Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis
- Autores
- de Souza, Marylane; Manzo, Ricardo Martín; Garcia, Jose Luis; Mammarella, Enrique José; Gonçalves, Luciana; Pessela, Benevides
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- l-Arabinose isomerase (EC 5.3.1.4) (l-AI) from Enterococcus faecium DBFIQ E36 was overproduced in Escherichia coli by designing a codon-optimized synthetic araA gene. Using this optimized gene, two N- and C-terminal His-tagged-l-AI proteins were produced. The cloning of the two chimeric genes into regulated expression vectors resulted in the production of high amounts of recombinant N-His-l-AI and C-His-l-AI in soluble and active forms. Both His-tagged enzymes were purified in a single step through metal-affinity chromatography and showed different kinetic and structural characteristics. Analytical ultracentrifugation revealed that C-His-l-AI was preferentially hexameric in solution, whereas N-His-l-AI was mainly monomeric. The specific activity of the N-His-l-AI at acidic pH was higher than that of C-His-l-AI and showed a maximum bioconversion yield of 26% at 50 °C for d-tagatose biosynthesis, with Km and Vmax parameters of 252 mM and 0.092 U mg−1, respectively. However, C-His-l-AI was more active and stable at alkaline pH than N-His-l-AI. N-His-l-AI follows a Michaelis-Menten kinetic, whereas C-His-l-AI fitted to a sigmoidal saturation curve.
Fil: de Souza, Marylane. Universidade Federal de Ceará;
Fil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Garcia, Jose Luis. Higher Council for Scientific Research; España
Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Gonçalves, Luciana. Universidade Federal de Ceará;
Fil: Pessela, Benevides. Higher Council for Scientific Research; España - Materia
-
L-ARABINOSE ISOMERASE
RECOMBINANT DNA
AFFINITY PURIFICATION
D-TAGATOSE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/33578
Ver los metadatos del registro completo
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Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesisde Souza, MarylaneManzo, Ricardo MartínGarcia, Jose LuisMammarella, Enrique JoséGonçalves, LucianaPessela, BenevidesL-ARABINOSE ISOMERASERECOMBINANT DNAAFFINITY PURIFICATIOND-TAGATOSEhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2l-Arabinose isomerase (EC 5.3.1.4) (l-AI) from Enterococcus faecium DBFIQ E36 was overproduced in Escherichia coli by designing a codon-optimized synthetic araA gene. Using this optimized gene, two N- and C-terminal His-tagged-l-AI proteins were produced. The cloning of the two chimeric genes into regulated expression vectors resulted in the production of high amounts of recombinant N-His-l-AI and C-His-l-AI in soluble and active forms. Both His-tagged enzymes were purified in a single step through metal-affinity chromatography and showed different kinetic and structural characteristics. Analytical ultracentrifugation revealed that C-His-l-AI was preferentially hexameric in solution, whereas N-His-l-AI was mainly monomeric. The specific activity of the N-His-l-AI at acidic pH was higher than that of C-His-l-AI and showed a maximum bioconversion yield of 26% at 50 °C for d-tagatose biosynthesis, with Km and Vmax parameters of 252 mM and 0.092 U mg−1, respectively. However, C-His-l-AI was more active and stable at alkaline pH than N-His-l-AI. N-His-l-AI follows a Michaelis-Menten kinetic, whereas C-His-l-AI fitted to a sigmoidal saturation curve.Fil: de Souza, Marylane. Universidade Federal de Ceará;Fil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Garcia, Jose Luis. Higher Council for Scientific Research; EspañaFil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Gonçalves, Luciana. Universidade Federal de Ceará;Fil: Pessela, Benevides. Higher Council for Scientific Research; EspañaMolecular Diversity Preservation International2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33578Garcia, Jose Luis; Mammarella, Enrique José; Gonçalves, Luciana; Manzo, Ricardo Martín; de Souza, Marylane; Pessela, Benevides; et al.; Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis; Molecular Diversity Preservation International; Molecules; 22; 12; 12-2017; 21641420-3049CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1420-3049/22/12/2164info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules22122164info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:22:58Zoai:ri.conicet.gov.ar:11336/33578instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:22:58.451CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis |
| title |
Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis |
| spellingShingle |
Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis de Souza, Marylane L-ARABINOSE ISOMERASE RECOMBINANT DNA AFFINITY PURIFICATION D-TAGATOSE |
| title_short |
Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis |
| title_full |
Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis |
| title_fullStr |
Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis |
| title_full_unstemmed |
Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis |
| title_sort |
Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis |
| dc.creator.none.fl_str_mv |
de Souza, Marylane Manzo, Ricardo Martín Garcia, Jose Luis Mammarella, Enrique José Gonçalves, Luciana Pessela, Benevides |
| author |
de Souza, Marylane |
| author_facet |
de Souza, Marylane Manzo, Ricardo Martín Garcia, Jose Luis Mammarella, Enrique José Gonçalves, Luciana Pessela, Benevides |
| author_role |
author |
| author2 |
Manzo, Ricardo Martín Garcia, Jose Luis Mammarella, Enrique José Gonçalves, Luciana Pessela, Benevides |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
L-ARABINOSE ISOMERASE RECOMBINANT DNA AFFINITY PURIFICATION D-TAGATOSE |
| topic |
L-ARABINOSE ISOMERASE RECOMBINANT DNA AFFINITY PURIFICATION D-TAGATOSE |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
| dc.description.none.fl_txt_mv |
l-Arabinose isomerase (EC 5.3.1.4) (l-AI) from Enterococcus faecium DBFIQ E36 was overproduced in Escherichia coli by designing a codon-optimized synthetic araA gene. Using this optimized gene, two N- and C-terminal His-tagged-l-AI proteins were produced. The cloning of the two chimeric genes into regulated expression vectors resulted in the production of high amounts of recombinant N-His-l-AI and C-His-l-AI in soluble and active forms. Both His-tagged enzymes were purified in a single step through metal-affinity chromatography and showed different kinetic and structural characteristics. Analytical ultracentrifugation revealed that C-His-l-AI was preferentially hexameric in solution, whereas N-His-l-AI was mainly monomeric. The specific activity of the N-His-l-AI at acidic pH was higher than that of C-His-l-AI and showed a maximum bioconversion yield of 26% at 50 °C for d-tagatose biosynthesis, with Km and Vmax parameters of 252 mM and 0.092 U mg−1, respectively. However, C-His-l-AI was more active and stable at alkaline pH than N-His-l-AI. N-His-l-AI follows a Michaelis-Menten kinetic, whereas C-His-l-AI fitted to a sigmoidal saturation curve. Fil: de Souza, Marylane. Universidade Federal de Ceará; Fil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: Garcia, Jose Luis. Higher Council for Scientific Research; España Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: Gonçalves, Luciana. Universidade Federal de Ceará; Fil: Pessela, Benevides. Higher Council for Scientific Research; España |
| description |
l-Arabinose isomerase (EC 5.3.1.4) (l-AI) from Enterococcus faecium DBFIQ E36 was overproduced in Escherichia coli by designing a codon-optimized synthetic araA gene. Using this optimized gene, two N- and C-terminal His-tagged-l-AI proteins were produced. The cloning of the two chimeric genes into regulated expression vectors resulted in the production of high amounts of recombinant N-His-l-AI and C-His-l-AI in soluble and active forms. Both His-tagged enzymes were purified in a single step through metal-affinity chromatography and showed different kinetic and structural characteristics. Analytical ultracentrifugation revealed that C-His-l-AI was preferentially hexameric in solution, whereas N-His-l-AI was mainly monomeric. The specific activity of the N-His-l-AI at acidic pH was higher than that of C-His-l-AI and showed a maximum bioconversion yield of 26% at 50 °C for d-tagatose biosynthesis, with Km and Vmax parameters of 252 mM and 0.092 U mg−1, respectively. However, C-His-l-AI was more active and stable at alkaline pH than N-His-l-AI. N-His-l-AI follows a Michaelis-Menten kinetic, whereas C-His-l-AI fitted to a sigmoidal saturation curve. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-12 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/33578 Garcia, Jose Luis; Mammarella, Enrique José; Gonçalves, Luciana; Manzo, Ricardo Martín; de Souza, Marylane; Pessela, Benevides; et al.; Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis; Molecular Diversity Preservation International; Molecules; 22; 12; 12-2017; 2164 1420-3049 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/33578 |
| identifier_str_mv |
Garcia, Jose Luis; Mammarella, Enrique José; Gonçalves, Luciana; Manzo, Ricardo Martín; de Souza, Marylane; Pessela, Benevides; et al.; Engineering the L-Arabinose Isomerase from Enterococcus Faecium for D-Tagatose Synthesis; Molecular Diversity Preservation International; Molecules; 22; 12; 12-2017; 2164 1420-3049 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1420-3049/22/12/2164 info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules22122164 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf |
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Molecular Diversity Preservation International |
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Molecular Diversity Preservation International |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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