Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro

Autores
Lordello, Virgínia Barreto; Meneguin, Andréia Bagliotti; de Annunzio, Sarah Raquel; Taranto, Maria Pia; Chorilli, Marlus; Fontana, Carla Raquel; Cavallini, Daniela Cardoso Umbelino
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1 ), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.
Fil: Lordello, Virgínia Barreto. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Meneguin, Andréia Bagliotti. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: de Annunzio, Sarah Raquel. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Taranto, Maria Pia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Chorilli, Marlus. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Fontana, Carla Raquel. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Cavallini, Daniela Cardoso Umbelino. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Materia
ANTI-CANDIDA ACTIVITY
BIOFILM
CANDIDA ALBICANS
ENTEROCOC-CUS FAECIUM
ORODISPERSIBLE FILMS
PROBIOTICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/205855
Acceder
id CONICETDig_b2c3c2f8104bdfd93e220345d9571e9f
oai_identifier_str oai:ri.conicet.gov.ar:11336/205855
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitroLordello, Virgínia BarretoMeneguin, Andréia Bagliottide Annunzio, Sarah RaquelTaranto, Maria PiaChorilli, MarlusFontana, Carla RaquelCavallini, Daniela Cardoso UmbelinoANTI-CANDIDA ACTIVITYBIOFILMCANDIDA ALBICANSENTEROCOC-CUS FAECIUMORODISPERSIBLE FILMSPROBIOTICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1 ), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.Fil: Lordello, Virgínia Barreto. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Meneguin, Andréia Bagliotti. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: de Annunzio, Sarah Raquel. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Taranto, Maria Pia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Chorilli, Marlus. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Fontana, Carla Raquel. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Cavallini, Daniela Cardoso Umbelino. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilMultidisciplinary Digital Publishing Institute2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/205855Lordello, Virgínia Barreto; Meneguin, Andréia Bagliotti; de Annunzio, Sarah Raquel; Taranto, Maria Pia; Chorilli, Marlus; et al.; Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro; Multidisciplinary Digital Publishing Institute; Pharmaceutics; 13; 7; 7-2021; 1-161999-49231999-4923CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/13/7/998info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics13070998info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:52:02Zoai:ri.conicet.gov.ar:11336/205855instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:52:02.936CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro
title Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro
spellingShingle Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro
Lordello, Virgínia Barreto
ANTI-CANDIDA ACTIVITY
BIOFILM
CANDIDA ALBICANS
ENTEROCOC-CUS FAECIUM
ORODISPERSIBLE FILMS
PROBIOTICS
title_short Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro
title_full Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro
title_fullStr Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro
title_full_unstemmed Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro
title_sort Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro
dc.creator.none.fl_str_mv Lordello, Virgínia Barreto
Meneguin, Andréia Bagliotti
de Annunzio, Sarah Raquel
Taranto, Maria Pia
Chorilli, Marlus
Fontana, Carla Raquel
Cavallini, Daniela Cardoso Umbelino
author Lordello, Virgínia Barreto
author_facet Lordello, Virgínia Barreto
Meneguin, Andréia Bagliotti
de Annunzio, Sarah Raquel
Taranto, Maria Pia
Chorilli, Marlus
Fontana, Carla Raquel
Cavallini, Daniela Cardoso Umbelino
author_role author
author2 Meneguin, Andréia Bagliotti
de Annunzio, Sarah Raquel
Taranto, Maria Pia
Chorilli, Marlus
Fontana, Carla Raquel
Cavallini, Daniela Cardoso Umbelino
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTI-CANDIDA ACTIVITY
BIOFILM
CANDIDA ALBICANS
ENTEROCOC-CUS FAECIUM
ORODISPERSIBLE FILMS
PROBIOTICS
topic ANTI-CANDIDA ACTIVITY
BIOFILM
CANDIDA ALBICANS
ENTEROCOC-CUS FAECIUM
ORODISPERSIBLE FILMS
PROBIOTICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1 ), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.
Fil: Lordello, Virgínia Barreto. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Meneguin, Andréia Bagliotti. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: de Annunzio, Sarah Raquel. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Taranto, Maria Pia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Chorilli, Marlus. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Fontana, Carla Raquel. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Cavallini, Daniela Cardoso Umbelino. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
description Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1 ), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.
publishDate 2021
dc.date.none.fl_str_mv 2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/205855
Lordello, Virgínia Barreto; Meneguin, Andréia Bagliotti; de Annunzio, Sarah Raquel; Taranto, Maria Pia; Chorilli, Marlus; et al.; Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro; Multidisciplinary Digital Publishing Institute; Pharmaceutics; 13; 7; 7-2021; 1-16
1999-4923
1999-4923
CONICET Digital
CONICET
url http://hdl.handle.net/11336/205855
identifier_str_mv Lordello, Virgínia Barreto; Meneguin, Andréia Bagliotti; de Annunzio, Sarah Raquel; Taranto, Maria Pia; Chorilli, Marlus; et al.; Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro; Multidisciplinary Digital Publishing Institute; Pharmaceutics; 13; 7; 7-2021; 1-16
1999-4923
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/13/7/998
info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics13070998
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782209949696000
score 12.982451

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