Disposition and residues of marbofloxacin in eggs of laying hens

Autores
Errecalde, Carlos; Urzúa Pizarro, Natala Francisca; Prieto, G.; Luders, C.; Liboa, Rosendo Anibal; Gramaglia, Romina Andrea
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Marbofloxacin belongs to the group of fluoroquinolones, used exclusively in domestic animals. This study was carried out with the purpose of studying the temporal disposition of marbofloxacin in edible compartments of the egg after administration of 1, 52 mg/kg of marbofloxacin in drinking water for 11 consecutive days in hens in posture peak and average weight of 1,91±0,25 kg. Birds were housed in individual cages under controlled light conditions, ambient temperature, relative humidity, water and balanced feed ad libitum. After the administration, eggs were collected daily and immediately separated into albumen and yolk, identified per bird/day and stored at-20°C until analysis. The preparative assay consisted in extraction of the analyte using 200 mg of yolk or albumen, as appropriate, in deionized water, homogenizing solution and enrofloxacin solution as internal standard. Separation and quantification were performed by HPLC by reverse phase isocratic elution with fluorescence detector, mobile phase composed of water, acetonitrile and triethylamine adjusted to pH 3. According to the peak areas of known concentrations, the concentrations of the test samples were calculated by simple linear regression. The established marbofloxacin levels are higher and more persistent in the yolk than in albumen, reaching 8 and 9 days, respectively. The disposition characteristics observed with marbofloxacin are compatible with the physico-chemical properties of the antimicrobial with the respective compartments of the egg and the time required for its formation. Data obtained from marbofloxacin depletion in egg compartments were analyzed using the EMEA WT 1.4 software and conjecturing a rigorous MRL (0,001 µg/kg), a withdrawal period of 13 and 17 days was estimated, for albumen and yolk, respectively.
Fil: Errecalde, Carlos. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Urzúa Pizarro, Natala Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Prieto, G.. Universidad Nacional de Río Cuarto; Argentina
Fil: Luders, C.. Universidad Catolica de Temuco.; Chile
Fil: Liboa, Rosendo Anibal. Universidad Nacional de Río Cuarto; Argentina
Fil: Gramaglia, Romina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Materia
EGG
MARBOFLOXACIN
RESIDUES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/172435
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/172435
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Disposition and residues of marbofloxacin in eggs of laying hensErrecalde, CarlosUrzúa Pizarro, Natala FranciscaPrieto, G.Luders, C.Liboa, Rosendo AnibalGramaglia, Romina AndreaEGGMARBOFLOXACINRESIDUEShttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4Marbofloxacin belongs to the group of fluoroquinolones, used exclusively in domestic animals. This study was carried out with the purpose of studying the temporal disposition of marbofloxacin in edible compartments of the egg after administration of 1, 52 mg/kg of marbofloxacin in drinking water for 11 consecutive days in hens in posture peak and average weight of 1,91±0,25 kg. Birds were housed in individual cages under controlled light conditions, ambient temperature, relative humidity, water and balanced feed ad libitum. After the administration, eggs were collected daily and immediately separated into albumen and yolk, identified per bird/day and stored at-20°C until analysis. The preparative assay consisted in extraction of the analyte using 200 mg of yolk or albumen, as appropriate, in deionized water, homogenizing solution and enrofloxacin solution as internal standard. Separation and quantification were performed by HPLC by reverse phase isocratic elution with fluorescence detector, mobile phase composed of water, acetonitrile and triethylamine adjusted to pH 3. According to the peak areas of known concentrations, the concentrations of the test samples were calculated by simple linear regression. The established marbofloxacin levels are higher and more persistent in the yolk than in albumen, reaching 8 and 9 days, respectively. The disposition characteristics observed with marbofloxacin are compatible with the physico-chemical properties of the antimicrobial with the respective compartments of the egg and the time required for its formation. Data obtained from marbofloxacin depletion in egg compartments were analyzed using the EMEA WT 1.4 software and conjecturing a rigorous MRL (0,001 µg/kg), a withdrawal period of 13 and 17 days was estimated, for albumen and yolk, respectively.Fil: Errecalde, Carlos. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Urzúa Pizarro, Natala Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; ArgentinaFil: Prieto, G.. Universidad Nacional de Río Cuarto; ArgentinaFil: Luders, C.. Universidad Catolica de Temuco.; ChileFil: Liboa, Rosendo Anibal. Universidad Nacional de Río Cuarto; ArgentinaFil: Gramaglia, Romina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaMedwin Publisher2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/172435Errecalde, Carlos; Urzúa Pizarro, Natala Francisca; Prieto, G.; Luders, C.; Liboa, Rosendo Anibal; et al.; Disposition and residues of marbofloxacin in eggs of laying hens; Medwin Publisher; Advances in Pharmacology & Clinical Trials; 6; 1; 1-2021; 1-72474-9214CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medwinpublishers.com/APCT/volume.php?volumeId=369&issueId=1110info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:47Zoai:ri.conicet.gov.ar:11336/172435instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:47.828CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Disposition and residues of marbofloxacin in eggs of laying hens
title Disposition and residues of marbofloxacin in eggs of laying hens
spellingShingle Disposition and residues of marbofloxacin in eggs of laying hens
Errecalde, Carlos
EGG
MARBOFLOXACIN
RESIDUES
title_short Disposition and residues of marbofloxacin in eggs of laying hens
title_full Disposition and residues of marbofloxacin in eggs of laying hens
title_fullStr Disposition and residues of marbofloxacin in eggs of laying hens
title_full_unstemmed Disposition and residues of marbofloxacin in eggs of laying hens
title_sort Disposition and residues of marbofloxacin in eggs of laying hens
dc.creator.none.fl_str_mv Errecalde, Carlos
Urzúa Pizarro, Natala Francisca
Prieto, G.
