Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast
- Autores
- Thoke, Henrik S.; Bagatolli, Luis Alberto; Olsen, Lars F.
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Water is involved in all aspects of biological activity, both as a solvent and as a reactant. It is hypothesized that intracellular water is in a highly structured state due to the high concentrations of macromolecules in the cell and that this may change the activity of intracellular enzymes due to altered binding affinities and allosteric regulations. Here we first investigate the kinetics of two glycolytic enzymes in artificially crowded aqueous solutions and show that crowding does indeed change their kinetics. Based on our kinetic measurements we propose a new model of oscillating glycolysis that instead of Michaelis-Menten or Monod-Wyman-Changeux kinetics uses the Yang-Ling adsorption isotherm introduced by G. Ling in the frame of the Association-Induction (AI) hypothesis. Using this model, we can reproduce previous experimental observations of the coupling of glycolytic oscillations and intracellular water dynamics, e.g., (i) during the metabolic oscillations, the latter variable oscillates in phase with ATP activity, and (ii) the emergence of glycolytic oscillations largely depends on the extent of intracellular water dipolar relaxation in cells in the resting state. Our results support the view that the extent of intracellular water dipolar relaxation is regulated by the ability of cytoplasmic proteins to polarize intracellular water with the assistance of ATP, as suggested in the AI hypothesis. This hypothesis may be relevant to the interpretation of many other biological oscillators, including cell signalling processes.
Fil: Thoke, Henrik S.. University Of Southern Denmark; Dinamarca
Fil: Bagatolli, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Olsen, Lars F.. University Of Southern Denmark; Dinamarca - Materia
-
oscillating glycolisis
intracellular water
molecular crowding
Yang-Ling isotherm - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/96877
Ver los metadatos del registro completo
id |
CONICETDig_b23b14b00d56b94837b524e79b43f84c |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/96877 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeastThoke, Henrik S.Bagatolli, Luis AlbertoOlsen, Lars F.oscillating glycolisisintracellular watermolecular crowdingYang-Ling isothermhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Water is involved in all aspects of biological activity, both as a solvent and as a reactant. It is hypothesized that intracellular water is in a highly structured state due to the high concentrations of macromolecules in the cell and that this may change the activity of intracellular enzymes due to altered binding affinities and allosteric regulations. Here we first investigate the kinetics of two glycolytic enzymes in artificially crowded aqueous solutions and show that crowding does indeed change their kinetics. Based on our kinetic measurements we propose a new model of oscillating glycolysis that instead of Michaelis-Menten or Monod-Wyman-Changeux kinetics uses the Yang-Ling adsorption isotherm introduced by G. Ling in the frame of the Association-Induction (AI) hypothesis. Using this model, we can reproduce previous experimental observations of the coupling of glycolytic oscillations and intracellular water dynamics, e.g., (i) during the metabolic oscillations, the latter variable oscillates in phase with ATP activity, and (ii) the emergence of glycolytic oscillations largely depends on the extent of intracellular water dipolar relaxation in cells in the resting state. Our results support the view that the extent of intracellular water dipolar relaxation is regulated by the ability of cytoplasmic proteins to polarize intracellular water with the assistance of ATP, as suggested in the AI hypothesis. This hypothesis may be relevant to the interpretation of many other biological oscillators, including cell signalling processes.Fil: Thoke, Henrik S.. University Of Southern Denmark; DinamarcaFil: Bagatolli, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Olsen, Lars F.. University Of Southern Denmark; DinamarcaRoyal Society of Chemistry2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96877Thoke, Henrik S.; Bagatolli, Luis Alberto; Olsen, Lars F.; Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast; Royal Society of Chemistry; Integrative Biology; 10; 10; 10-2018; 587-5971757-9694CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/C8IB00099Ainfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/ib/article/10/10/587/5261228info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2018/ib/c8ib00099ainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:15Zoai:ri.conicet.gov.ar:11336/96877instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:15.432CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast |
title |
Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast |
spellingShingle |
Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast Thoke, Henrik S. oscillating glycolisis intracellular water molecular crowding Yang-Ling isotherm |
