Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondii

Autores
Etcheverry, Miriam Graciela; Montemarani, Analía Mariana; Robledo, Sebastian Noel; Nesci, Andrea Verónica
Año de publicación
2018
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
This study pursued understanding the modes of action by which yeast isolates belonging to the complex Meyerozyma guilliermondii affect AFB1 production by Aspergillus flavus. Isolates Y16 and Y25 were not able to produce killer toxins. However, whole cells and some cellular constituents were able to reduce AFB1 concentrations in different evaluated conditions. Cell-free supernatant of isolates decreased AFB1 levels in liquid medium. For this reason, the effect on ground maize grains was studied. The characterization of the cell-free supernatants and the optimization of the biological decontamination activity of the toxin were tested. The experimental design showed that the best conditions for the cellfree supernatant of yeast Y16 to produce a significant reduction of AFB1 were 39° C, pH 8 and the presence of Mg+. The cell-free supernatant of yeast Y25 was effective at 28° C, pH 5 and presence of Mg+. These results made us suppose a possible enzymatic effect in the reduction of AFB1. To determine whether the effect of the cell-free supernatant of the yeasts on the accumulation of AFB1 was attributable to a protein activity, they were subjected to heat inactivation and proteinase k treatment. No changes on the reduction of the toxin levels were detected. The toxin reduction doesn’t correspond to an enzymatic effect. The cell-free supernatant from the two yeast isolates added to ground maize grains were able to reduce the levels of AFB1 when the toxin was already present in the substrate. Characterization of cell-free supernatants and optimization of the biological decontamination activity of the toxin are necessary steps in the process of selecting a treatment applicable to stored maize.
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montemarani, Analía Mariana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Robledo, Sebastian Noel. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nesci, Andrea Verónica. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
11th International Mycological Congress: Mycological Discoveries for a better world
San Juan de Puerto Rico
Puerto Rico
International Mycological Association
Mycological Society of America
Materia
Aflatoxin B1
Aspergillus flavus
Meyerozyma guilliermondii
Stored maize
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228171
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/228171
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondiiEtcheverry, Miriam GracielaMontemarani, Analía MarianaRobledo, Sebastian NoelNesci, Andrea VerónicaAflatoxin B1Aspergillus flavusMeyerozyma guilliermondiiStored maizehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study pursued understanding the modes of action by which yeast isolates belonging to the complex Meyerozyma guilliermondii affect AFB1 production by Aspergillus flavus. Isolates Y16 and Y25 were not able to produce killer toxins. However, whole cells and some cellular constituents were able to reduce AFB1 concentrations in different evaluated conditions. Cell-free supernatant of isolates decreased AFB1 levels in liquid medium. For this reason, the effect on ground maize grains was studied. The characterization of the cell-free supernatants and the optimization of the biological decontamination activity of the toxin were tested. The experimental design showed that the best conditions for the cellfree supernatant of yeast Y16 to produce a significant reduction of AFB1 were 39° C, pH 8 and the presence of Mg+. The cell-free supernatant of yeast Y25 was effective at 28° C, pH 5 and presence of Mg+. These results made us suppose a possible enzymatic effect in the reduction of AFB1. To determine whether the effect of the cell-free supernatant of the yeasts on the accumulation of AFB1 was attributable to a protein activity, they were subjected to heat inactivation and proteinase k treatment. No changes on the reduction of the toxin levels were detected. The toxin reduction doesn’t correspond to an enzymatic effect. The cell-free supernatant from the two yeast isolates added to ground maize grains were able to reduce the levels of AFB1 when the toxin was already present in the substrate. Characterization of cell-free supernatants and optimization of the biological decontamination activity of the toxin are necessary steps in the process of selecting a treatment applicable to stored maize.Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Montemarani, Analía Mariana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Robledo, Sebastian Noel. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nesci, Andrea Verónica. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina11th International Mycological Congress: Mycological Discoveries for a better worldSan Juan de Puerto RicoPuerto RicoInternational Mycological AssociationMycological Society of AmericaMycological Society of America2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/228171Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondii; 11th International Mycological Congress: Mycological Discoveries for a better world; San Juan de Puerto Rico; Puerto Rico; 2018; 372-372CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://www.britmycolsoc.org.uk/application/files/7215/3659/4574/IMC11_-_Programme_and_abstracts.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:31Zoai:ri.conicet.gov.ar:11336/228171instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:31.282CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondii
title Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondii
spellingShingle Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondii
Etcheverry, Miriam Graciela
Aflatoxin B1
Aspergillus flavus
Meyerozyma guilliermondii
Stored maize
