Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage

Autores
Salido, Ezequiel Martin; Dorfman, Damián; Bordone, Melina Paula; Chianelli, Monica Silvia; González Fleitas, María Florencia; Rosenstein, Ruth Estela
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Retinal ischemia could provoke blindness. At present, there is no effective treatment against retinal ischemic damage. Strong evidence supports that glutamate is implicated in retinal ischemic damage. We investigated whether a brief period of global or ocular hypothermia applied 24 h before ischemia (i.e. hypothermic preconditioning, HPC) protects the retina from ischemia/reperfusion damage, and the involvement of glutamate in the retinal protection induced by HPC. For this purpose, ischemia was induced by increasing intraocular pressure to 120 mm Hg for 40 min. One day before ischemia, animals were submitted to global or ocular hypothermia (33uC and 32uC for 20 min, respectively) and fourteen days after ischemia, animals were subjected to electroretinography and histological analysis. Global or ocular HPC afforded significant functional (electroretinographic) protection in eyes exposed to ischemia/reperfusion injury. A marked alteration of the retinal structure and a decrease in retinal ganglion cell number were observed in ischemic retinas, whereas global or ocular HPC significantly preserved retinal structure and ganglion cell count. Three days after ischemia, a significant decrease in retinal glutamate uptake and glutamine synthetase activity was observed, whereas ocular HPC prevented the effect of ischemia on these parameters. The intravitreal injection of supraphysiological levels of glutamate induced alterations in retinal function and histology which were significantly prevented by ocular HPC. These results support that global or ocular HPC significantly protected retinal function and histology from ischemia/reperfusion injury, probably through a glutamatedependent mechanism.
Fil: Salido E.
Fil: Dorfman D.
Fil: Bordone, Melina.
Fil: Chianelli Mónica S.
Fil: González Fleitas Mf.
Fil: Rosenstein Re.
Materia
ischemia retinal
preconditioning
hypothermia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/521
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/521
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic DamageSalido, Ezequiel MartinDorfman, DamiánBordone, Melina PaulaChianelli, Monica SilviaGonzález Fleitas, María FlorenciaRosenstein, Ruth Estelaischemia retinalpreconditioninghypothermiahttps://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1Retinal ischemia could provoke blindness. At present, there is no effective treatment against retinal ischemic damage. Strong evidence supports that glutamate is implicated in retinal ischemic damage. We investigated whether a brief period of global or ocular hypothermia applied 24 h before ischemia (i.e. hypothermic preconditioning, HPC) protects the retina from ischemia/reperfusion damage, and the involvement of glutamate in the retinal protection induced by HPC. For this purpose, ischemia was induced by increasing intraocular pressure to 120 mm Hg for 40 min. One day before ischemia, animals were submitted to global or ocular hypothermia (33uC and 32uC for 20 min, respectively) and fourteen days after ischemia, animals were subjected to electroretinography and histological analysis. Global or ocular HPC afforded significant functional (electroretinographic) protection in eyes exposed to ischemia/reperfusion injury. A marked alteration of the retinal structure and a decrease in retinal ganglion cell number were observed in ischemic retinas, whereas global or ocular HPC significantly preserved retinal structure and ganglion cell count. Three days after ischemia, a significant decrease in retinal glutamate uptake and glutamine synthetase activity was observed, whereas ocular HPC prevented the effect of ischemia on these parameters. The intravitreal injection of supraphysiological levels of glutamate induced alterations in retinal function and histology which were significantly prevented by ocular HPC. These results support that global or ocular HPC significantly protected retinal function and histology from ischemia/reperfusion injury, probably through a glutamatedependent mechanism.Fil: Salido E.Fil: Dorfman D.Fil: Bordone, Melina.Fil: Chianelli Mónica S.Fil: González Fleitas Mf.Fil: Rosenstein Re.Public Library Science2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/521Salido E; Dorfman D; Bordone, Melina; Chianelli Mónica S; González Fleitas Mf; Rosenstein Re; Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage; Public Library Science; Plos One; 8; 4; 2013-4; 61656-61668;1932-6203enginfo:eu-repo/semantics/altIdentifier/url/www.plosone.orginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:12Zoai:ri.conicet.gov.ar:11336/521instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:12.765CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage
title Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage
spellingShingle Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage
Salido, Ezequiel Martin
ischemia retinal
preconditioning
hypothermia
title_short Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage
title_full Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage
title_fullStr Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage
title_full_unstemmed Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage
title_sort Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage
dc.creator.none.fl_str_mv Salido, Ezequiel Martin
Dorfman, Damián
Bordone, Melina Paula
Chianelli, Monica Silvia
González Fleitas, María Florencia
Rosenstein, Ruth Estela
author Salido, Ezequiel Martin
author_facet Salido, Ezequiel Martin
Dorfman, Damián
Bordone, Melina Paula
Chianelli, Monica Silvia
González Fleitas, María Florencia
Rosenstein, Ruth Estela
author_role author
author2 Dorfman, Damián
Bordone, Melina Paula
Chianelli, Monica Silvia
González Fleitas, María Florencia
Rosenstein, Ruth Estela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ischemia retinal
preconditioning
hypothermia
topic ischemia retinal
preconditioning
hypothermia
purl_subject.fl_str_mv https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
dc.description.none.fl_txt_mv Retinal ischemia could provoke blindness. At present, there is no effective treatment against retinal ischemic damage. Strong evidence supports that glutamate is implicated in retinal ischemic damage. We investigated whether a brief period of global or ocular hypothermia applied 24 h before ischemia (i.e. hypothermic preconditioning, HPC) protects the retina from ischemia/reperfusion damage, and the involvement of glutamate in the retinal protection induced by HPC. For this purpose, ischemia was induced by increasing intraocular pressure to 120 mm Hg for 40 min. One day before ischemia, animals were submitted to global or ocular hypothermia (33uC and 32uC for 20 min, respectively) and fourteen days after ischemia, animals were subjected to electroretinography and histological analysis. Global or ocular HPC afforded significant functional (electroretinographic) protection in eyes exposed to ischemia/reperfusion injury. A marked alteration of the retinal structure and a decrease in retinal ganglion cell number were observed in ischemic retinas, whereas global or ocular HPC significantly preserved retinal structure and ganglion cell count. Three days after ischemia, a significant decrease in retinal glutamate uptake and glutamine synthetase activity was observed, whereas ocular HPC prevented the effect of ischemia on these parameters. The intravitreal injection of supraphysiological levels of glutamate induced alterations in retinal function and histology which were significantly prevented by ocular HPC. These results support that global or ocular HPC significantly protected retinal function and histology from ischemia/reperfusion injury, probably through a glutamatedependent mechanism.
Fil: Salido E.
Fil: Dorfman D.
Fil: Bordone, Melina.
Fil: Chianelli Mónica S.
Fil: González Fleitas Mf.
Fil: Rosenstein Re.
description Retinal ischemia could provoke blindness. At present, there is no effective treatment against retinal ischemic damage. Strong evidence supports that glutamate is implicated in retinal ischemic damage. We investigated whether a brief period of global or ocular hypothermia applied 24 h before ischemia (i.e. hypothermic preconditioning, HPC) protects the retina from ischemia/reperfusion damage, and the involvement of glutamate in the retinal protection induced by HPC. For this purpose, ischemia was induced by increasing intraocular pressure to 120 mm Hg for 40 min. One day before ischemia, animals were submitted to global or ocular hypothermia (33uC and 32uC for 20 min, respectively) and fourteen days after ischemia, animals were subjected to electroretinography and histological analysis. Global or ocular HPC afforded significant functional (electroretinographic) protection in eyes exposed to ischemia/reperfusion injury. A marked alteration of the retinal structure and a decrease in retinal ganglion cell number were observed in ischemic retinas, whereas global or ocular HPC significantly preserved retinal structure and ganglion cell count. Three days after ischemia, a significant decrease in retinal glutamate uptake and glutamine synthetase activity was observed, whereas ocular HPC prevented the effect of ischemia on these parameters. The intravitreal injection of supraphysiological levels of glutamate induced alterations in retinal function and histology which were significantly prevented by ocular HPC. These results support that global or ocular HPC significantly protected retinal function and histology from ischemia/reperfusion injury, probably through a glutamatedependent mechanism.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/521
Salido E; Dorfman D; Bordone, Melina; Chianelli Mónica S; González Fleitas Mf; Rosenstein Re; Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage; Public Library Science; Plos One; 8; 4; 2013-4; 61656-61668;
1932-6203
url http://hdl.handle.net/11336/521
identifier_str_mv Salido E; Dorfman D; Bordone, Melina; Chianelli Mónica S; González Fleitas Mf; Rosenstein Re; Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage; Public Library Science; Plos One; 8; 4; 2013-4; 61656-61668;
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/www.plosone.org
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269019584856064
score 13.13397

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