Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy
- Autores
- Garcés, Maria Fernanda; Buell Acosta, Julieth Daniela; Ángel Müller, Edith; Parada Baños, Arturo José; Acosta Alvarez, Jaidy; Saavedra López, Harold Felipe; Franco Vega, Roberto; Maldonado Acosta, Luis Miguel; Escobar Cordoba, Franklin; Vásquez Romero, Keydy; Lacunza, Ezequiel; Caminos Cepeda, Sofía Alexandra; Nogueiras, Rubén; Diéguez, Carlos; Ruiz Parra, Ariel Iván; Caminos, Jorge Eduardo
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The Liver-Expressed Antimicrobial Peptide 2 (LEAP-2) has emerged as an endogenous GHS-R antagonist and blunts the orexigenic action of ghrelin. This study aimed to determine the Ghrelin/LEAP-2 ratio in humans and rats during pregnancy. In humans, we conducted a nested case-control study within an observational prospective cohort. Healthy and mild preeclamptic pregnant women were studied at each trimester of gestation and three months postpartum. In addition, a group of non-pregnant women was studied into the follicular and luteal phases of the menstrual cycle. Furthermore, Ghrelin/LEAP-2 ratio was investigated in non-pregnant rats and at different periods of rat pregnancy. Human and rat serum ghrelin and LEAP-2 levels were determined using the commercially available ELISA kits. The Ghrelin/LEAP-2 ratio peak around the second trimester of gestation in healthy pregnant women (p < 0.05). Additionally, there were no statistically significant differences in Ghrelin/LEAP-2 ratio between healthy and preeclamptic pregnant women at each trimester of gestation (p > 0.05). The Ghrelin/LEAP-2 ratio in pregnant rat reached the peak around mid-gestation with a similar pattern to the human pregnancy. LEAP-2 was visualized by immunohistochemistry in human term placenta and rat placentas on days 12, 16 and 21 of pregnancy. In conclusion, this study provides the first evidence of a Ghrelin/LEAP-2 ratio peak around the half-way point of pregnancy onwards during human and rat pregnancy, and it might be associated with increased rates of weight gain during pregnancy. Thus, this study suggests that LEAP-2 and Ghrelin/LEAP-2 ratio might play an important role in maternal physiology adaptation of weight gain during pregnancy.
Fil: Garcés, Maria Fernanda. Universidad Nacional de Colombia; Colombia
Fil: Buell Acosta, Julieth Daniela. Universidad Nacional de Colombia; Colombia
Fil: Ángel Müller, Edith. Universidad Nacional de Colombia; Colombia
Fil: Parada Baños, Arturo José. Universidad Nacional de Colombia; Colombia
Fil: Acosta Alvarez, Jaidy. Universidad Nacional de Colombia; Colombia
Fil: Saavedra López, Harold Felipe. Universidad Nacional de Colombia; Colombia
Fil: Franco Vega, Roberto. Universidad Nacional de Colombia; Colombia
Fil: Maldonado Acosta, Luis Miguel. Universidad Nacional de Colombia; Colombia
Fil: Escobar Cordoba, Franklin. Universidad Nacional de Colombia; Colombia
Fil: Vásquez Romero, Keydy. Universidad de los Andes; Colombia
Fil: Lacunza, Ezequiel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Caminos Cepeda, Sofía Alexandra. Universitat Pompeu Fabra; España
Fil: Nogueiras, Rubén. Universidad de Santiago de Compostela; España
Fil: Diéguez, Carlos. Universidad de Santiago de Compostela; España
Fil: Ruiz Parra, Ariel Iván. Universidad Nacional de Colombia; Colombia
Fil: Caminos, Jorge Eduardo. Universidad Nacional de Colombia; Colombia - Materia
-
ENDOGENOUS GHSR ANTAGONIST
GHRELIN/LEAP-2 RATIO
PREGNANCY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/218370
Ver los metadatos del registro completo