title_short Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondii
title_full Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondii
title_fullStr Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondii
title_full_unstemmed Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondii
title_sort Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondii
dc.creator.none.fl_str_mv Etcheverry, Miriam Graciela
Montemarani, Analía Mariana
Robledo, Sebastian Noel
Nesci, Andrea Verónica
author Etcheverry, Miriam Graciela
author_facet Etcheverry, Miriam Graciela
Montemarani, Analía Mariana
Robledo, Sebastian Noel
Nesci, Andrea Verónica
author_role author
author2 Montemarani, Analía Mariana
Robledo, Sebastian Noel
Nesci, Andrea Verónica
author2_role author
author
author
dc.subject.none.fl_str_mv Aflatoxin B1
Aspergillus flavus
Meyerozyma guilliermondii
Stored maize
topic Aflatoxin B1
Aspergillus flavus
Meyerozyma guilliermondii
Stored maize
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study pursued understanding the modes of action by which yeast isolates belonging to the complex Meyerozyma guilliermondii affect AFB1 production by Aspergillus flavus. Isolates Y16 and Y25 were not able to produce killer toxins. However, whole cells and some cellular constituents were able to reduce AFB1 concentrations in different evaluated conditions. Cell-free supernatant of isolates decreased AFB1 levels in liquid medium. For this reason, the effect on ground maize grains was studied. The characterization of the cell-free supernatants and the optimization of the biological decontamination activity of the toxin were tested. The experimental design showed that the best conditions for the cellfree supernatant of yeast Y16 to produce a significant reduction of AFB1 were 39° C, pH 8 and the presence of Mg+. The cell-free supernatant of yeast Y25 was effective at 28° C, pH 5 and presence of Mg+. These results made us suppose a possible enzymatic effect in the reduction of AFB1. To determine whether the effect of the cell-free supernatant of the yeasts on the accumulation of AFB1 was attributable to a protein activity, they were subjected to heat inactivation and proteinase k treatment. No changes on the reduction of the toxin levels were detected. The toxin reduction doesn’t correspond to an enzymatic effect. The cell-free supernatant from the two yeast isolates added to ground maize grains were able to reduce the levels of AFB1 when the toxin was already present in the substrate. Characterization of cell-free supernatants and optimization of the biological decontamination activity of the toxin are necessary steps in the process of selecting a treatment applicable to stored maize.
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montemarani, Analía Mariana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Robledo, Sebastian Noel. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nesci, Andrea Verónica. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
11th International Mycological Congress: Mycological Discoveries for a better world
San Juan de Puerto Rico
Puerto Rico
International Mycological Association
Mycological Society of America
description This study pursued understanding the modes of action by which yeast isolates belonging to the complex Meyerozyma guilliermondii affect AFB1 production by Aspergillus flavus. Isolates Y16 and Y25 were not able to produce killer toxins. However, whole cells and some cellular constituents were able to reduce AFB1 concentrations in different evaluated conditions. Cell-free supernatant of isolates decreased AFB1 levels in liquid medium. For this reason, the effect on ground maize grains was studied. The characterization of the cell-free supernatants and the optimization of the biological decontamination activity of the toxin were tested. The experimental design showed that the best conditions for the cellfree supernatant of yeast Y16 to produce a significant reduction of AFB1 were 39° C, pH 8 and the presence of Mg+. The cell-free supernatant of yeast Y25 was effective at 28° C, pH 5 and presence of Mg+. These results made us suppose a possible enzymatic effect in the reduction of AFB1. To determine whether the effect of the cell-free supernatant of the yeasts on the accumulation of AFB1 was attributable to a protein activity, they were subjected to heat inactivation and proteinase k treatment. No changes on the reduction of the toxin levels were detected. The toxin reduction doesn’t correspond to an enzymatic effect. The cell-free supernatant from the two yeast isolates added to ground maize grains were able to reduce the levels of AFB1 when the toxin was already present in the substrate. Characterization of cell-free supernatants and optimization of the biological decontamination activity of the toxin are necessary steps in the process of selecting a treatment applicable to stored maize.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228171
Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondii; 11th International Mycological Congress: Mycological Discoveries for a better world; San Juan de Puerto Rico; Puerto Rico; 2018; 372-372
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228171
identifier_str_mv Decontamination of aflatoxin B1 and mycocinogenic activity evaluation by using yeasts belonging to the complex Meyerozyma guilliermondii; 11th International Mycological Congress: Mycological Discoveries for a better world; San Juan de Puerto Rico; Puerto Rico; 2018; 372-372
CONICET Digital
CONICET
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language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.britmycolsoc.org.uk/application/files/7215/3659/4574/IMC11_-_Programme_and_abstracts.pdf
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Mycological Society of America
publisher.none.fl_str_mv Mycological Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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