Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in mice

Autores
Bertoldi, María Laura; Luque, E. M.; Carlini, Valeria Paola; Vincenti, Laura María; Stutz, Graciela; Santillán, M.E.; Ruiz, R. D.; Fiol de Cuneo, Marta; Martini, Ana Carolina
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ghrelin (Ghr) is a gut/hypothalamus peptide with inhibitory actions on reproductive physiology; however, there are no previous reports of its role on estrous behavior. Under the hypothesis that the increase of plasma Ghr during food restriction (FR) is responsible for receptivity reduction, we intended to evaluate the receptivity percentage of female mice subjected to: exp. 1) acute and chronic FR and Ghr administration (3 nmol/animal/day, s. c.) and exp. 2) the co-administration of a ghrelin antagonist [ant=(d-Lys3)-GHRP-6; 6 nmol/animal/day s. c.]. All females were ovariectomized, primed with steroids, trained, and randomly subjected every week to each one of several protocols, followed by a behavioral test. Experiment 1 (n=8): basal, no treatment; acute FR (aFR), 24-h fasting; chronic FR (cFR), 50% FR for 5 days; acute ghrelin (aGhr), Ghr 30 min before test and chronic ghrelin (cGhr), Ghr for 5 days. Except for cGhr, all treatments significantly decreased the percentage of receptivity (mean±SEM): basal 61.9±6.0, aFR 33.1±8.1, cFR 18.8±7.7, aGhr 45.6±10.6, p<0.05 vs. basal. In exp. 2 (n=11), except for cFR+ant (55.0±6.4) the co-administration of the antagonist reversed the deleterious effects detected in exp. 1: basal 70.9±5.4; aFR+ant 72.3±7.6; aGhr+ant 73.6±4.7. As expected, the administration of vehicle or antagonist alone did not modify receptivity. Besides, we found a significant correlation between percentage of body weight loss and percentage of receptivity reduction (r=0.62, p=0.0004). This is the first study demonstrating that ghrelin is able to inhibit female mice sexual behavior and that is involved, at least in part, in receptivity reduction after food scarcity.
Fil: Bertoldi, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Luque, E. M.. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;
Fil: Carlini, Valeria Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Vincenti, Laura María. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;
Fil: Stutz, Graciela. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;
Fil: Santillán, M.E.. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;
Fil: Ruiz, R. D.. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;
Fil: Fiol de Cuneo, Marta. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;
Fil: Martini, Ana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Materia
GRHELIN ANTAGONIST
(D-LYS3)-GHRP6
SEXUAL BEHAVIOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230825
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in miceBertoldi, María LauraLuque, E. M.Carlini, Valeria PaolaVincenti, Laura MaríaStutz, GracielaSantillán, M.E.Ruiz, R. D.Fiol de Cuneo, MartaMartini, Ana CarolinaGRHELIN ANTAGONIST(D-LYS3)-GHRP6SEXUAL BEHAVIORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ghrelin (Ghr) is a gut/hypothalamus peptide with inhibitory actions on reproductive physiology; however, there are no previous reports of its role on estrous behavior. Under the hypothesis that the increase of plasma Ghr during food restriction (FR) is responsible for receptivity reduction, we intended to evaluate the receptivity percentage of female mice subjected to: exp. 1) acute and chronic FR and Ghr administration (3 nmol/animal/day, s. c.) and exp. 2) the co-administration of a ghrelin antagonist [ant=(d-Lys3)-GHRP-6; 6 nmol/animal/day s. c.]. All females were ovariectomized, primed with steroids, trained, and randomly subjected every week to each one of several protocols, followed by a behavioral test. Experiment 1 (n=8): basal, no treatment; acute FR (aFR), 24-h fasting; chronic FR (cFR), 50% FR for 5 days; acute ghrelin (aGhr), Ghr 30 min before test and chronic ghrelin (cGhr), Ghr for 5 days. Except for cGhr, all treatments significantly decreased the percentage of receptivity (mean±SEM): basal 61.9±6.0, aFR 33.1±8.1, cFR 18.8±7.7, aGhr 45.6±10.6, p<0.05 vs. basal. In exp. 2 (n=11), except for cFR+ant (55.0±6.4) the co-administration of the antagonist reversed the deleterious effects detected in exp. 1: basal 70.9±5.4; aFR+ant 72.3±7.6; aGhr+ant 73.6±4.7. As expected, the administration of vehicle or antagonist alone did not modify receptivity. Besides, we found a significant correlation between percentage of body weight loss and percentage of receptivity reduction (r=0.62, p=0.0004). This is the first study demonstrating that ghrelin is able to inhibit female mice sexual behavior and that is involved, at least in part, in receptivity reduction after food scarcity.Fil: Bertoldi, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Luque, E. M.. