Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions

Autores
Sutka, Moira Romina; Li, Guowei; Boudet, Julie; Boursiac, Yann; Doumas, Patrick; Maurel, Christophe
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To gain insights into the natural variation of root hydraulics and its molecular components, genotypic differences related to root water transport and plasma membrane intrinsic protein (PIP) aquaporin expression were investigated in 13 natural accessions of Arabidopsis (Arabidopsis thaliana). The hydraulic conductivity of excised root systems (Lpr) showed a 2-fold variation among accessions. The contribution of aquaporins to water uptake was characterized using as inhibitors mercury, propionic acid, and azide. The aquaporin-dependent and -independent paths of water transport made variable contributions to the total hydraulic conductivity in the different accessions. The distinct suberization patterns observed among accessions were not correlated with their root hydraulic properties. Real-time reverse transcription-polymerase chain reaction revealed, by contrast, a positive overall correlation between Lpr and certain highly expressed PIP transcripts. Root hydraulic responses to salt stress were characterized in a subset of five accessions (Bulhary-1, Catania-1, Columbia-0, Dijon-M, and Monte-Tosso-0 [Mr-0]). Lpr was down-regulated in all accessions except Mr-0. In Mr-0 and Catania-1, cortical cell hydraulic conductivity was unresponsive to salt, whereas it was down-regulated in the three other accessions. By contrast, the five accessions showed qualitatively similar aquaporin transcriptional profiles in response to salt. The overall work provides clues on how hydraulic regulation allows plant adaptation to salt stress. It also shows that a wide range of root hydraulic profiles, as previously reported in various species, can be observed in a single model species. This work paves the way for a quantitative genetics analysis of root hydraulics.
Fil: Sutka, Moira Romina. Institut de Biologie Intégrative des Plantes; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Li, Guowei. Institut de Biologie Intégrative des Plantes; Francia
Fil: Boudet, Julie. Institut de Biologie Intégrative des Plantes; Francia
Fil: Boursiac, Yann. Institut de Biologie Intégrative des Plantes; Francia
Fil: Doumas, Patrick. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Fil: Maurel, Christophe. Institut de Biologie Intégrative des Plantes; Francia
Materia
Natural Variation
Root Hydraulic Conductivity
Aquaporins
Arabidopsis Thaliana
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/68151
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/68151
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditionsSutka, Moira RominaLi, GuoweiBoudet, JulieBoursiac, YannDoumas, PatrickMaurel, ChristopheNatural VariationRoot Hydraulic ConductivityAquaporinsArabidopsis Thalianahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To gain insights into the natural variation of root hydraulics and its molecular components, genotypic differences related to root water transport and plasma membrane intrinsic protein (PIP) aquaporin expression were investigated in 13 natural accessions of Arabidopsis (Arabidopsis thaliana). The hydraulic conductivity of excised root systems (Lpr) showed a 2-fold variation among accessions. The contribution of aquaporins to water uptake was characterized using as inhibitors mercury, propionic acid, and azide. The aquaporin-dependent and -independent paths of water transport made variable contributions to the total hydraulic conductivity in the different accessions. The distinct suberization patterns observed among accessions were not correlated with their root hydraulic properties. Real-time reverse transcription-polymerase chain reaction revealed, by contrast, a positive overall correlation between Lpr and certain highly expressed PIP transcripts. Root hydraulic responses to salt stress were characterized in a subset of five accessions (Bulhary-1, Catania-1, Columbia-0, Dijon-M, and Monte-Tosso-0 [Mr-0]). Lpr was down-regulated in all accessions except Mr-0. In Mr-0 and Catania-1, cortical cell hydraulic conductivity was unresponsive to salt, whereas it was down-regulated in the three other accessions. By contrast, the five accessions showed qualitatively similar aquaporin transcriptional profiles in response to salt. The overall work provides clues on how hydraulic regulation allows plant adaptation to salt stress. It also shows that a wide range of root hydraulic profiles, as previously reported in various species, can be observed in a single model species. This work paves the way for a quantitative genetics analysis of root hydraulics.Fil: Sutka, Moira Romina. Institut de Biologie Intégrative des Plantes; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Li, Guowei. Institut de Biologie Intégrative des Plantes; FranciaFil: Boudet, Julie. Institut de Biologie Intégrative des Plantes; FranciaFil: Boursiac, Yann. Institut de Biologie Intégrative des Plantes; FranciaFil: Doumas, Patrick. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; FranciaFil: Maurel, Christophe. Institut de Biologie Intégrative des Plantes; FranciaAmerican Society of Plant Biologist2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68151Sutka, Moira Romina; Li, Guowei; Boudet, Julie; Boursiac, Yann; Doumas, Patrick; et al.; Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions; American Society of Plant Biologist; Plant Physiology; 155; 3; 3-2011; 1264-12760032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/155/3/1264info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.110.163113info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3046584/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:31:30Zoai:ri.conicet.gov.ar:11336/68151instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:31:30.915CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
title Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
