Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues

Autores
Urzúa Pizarro, Natala Francisca; Prieto, Guillermo Fermín; Luders, Carlos Fernando; Tonini, Maria Paula; Picco, Eduardo Jesús; Errecalde, Carlos
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Marbofloxacin (MBX) is an antimicrobial for exclusive veterinary use, relatively new and of which there are few analytical methods for the extraction, identification and quantification in plasma and edible tissues (muscle, liver, kidney, lung, skin) in broiler chickens. The objective of developing a simple, sensitive and efficient extraction and detection method through highperformance liquid chromatography (HPLC) with fluorescence detection. For the preparation of the samples, known concentrations of MBX, enrofloxacin (ENX) as internal standard, methanol, water and perchloric acid, were added to plasma or tissues. Chromatographic conditions were: fluorescence detector at λex 295 and λem 490 nm, mobile phase composed of deionized water, acetonitrile and triethylamine adjusted to pH 3.0. For all matrices, the method was linear (r2 = 0.99) for MAR concentrations (0.039 to 2.5 μg/ml or gr), with an average retention time for MBX in the different matrices of 3.97 ± 0.144 minutes, and 4.99 ± 0.24 minutes for the internal standard (enrofloxacin). Detection limit in all matrices was less than 0.006 μg/ml, while recoverability percentage was 89 ± 4.65% and variation coefficients in intraday-interday assay ≤ 1.4%, determining the accuracy of the study. The analytical method applied is efficient, sensitive and reliable for future residue determinations or pharmacokinetic studies of marbofloxacin in plasma, muscle, liver, kidney, skin and lung in broiler chickens.
Marbofloxacin (MBX) ialah antimikrob yang digunakan secara eksklusif bagi veterinar, secara relatifnya adalah baru dan hanya beberapa kaedah analisis bagi pengekstrakan, pengenalpastian dan kuantifikasi di dalam plasma dan tisu yang boleh di makan (otot, hati, buah pinggang, paru-paru dan kulit) di dalam ayam daging telah di perkenal. Objektif kajian adalah pembangunan kaedah pengesanan yang mudah, sensitif dan berkesan menggunakan kromatografi cecair berprestasi tinggi (HPLC) dengan pengesan pendaflour. Bagi penyediaan sampel, kepekatan MBX, enrofloxacin (ENX) sebagai piawai dalam, metanol, air dan asid perklorik ditambah ke dalam tisu plasma. Tetapan kromatografi adalah: pengesan pendaflour pada λex 295 dan λem 490 nm, fasa bergerak terdiri daripada air ternyahion, asetonitril, dan trietilamina yang diselaraskan pada pH 3.0. Bagi semua matriks sampel, kaedah adalah bersifat linear (r2 = 0.99) bagi kepekatan MBX (0.039 hingga 2.5 μg/ml atau μg/g), dengan purata masa tahanan di dalam matriks berbeza ialah 3.97 ± 0.144 minit, dan 4.99 ± 0.24 minit bagi piawai (enrofloxacin). Had pengesan di dalam semua matriks adalah lebih rendah dari nilai 0.006 μg/ml, manakala peratus perolehan semula ialah 89 ± 4.65% dan pekali variasi bagi ujian intra-hari dan inter-hari ≤ 1.4%, menjelaskan kejituan kaedah. Kaedah analisis yang digunapakai adalah efisien, sensitif, dan dipercayai bagi tujuan penentuan sisa atau kajian farmakokinetik marbofloxacin di dalam plasma, otot, hati, buah pinggang, kulit dan paru-paru ayam daging.
