Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine For...
- Autores
- Vignaduzzo, Silvana Edit; Kaufman, Teodoro Saul
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A simple and efficient liquid chromatographic method has been developed and validated for the simultaneous determination of bromhexine, chlorpheniramine, paracetamol, and pseudoephedrine in common cold medications (tablets and syrups). The separation of the analytes was achieved within 10 min, employing a mixture of 10 mM triethylamine-phosphoric acid buffer (pH 4.0) and MeOH (35:65, v/v) as isocratic mobile phase, pumped at 1.0 mL min−1 through a cyano column (5 µm particle size). The analytes were detected at 215 nm. Statistical experimental designs and graphic representations (response surface methodologies, Pareto charts) were used for selecting the proper detection wavelength, optimizing the mobile phase composition, and assessing method robustness. The linearity of the calibration (r > 0.99, n = 21) in the relevant ranges (up to 130% of the expected concentrations of the analytes in the formulations), method accuracy (bias < 2.0%), repeatability (RSD < 2.0%) and intermediate precision, were verified. In addition, specificity (peak purities with photodiode array detector >0.9997) and method robustness were evaluated, and system suitability parameters were determined. The validated method was successfully employed for the routine analysis of various commercial tablet and syrup pharmaceutical preparations against the common cold, showing satisfactory analyte recoveries and RSD values.
Fil: Vignaduzzo, Silvana Edit. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Kaufman, Teodoro Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina - Materia
-
Bromhexine
Chlorpheniramine
Experimental Design
Paracetamol
Validated HPLC determination
Pseudoephedrine - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/6078
Ver los metadatos del registro completo
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Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine FormulationsVignaduzzo, Silvana EditKaufman, Teodoro SaulBromhexineChlorpheniramineExperimental DesignParacetamolValidated HPLC determinationPseudoephedrinehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A simple and efficient liquid chromatographic method has been developed and validated for the simultaneous determination of bromhexine, chlorpheniramine, paracetamol, and pseudoephedrine in common cold medications (tablets and syrups). The separation of the analytes was achieved within 10 min, employing a mixture of 10 mM triethylamine-phosphoric acid buffer (pH 4.0) and MeOH (35:65, v/v) as isocratic mobile phase, pumped at 1.0 mL min−1 through a cyano column (5 µm particle size). The analytes were detected at 215 nm. Statistical experimental designs and graphic representations (response surface methodologies, Pareto charts) were used for selecting the proper detection wavelength, optimizing the mobile phase composition, and assessing method robustness. The linearity of the calibration (r > 0.99, n = 21) in the relevant ranges (up to 130% of the expected concentrations of the analytes in the formulations), method accuracy (bias < 2.0%), repeatability (RSD < 2.0%) and intermediate precision, were verified. In addition, specificity (peak purities with photodiode array detector >0.9997) and method robustness were evaluated, and system suitability parameters were determined. The validated method was successfully employed for the routine analysis of various commercial tablet and syrup pharmaceutical preparations against the common cold, showing satisfactory analyte recoveries and RSD values.Fil: Vignaduzzo, Silvana Edit. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Kaufman, Teodoro Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaTaylor & Francis2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6078Vignaduzzo, Silvana Edit; Kaufman, Teodoro Saul; Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations; Taylor & Francis; Journal of Liquid Chromatography and Related Technologies; 36; 20; 9-2013; 2829-28431082-6076enginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/10826076.2012.717055info:eu-repo/semantics/altIdentifier/doi/10.1080/10826076.2012.717055info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:04:00Zoai:ri.conicet.gov.ar:11336/6078instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:04:00.749CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations |
title |
Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations |
spellingShingle |
Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations Vignaduzzo, Silvana Edit Bromhexine Chlorpheniramine Experimental Design Paracetamol Validated HPLC determination Pseudoephedrine |