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Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancyGarcés, Maria FernandaBuell Acosta, Julieth DanielaÁngel Müller, EdithParada Baños, Arturo JoséAcosta Alvarez, JaidySaavedra López, Harold FelipeFranco Vega, RobertoMaldonado Acosta, Luis MiguelEscobar Cordoba, FranklinVásquez Romero, KeydyLacunza, EzequielCaminos Cepeda, Sofía AlexandraNogueiras, RubénDiéguez, CarlosRuiz Parra, Ariel IvánCaminos, Jorge EduardoENDOGENOUS GHSR ANTAGONISTGHRELIN/LEAP-2 RATIOPREGNANCYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Liver-Expressed Antimicrobial Peptide 2 (LEAP-2) has emerged as an endogenous GHS-R antagonist and blunts the orexigenic action of ghrelin. This study aimed to determine the Ghrelin/LEAP-2 ratio in humans and rats during pregnancy. In humans, we conducted a nested case-control study within an observational prospective cohort. Healthy and mild preeclamptic pregnant women were studied at each trimester of gestation and three months postpartum. In addition, a group of non-pregnant women was studied into the follicular and luteal phases of the menstrual cycle. Furthermore, Ghrelin/LEAP-2 ratio was investigated in non-pregnant rats and at different periods of rat pregnancy. Human and rat serum ghrelin and LEAP-2 levels were determined using the commercially available ELISA kits. The Ghrelin/LEAP-2 ratio peak around the second trimester of gestation in healthy pregnant women (p < 0.05). Additionally, there were no statistically significant differences in Ghrelin/LEAP-2 ratio between healthy and preeclamptic pregnant women at each trimester of gestation (p > 0.05). The Ghrelin/LEAP-2 ratio in pregnant rat reached the peak around mid-gestation with a similar pattern to the human pregnancy. LEAP-2 was visualized by immunohistochemistry in human term placenta and rat placentas on days 12, 16 and 21 of pregnancy. In conclusion, this study provides the first evidence of a Ghrelin/LEAP-2 ratio peak around the half-way point of pregnancy onwards during human and rat pregnancy, and it might be associated with increased rates of weight gain during pregnancy. Thus, this study suggests that LEAP-2 and Ghrelin/LEAP-2 ratio might play an important role in maternal physiology adaptation of weight gain during pregnancy.Fil: Garcés, Maria Fernanda. Universidad Nacional de Colombia; ColombiaFil: Buell Acosta, Julieth Daniela. Universidad Nacional de Colombia; ColombiaFil: Ángel Müller, Edith. Universidad Nacional de Colombia; ColombiaFil: Parada Baños, Arturo José. Universidad Nacional de Colombia; ColombiaFil: Acosta Alvarez, Jaidy. Universidad Nacional de Colombia; ColombiaFil: Saavedra López, Harold Felipe. Universidad Nacional de Colombia; ColombiaFil: Franco Vega, Roberto. Universidad Nacional de Colombia; ColombiaFil: Maldonado Acosta, Luis Miguel. Universidad Nacional de Colombia; ColombiaFil: Escobar Cordoba, Franklin. Universidad Nacional de Colombia; ColombiaFil: Vásquez Romero, Keydy. Universidad de los Andes; ColombiaFil: Lacunza, Ezequiel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Caminos Cepeda, Sofía Alexandra. Universitat Pompeu Fabra; EspañaFil: Nogueiras, Rubén. Universidad de Santiago de Compostela; EspañaFil: Diéguez, Carlos. Universidad de Santiago de Compostela; EspañaFil: Ruiz Parra, Ariel Iván. Universidad Nacional de Colombia; ColombiaFil: Caminos, Jorge Eduardo. Universidad Nacional de Colombia; ColombiaMultidisciplinary Digital Publishing Institute2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/218370Garcés, Maria Fernanda; Buell Acosta, Julieth Daniela; Ángel Müller, Edith; Parada Baños, Arturo José; Acosta Alvarez, Jaidy; et al.; Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 23; 17; 9-2022; 1-121661-65961422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/ijms23179514info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/23/17/9514info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:06Zoai:ri.conicet.gov.ar:11336/218370instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:06.495CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| title |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| spellingShingle |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy Garcés, Maria Fernanda ENDOGENOUS GHSR ANTAGONIST GHRELIN/LEAP-2 RATIO PREGNANCY |