Fil: Urzúa Pizarro, Natala Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Prieto, Guillermo Fermín. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Luders, Carlos Fernando. Universidad Católica de Temuco; Chile
Fil: Tonini, Maria Paula. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Picco, Eduardo Jesús. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Errecalde, Carlos. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Materia
MARBOFLOXACIN
BROILER
VALIDATION
HPLC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/176438
Acceder
id CONICETDig_489b5ffbb52ec8b14c6c8aa8da384d66
oai_identifier_str oai:ri.conicet.gov.ar:11336/176438
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissuesKaedah Validasi bagi Pengesktrakan dan Penentuan Marbofloxacin di dalam Plasma dan Tisu Ayam yang Boleh Di MakanUrzúa Pizarro, Natala FranciscaPrieto, Guillermo FermínLuders, Carlos FernandoTonini, Maria PaulaPicco, Eduardo JesúsErrecalde, CarlosMARBOFLOXACINBROILERVALIDATIONHPLChttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Marbofloxacin (MBX) is an antimicrobial for exclusive veterinary use, relatively new and of which there are few analytical methods for the extraction, identification and quantification in plasma and edible tissues (muscle, liver, kidney, lung, skin) in broiler chickens. The objective of developing a simple, sensitive and efficient extraction and detection method through highperformance liquid chromatography (HPLC) with fluorescence detection. For the preparation of the samples, known concentrations of MBX, enrofloxacin (ENX) as internal standard, methanol, water and perchloric acid, were added to plasma or tissues. Chromatographic conditions were: fluorescence detector at λex 295 and λem 490 nm, mobile phase composed of deionized water, acetonitrile and triethylamine adjusted to pH 3.0. For all matrices, the method was linear (r2 = 0.99) for MAR concentrations (0.039 to 2.5 μg/ml or gr), with an average retention time for MBX in the different matrices of 3.97 ± 0.144 minutes, and 4.99 ± 0.24 minutes for the internal standard (enrofloxacin). Detection limit in all matrices was less than 0.006 μg/ml, while recoverability percentage was 89 ± 4.65% and variation coefficients in intraday-interday assay ≤ 1.4%, determining the accuracy of the study. The analytical method applied is efficient, sensitive and reliable for future residue determinations or pharmacokinetic studies of marbofloxacin in plasma, muscle, liver, kidney, skin and lung in broiler chickens.Marbofloxacin (MBX) ialah antimikrob yang digunakan secara eksklusif bagi veterinar, secara relatifnya adalah baru dan hanya beberapa kaedah analisis bagi pengekstrakan, pengenalpastian dan kuantifikasi di dalam plasma dan tisu yang boleh di makan (otot, hati, buah pinggang, paru-paru dan kulit) di dalam ayam daging telah di perkenal. Objektif kajian adalah pembangunan kaedah pengesanan yang mudah, sensitif dan berkesan menggunakan kromatografi cecair berprestasi tinggi (HPLC) dengan pengesan pendaflour. Bagi penyediaan sampel, kepekatan MBX, enrofloxacin (ENX) sebagai piawai dalam, metanol, air dan asid perklorik ditambah ke dalam tisu plasma. Tetapan kromatografi adalah: pengesan pendaflour pada λex 295 dan λem 490 nm, fasa bergerak terdiri daripada air ternyahion, asetonitril, dan trietilamina yang diselaraskan pada pH 3.0. Bagi semua matriks sampel, kaedah adalah bersifat linear (r2 = 0.99) bagi kepekatan MBX (0.039 hingga 2.5 μg/ml atau μg/g), dengan purata masa tahanan di dalam matriks berbeza ialah 3.97 ± 0.144 minit, dan 4.99 ± 0.24 minit bagi piawai (enrofloxacin). Had pengesan di dalam semua matriks adalah lebih rendah dari nilai 0.006 μg/ml, manakala peratus perolehan semula ialah 89 ± 4.65% dan pekali variasi bagi ujian intra-hari dan inter-hari ≤ 1.4%, menjelaskan kejituan kaedah. Kaedah analisis yang digunapakai adalah efisien, sensitif, dan dipercayai bagi tujuan penentuan sisa atau kajian farmakokinetik marbofloxacin di dalam plasma, otot, hati, buah pinggang, kulit dan paru-paru ayam daging.Fil: Urzúa Pizarro, Natala Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; ArgentinaFil: Prieto, Guillermo Fermín. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; ArgentinaFil: Luders, Carlos Fernando. Universidad Católica de Temuco; ChileFil: Tonini, Maria Paula. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; ArgentinaFil: Picco, Eduardo Jesús. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Errecalde, Carlos. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; ArgentinaMalaysial Analytical Science Society2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/176438Urzúa Pizarro, Natala Francisca; Prieto, Guillermo Fermín; Luders, Carlos Fernando; Tonini, Maria Paula; Picco, Eduardo Jesús; et al.; Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues; Malaysial Analytical Science Society; Malaysian Journal of Analytical Sciences; 22; 2; 4-2018; 197-2021394-2506CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ukm.my/mjas/v22_n2/pdf/Urzua_22_2_4.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.17576/mjas-2018-2202-03info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:09:04Zoai:ri.conicet.gov.ar:11336/176438instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:09:05.239CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues
Kaedah Validasi bagi Pengesktrakan dan Penentuan Marbofloxacin di dalam Plasma dan Tisu Ayam yang Boleh Di Makan
title Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues
spellingShingle Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues
Urzúa Pizarro, Natala Francisca
MARBOFLOXACIN
BROILER
VALIDATION
HPLC
title_short Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues
title_full Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues
title_fullStr Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues
title_full_unstemmed Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues
title_sort Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues
dc.creator.none.fl_str_mv Urzúa Pizarro, Natala Francisca
Prieto, Guillermo Fermín
Luders, Carlos Fernando
Tonini, Maria Paula
Picco, Eduardo Jesús
Errecalde, Carlos
author Urzúa Pizarro, Natala Francisca
author_facet Urzúa Pizarro, Natala Francisca
Prieto, Guillermo Fermín
Luders, Carlos Fernando
Tonini, Maria Paula
Picco, Eduardo Jesús
Errecalde, Carlos
author_role author
author2 Prieto, Guillermo Fermín
Luders, Carlos Fernando
Tonini, Maria Paula
Picco, Eduardo Jesús
Errecalde, Carlos
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MARBOFLOXACIN
BROILER
VALIDATION
HPLC
topic MARBOFLOXACIN
BROILER
VALIDATION
HPLC
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Marbofloxacin (MBX) is an antimicrobial for exclusive veterinary use, relatively new and of which there are few analytical methods for the extraction, identification and quantification in plasma and edible tissues (muscle, liver, kidney, lung, skin) in broiler chickens. The objective of developing a simple, sensitive and efficient extraction and detection method through highperformance liquid chromatography (HPLC) with fluorescence detection. For the preparation of the samples, known concentrations of MBX, enrofloxacin (ENX) as internal standard, methanol, water and perchloric acid, were added to plasma or tissues. Chromatographic conditions were: fluorescence detector at λex 295 and λem 490 nm, mobile phase composed of deionized water, acetonitrile and triethylamine adjusted to pH 3.0. For all matrices, the method was linear (r2 = 0.99) for MAR concentrations (0.039 to 2.5 μg/ml or gr), with an average retention time for MBX in the different matrices of 3.97 ± 0.144 minutes, and 4.99 ± 0.24 minutes for the internal standard (enrofloxacin). Detection limit in all matrices was less than 0.006 μg/ml, while recoverability percentage was 89 ± 4.65% and variation coefficients in intraday-interday assay ≤ 1.4%, determining the accuracy of the study. The analytical method applied is efficient, sensitive and reliable for future residue determinations or pharmacokinetic studies of marbofloxacin in plasma, muscle, liver, kidney, skin and lung in broiler chickens.
