited in a granular fashion. This study shows that ficolin-B is stored in and set free from immature granulocytic myeloid cells indicating a role in the early infection-induced cellular response of these inflammatory cells.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20860
Weber Steffens, Dorothea; Hunold, Katja; Kürschner, Johanna; Giraldez Martinez, Sonia ; Elumalai, Preetham; et al.; Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B; Elsevier; Molecular Immunology; 56; 4; 12-2013; 488-496
0161-5890
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20860
identifier_str_mv Weber Steffens, Dorothea; Hunold, Katja; Kürschner, Johanna; Giraldez Martinez, Sonia ; Elumalai, Preetham; et al.; Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B; Elsevier; Molecular Immunology; 56; 4; 12-2013; 488-496
0161-5890
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0161589013004343
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molimm.2013.06.015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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