Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative study

Autores
Caravelli, Alejandro Horacio; Giannuzzi, Leda; Zaritzky, Noemi Elisabet
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chlorination is a very useful control method of filamentous bulking in activated sludge systems; however, it favours formation of undesirable compounds such as trihalomethanes. Other oxidants, such as ozone, could be used for bulking control. In view of the fact that chlorine and ozone are both non-selective chemical agents affecting filamentous and floc-forming micro-organisms, the determination of optimum dosage conditions becomes essential to minimise the impact produced on the activated sludge process. In this work, the effects of chlorine and ozone on the biomass concentration of activated sludge and on different parameters that characterise the microbial metabolic activity were compared. The following techniques were applied: Respirometry (oxygen uptake rate); and INT-dehydrogenase activity test carried out both by spectrophotometry (DHAa) and image analysis (DHAi). The respirometric technique and the DHAa test quantified oxidants action on the total respiratory activity of flocs; the image DHAi test was applied to evaluate the specific action of the oxidants on filamentous micro-organisms. Additionally, plate count technique, respirometry and DHAa test were correlated using chlorine and ozone experiments on pure cultures of a floc-forming micro-organism (Acinetobacter anitratus) to compare the effect of the oxidising agents on the metabolic activity and the viability of the micro-organisms. Ozone was found to have more intense antimicrobial action. In activated sludge, ozone reduced total biomass concentration by oxidising various components and causing cell lysis. An equation was proposed to estimate biomass concentration of activated sludge as a function of time and ozone dose rate; in contrast, at the doses applied, chlorine did not reduce the concentration of activated sludge biomass. In activated sludge, adequate conditions for both oxidants were identified under which the respiratory activity of filamentous micro-organisms could be considerably inhibited, causing the lowest possible impact on whole floc metabolic activity. An initial chlorine dose of 7.9 mgCl2·gVSS-1 for a contact time of 5 min (initial pulse= 6.0 mgCl2·ℓ-1), and a total ozone dose of 66.0 mgO3·gVSS-1 (ozone dose rate of 3.3 mgO3·gVSS-1·min-1 for a contact time of 20 min) were the most suitable conditions to control filamentous bulking.
Fil: Caravelli, Alejandro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Giannuzzi, Leda. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina
Materia
Filamentous bulking
Chlorine, ozone
INT-dehydrogenase activity
Respirometry
Image analysis
Plate count
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/115898
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/115898
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative studyCaravelli, Alejandro HoracioGiannuzzi, LedaZaritzky, Noemi ElisabetFilamentous bulkingChlorine, ozoneINT-dehydrogenase activityRespirometryImage analysisPlate counthttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Chlorination is a very useful control method of filamentous bulking in activated sludge systems; however, it favours formation of undesirable compounds such as trihalomethanes. Other oxidants, such as ozone, could be used for bulking control. In view of the fact that chlorine and ozone are both non-selective chemical agents affecting filamentous and floc-forming micro-organisms, the determination of optimum dosage conditions becomes essential to minimise the impact produced on the activated sludge process. In this work, the effects of chlorine and ozone on the biomass concentration of activated sludge and on different parameters that characterise the microbial metabolic activity were compared. The following techniques were applied: Respirometry (oxygen uptake rate); and INT-dehydrogenase activity test carried out both by spectrophotometry (DHAa) and image analysis (DHAi). The respirometric technique and the DHAa test quantified oxidants action on the total respiratory activity of flocs; the image DHAi test was applied to evaluate the specific action of the oxidants on filamentous micro-organisms. Additionally, plate count technique, respirometry and DHAa test were correlated using chlorine and ozone experiments on pure cultures of a floc-forming micro-organism (Acinetobacter anitratus) to compare the effect of the oxidising agents on the metabolic activity and the viability of the micro-organisms. Ozone was found to have more intense antimicrobial action. In activated sludge, ozone reduced total biomass concentration by oxidising various components and causing cell lysis. An equation was proposed to estimate biomass concentration of activated sludge as a function of time and ozone dose rate; in contrast, at the doses applied, chlorine did not reduce the concentration of activated sludge biomass. In activated sludge, adequate conditions for both oxidants were identified under which the respiratory activity of filamentous micro-organisms could be considerably inhibited, causing the lowest possible impact on whole floc metabolic activity. An initial chlorine dose of 7.9 mgCl2·gVSS-1 for a contact time of 5 min (initial pulse= 6.0 mgCl2·ℓ-1), and a total ozone dose of 66.0 mgO3·gVSS-1 (ozone dose rate of 3.3 mgO3·gVSS-1·min-1 for a contact time of 20 min) were the most suitable conditions to control filamentous bulking.Fil: Caravelli, Alejandro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Giannuzzi, Leda. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; ArgentinaWater Research Commission2007-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/115898Caravelli, Alejandro Horacio; Giannuzzi, Leda; Zaritzky, Noemi Elisabet; Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative study; Water Research Commission; Water S.a.; 32; 4; 12-2007; 585-5950378-47381816-7950CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4314/wsa.v32i4.5283info:eu-repo/semantics/altIdentifier/url/https://tinyurl.com/y4kq9zcfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:03:01Zoai:ri.conicet.gov.ar:11336/115898instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:03:01.898CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative study
title Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative study
spellingShingle Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative study
Caravelli, Alejandro Horacio
Filamentous bulking
Chlorine, ozone
INT-dehydrogenase activity
Respirometry
Image analysis
Plate count
title_short Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative study
title_full Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative study
