Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholestero...

Autores
Rashidi, Khodabakhsh; Mahmoudi, Majid; Mohammadi, Ghobad; Zangeneh, Mohammad Mahdi; Korani, Shahla; Goicoechea, Hector Casimiro; Gu, Hui-Wen; Jalalvand, Ali R.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, we have fabricated a novel amperometric cholesterol (CHO) biosensor because of the importance of determination of CHO levels in blood which is an important parameter for diagnosis and prevention of disease. To achieve this goal, cholesterol oxidase, cholesterol esterase and horseradish peroxidase were simultaneously co-immobilized onto a glassy carbon electrode (GCE) modified with gold nanoparticles/chitin-ionic liquid/poly(3,4-ethylenedioxypyrrole)/graphene-multiwalled carbon nanotubes-1,1′-ferrocenedicarboxylic acid-ionic liquid. Modifications applied to the bare GCE were characterized by cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The biosensor detected CHO in linear ranges of 0.1–25 μM and 25–950 μM with a detection limit of 0.07 μM. The sensitivity of the biosensor was estimated to be 6.6 μA μM−1 cm−2, its response time was <5 s and Michaelis-Menten constant was calculated to be 0.12 μM. Results obtained in this study revealed that the biosensor was selective, sensitive, stable, repeatable and reproducible. Finally, the biosensor was successfully applied to the determination of CHO levels in rats plasma.
Fil: Rashidi, Khodabakhsh. Kermanshah University of Medical Sciences; Irán
Fil: Mahmoudi, Majid. Kermanshah University of Medical Sciences; Irán
Fil: Mohammadi, Ghobad. Kermanshah University of Medical Sciences; Irán
Fil: Zangeneh, Mohammad Mahdi. Kermanshah University of Medical Sciences; Irán. Razi University,; Irán
Fil: Korani, Shahla. Kermanshah University of Medical Sciences; Irán
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Gu, Hui-Wen. Yangtze University; China
Fil: Jalalvand, Ali R.. Kermanshah University of Medical Sciences; Irán
Materia
BIOSENSOR
CHOLESTEROL
RATS PLASMA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84761
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/84761
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterolRashidi, KhodabakhshMahmoudi, MajidMohammadi, GhobadZangeneh, Mohammad MahdiKorani, ShahlaGoicoechea, Hector CasimiroGu, Hui-WenJalalvand, Ali R.BIOSENSORCHOLESTEROLRATS PLASMAhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work, we have fabricated a novel amperometric cholesterol (CHO) biosensor because of the importance of determination of CHO levels in blood which is an important parameter for diagnosis and prevention of disease. To achieve this goal, cholesterol oxidase, cholesterol esterase and horseradish peroxidase were simultaneously co-immobilized onto a glassy carbon electrode (GCE) modified with gold nanoparticles/chitin-ionic liquid/poly(3,4-ethylenedioxypyrrole)/graphene-multiwalled carbon nanotubes-1,1′-ferrocenedicarboxylic acid-ionic liquid. Modifications applied to the bare GCE were characterized by cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The biosensor detected CHO in linear ranges of 0.1–25 μM and 25–950 μM with a detection limit of 0.07 μM. The sensitivity of the biosensor was estimated to be 6.6 μA μM−1 cm−2, its response time was <5 s and Michaelis-Menten constant was calculated to be 0.12 μM. Results obtained in this study revealed that the biosensor was selective, sensitive, stable, repeatable and reproducible. Finally, the biosensor was successfully applied to the determination of CHO levels in rats plasma.Fil: Rashidi, Khodabakhsh. Kermanshah University of Medical Sciences; IránFil: Mahmoudi, Majid. Kermanshah University of Medical Sciences; IránFil: Mohammadi, Ghobad. Kermanshah University of Medical Sciences; IránFil: Zangeneh, Mohammad Mahdi. Kermanshah University of Medical Sciences; Irán. Razi University,; IránFil: Korani, Shahla. Kermanshah University of Medical Sciences; IránFil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Gu, Hui-Wen. Yangtze University; ChinaFil: Jalalvand, Ali R.. Kermanshah University of Medical Sciences; IránElsevier Science2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84761Rashidi, Khodabakhsh; Mahmoudi, Majid; Mohammadi, Ghobad; Zangeneh, Mohammad Mahdi; Korani, Shahla; et al.; Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol; Elsevier Science; International Journal of Biological Macromolecules; 120; Part A; 12-2018; 587-5950141-8130CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141813018330186info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2018.08.163info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:24:17Zoai:ri.conicet.gov.ar:11336/84761instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:24:18.168CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol
title Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol
spellingShingle Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol
Rashidi, Khodabakhsh
BIOSENSOR
CHOLESTEROL
RATS PLASMA
title_short Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol
title_full Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol
title_fullStr Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol
title_full_unstemmed Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol
title_sort Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol
dc.creator.none.fl_str_mv Rashidi, Khodabakhsh
Mahmoudi, Majid
Mohammadi, Ghobad
Zangeneh, Mohammad Mahdi
Korani, Shahla
Goicoechea, Hector Casimiro
Gu, Hui-Wen
Jalalvand, Ali R.