| title_short |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| title_full |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| title_fullStr |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| title_full_unstemmed |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| title_sort |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| dc.creator.none.fl_str_mv |
Garcés, Maria Fernanda Buell Acosta, Julieth Daniela Ángel Müller, Edith Parada Baños, Arturo José Acosta Alvarez, Jaidy Saavedra López, Harold Felipe Franco Vega, Roberto Maldonado Acosta, Luis Miguel Escobar Cordoba, Franklin Vásquez Romero, Keydy Lacunza, Ezequiel Caminos Cepeda, Sofía Alexandra Nogueiras, Rubén Diéguez, Carlos Ruiz Parra, Ariel Iván Caminos, Jorge Eduardo |
| author |
Garcés, Maria Fernanda |
| author_facet |
Garcés, Maria Fernanda Buell Acosta, Julieth Daniela Ángel Müller, Edith Parada Baños, Arturo José Acosta Alvarez, Jaidy Saavedra López, Harold Felipe Franco Vega, Roberto Maldonado Acosta, Luis Miguel Escobar Cordoba, Franklin Vásquez Romero, Keydy Lacunza, Ezequiel Caminos Cepeda, Sofía Alexandra Nogueiras, Rubén Diéguez, Carlos Ruiz Parra, Ariel Iván Caminos, Jorge Eduardo |
| author_role |
author |
| author2 |
Buell Acosta, Julieth Daniela Ángel Müller, Edith Parada Baños, Arturo José Acosta Alvarez, Jaidy Saavedra López, Harold Felipe Franco Vega, Roberto Maldonado Acosta, Luis Miguel Escobar Cordoba, Franklin Vásquez Romero, Keydy Lacunza, Ezequiel Caminos Cepeda, Sofía Alexandra Nogueiras, Rubén Diéguez, Carlos Ruiz Parra, Ariel Iván Caminos, Jorge Eduardo |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
ENDOGENOUS GHSR ANTAGONIST GHRELIN/LEAP-2 RATIO PREGNANCY |
| topic |
ENDOGENOUS GHSR ANTAGONIST GHRELIN/LEAP-2 RATIO PREGNANCY |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
The Liver-Expressed Antimicrobial Peptide 2 (LEAP-2) has emerged as an endogenous GHS-R antagonist and blunts the orexigenic action of ghrelin. This study aimed to determine the Ghrelin/LEAP-2 ratio in humans and rats during pregnancy. In humans, we conducted a nested case-control study within an observational prospective cohort. Healthy and mild preeclamptic pregnant women were studied at each trimester of gestation and three months postpartum. In addition, a group of non-pregnant women was studied into the follicular and luteal phases of the menstrual cycle. Furthermore, Ghrelin/LEAP-2 ratio was investigated in non-pregnant rats and at different periods of rat pregnancy. Human and rat serum ghrelin and LEAP-2 levels were determined using the commercially available ELISA kits. The Ghrelin/LEAP-2 ratio peak around the second trimester of gestation in healthy pregnant women (p < 0.05). Additionally, there were no statistically significant differences in Ghrelin/LEAP-2 ratio between healthy and preeclamptic pregnant women at each trimester of gestation (p > 0.05). The Ghrelin/LEAP-2 ratio in pregnant rat reached the peak around mid-gestation with a similar pattern to the human pregnancy. LEAP-2 was visualized by immunohistochemistry in human term placenta and rat placentas on days 12, 16 and 21 of pregnancy. In conclusion, this study provides the first evidence of a Ghrelin/LEAP-2 ratio peak around the half-way point of pregnancy onwards during human and rat pregnancy, and it might be associated with increased rates of weight gain during pregnancy. Thus, this study suggests that LEAP-2 and Ghrelin/LEAP-2 ratio might play an important role in maternal physiology adaptation of weight gain during pregnancy. Fil: Garcés, Maria Fernanda. Universidad Nacional de