Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings

Autores
Xu, Hao; Kemppainen, Minna Johanna; El Kayal, Walid; Lee, Seong Hee; Pardo, Alejandro Guillermo; Cooke, Janice E.K.; Zwiazek, Janusz J.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Summary: The contribution of hyphae to water transport in ectomycorrhizal (ECM) white spruce (Picea glauca) seedlings was examined by altering expression of a major water-transporting aquaporin in Laccaria bicolor. Picea glauca was inoculated with wild-type (WT), mock transgenic or L.bicolor aquaporin JQ585595-overexpressing (OE) strains and exposed to root temperatures ranging from 5 to 20°C to examine the root water transport properties, physiological responses and plasma membrane intrinsic protein (PIP) expression in colonized plants. Mycorrhization increased shoot water potential, transpiration, net photosynthetic rates, root hydraulic conductivity and root cortical cell hydraulic conductivity in seedlings. At 20°C, OE plants had higher root hydraulic conductivity compared with WT plants and the increases were accompanied by higher expression of P.glauca PIP GQ03401_M18.1 in roots. In contrast to WT L.bicolor, the effects of OE fungi on root and root cortical cell hydraulic conductivities were abolished at 10 and 5°C in the absence of major changes in the examined transcript levels of P.glauca root PIPs. The results provide evidence for the importance of fungal aquaporins in root water transport of mycorrhizal plants. They also demonstrate links between hyphal water transport, root aquaporin expression and root water transport in ECM plants.
Fil: Xu, Hao. University of Alberta; Canadá
Fil: Kemppainen, Minna Johanna. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
Fil: El Kayal, Walid. University of Alberta; Canadá
Fil: Lee, Seong Hee. University of Alberta; Canadá
Fil: Pardo, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
Fil: Cooke, Janice E.K.. University of Alberta; Canadá
Fil: Zwiazek, Janusz J.. University of Alberta; Canadá
Materia
Cell Hydraulic Conductivity
Laccaria Bicolor
Mycorrhizal Fungal Aquaporins
Picea Glauca
Plasma Membrane Intrinsic Protein
Root Hydraulic Conductivity
Water Transport
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36516
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/36516
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlingsXu, HaoKemppainen, Minna JohannaEl Kayal, WalidLee, Seong HeePardo, Alejandro GuillermoCooke, Janice E.K.Zwiazek, Janusz J.Cell Hydraulic ConductivityLaccaria BicolorMycorrhizal Fungal AquaporinsPicea GlaucaPlasma Membrane Intrinsic ProteinRoot Hydraulic ConductivityWater Transporthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Summary: The contribution of hyphae to water transport in ectomycorrhizal (ECM) white spruce (Picea glauca) seedlings was examined by altering expression of a major water-transporting aquaporin in Laccaria bicolor. Picea glauca was inoculated with wild-type (WT), mock transgenic or L.bicolor aquaporin JQ585595-overexpressing (OE) strains and exposed to root temperatures ranging from 5 to 20°C to examine the root water transport properties, physiological responses and plasma membrane intrinsic protein (PIP) expression in colonized plants. Mycorrhization increased shoot water potential, transpiration, net photosynthetic rates, root hydraulic conductivity and root cortical cell hydraulic conductivity in seedlings. At 20°C, OE plants had higher root hydraulic conductivity compared with WT plants and the increases were accompanied by higher expression of P.glauca PIP GQ03401_M18.1 in roots. In contrast to WT L.bicolor, the effects of OE fungi on root and root cortical cell hydraulic conductivities were abolished at 10 and 5°C in the absence of major changes in the examined transcript levels of P.glauca root PIPs. The results provide evidence for the importance of fungal aquaporins in root water transport of mycorrhizal plants. They also demonstrate links between hyphal water transport, root aquaporin expression and root water transport in ECM plants.Fil: Xu, Hao. University of Alberta; CanadáFil: Kemppainen, Minna Johanna. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; ArgentinaFil: El Kayal, Walid. University of Alberta; CanadáFil: Lee, Seong Hee. University of Alberta; CanadáFil: Pardo, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; ArgentinaFil: Cooke, Janice E.K.. University of Alberta; CanadáFil: Zwiazek, Janusz J.. University of Alberta; CanadáWiley Blackwell Publishing, Inc2014-10-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36516Xu, Hao; Kemppainen, Minna Johanna; El Kayal, Walid; Lee, Seong Hee; Pardo, Alejandro Guillermo; et al.; Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings; Wiley Blackwell Publishing, Inc; New Phytologist; 205; 2; 17-10-2014; 757-7700028-646XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/nph.13098info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/nph.13098/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:52:25Zoai:ri.conicet.gov.ar:11336/36516instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:52:25.384CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings
title Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings
spellingShingle Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings
Xu, Hao
Cell Hydraulic Conductivity
Laccaria Bicolor
Mycorrhizal Fungal Aquaporins
Picea Glauca
Plasma Membrane Intrinsic Protein
Root Hydraulic Conductivity
Water Transport
title_short Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings
title_full Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings
title_fullStr Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings
title_full_unstemmed Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings
title_sort Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings
dc.creator.none.fl_str_mv Xu, Hao
Kemppainen, Minna Johanna
El Kayal, Walid
Lee, Seong Hee
Pardo, Alejandro Guillermo
Cooke, Janice E.K.
