Screening and Selection of Wild Strains for L-arabinose isomerase production
- Autores
- Manzo, Ricardo Martín; Simonetta, Arturo Carlos; Rubiolo, Amelia Catalina; Mammarella, Enrique José
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The majority of L-arabinose isomerases have been isolated by recombinant techniques, but this methodology implies a reduced technological application. For this reason, 29 bacterial strains, some of them previously characterized as L-arabinose isomerase producers, were assayed as L-arabinose fermenting strains by employing conveniently designed culture media with 0.5% (w/v) L-arabinose as main carbon source. From all evaluated bacterial strains, Enterococcus faecium DBFIQ ID: E36, Enterococcus faecium DBFIQ ID: ETW4 and Pediococcus acidilactici ATCC ID: 8042 were, in this order, the best L-arabinose fermenting strains. Afterwards, to assay L-arabinose metabolization and L-arabinose isomerase activity, cell-free extract and saline precipitated cell-free extract of the three bacterial cultures were obtained and the production of ketoses was determined by the cysteine carbazole sulfuric acid method. Results showed that the greater the L-arabinose metabolization ability, the higher the enzymatic activity achieved, so Enterococcus faecium DBFIQ ID: E36 was selected to continue with production, purification and characterization studies. This work thus describes a simple microbiological method for the selection of L-arabinose fermenting bacteria for the potential production of the enzyme L-arabinose isomerase.
Fil: Manzo, Ricardo Martín. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Ingeniería en Alimento. Area de Biotecnología; Argentina;
Fil: Simonetta, Arturo Carlos. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Ingeniería en Alimento. Area de Biotecnología; Argentina;
Fil: Rubiolo, Amelia Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina;
Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina; - Materia
-
L-arabinose isomerase
D-galactose
T-tagatose
Whey proteins
Cheese whey
Microbiological method - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/1836
Ver los metadatos del registro completo
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Screening and Selection of Wild Strains for L-arabinose isomerase productionManzo, Ricardo MartínSimonetta, Arturo CarlosRubiolo, Amelia CatalinaMammarella, Enrique JoséL-arabinose isomeraseD-galactoseT-tagatoseWhey proteinsCheese wheyMicrobiological methodhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2The majority of L-arabinose isomerases have been isolated by recombinant techniques, but this methodology implies a reduced technological application. For this reason, 29 bacterial strains, some of them previously characterized as L-arabinose isomerase producers, were assayed as L-arabinose fermenting strains by employing conveniently designed culture media with 0.5% (w/v) L-arabinose as main carbon source. From all evaluated bacterial strains, Enterococcus faecium DBFIQ ID: E36, Enterococcus faecium DBFIQ ID: ETW4 and Pediococcus acidilactici ATCC ID: 8042 were, in this order, the best L-arabinose fermenting strains. Afterwards, to assay L-arabinose metabolization and L-arabinose isomerase activity, cell-free extract and saline precipitated cell-free extract of the three bacterial cultures were obtained and the production of ketoses was determined by the cysteine carbazole sulfuric acid method. Results showed that the greater the L-arabinose metabolization ability, the higher the enzymatic activity achieved, so Enterococcus faecium DBFIQ ID: E36 was selected to continue with production, purification and characterization studies. This work thus describes a simple microbiological method for the selection of L-arabinose fermenting bacteria for the potential production of the enzyme L-arabinose isomerase.Fil: Manzo, Ricardo Martín. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Ingeniería en Alimento. Area de Biotecnología; Argentina;Fil: Simonetta, Arturo Carlos. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Ingeniería en Alimento. Area de Biotecnología; Argentina;Fil: Rubiolo, Amelia Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina;Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina;Brazilian Society of Chemical Engineering2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1836Manzo, Ricardo Martín; Simonetta, Arturo Carlos; Rubiolo, Amelia Catalina; Mammarella, Enrique José; Screening and Selection of Wild Strains for L-arabinose isomerase production; Brazilian Society of Chemical Engineering; Brazilian Journal of Chemical Engineering; 30; 4; 10-2013; 711-7200104-6632enginfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?pid=S0104-66322013000400003&script=sci_arttextinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:30:12Zoai:ri.conicet.gov.ar:11336/1836instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:30:12.361CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Screening and Selection of Wild Strains for L-arabinose isomerase production |
| title |
Screening and Selection of Wild Strains for L-arabinose isomerase production |
| spellingShingle |
Screening and Selection of Wild Strains for L-arabinose isomerase production Manzo, Ricardo Martín L-arabinose isomerase D-galactose T-tagatose Whey proteins Cheese whey Microbiological method |
| title_short |
Screening and Selection of Wild Strains for L-arabinose isomerase production |
| title_full |
