Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam

Autores
Otarola, Jessica Johana; Lista, Adriana Guillermina; Fernández Band, Beatriz Susana; Garrido, Mariano Enrique
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A simple and fast capillary electrophoresis method has been developed to determine the amount of piroxicam loaded in a drug delivery system based on nanostructured lipid carriers (NLC). The entrapment efficiency of the nanostructured lipid carrier was estimated by measuring the concentration of drug not entrapped in a suspension of NLC. The influence of different parameters on migration times, peak symmetry, efficiency and resolution was studied; these parameters included the pH of the electrophoretic buffer solution and the applied voltage. The piroxicam peak was obtained with a satisfactory resolution. The separation was carried out using a running buffer, which it is composed of 50 mmol L-1 ammonium acetate and 13.75 mmol L-1 ammonia at pH 9. The optimal voltage was 20kV and the cartridge temperature was 20°C. The corresponding calibration curve was linear over the range of 2.7 ? 5.4 µg mL-1 of NLC suspension. The reproducibility of migration time and peak area were investigated, and the obtained RSD% values (n = 5) were 0.99 and 2.13, respectively.
Fil: Otarola, Jessica Johana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina
Fil: Lista, Adriana Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina
Fil: Fernández Band, Beatriz Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina
Fil: Garrido, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina
Materia
CAPILLARY ELECTROPHORESIS
DRUG DELIVERY SYSTEM
NANOSTRUCTURED LIPID CARRIER
PIROXICAM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4710
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicamOtarola, Jessica JohanaLista, Adriana GuillerminaFernández Band, Beatriz SusanaGarrido, Mariano EnriqueCAPILLARY ELECTROPHORESISDRUG DELIVERY SYSTEMNANOSTRUCTURED LIPID CARRIERPIROXICAMhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A simple and fast capillary electrophoresis method has been developed to determine the amount of piroxicam loaded in a drug delivery system based on nanostructured lipid carriers (NLC). The entrapment efficiency of the nanostructured lipid carrier was estimated by measuring the concentration of drug not entrapped in a suspension of NLC. The influence of different parameters on migration times, peak symmetry, efficiency and resolution was studied; these parameters included the pH of the electrophoretic buffer solution and the applied voltage. The piroxicam peak was obtained with a satisfactory resolution. The separation was carried out using a running buffer, which it is composed of 50 mmol L-1 ammonium acetate and 13.75 mmol L-1 ammonia at pH 9. The optimal voltage was 20kV and the cartridge temperature was 20°C. The corresponding calibration curve was linear over the range of 2.7 ? 5.4 µg mL-1 of NLC suspension. The reproducibility of migration time and peak area were investigated, and the obtained RSD% values (n = 5) were 0.99 and 2.13, respectively.Fil: Otarola, Jessica Johana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; ArgentinaFil: Lista, Adriana Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; ArgentinaFil: Fernández Band, Beatriz Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; ArgentinaFil: Garrido, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; ArgentinaElsevier2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4710Otarola, Jessica Johana; Lista, Adriana Guillermina; Fernández Band, Beatriz Susana; Garrido, Mariano Enrique; Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam; Elsevier; Journal of Pharmaceutical Analysis; 5; 1; 1-2015; 70-732095-1779enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jpha.2014.05.003info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2095177914000446info:eu-repo/semantics/altIdentifier/issn/2095-1779info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:01:06Zoai:ri.conicet.gov.ar:11336/4710instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:01:06.385CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam
title Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam
spellingShingle Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam
Otarola, Jessica Johana
CAPILLARY ELECTROPHORESIS
DRUG DELIVERY SYSTEM
NANOSTRUCTURED LIPID CARRIER
PIROXICAM
title_short Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam
title_full Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam
title_fullStr Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam
title_full_unstemmed Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam
title_sort Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam
dc.creator.none.fl_str_mv Otarola, Jessica Johana
Lista, Adriana Guillermina
Fernández Band, Beatriz Susana
Garrido, Mariano Enrique
author Otarola, Jessica Johana
author_facet Otarola, Jessica Johana
Lista, Adriana Guillermina
Fernández Band, Beatriz Susana
Garrido, Mariano Enrique
author_role author
author2 Lista, Adriana Guillermina
Fernández Band, Beatriz Susana
Garrido, Mariano Enrique
author2_role author
author
author
dc.subject.none.fl_str_mv CAPILLARY ELECTROPHORESIS
DRUG DELIVERY SYSTEM
NANOSTRUCTURED LIPID CARRIER
PIROXICAM
topic CAPILLARY ELECTROPHORESIS
DRUG DELIVERY SYSTEM
NANOSTRUCTURED LIPID CARRIER
PIROXICAM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A simple and fast capillary electrophoresis method has been developed to determine the amount of piroxicam loaded in a drug delivery system based on nanostructured lipid carriers (NLC). The entrapment efficiency of the nanostructured lipid carrier was estimated by measuring the concentration of drug not entrapped in a suspension of NLC. The influence of different parameters on migration times, peak symmetry, efficiency and resolution was studied; these parameters included the pH of the electrophoretic buffer solution and the applied voltage. The piroxicam peak was obtained with a satisfactory resolution. The separation was carried out using a running buffer, which it is composed of 50 mmol L-1 ammonium acetate and 13.75 mmol L-1 ammonia at pH 9. The optimal voltage was 20kV and the cartridge temperature was 20°C. The corresponding calibration curve was linear over the range of 2.7 ? 5.4 µg mL-1 of NLC suspension. The reproducibility of migration time and peak area were investigated, and the obtained RSD% values (n = 5) were 0.99 and 2.13, respectively.
Fil: Otarola, Jessica Johana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina
Fil: Lista, Adriana Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina
Fil: Fernández Band, Beatriz Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina
Fil: Garrido, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina
description A simple and fast capillary electrophoresis method has been developed to determine the amount of piroxicam loaded in a drug delivery system based on nanostructured lipid carriers (NLC). The entrapment efficiency of the nanostructured lipid carrier was estimated by measuring the concentration of drug not entrapped in a suspension of NLC. The influence of different parameters on migration times, peak symmetry, efficiency and resolution was studied; these parameters included the pH of the electrophoretic buffer solution and the applied voltage. The piroxicam peak was obtained with a satisfactory resolution. The separation was carried out using a running buffer, which it is composed of 50 mmol L-1 ammonium acetate and 13.75 mmol L-1 ammonia at pH 9. The optimal voltage was 20kV and the cartridge temperature was 20°C. The corresponding calibration curve was linear over the range of 2.7 ? 5.4 µg mL-1 of NLC suspension. The reproducibility of migration time and peak area were investigated, and the obtained RSD% values (n = 5) were 0.99 and 2.13, respectively.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4710
Otarola, Jessica Johana; Lista, Adriana Guillermina; Fernández Band, Beatriz Susana; Garrido, Mariano Enrique; Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam; Elsevier; Journal of Pharmaceutical Analysis; 5; 1; 1-2015; 70-73
2095-1779
url http://hdl.handle.net/11336/4710
identifier_str_mv Otarola, Jessica Johana; Lista, Adriana Guillermina; Fernández Band, Beatriz Susana; Garrido, Mariano Enrique; Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam; Elsevier; Journal of Pharmaceutical Analysis; 5; 1; 1-2015; 70-73
2095-1779
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jpha.2014.05.003
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2095177914000446
info:eu-repo/semantics/altIdentifier/issn/2095-1779
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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