title_short |
Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations |
title_full |
Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations |
title_fullStr |
Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations |
title_full_unstemmed |
Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations |
title_sort |
Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations |
dc.creator.none.fl_str_mv |
Vignaduzzo, Silvana Edit Kaufman, Teodoro Saul |
author |
Vignaduzzo, Silvana Edit |
author_facet |
Vignaduzzo, Silvana Edit Kaufman, Teodoro Saul |
author_role |
author |
author2 |
Kaufman, Teodoro Saul |
author2_role |
author |
dc.subject.none.fl_str_mv |
Bromhexine Chlorpheniramine Experimental Design Paracetamol Validated HPLC determination Pseudoephedrine |
topic |
Bromhexine Chlorpheniramine Experimental Design Paracetamol Validated HPLC determination Pseudoephedrine |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A simple and efficient liquid chromatographic method has been developed and validated for the simultaneous determination of bromhexine, chlorpheniramine, paracetamol, and pseudoephedrine in common cold medications (tablets and syrups). The separation of the analytes was achieved within 10 min, employing a mixture of 10 mM triethylamine-phosphoric acid buffer (pH 4.0) and MeOH (35:65, v/v) as isocratic mobile phase, pumped at 1.0 mL min−1 through a cyano column (5 µm particle size). The analytes were detected at 215 nm. Statistical experimental designs and graphic representations (response surface methodologies, Pareto charts) were used for selecting the proper detection wavelength, optimizing the mobile phase composition, and assessing method robustness. The linearity of the calibration (r > 0.99, n = 21) in the relevant ranges (up to 130% of the expected concentrations of the analytes in the formulations), method accuracy (bias < 2.0%), repeatability (RSD < 2.0%) and intermediate precision, were verified. In addition, specificity (peak purities with photodiode array detector >0.9997) and method robustness were evaluated, and system suitability parameters were determined. The validated method was successfully employed for the routine analysis of various commercial tablet and syrup pharmaceutical preparations against the common cold, showing satisfactory analyte recoveries and RSD values. Fil: Vignaduzzo, Silvana Edit. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Kaufman, Teodoro Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina |
description |
A simple and efficient liquid chromatographic method has been developed and validated for the simultaneous determination of bromhexine, chlorpheniramine, paracetamol, and pseudoephedrine in common cold medications (tablets and syrups). The separation of the analytes was achieved within 10 min, employing a mixture of 10 mM triethylamine-phosphoric acid buffer (pH 4.0) and MeOH (35:65, v/v) as isocratic mobile phase, pumped at 1.0 mL min−1 through a cyano column (5 µm particle size). The analytes were detected at 215 nm. Statistical experimental designs and graphic representations (response surface methodologies, Pareto charts) were used for selecting the proper detection wavelength, optimizing the mobile phase composition, and assessing method robustness. The linearity of the calibration (r > 0.99, n = 21) in the relevant ranges (up to 130% of the expected concentrations of the analytes in the formulations), method accuracy (bias < 2.0%), repeatability (RSD < 2.0%) and intermediate precision, were verified. In addition, specificity (peak purities with photodiode array detector >0.9997) and method robustness were evaluated, and system suitability parameters were determined. The validated method was successfully employed for the routine analysis of various commercial tablet and syrup pharmaceutical preparations against the common cold, showing satisfactory analyte recoveries and RSD values. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/6078 Vignaduzzo, Silvana Edit; Kaufman, Teodoro Saul; Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations; Taylor & Francis; Journal of Liquid Chromatography and Related Technologies; 36; 20; 9-2013; 2829-2843 1082-6076 |
url |
http://hdl.handle.net/11336/6078 |
identifier_str_mv |
Vignaduzzo, Silvana Edit; Kaufman, Teodoro Saul; Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations; Taylor & Francis; Journal of Liquid Chromatography and Related Technologies; 36; 20; 9-2013; 2829-2843 1082-6076 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/10826076.2012.717055 info:eu-repo/semantics/altIdentifier/doi/10.1080/10826076.2012.717055 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083182928068608 |
score |
13.22299 |