Luders, C.
Liboa, Rosendo Anibal
Gramaglia, Romina Andrea
author Errecalde, Carlos
author_facet Errecalde, Carlos
Urzúa Pizarro, Natala Francisca
Prieto, G.
Luders, C.
Liboa, Rosendo Anibal
Gramaglia, Romina Andrea
author_role author
author2 Urzúa Pizarro, Natala Francisca
Prieto, G.
Luders, C.
Liboa, Rosendo Anibal
Gramaglia, Romina Andrea
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv EGG
MARBOFLOXACIN
RESIDUES
topic EGG
MARBOFLOXACIN
RESIDUES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Marbofloxacin belongs to the group of fluoroquinolones, used exclusively in domestic animals. This study was carried out with the purpose of studying the temporal disposition of marbofloxacin in edible compartments of the egg after administration of 1, 52 mg/kg of marbofloxacin in drinking water for 11 consecutive days in hens in posture peak and average weight of 1,91±0,25 kg. Birds were housed in individual cages under controlled light conditions, ambient temperature, relative humidity, water and balanced feed ad libitum. After the administration, eggs were collected daily and immediately separated into albumen and yolk, identified per bird/day and stored at-20°C until analysis. The preparative assay consisted in extraction of the analyte using 200 mg of yolk or albumen, as appropriate, in deionized water, homogenizing solution and enrofloxacin solution as internal standard. Separation and quantification were performed by HPLC by reverse phase isocratic elution with fluorescence detector, mobile phase composed of water, acetonitrile and triethylamine adjusted to pH 3. According to the peak areas of known concentrations, the concentrations of the test samples were calculated by simple linear regression. The established marbofloxacin levels are higher and more persistent in the yolk than in albumen, reaching 8 and 9 days, respectively. The disposition characteristics observed with marbofloxacin are compatible with the physico-chemical properties of the antimicrobial with the respective compartments of the egg and the time required for its formation. Data obtained from marbofloxacin depletion in egg compartments were analyzed using the EMEA WT 1.4 software and conjecturing a rigorous MRL (0,001 µg/kg), a withdrawal period of 13 and 17 days was estimated, for albumen and yolk, respectively.
Fil: Errecalde, Carlos. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Urzúa Pizarro, Natala Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Prieto, G.. Universidad Nacional de Río Cuarto; Argentina
Fil: Luders, C.. Universidad Catolica de Temuco.; Chile
Fil: Liboa, Rosendo Anibal. Universidad Nacional de Río Cuarto; Argentina
Fil: Gramaglia, Romina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
description Marbofloxacin belongs to the group of fluoroquinolones, used exclusively in domestic animals. This study was carried out with the purpose of studying the temporal disposition of marbofloxacin in edible compartments of the egg after administration of 1, 52 mg/kg of marbofloxacin in drinking water for 11 consecutive days in hens in posture peak and average weight of 1,91±0,25 kg. Birds were housed in individual cages under controlled light conditions, ambient temperature, relative humidity, water and balanced feed ad libitum. After the administration, eggs were collected daily and immediately separated into albumen and yolk, identified per bird/day and stored at-20°C until analysis. The preparative assay consisted in extraction of the analyte using 200 mg of yolk or albumen, as appropriate, in deionized water, homogenizing solution and enrofloxacin solution as internal standard. Separation and quantification were performed by HPLC by reverse phase isocratic elution with fluorescence detector, mobile phase composed of water, acetonitrile and triethylamine adjusted to pH 3. According to the peak areas of known concentrations, the concentrations of the test samples were calculated by simple linear regression. The established marbofloxacin levels are higher and more persistent in the yolk than in albumen, reaching 8 and 9 days, respectively. The disposition characteristics observed with marbofloxacin are compatible with the physico-chemical properties of the antimicrobial with the respective compartments of the egg and the time required for its formation. Data obtained from marbofloxacin depletion in egg compartments were analyzed using the EMEA WT 1.4 software and conjecturing a rigorous MRL (0,001 µg/kg), a withdrawal period of 13 and 17 days was estimated, for albumen and yolk, respectively.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/172435
Errecalde, Carlos; Urzúa Pizarro, Natala Francisca; Prieto, G.; Luders, C.; Liboa, Rosendo Anibal; et al.; Disposition and residues of marbofloxacin in eggs of laying hens; Medwin Publisher; Advances in Pharmacology & Clinical Trials; 6; 1; 1-2021; 1-7
2474-9214
CONICET Digital
CONICET
url http://hdl.handle.net/11336/172435
identifier_str_mv Errecalde, Carlos; Urzúa Pizarro, Natala Francisca; Prieto, G.; Luders, C.; Liboa, Rosendo Anibal; et al.; Disposition and residues of marbofloxacin in eggs of laying hens; Medwin Publisher; Advances in Pharmacology & Clinical Trials; 6; 1; 1-2021; 1-7
2474-9214
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medwinpublishers.com/APCT/volume.php?volumeId=369&issueId=1110
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Medwin Publisher
publisher.none.fl_str_mv Medwin Publisher
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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