Marbofloxacin (MBX) ialah antimikrob yang digunakan secara eksklusif bagi veterinar, secara relatifnya adalah baru dan hanya beberapa kaedah analisis bagi pengekstrakan, pengenalpastian dan kuantifikasi di dalam plasma dan tisu yang boleh di makan (otot, hati, buah pinggang, paru-paru dan kulit) di dalam ayam daging telah di perkenal. Objektif kajian adalah pembangunan kaedah pengesanan yang mudah, sensitif dan berkesan menggunakan kromatografi cecair berprestasi tinggi (HPLC) dengan pengesan pendaflour. Bagi penyediaan sampel, kepekatan MBX, enrofloxacin (ENX) sebagai piawai dalam, metanol, air dan asid perklorik ditambah ke dalam tisu plasma. Tetapan kromatografi adalah: pengesan pendaflour pada λex 295 dan λem 490 nm, fasa bergerak terdiri daripada air ternyahion, asetonitril, dan trietilamina yang diselaraskan pada pH 3.0. Bagi semua matriks sampel, kaedah adalah bersifat linear (r2 = 0.99) bagi kepekatan MBX (0.039 hingga 2.5 μg/ml atau μg/g), dengan purata masa tahanan di dalam matriks berbeza ialah 3.97 ± 0.144 minit, dan 4.99 ± 0.24 minit bagi piawai (enrofloxacin). Had pengesan di dalam semua matriks adalah lebih rendah dari nilai 0.006 μg/ml, manakala peratus perolehan semula ialah 89 ± 4.65% dan pekali variasi bagi ujian intra-hari dan inter-hari ≤ 1.4%, menjelaskan kejituan kaedah. Kaedah analisis yang digunapakai adalah efisien, sensitif, dan dipercayai bagi tujuan penentuan sisa atau kajian farmakokinetik marbofloxacin di dalam plasma, otot, hati, buah pinggang, kulit dan paru-paru ayam daging.
Fil: Urzúa Pizarro, Natala Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Prieto, Guillermo Fermín. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Luders, Carlos Fernando. Universidad Católica de Temuco; Chile
Fil: Tonini, Maria Paula. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Picco, Eduardo Jesús. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Errecalde, Carlos. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
description Marbofloxacin (MBX) is an antimicrobial for exclusive veterinary use, relatively new and of which there are few analytical methods for the extraction, identification and quantification in plasma and edible tissues (muscle, liver, kidney, lung, skin) in broiler chickens. The objective of developing a simple, sensitive and efficient extraction and detection method through highperformance liquid chromatography (HPLC) with fluorescence detection. For the preparation of the samples, known concentrations of MBX, enrofloxacin (ENX) as internal standard, methanol, water and perchloric acid, were added to plasma or tissues. Chromatographic conditions were: fluorescence detector at λex 295 and λem 490 nm, mobile phase composed of deionized water, acetonitrile and triethylamine adjusted to pH 3.0. For all matrices, the method was linear (r2 = 0.99) for MAR concentrations (0.039 to 2.5 μg/ml or gr), with an average retention time for MBX in the different matrices of 3.97 ± 0.144 minutes, and 4.99 ± 0.24 minutes for the internal standard (enrofloxacin). Detection limit in all matrices was less than 0.006 μg/ml, while recoverability percentage was 89 ± 4.65% and variation coefficients in intraday-interday assay ≤ 1.4%, determining the accuracy of the study. The analytical method applied is efficient, sensitive and reliable for future residue determinations or pharmacokinetic studies of marbofloxacin in plasma, muscle, liver, kidney, skin and lung in broiler chickens.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/176438
Urzúa Pizarro, Natala Francisca; Prieto, Guillermo Fermín; Luders, Carlos Fernando; Tonini, Maria Paula; Picco, Eduardo Jesús; et al.; Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues; Malaysial Analytical Science Society; Malaysian Journal of Analytical Sciences; 22; 2; 4-2018; 197-202
1394-2506
CONICET Digital
CONICET
url http://hdl.handle.net/11336/176438
identifier_str_mv Urzúa Pizarro, Natala Francisca; Prieto, Guillermo Fermín; Luders, Carlos Fernando; Tonini, Maria Paula; Picco, Eduardo Jesús; et al.; Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues; Malaysial Analytical Science Society; Malaysian Journal of Analytical Sciences; 22; 2; 4-2018; 197-202
1394-2506
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ukm.my/mjas/v22_n2/pdf/Urzua_22_2_4.pdf
info:eu-repo/semantics/altIdentifier/doi/10.17576/mjas-2018-2202-03
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Malaysial Analytical Science Society
publisher.none.fl_str_mv Malaysial Analytical Science Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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