author Rashidi, Khodabakhsh
author_facet Rashidi, Khodabakhsh
Mahmoudi, Majid
Mohammadi, Ghobad
Zangeneh, Mohammad Mahdi
Korani, Shahla
Goicoechea, Hector Casimiro
Gu, Hui-Wen
Jalalvand, Ali R.
author_role author
author2 Mahmoudi, Majid
Mohammadi, Ghobad
Zangeneh, Mohammad Mahdi
Korani, Shahla
Goicoechea, Hector Casimiro
Gu, Hui-Wen
Jalalvand, Ali R.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOSENSOR
CHOLESTEROL
RATS PLASMA
topic BIOSENSOR
CHOLESTEROL
RATS PLASMA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work, we have fabricated a novel amperometric cholesterol (CHO) biosensor because of the importance of determination of CHO levels in blood which is an important parameter for diagnosis and prevention of disease. To achieve this goal, cholesterol oxidase, cholesterol esterase and horseradish peroxidase were simultaneously co-immobilized onto a glassy carbon electrode (GCE) modified with gold nanoparticles/chitin-ionic liquid/poly(3,4-ethylenedioxypyrrole)/graphene-multiwalled carbon nanotubes-1,1′-ferrocenedicarboxylic acid-ionic liquid. Modifications applied to the bare GCE were characterized by cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The biosensor detected CHO in linear ranges of 0.1–25 μM and 25–950 μM with a detection limit of 0.07 μM. The sensitivity of the biosensor was estimated to be 6.6 μA μM−1 cm−2, its response time was <5 s and Michaelis-Menten constant was calculated to be 0.12 μM. Results obtained in this study revealed that the biosensor was selective, sensitive, stable, repeatable and reproducible. Finally, the biosensor was successfully applied to the determination of CHO levels in rats plasma.
Fil: Rashidi, Khodabakhsh. Kermanshah University of Medical Sciences; Irán
Fil: Mahmoudi, Majid. Kermanshah University of Medical Sciences; Irán
Fil: Mohammadi, Ghobad. Kermanshah University of Medical Sciences; Irán
Fil: Zangeneh, Mohammad Mahdi. Kermanshah University of Medical Sciences; Irán. Razi University,; Irán
Fil: Korani, Shahla. Kermanshah University of Medical Sciences; Irán
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Gu, Hui-Wen. Yangtze University; China
Fil: Jalalvand, Ali R.. Kermanshah University of Medical Sciences; Irán
description In this work, we have fabricated a novel amperometric cholesterol (CHO) biosensor because of the importance of determination of CHO levels in blood which is an important parameter for diagnosis and prevention of disease. To achieve this goal, cholesterol oxidase, cholesterol esterase and horseradish peroxidase were simultaneously co-immobilized onto a glassy carbon electrode (GCE) modified with gold nanoparticles/chitin-ionic liquid/poly(3,4-ethylenedioxypyrrole)/graphene-multiwalled carbon nanotubes-1,1′-ferrocenedicarboxylic acid-ionic liquid. Modifications applied to the bare GCE were characterized by cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The biosensor detected CHO in linear ranges of 0.1–25 μM and 25–950 μM with a detection limit of 0.07 μM. The sensitivity of the biosensor was estimated to be 6.6 μA μM−1 cm−2, its response time was <5 s and Michaelis-Menten constant was calculated to be 0.12 μM. Results obtained in this study revealed that the biosensor was selective, sensitive, stable, repeatable and reproducible. Finally, the biosensor was successfully applied to the determination of CHO levels in rats plasma.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84761
Rashidi, Khodabakhsh; Mahmoudi, Majid; Mohammadi, Ghobad; Zangeneh, Mohammad Mahdi; Korani, Shahla; et al.; Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol; Elsevier Science; International Journal of Biological Macromolecules; 120; Part A; 12-2018; 587-595
0141-8130
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84761
identifier_str_mv Rashidi, Khodabakhsh; Mahmoudi, Majid; Mohammadi, Ghobad; Zangeneh, Mohammad Mahdi; Korani, Shahla; et al.; Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol; Elsevier Science; International Journal of Biological Macromolecules; 120; Part A; 12-2018; 587-595
0141-8130
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141813018330186
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2018.08.163
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981346796896256
score 12.993085

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