Zwiazek, Janusz J.
author Xu, Hao
author_facet Xu, Hao
Kemppainen, Minna Johanna
El Kayal, Walid
Lee, Seong Hee
Pardo, Alejandro Guillermo
Cooke, Janice E.K.
Zwiazek, Janusz J.
author_role author
author2 Kemppainen, Minna Johanna
El Kayal, Walid
Lee, Seong Hee
Pardo, Alejandro Guillermo
Cooke, Janice E.K.
Zwiazek, Janusz J.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cell Hydraulic Conductivity
Laccaria Bicolor
Mycorrhizal Fungal Aquaporins
Picea Glauca
Plasma Membrane Intrinsic Protein
Root Hydraulic Conductivity
Water Transport
topic Cell Hydraulic Conductivity
Laccaria Bicolor
Mycorrhizal Fungal Aquaporins
Picea Glauca
Plasma Membrane Intrinsic Protein
Root Hydraulic Conductivity
Water Transport
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Summary: The contribution of hyphae to water transport in ectomycorrhizal (ECM) white spruce (Picea glauca) seedlings was examined by altering expression of a major water-transporting aquaporin in Laccaria bicolor. Picea glauca was inoculated with wild-type (WT), mock transgenic or L.bicolor aquaporin JQ585595-overexpressing (OE) strains and exposed to root temperatures ranging from 5 to 20°C to examine the root water transport properties, physiological responses and plasma membrane intrinsic protein (PIP) expression in colonized plants. Mycorrhization increased shoot water potential, transpiration, net photosynthetic rates, root hydraulic conductivity and root cortical cell hydraulic conductivity in seedlings. At 20°C, OE plants had higher root hydraulic conductivity compared with WT plants and the increases were accompanied by higher expression of P.glauca PIP GQ03401_M18.1 in roots. In contrast to WT L.bicolor, the effects of OE fungi on root and root cortical cell hydraulic conductivities were abolished at 10 and 5°C in the absence of major changes in the examined transcript levels of P.glauca root PIPs. The results provide evidence for the importance of fungal aquaporins in root water transport of mycorrhizal plants. They also demonstrate links between hyphal water transport, root aquaporin expression and root water transport in ECM plants.
Fil: Xu, Hao. University of Alberta; Canadá
Fil: Kemppainen, Minna Johanna. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
Fil: El Kayal, Walid. University of Alberta; Canadá
Fil: Lee, Seong Hee. University of Alberta; Canadá
Fil: Pardo, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
Fil: Cooke, Janice E.K.. University of Alberta; Canadá
Fil: Zwiazek, Janusz J.. University of Alberta; Canadá
description Summary: The contribution of hyphae to water transport in ectomycorrhizal (ECM) white spruce (Picea glauca) seedlings was examined by altering expression of a major water-transporting aquaporin in Laccaria bicolor. Picea glauca was inoculated with wild-type (WT), mock transgenic or L.bicolor aquaporin JQ585595-overexpressing (OE) strains and exposed to root temperatures ranging from 5 to 20°C to examine the root water transport properties, physiological responses and plasma membrane intrinsic protein (PIP) expression in colonized plants. Mycorrhization increased shoot water potential, transpiration, net photosynthetic rates, root hydraulic conductivity and root cortical cell hydraulic conductivity in seedlings. At 20°C, OE plants had higher root hydraulic conductivity compared with WT plants and the increases were accompanied by higher expression of P.glauca PIP GQ03401_M18.1 in roots. In contrast to WT L.bicolor, the effects of OE fungi on root and root cortical cell hydraulic conductivities were abolished at 10 and 5°C in the absence of major changes in the examined transcript levels of P.glauca root PIPs. The results provide evidence for the importance of fungal aquaporins in root water transport of mycorrhizal plants. They also demonstrate links between hyphal water transport, root aquaporin expression and root water transport in ECM plants.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-17
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36516
Xu, Hao; Kemppainen, Minna Johanna; El Kayal, Walid; Lee, Seong Hee; Pardo, Alejandro Guillermo; et al.; Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings; Wiley Blackwell Publishing, Inc; New Phytologist; 205; 2; 17-10-2014; 757-770
0028-646X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36516
identifier_str_mv Xu, Hao; Kemppainen, Minna Johanna; El Kayal, Walid; Lee, Seong Hee; Pardo, Alejandro Guillermo; et al.; Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings; Wiley Blackwell Publishing, Inc; New Phytologist; 205; 2; 17-10-2014; 757-770
0028-646X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.13098
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/nph.13098/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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