Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant

Autores
Kotik, D.; Sansberro, Pedro Alfonso; Luna, Claudia Verónica
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adventitious bud regeneration was achieved from hypocotyls, cotyledons and leaf explants of Achyrocline satureioides. Organogenesis was induced from every explant cultured on Murashige and Skoog semisolid medium (plus sucrose 30g·L-1) containing different combinations of 6-benzyladenine (BA) and alpha-naphtalenacetic acid (NAA) under 116 micromol·m-2·s-1 photosynthetic photon flux density (PPFD), photoperiod 14 h and at 27±2ºC. The regeneration was similar for every tested explant and varied between 64 and 83%. The number of buds formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In order to stimulate In vitro rooting, regenerative leaves were sub cultured from the best induction medium in MS lacking plant growth regulators for the same periods. Every plantlet raised In vitro was phenotypically normal and successfully hardened to ex vitro conditions. An experimental field plot with 60-day-old in vitro regenerated plants was established.
Fil: Kotik, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Materia
ACHYROCLINE
MICROPROPAGATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5718
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plantKotik, D.Sansberro, Pedro AlfonsoLuna, Claudia VerónicaACHYROCLINEMICROPROPAGATIONhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Adventitious bud regeneration was achieved from hypocotyls, cotyledons and leaf explants of Achyrocline satureioides. Organogenesis was induced from every explant cultured on Murashige and Skoog semisolid medium (plus sucrose 30g·L-1) containing different combinations of 6-benzyladenine (BA) and alpha-naphtalenacetic acid (NAA) under 116 micromol·m-2·s-1 photosynthetic photon flux density (PPFD), photoperiod 14 h and at 27±2ºC. The regeneration was similar for every tested explant and varied between 64 and 83%. The number of buds formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In order to stimulate In vitro rooting, regenerative leaves were sub cultured from the best induction medium in MS lacking plant growth regulators for the same periods. Every plantlet raised In vitro was phenotypically normal and successfully hardened to ex vitro conditions. An experimental field plot with 60-day-old in vitro regenerated plants was established.Fil: Kotik, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaScienceDomain International2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5718Kotik, D.; Sansberro, Pedro Alfonso; Luna, Claudia Verónica; Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant; ScienceDomain International; European Journal of Medicinal Plants; 4; 10; 5-2014; 1200-12092231-0894enginfo:eu-repo/semantics/altIdentifier/url/http://sciencedomain.org/abstract/5135info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.9734/EJMP/2014/8800info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:31Zoai:ri.conicet.gov.ar:11336/5718instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:31.786CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant
title Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant
spellingShingle Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant
Kotik, D.
ACHYROCLINE
MICROPROPAGATION
title_short Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant
title_full Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant
title_fullStr Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant
title_full_unstemmed Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant
title_sort Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant
dc.creator.none.fl_str_mv Kotik, D.
Sansberro, Pedro Alfonso
Luna, Claudia Verónica
author Kotik, D.
author_facet Kotik, D.
Sansberro, Pedro Alfonso
Luna, Claudia Verónica
author_role author
author2 Sansberro, Pedro Alfonso
Luna, Claudia Verónica
author2_role author
author
dc.subject.none.fl_str_mv ACHYROCLINE
MICROPROPAGATION
topic ACHYROCLINE
MICROPROPAGATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Adventitious bud regeneration was achieved from hypocotyls, cotyledons and leaf explants of Achyrocline satureioides. Organogenesis was induced from every explant cultured on Murashige and Skoog semisolid medium (plus sucrose 30g·L-1) containing different combinations of 6-benzyladenine (BA) and alpha-naphtalenacetic acid (NAA) under 116 micromol·m-2·s-1 photosynthetic photon flux density (PPFD), photoperiod 14 h and at 27±2ºC. The regeneration was similar for every tested explant and varied between 64 and 83%. The number of buds formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In order to stimulate In vitro rooting, regenerative leaves were sub cultured from the best induction medium in MS lacking plant growth regulators for the same periods. Every plantlet raised In vitro was phenotypically normal and successfully hardened to ex vitro conditions. An experimental field plot with 60-day-old in vitro regenerated plants was established.
Fil: Kotik, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
description Adventitious bud regeneration was achieved from hypocotyls, cotyledons and leaf explants of Achyrocline satureioides. Organogenesis was induced from every explant cultured on Murashige and Skoog semisolid medium (plus sucrose 30g·L-1) containing different combinations of 6-benzyladenine (BA) and alpha-naphtalenacetic acid (NAA) under 116 micromol·m-2·s-1 photosynthetic photon flux density (PPFD), photoperiod 14 h and at 27±2ºC. The regeneration was similar for every tested explant and varied between 64 and 83%. The number of buds formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In order to stimulate In vitro rooting, regenerative leaves were sub cultured from the best induction medium in MS lacking plant growth regulators for the same periods. Every plantlet raised In vitro was phenotypically normal and successfully hardened to ex vitro conditions. An experimental field plot with 60-day-old in vitro regenerated plants was established.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5718
Kotik, D.; Sansberro, Pedro Alfonso; Luna, Claudia Verónica; Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant; ScienceDomain International; European Journal of Medicinal Plants; 4; 10; 5-2014; 1200-1209
2231-0894
url http://hdl.handle.net/11336/5718
identifier_str_mv Kotik, D.; Sansberro, Pedro Alfonso; Luna, Claudia Verónica; Adventitious bud formation and plantlet regeneration of Achyrocline satureoides-A multipurpose medicinal plant; ScienceDomain International; European Journal of Medicinal Plants; 4; 10; 5-2014; 1200-1209
2231-0894
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://sciencedomain.org/abstract/5135
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.9734/EJMP/2014/8800
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv ScienceDomain International
publisher.none.fl_str_mv ScienceDomain International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269231042789376
score 13.13397

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