title_short |
Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast |
title_full |
Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast |
title_fullStr |
Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast |
title_full_unstemmed |
Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast |
title_sort |
Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast |
dc.creator.none.fl_str_mv |
Thoke, Henrik S. Bagatolli, Luis Alberto Olsen, Lars F. |
author |
Thoke, Henrik S. |
author_facet |
Thoke, Henrik S. Bagatolli, Luis Alberto Olsen, Lars F. |
author_role |
author |
author2 |
Bagatolli, Luis Alberto Olsen, Lars F. |
author2_role |
author author |
dc.subject.none.fl_str_mv |
oscillating glycolisis intracellular water molecular crowding Yang-Ling isotherm |
topic |
oscillating glycolisis intracellular water molecular crowding Yang-Ling isotherm |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Water is involved in all aspects of biological activity, both as a solvent and as a reactant. It is hypothesized that intracellular water is in a highly structured state due to the high concentrations of macromolecules in the cell and that this may change the activity of intracellular enzymes due to altered binding affinities and allosteric regulations. Here we first investigate the kinetics of two glycolytic enzymes in artificially crowded aqueous solutions and show that crowding does indeed change their kinetics. Based on our kinetic measurements we propose a new model of oscillating glycolysis that instead of Michaelis-Menten or Monod-Wyman-Changeux kinetics uses the Yang-Ling adsorption isotherm introduced by G. Ling in the frame of the Association-Induction (AI) hypothesis. Using this model, we can reproduce previous experimental observations of the coupling of glycolytic oscillations and intracellular water dynamics, e.g., (i) during the metabolic oscillations, the latter variable oscillates in phase with ATP activity, and (ii) the emergence of glycolytic oscillations largely depends on the extent of intracellular water dipolar relaxation in cells in the resting state. Our results support the view that the extent of intracellular water dipolar relaxation is regulated by the ability of cytoplasmic proteins to polarize intracellular water with the assistance of ATP, as suggested in the AI hypothesis. This hypothesis may be relevant to the interpretation of many other biological oscillators, including cell signalling processes. Fil: Thoke, Henrik S.. University Of Southern Denmark; Dinamarca Fil: Bagatolli, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Olsen, Lars F.. University Of Southern Denmark; Dinamarca |
description |
Water is involved in all aspects of biological activity, both as a solvent and as a reactant. It is hypothesized that intracellular water is in a highly structured state due to the high concentrations of macromolecules in the cell and that this may change the activity of intracellular enzymes due to altered binding affinities and allosteric regulations. Here we first investigate the kinetics of two glycolytic enzymes in artificially crowded aqueous solutions and show that crowding does indeed change their kinetics. Based on our kinetic measurements we propose a new model of oscillating glycolysis that instead of Michaelis-Menten or Monod-Wyman-Changeux kinetics uses the Yang-Ling adsorption isotherm introduced by G. Ling in the frame of the Association-Induction (AI) hypothesis. Using this model, we can reproduce previous experimental observations of the coupling of glycolytic oscillations and intracellular water dynamics, e.g., (i) during the metabolic oscillations, the latter variable oscillates in phase with ATP activity, and (ii) the emergence of glycolytic oscillations largely depends on the extent of intracellular water dipolar relaxation in cells in the resting state. Our results support the view that the extent of intracellular water dipolar relaxation is regulated by the ability of cytoplasmic proteins to polarize intracellular water with the assistance of ATP, as suggested in the AI hypothesis. This hypothesis may be relevant to the interpretation of many other biological oscillators, including cell signalling processes. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/96877 Thoke, Henrik S.; Bagatolli, Luis Alberto; Olsen, Lars F.; Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast; Royal Society of Chemistry; Integrative Biology; 10; 10; 10-2018; 587-597 1757-9694 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/96877 |
identifier_str_mv |
Thoke, Henrik S.; Bagatolli, Luis Alberto; Olsen, Lars F.; Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast; Royal Society of Chemistry; Integrative Biology; 10; 10; 10-2018; 587-597 1757-9694 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1039/C8IB00099A info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/ib/article/10/10/587/5261228 info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2018/ib/c8ib00099a |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613648928997376 |
score |
13.070432 |