Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoides

Autores
Aredes Fernández, Pedro Adrián; Apud, Gisselle Raquel; Sampietro, Diego Alejandro
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Ochratoxin A (OTA) is a mycotoxin with harmful effects on human and animal health. Aspergillus section Nigri, mainly A. carbonarius and A. niger, are the major species producing OTA in grapes and wine. Special attention is being paid to OTA control in agricultural products because of health risks posed by contaminated food. In recent years, plant extracts and their metabolites have become popular to control mycotoxinproducing fungi. In previous assays, we demonstrated that the dichloromethane extract of Macfadyena cynanchoides stems and their antifungal constituents, identified as 2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthoquinone (lapachol) and 1-hydroxy4-methyl anthraquinone, show significant antifungal activity against A. niger and A. carbonarius. The aim of this work was to determine the antiochratoxigenic activity of the dichlorometane extract and their antifungal metabolites, alone and in combination with sodium metabisulfite in different sublethal concentrations. A volume of 4 mL grape juice was supplemented with each antifungal substance and inoculated with 5 × 103 spores/mL of A. carbonarius or A. niger, then incubated for six days at 15°C. At the end of incubation, the amount of OTA produced in the supernatant juice was determined by competitive ELISA using the Ridascreen-Fast ochratoxin A kit. The dichloromethane extract and its antifungal quinones produced a partial inhibition in the OTA production. Sodium metabisulfite induced OTA accumulation at the assayed concentrations. However, the natural antifungals completely suppressed OTA accumulation in mixtures with sodium metabisulfite at all concentrations assayed. We can conclude that the dichloromethane extract of M. cynanchoides and its antifungal molecules have potential use as a complement to sodium metabisulfite against the Aspergillus species responsible for black grape rots and OTA production.
Fil: Aredes Fernández, Pedro Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biotecnología; Argentina
Fil: Apud, Gisselle Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biotecnología; Argentina
Fil: Sampietro, Diego Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
70th American Society for Enology and Viticulture National Conference
Napa
Estados Unidos
American Society for Enology and Viticulture
Materia
ANTIOCHRATOXIGENIC ACTIVITY
ANTIFUNGAL METABOLITES
ASPERGILLUS
GRAPES
WINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281063
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/281063
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoidesAredes Fernández, Pedro AdriánApud, Gisselle RaquelSampietro, Diego AlejandroANTIOCHRATOXIGENIC ACTIVITYANTIFUNGAL METABOLITESASPERGILLUSGRAPESWINEhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Ochratoxin A (OTA) is a mycotoxin with harmful effects on human and animal health. Aspergillus section Nigri, mainly A. carbonarius and A. niger, are the major species producing OTA in grapes and wine. Special attention is being paid to OTA control in agricultural products because of health risks posed by contaminated food. In recent years, plant extracts and their metabolites have become popular to control mycotoxinproducing fungi. In previous assays, we demonstrated that the dichloromethane extract of Macfadyena cynanchoides stems and their antifungal constituents, identified as 2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthoquinone (lapachol) and 1-hydroxy4-methyl anthraquinone, show significant antifungal activity against A. niger and A. carbonarius. The aim of this work was to determine the antiochratoxigenic activity of the dichlorometane extract and their antifungal metabolites, alone and in combination with sodium metabisulfite in different sublethal concentrations. A volume of 4 mL grape juice was supplemented with each antifungal substance and inoculated with 5 × 103 spores/mL of A. carbonarius or A. niger, then incubated for six days at 15°C. At the end of incubation, the amount of OTA produced in the supernatant juice was determined by competitive ELISA using the Ridascreen-Fast ochratoxin A kit. The dichloromethane extract and its antifungal quinones produced a partial inhibition in the OTA production. Sodium metabisulfite induced OTA accumulation at the assayed concentrations. However, the natural antifungals completely suppressed OTA accumulation in mixtures with sodium metabisulfite at all concentrations assayed. We can conclude that the dichloromethane extract of M. cynanchoides and its antifungal molecules have potential use as a complement to sodium metabisulfite against the Aspergillus species responsible for black grape rots and OTA production.Fil: Aredes Fernández, Pedro Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biotecnología; ArgentinaFil: Apud, Gisselle Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biotecnología; ArgentinaFil: Sampietro, Diego Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina70th American Society for Enology and Viticulture National ConferenceNapaEstados UnidosAmerican Society for Enology and ViticultureAmerican Society for Enology and Viticulture2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281063Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoides; 70th American Society for Enology and Viticulture National Conference; Napa; Estados Unidos; 2019; 101-101CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.asev.org/wp-content/uploads/2024/09/2019technicalabstracts.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:06:59Zoai:ri.conicet.gov.ar:11336/281063instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:06:59.894CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoides
title Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoides
spellingShingle Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoides
Aredes Fernández, Pedro Adrián
ANTIOCHRATOXIGENIC ACTIVITY