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;Fil: Carlini, Valeria Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Vincenti, Laura María. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;Fil: Stutz, Graciela. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;Fil: Santillán, M.E.. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;Fil: Ruiz, R. D.. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;Fil: Fiol de Cuneo, Marta. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;Fil: Martini, Ana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaGeorg Thieme Verlagiaorl@iaorl.org2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230825Bertoldi, María Laura; Luque, E. M.; Carlini, Valeria Paola; Vincenti, Laura María; Stutz, Graciela; et al.; Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in mice; Georg Thieme Verlagiaorl@iaorl.org; Hormone and Metabolic Research; 43; 7; 4-2011; 494-4990018-50431439-4286CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.thieme-connect.com/DOI/DOI?10.1055/s-0031-1277228info:eu-repo/semantics/altIdentifier/doi/10.1055/s-0031-1277228info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:15:12Zoai:ri.conicet.gov.ar:11336/230825instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:15:12.476CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in mice
title Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in mice
spellingShingle Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in mice
Bertoldi, María Laura
GRHELIN ANTAGONIST
(D-LYS3)-GHRP6
SEXUAL BEHAVIOR
title_short Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in mice
title_full Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in mice
title_fullStr Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in mice
title_full_unstemmed Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in mice
title_sort Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in mice
dc.creator.none.fl_str_mv Bertoldi, María Laura
Luque, E. M.
Carlini, Valeria Paola
Vincenti, Laura María
Stutz, Graciela
Santillán, M.E.
Ruiz, R. D.
Fiol de Cuneo, Marta
Martini, Ana Carolina
author Bertoldi, María Laura
author_facet Bertoldi, María Laura
Luque, E. M.
Carlini, Valeria Paola
Vincenti, Laura María
Stutz, Graciela
Santillán, M.E.
Ruiz, R. D.
Fiol de Cuneo, Marta
Martini, Ana Carolina
author_role author
author2 Luque, E. M.
Carlini, Valeria Paola
Vincenti, Laura María
Stutz, Graciela
Santillán, M.E.
Ruiz, R. D.
Fiol de Cuneo, Marta
Martini, Ana Carolina
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GRHELIN ANTAGONIST
(D-LYS3)-GHRP6
SEXUAL BEHAVIOR
topic GRHELIN ANTAGONIST
(D-LYS3)-GHRP6
SEXUAL BEHAVIOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Ghrelin (Ghr) is a gut/hypothalamus peptide with inhibitory actions on reproductive physiology; however, there are no previous reports of its role on estrous behavior. Under the hypothesis that the increase of plasma Ghr during food restriction (FR) is responsible for receptivity reduction, we intended to evaluate the receptivity percentage of female mice subjected to: exp. 1) acute and chronic FR and Ghr administration (3 nmol/animal/day, s. c.) and exp. 2) the co-administration of a ghrelin antagonist [ant=(d-Lys3)-GHRP-6; 6 nmol/animal/day s. c.]. All females were ovariectomized, primed with steroids, trained, and randomly subjected every week to each one of several protocols, followed by a behavioral test. Experiment 1 (n=8): basal, no treatment; acute FR (aFR), 24-h fasting; chronic FR (cFR), 50% FR for 5 days; acute ghrelin (aGhr), Ghr 30 min before test and chronic ghrelin (cGhr), Ghr for 5 days. Except for cGhr, all treatments significantly decreased the percentage of receptivity (mean±SEM): basal 61.9±6.0, aFR 33.1±8.1, cFR 18.8±7.7, aGhr 45.6±10.6, p<0.05 vs. basal. In exp. 2 (n=11), except for cFR+ant (55.0±6.4) the co-administration of the antagonist reversed the deleterious effects detected in exp. 1: basal 70.9±5.4; aFR+ant 72.3±7.6; aGhr+ant 73.6±4.7. As expected, the administration of vehicle or antagonist alone did not modify receptivity. Besides, we found a significant correlation between percentage of body weight loss and percentage of receptivity reduction (r=0.62, p=0.0004). This is the first study demonstrating that ghrelin is able to inhibit female mice sexual behavior and that is involved, at least in part, in receptivity reduction after food scarcity.