Screening and Selection of Wild Strains for L-arabinose isomerase production |
| title_fullStr |
Screening and Selection of Wild Strains for L-arabinose isomerase production |
| title_full_unstemmed |
Screening and Selection of Wild Strains for L-arabinose isomerase production |
| title_sort |
Screening and Selection of Wild Strains for L-arabinose isomerase production |
| dc.creator.none.fl_str_mv |
Manzo, Ricardo Martín Simonetta, Arturo Carlos Rubiolo, Amelia Catalina Mammarella, Enrique José |
| author |
Manzo, Ricardo Martín |
| author_facet |
Manzo, Ricardo Martín Simonetta, Arturo Carlos Rubiolo, Amelia Catalina Mammarella, Enrique José |
| author_role |
author |
| author2 |
Simonetta, Arturo Carlos Rubiolo, Amelia Catalina Mammarella, Enrique José |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
L-arabinose isomerase D-galactose T-tagatose Whey proteins Cheese whey Microbiological method |
| topic |
L-arabinose isomerase D-galactose T-tagatose Whey proteins Cheese whey Microbiological method |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.4 https://purl.org/becyt/ford/2 |
| dc.description.none.fl_txt_mv |
The majority of L-arabinose isomerases have been isolated by recombinant techniques, but this methodology implies a reduced technological application. For this reason, 29 bacterial strains, some of them previously characterized as L-arabinose isomerase producers, were assayed as L-arabinose fermenting strains by employing conveniently designed culture media with 0.5% (w/v) L-arabinose as main carbon source. From all evaluated bacterial strains, Enterococcus faecium DBFIQ ID: E36, Enterococcus faecium DBFIQ ID: ETW4 and Pediococcus acidilactici ATCC ID: 8042 were, in this order, the best L-arabinose fermenting strains. Afterwards, to assay L-arabinose metabolization and L-arabinose isomerase activity, cell-free extract and saline precipitated cell-free extract of the three bacterial cultures were obtained and the production of ketoses was determined by the cysteine carbazole sulfuric acid method. Results showed that the greater the L-arabinose metabolization ability, the higher the enzymatic activity achieved, so Enterococcus faecium DBFIQ ID: E36 was selected to continue with production, purification and characterization studies. This work thus describes a simple microbiological method for the selection of L-arabinose fermenting bacteria for the potential production of the enzyme L-arabinose isomerase. Fil: Manzo, Ricardo Martín. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Ingeniería en Alimento. Area de Biotecnología; Argentina; Fil: Simonetta, Arturo Carlos. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Ingeniería en Alimento. Area de Biotecnología; Argentina; Fil: Rubiolo, Amelia Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina; Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina; |
| description |
The majority of L-arabinose isomerases have been isolated by recombinant techniques, but this methodology implies a reduced technological application. For this reason, 29 bacterial strains, some of them previously characterized as L-arabinose isomerase producers, were assayed as L-arabinose fermenting strains by employing conveniently designed culture media with 0.5% (w/v) L-arabinose as main carbon source. From all evaluated bacterial strains, Enterococcus faecium DBFIQ ID: E36, Enterococcus faecium DBFIQ ID: ETW4 and Pediococcus acidilactici ATCC ID: 8042 were, in this order, the best L-arabinose fermenting strains. Afterwards, to assay L-arabinose metabolization and L-arabinose isomerase activity, cell-free extract and saline precipitated cell-free extract of the three bacterial cultures were obtained and the production of ketoses was determined by the cysteine carbazole sulfuric acid method. Results showed that the greater the L-arabinose metabolization ability, the higher the enzymatic activity achieved, so Enterococcus faecium DBFIQ ID: E36 was selected to continue with production, purification and characterization studies. This work thus describes a simple microbiological method for the selection of L-arabinose fermenting bacteria for the potential production of the enzyme L-arabinose isomerase. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013-10 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/1836 Manzo, Ricardo Martín; Simonetta, Arturo Carlos; Rubiolo, Amelia Catalina; Mammarella, Enrique José; Screening and Selection of Wild Strains for L-arabinose isomerase production; Brazilian Society of Chemical Engineering; Brazilian Journal of Chemical Engineering; 30; 4; 10-2013; 711-720 0104-6632 |
| url |
http://hdl.handle.net/11336/1836 |
| identifier_str_mv |
Manzo, Ricardo Martín; Simonetta, Arturo Carlos; Rubiolo, Amelia Catalina; Mammarella, Enrique José; Screening and Selection of Wild Strains for L-arabinose isomerase production; Brazilian Society of Chemical Engineering; Brazilian Journal of Chemical Engineering; 30; 4; 10-2013; 711-720 0104-6632 |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?pid=S0104-66322013000400003&script=sci_arttext |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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application/pdf application/pdf |
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Brazilian Society of Chemical Engineering |
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Brazilian Society of Chemical Engineering |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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