title_fullStr Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative study
title_full_unstemmed Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative study
title_sort Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative study
dc.creator.none.fl_str_mv Caravelli, Alejandro Horacio
Giannuzzi, Leda
Zaritzky, Noemi Elisabet
author Caravelli, Alejandro Horacio
author_facet Caravelli, Alejandro Horacio
Giannuzzi, Leda
Zaritzky, Noemi Elisabet
author_role author
author2 Giannuzzi, Leda
Zaritzky, Noemi Elisabet
author2_role author
author
dc.subject.none.fl_str_mv Filamentous bulking
Chlorine, ozone
INT-dehydrogenase activity
Respirometry
Image analysis
Plate count
topic Filamentous bulking
Chlorine, ozone
INT-dehydrogenase activity
Respirometry
Image analysis
Plate count
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Chlorination is a very useful control method of filamentous bulking in activated sludge systems; however, it favours formation of undesirable compounds such as trihalomethanes. Other oxidants, such as ozone, could be used for bulking control. In view of the fact that chlorine and ozone are both non-selective chemical agents affecting filamentous and floc-forming micro-organisms, the determination of optimum dosage conditions becomes essential to minimise the impact produced on the activated sludge process. In this work, the effects of chlorine and ozone on the biomass concentration of activated sludge and on different parameters that characterise the microbial metabolic activity were compared. The following techniques were applied: Respirometry (oxygen uptake rate); and INT-dehydrogenase activity test carried out both by spectrophotometry (DHAa) and image analysis (DHAi). The respirometric technique and the DHAa test quantified oxidants action on the total respiratory activity of flocs; the image DHAi test was applied to evaluate the specific action of the oxidants on filamentous micro-organisms. Additionally, plate count technique, respirometry and DHAa test were correlated using chlorine and ozone experiments on pure cultures of a floc-forming micro-organism (Acinetobacter anitratus) to compare the effect of the oxidising agents on the metabolic activity and the viability of the micro-organisms. Ozone was found to have more intense antimicrobial action. In activated sludge, ozone reduced total biomass concentration by oxidising various components and causing cell lysis. An equation was proposed to estimate biomass concentration of activated sludge as a function of time and ozone dose rate; in contrast, at the doses applied, chlorine did not reduce the concentration of activated sludge biomass. In activated sludge, adequate conditions for both oxidants were identified under which the respiratory activity of filamentous micro-organisms could be considerably inhibited, causing the lowest possible impact on whole floc metabolic activity. An initial chlorine dose of 7.9 mgCl2·gVSS-1 for a contact time of 5 min (initial pulse= 6.0 mgCl2·ℓ-1), and a total ozone dose of 66.0 mgO3·gVSS-1 (ozone dose rate of 3.3 mgO3·gVSS-1·min-1 for a contact time of 20 min) were the most suitable conditions to control filamentous bulking.
Fil: Caravelli, Alejandro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Giannuzzi, Leda. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina
description Chlorination is a very useful control method of filamentous bulking in activated sludge systems; however, it favours formation of undesirable compounds such as trihalomethanes. Other oxidants, such as ozone, could be used for bulking control. In view of the fact that chlorine and ozone are both non-selective chemical agents affecting filamentous and floc-forming micro-organisms, the determination of optimum dosage conditions becomes essential to minimise the impact produced on the activated sludge process. In this work, the effects of chlorine and ozone on the biomass concentration of activated sludge and on different parameters that characterise the microbial metabolic activity were compared. The following techniques were applied: Respirometry (oxygen uptake rate); and INT-dehydrogenase activity test carried out both by spectrophotometry (DHAa) and image analysis (DHAi). The respirometric technique and the DHAa test quantified oxidants action on the total respiratory activity of flocs; the image DHAi test was applied to evaluate the specific action of the oxidants on filamentous micro-organisms. Additionally, plate count technique, respirometry and DHAa test were correlated using chlorine and ozone experiments on pure cultures of a floc-forming micro-organism (Acinetobacter anitratus) to compare the effect of the oxidising agents on the metabolic activity and the viability of the micro-organisms. Ozone was found to have more intense antimicrobial action. In activated sludge, ozone reduced total biomass concentration by oxidising various components and causing cell lysis. An equation was proposed to estimate biomass concentration of activated sludge as a function of time and ozone dose rate; in contrast, at the doses applied, chlorine did not reduce the concentration of activated sludge biomass. In activated sludge, adequate conditions for both oxidants were identified under which the respiratory activity of filamentous micro-organisms could be considerably inhibited, causing the lowest possible impact on whole floc metabolic activity. An initial chlorine dose of 7.9 mgCl2·gVSS-1 for a contact time of 5 min (initial pulse= 6.0 mgCl2·ℓ-1), and a total ozone dose of 66.0 mgO3·gVSS-1 (ozone dose rate of 3.3 mgO3·gVSS-1·min-1 for a contact time of 20 min) were the most suitable conditions to control filamentous bulking.
publishDate 2007
dc.date.none.fl_str_mv 2007-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/115898
Caravelli, Alejandro Horacio; Giannuzzi, Leda; Zaritzky, Noemi Elisabet; Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative study; Water Research Commission; Water S.a.; 32; 4; 12-2007; 585-595
0378-4738
1816-7950
CONICET Digital
CONICET
url http://hdl.handle.net/11336/115898
identifier_str_mv Caravelli, Alejandro Horacio; Giannuzzi, Leda; Zaritzky, Noemi Elisabet; Effectiveness of chlorination and ozonation methods on pure cultures of floc-forming micro-organisms and activated sludge: A comparative study; Water Research Commission; Water S.a.; 32; 4; 12-2007; 585-595
0378-4738
1816-7950
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4314/wsa.v32i4.5283
info:eu-repo/semantics/altIdentifier/url/https://tinyurl.com/y4kq9zcf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Water Research Commission
publisher.none.fl_str_mv Water Research Commission
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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