Colombia; Colombia Fil: Buell Acosta, Julieth Daniela. Universidad Nacional de Colombia; Colombia Fil: Ángel Müller, Edith. Universidad Nacional de Colombia; Colombia Fil: Parada Baños, Arturo José. Universidad Nacional de Colombia; Colombia Fil: Acosta Alvarez, Jaidy. Universidad Nacional de Colombia; Colombia Fil: Saavedra López, Harold Felipe. Universidad Nacional de Colombia; Colombia Fil: Franco Vega, Roberto. Universidad Nacional de Colombia; Colombia Fil: Maldonado Acosta, Luis Miguel. Universidad Nacional de Colombia; Colombia Fil: Escobar Cordoba, Franklin. Universidad Nacional de Colombia; Colombia Fil: Vásquez Romero, Keydy. Universidad de los Andes; Colombia Fil: Lacunza, Ezequiel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Caminos Cepeda, Sofía Alexandra. Universitat Pompeu Fabra; España Fil: Nogueiras, Rubén. Universidad de Santiago de Compostela; España Fil: Diéguez, Carlos. Universidad de Santiago de Compostela; España Fil: Ruiz Parra, Ariel Iván. Universidad Nacional de Colombia; Colombia Fil: Caminos, Jorge Eduardo. Universidad Nacional de Colombia; Colombia |
| description |
The Liver-Expressed Antimicrobial Peptide 2 (LEAP-2) has emerged as an endogenous GHS-R antagonist and blunts the orexigenic action of ghrelin. This study aimed to determine the Ghrelin/LEAP-2 ratio in humans and rats during pregnancy. In humans, we conducted a nested case-control study within an observational prospective cohort. Healthy and mild preeclamptic pregnant women were studied at each trimester of gestation and three months postpartum. In addition, a group of non-pregnant women was studied into the follicular and luteal phases of the menstrual cycle. Furthermore, Ghrelin/LEAP-2 ratio was investigated in non-pregnant rats and at different periods of rat pregnancy. Human and rat serum ghrelin and LEAP-2 levels were determined using the commercially available ELISA kits. The Ghrelin/LEAP-2 ratio peak around the second trimester of gestation in healthy pregnant women (p < 0.05). Additionally, there were no statistically significant differences in Ghrelin/LEAP-2 ratio between healthy and preeclamptic pregnant women at each trimester of gestation (p > 0.05). The Ghrelin/LEAP-2 ratio in pregnant rat reached the peak around mid-gestation with a similar pattern to the human pregnancy. LEAP-2 was visualized by immunohistochemistry in human term placenta and rat placentas on days 12, 16 and 21 of pregnancy. In conclusion, this study provides the first evidence of a Ghrelin/LEAP-2 ratio peak around the half-way point of pregnancy onwards during human and rat pregnancy, and it might be associated with increased rates of weight gain during pregnancy. Thus, this study suggests that LEAP-2 and Ghrelin/LEAP-2 ratio might play an important role in maternal physiology adaptation of weight gain during pregnancy. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-09 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/218370 Garcés, Maria Fernanda; Buell Acosta, Julieth Daniela; Ángel Müller, Edith; Parada Baños, Arturo José; Acosta Alvarez, Jaidy; et al.; Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 23; 17; 9-2022; 1-12 1661-6596 1422-0067 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/218370 |
| identifier_str_mv |
Garcés, Maria Fernanda; Buell Acosta, Julieth Daniela; Ángel Müller, Edith; Parada Baños, Arturo José; Acosta Alvarez, Jaidy; et al.; Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 23; 17; 9-2022; 1-12 1661-6596 1422-0067 CONICET Digital CONICET |
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eng |
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Multidisciplinary Digital Publishing Institute |
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Multidisciplinary Digital Publishing Institute |
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