ANTIFUNGAL METABOLITES
ASPERGILLUS
GRAPES
WINE
title_short Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoides
title_full Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoides
title_fullStr Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoides
title_full_unstemmed Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoides
title_sort Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoides
dc.creator.none.fl_str_mv Aredes Fernández, Pedro Adrián
Apud, Gisselle Raquel
Sampietro, Diego Alejandro
author Aredes Fernández, Pedro Adrián
author_facet Aredes Fernández, Pedro Adrián
Apud, Gisselle Raquel
Sampietro, Diego Alejandro
author_role author
author2 Apud, Gisselle Raquel
Sampietro, Diego Alejandro
author2_role author
author
dc.subject.none.fl_str_mv ANTIOCHRATOXIGENIC ACTIVITY
ANTIFUNGAL METABOLITES
ASPERGILLUS
GRAPES
WINE
topic ANTIOCHRATOXIGENIC ACTIVITY
ANTIFUNGAL METABOLITES
ASPERGILLUS
GRAPES
WINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Ochratoxin A (OTA) is a mycotoxin with harmful effects on human and animal health. Aspergillus section Nigri, mainly A. carbonarius and A. niger, are the major species producing OTA in grapes and wine. Special attention is being paid to OTA control in agricultural products because of health risks posed by contaminated food. In recent years, plant extracts and their metabolites have become popular to control mycotoxinproducing fungi. In previous assays, we demonstrated that the dichloromethane extract of Macfadyena cynanchoides stems and their antifungal constituents, identified as 2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthoquinone (lapachol) and 1-hydroxy4-methyl anthraquinone, show significant antifungal activity against A. niger and A. carbonarius. The aim of this work was to determine the antiochratoxigenic activity of the dichlorometane extract and their antifungal metabolites, alone and in combination with sodium metabisulfite in different sublethal concentrations. A volume of 4 mL grape juice was supplemented with each antifungal substance and inoculated with 5 × 103 spores/mL of A. carbonarius or A. niger, then incubated for six days at 15°C. At the end of incubation, the amount of OTA produced in the supernatant juice was determined by competitive ELISA using the Ridascreen-Fast ochratoxin A kit. The dichloromethane extract and its antifungal quinones produced a partial inhibition in the OTA production. Sodium metabisulfite induced OTA accumulation at the assayed concentrations. However, the natural antifungals completely suppressed OTA accumulation in mixtures with sodium metabisulfite at all concentrations assayed. We can conclude that the dichloromethane extract of M. cynanchoides and its antifungal molecules have potential use as a complement to sodium metabisulfite against the Aspergillus species responsible for black grape rots and OTA production.
Fil: Aredes Fernández, Pedro Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biotecnología; Argentina
Fil: Apud, Gisselle Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biotecnología; Argentina
Fil: Sampietro, Diego Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
70th American Society for Enology and Viticulture National Conference
Napa
Estados Unidos
American Society for Enology and Viticulture
description Ochratoxin A (OTA) is a mycotoxin with harmful effects on human and animal health. Aspergillus section Nigri, mainly A. carbonarius and A. niger, are the major species producing OTA in grapes and wine. Special attention is being paid to OTA control in agricultural products because of health risks posed by contaminated food. In recent years, plant extracts and their metabolites have become popular to control mycotoxinproducing fungi. In previous assays, we demonstrated that the dichloromethane extract of Macfadyena cynanchoides stems and their antifungal constituents, identified as 2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthoquinone (lapachol) and 1-hydroxy4-methyl anthraquinone, show significant antifungal activity against A. niger and A. carbonarius. The aim of this work was to determine the antiochratoxigenic activity of the dichlorometane extract and their antifungal metabolites, alone and in combination with sodium metabisulfite in different sublethal concentrations. A volume of 4 mL grape juice was supplemented with each antifungal substance and inoculated with 5 × 103 spores/mL of A. carbonarius or A. niger, then incubated for six days at 15°C. At the end of incubation, the amount of OTA produced in the supernatant juice was determined by competitive ELISA using the Ridascreen-Fast ochratoxin A kit. The dichloromethane extract and its antifungal quinones produced a partial inhibition in the OTA production. Sodium metabisulfite induced OTA accumulation at the assayed concentrations. However, the natural antifungals completely suppressed OTA accumulation in mixtures with sodium metabisulfite at all concentrations assayed. We can conclude that the dichloromethane extract of M. cynanchoides and its antifungal molecules have potential use as a complement to sodium metabisulfite against the Aspergillus species responsible for black grape rots and OTA production.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281063
Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoides; 70th American Society for Enology and Viticulture National Conference; Napa; Estados Unidos; 2019; 101-101
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281063
identifier_str_mv Antiochratoxigenic activity of antifungal metabolites from Macfadyena cynanchoides; 70th American Society for Enology and Viticulture National Conference; Napa; Estados Unidos; 2019; 101-101
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.asev.org/wp-content/uploads/2024/09/2019technicalabstracts.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv American Society for Enology and Viticulture
publisher.none.fl_str_mv American Society for Enology and Viticulture
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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