Fil: Bertoldi, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Luque, E. M.. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;
Fil: Carlini, Valeria Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Vincenti, Laura María. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;
Fil: Stutz, Graciela. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;
Fil: Santillán, M.E.. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;
Fil: Ruiz, R. D.. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;
Fil: Fiol de Cuneo, Marta. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Instituto de Fisiologia Profesor Oscar Orias;
Fil: Martini, Ana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
description Ghrelin (Ghr) is a gut/hypothalamus peptide with inhibitory actions on reproductive physiology; however, there are no previous reports of its role on estrous behavior. Under the hypothesis that the increase of plasma Ghr during food restriction (FR) is responsible for receptivity reduction, we intended to evaluate the receptivity percentage of female mice subjected to: exp. 1) acute and chronic FR and Ghr administration (3 nmol/animal/day, s. c.) and exp. 2) the co-administration of a ghrelin antagonist [ant=(d-Lys3)-GHRP-6; 6 nmol/animal/day s. c.]. All females were ovariectomized, primed with steroids, trained, and randomly subjected every week to each one of several protocols, followed by a behavioral test. Experiment 1 (n=8): basal, no treatment; acute FR (aFR), 24-h fasting; chronic FR (cFR), 50% FR for 5 days; acute ghrelin (aGhr), Ghr 30 min before test and chronic ghrelin (cGhr), Ghr for 5 days. Except for cGhr, all treatments significantly decreased the percentage of receptivity (mean±SEM): basal 61.9±6.0, aFR 33.1±8.1, cFR 18.8±7.7, aGhr 45.6±10.6, p<0.05 vs. basal. In exp. 2 (n=11), except for cFR+ant (55.0±6.4) the co-administration of the antagonist reversed the deleterious effects detected in exp. 1: basal 70.9±5.4; aFR+ant 72.3±7.6; aGhr+ant 73.6±4.7. As expected, the administration of vehicle or antagonist alone did not modify receptivity. Besides, we found a significant correlation between percentage of body weight loss and percentage of receptivity reduction (r=0.62, p=0.0004). This is the first study demonstrating that ghrelin is able to inhibit female mice sexual behavior and that is involved, at least in part, in receptivity reduction after food scarcity.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230825
Bertoldi, María Laura; Luque, E. M.; Carlini, Valeria Paola; Vincenti, Laura María; Stutz, Graciela; et al.; Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in mice; Georg Thieme Verlagiaorl@iaorl.org; Hormone and Metabolic Research; 43; 7; 4-2011; 494-499
0018-5043
1439-4286
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230825
identifier_str_mv Bertoldi, María Laura; Luque, E. M.; Carlini, Valeria Paola; Vincenti, Laura María; Stutz, Graciela; et al.; Inhibitory effects of ghrelin on sexual behavior: Role of the peptide in the receptivity reduction induced by food restriction in mice; Georg Thieme Verlagiaorl@iaorl.org; Hormone and Metabolic Research; 43; 7; 4-2011; 494-499
0018-5043
1439-4286
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.thieme-connect.com/DOI/DOI?10.1055/s-0031-1277228
info:eu-repo/semantics/altIdentifier/doi/10.1055/s-0031-1277228
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Georg Thieme Verlagiaorl@iaorl.org
publisher.none.fl_str_mv Georg Thieme Verlagiaorl@iaorl.org
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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