spellingShingle Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
Sutka, Moira Romina
Natural Variation
Root Hydraulic Conductivity
Aquaporins
Arabidopsis Thaliana
title_short Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
title_full Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
title_fullStr Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
title_full_unstemmed Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
title_sort Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
dc.creator.none.fl_str_mv Sutka, Moira Romina
Li, Guowei
Boudet, Julie
Boursiac, Yann
Doumas, Patrick
Maurel, Christophe
author Sutka, Moira Romina
author_facet Sutka, Moira Romina
Li, Guowei
Boudet, Julie
Boursiac, Yann
Doumas, Patrick
Maurel, Christophe
author_role author
author2 Li, Guowei
Boudet, Julie
Boursiac, Yann
Doumas, Patrick
Maurel, Christophe
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Natural Variation
Root Hydraulic Conductivity
Aquaporins
Arabidopsis Thaliana
topic Natural Variation
Root Hydraulic Conductivity
Aquaporins
Arabidopsis Thaliana
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To gain insights into the natural variation of root hydraulics and its molecular components, genotypic differences related to root water transport and plasma membrane intrinsic protein (PIP) aquaporin expression were investigated in 13 natural accessions of Arabidopsis (Arabidopsis thaliana). The hydraulic conductivity of excised root systems (Lpr) showed a 2-fold variation among accessions. The contribution of aquaporins to water uptake was characterized using as inhibitors mercury, propionic acid, and azide. The aquaporin-dependent and -independent paths of water transport made variable contributions to the total hydraulic conductivity in the different accessions. The distinct suberization patterns observed among accessions were not correlated with their root hydraulic properties. Real-time reverse transcription-polymerase chain reaction revealed, by contrast, a positive overall correlation between Lpr and certain highly expressed PIP transcripts. Root hydraulic responses to salt stress were characterized in a subset of five accessions (Bulhary-1, Catania-1, Columbia-0, Dijon-M, and Monte-Tosso-0 [Mr-0]). Lpr was down-regulated in all accessions except Mr-0. In Mr-0 and Catania-1, cortical cell hydraulic conductivity was unresponsive to salt, whereas it was down-regulated in the three other accessions. By contrast, the five accessions showed qualitatively similar aquaporin transcriptional profiles in response to salt. The overall work provides clues on how hydraulic regulation allows plant adaptation to salt stress. It also shows that a wide range of root hydraulic profiles, as previously reported in various species, can be observed in a single model species. This work paves the way for a quantitative genetics analysis of root hydraulics.
Fil: Sutka, Moira Romina. Institut de Biologie Intégrative des Plantes; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Li, Guowei. Institut de Biologie Intégrative des Plantes; Francia
Fil: Boudet, Julie. Institut de Biologie Intégrative des Plantes; Francia
Fil: Boursiac, Yann. Institut de Biologie Intégrative des Plantes; Francia
Fil: Doumas, Patrick. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Fil: Maurel, Christophe. Institut de Biologie Intégrative des Plantes; Francia
description To gain insights into the natural variation of root hydraulics and its molecular components, genotypic differences related to root water transport and plasma membrane intrinsic protein (PIP) aquaporin expression were investigated in 13 natural accessions of Arabidopsis (Arabidopsis thaliana). The hydraulic conductivity of excised root systems (Lpr) showed a 2-fold variation among accessions. The contribution of aquaporins to water uptake was characterized using as inhibitors mercury, propionic acid, and azide. The aquaporin-dependent and -independent paths of water transport made variable contributions to the total hydraulic conductivity in the different accessions. The distinct suberization patterns observed among accessions were not correlated with their root hydraulic properties. Real-time reverse transcription-polymerase chain reaction revealed, by contrast, a positive overall correlation between Lpr and certain highly expressed PIP transcripts. Root hydraulic responses to salt stress were characterized in a subset of five accessions (Bulhary-1, Catania-1, Columbia-0, Dijon-M, and Monte-Tosso-0 [Mr-0]). Lpr was down-regulated in all accessions except Mr-0. In Mr-0 and Catania-1, cortical cell hydraulic conductivity was unresponsive to salt, whereas it was down-regulated in the three other accessions. By contrast, the five accessions showed qualitatively similar aquaporin transcriptional profiles in response to salt. The overall work provides clues on how hydraulic regulation allows plant adaptation to salt stress. It also shows that a wide range of root hydraulic profiles, as previously reported in various species, can be observed in a single model species. This work paves the way for a quantitative genetics analysis of root hydraulics.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68151
Sutka, Moira Romina; Li, Guowei; Boudet, Julie; Boursiac, Yann; Doumas, Patrick; et al.; Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions; American Society of Plant Biologist; Plant Physiology; 155; 3; 3-2011; 1264-1276
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68151
identifier_str_mv Sutka, Moira Romina; Li, Guowei; Boudet, Julie; Boursiac, Yann; Doumas, Patrick; et al.; Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions; American Society of Plant Biologist; Plant Physiology; 155; 3; 3-2011; 1264-1276
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/155/3/1264
info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.110.163113
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3046584/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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