Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges

Autores
Caravelli, Alejandro Horacio; Contreras, Edgardo Martin; Giannuzzi, Leda; Zaritzky, Noemi Elisabet
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Activated sludge technology is more used than any other for biological treatment of wastewater. However, filamentous bulking is a very common problem in activatedslud ge plants, chlorine being the chemical agent normally used to control it. In this work the effect of chlorine on microorganisms present in activatedslud ge flocs was assessed by a respirometric technique (oxygen uptake rate, OUR) and by the INT-dehydrogenase activity test (DHA) measured by two techniques: spectrophotometry (DHAa) andimage analysis (DHAi). Both DHA tests were optimized and correlated with the respirometric technique (OUR) using pure cultures of a filamentous microorganism (Sphaerotilus natans) under chlorine inhibition. Using these correlations the tested methods were applied to determine the action of chlorine on respiratory activity in activatedslud ge. The OUR andthe DHAa quantifies the action of chlorine on the total respiratory activity (RA) of flocs (filamentous andfloc-forming bacteria); in contrast, the DHAi test evaluatesspecific action of chlorine on the RA of filamentous microorganisms.In activated sludge flocs containing filamentous microorganisms, a chlorine dose of 4.75mg Cl2 (gVSS)1 with a contact time of 20 min reduced about 80% of the RA of filamentous bacteria while affecting only 50-60% of the total RA of flocs. Besides, a chlorine dose of 7.9 mgCl2 (gVSS)1 produced the total respiratory inactivation of filamentous microorganisms after 10 min contact, however, with this dose the total RA of activated sludge flocs was reduced only about 45–65%; controlling filamentous bulking without affecting too much floc-forming bacteria.At the testedchlorine concentrations the inhibition of filamentous microorganisms was higher than in the whole activated sludge. Although floc-forming microorganisms were demonstrated to be more susceptible to chlorine than filamentous in pure cultures, results obtainedin the present work confirmed that it is the location of the filamentous microorganisms in the flocs and the presence of extracellular polymer substances which largely determines their higher susceptibility to chlorine; consequently this feature plays a critical role in bulking control.
Fil: Caravelli, Alejandro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Contreras, Edgardo Martin. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Giannuzzi, Leda. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Materia
S.natans
filamentous microorganisms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/127137
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/127137
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludgesCaravelli, Alejandro HoracioContreras, Edgardo MartinGiannuzzi, LedaZaritzky, Noemi ElisabetS.natansfilamentous microorganismshttps://purl.org/becyt/ford/2.7https://purl.org/becyt/ford/2Activated sludge technology is more used than any other for biological treatment of wastewater. However, filamentous bulking is a very common problem in activatedslud ge plants, chlorine being the chemical agent normally used to control it. In this work the effect of chlorine on microorganisms present in activatedslud ge flocs was assessed by a respirometric technique (oxygen uptake rate, OUR) and by the INT-dehydrogenase activity test (DHA) measured by two techniques: spectrophotometry (DHAa) andimage analysis (DHAi). Both DHA tests were optimized and correlated with the respirometric technique (OUR) using pure cultures of a filamentous microorganism (Sphaerotilus natans) under chlorine inhibition. Using these correlations the tested methods were applied to determine the action of chlorine on respiratory activity in activatedslud ge. The OUR andthe DHAa quantifies the action of chlorine on the total respiratory activity (RA) of flocs (filamentous andfloc-forming bacteria); in contrast, the DHAi test evaluatesspecific action of chlorine on the RA of filamentous microorganisms.In activated sludge flocs containing filamentous microorganisms, a chlorine dose of 4.75mg Cl2 (gVSS)1 with a contact time of 20 min reduced about 80% of the RA of filamentous bacteria while affecting only 50-60% of the total RA of flocs. Besides, a chlorine dose of 7.9 mgCl2 (gVSS)1 produced the total respiratory inactivation of filamentous microorganisms after 10 min contact, however, with this dose the total RA of activated sludge flocs was reduced only about 45–65%; controlling filamentous bulking without affecting too much floc-forming bacteria.At the testedchlorine concentrations the inhibition of filamentous microorganisms was higher than in the whole activated sludge. Although floc-forming microorganisms were demonstrated to be more susceptible to chlorine than filamentous in pure cultures, results obtainedin the present work confirmed that it is the location of the filamentous microorganisms in the flocs and the presence of extracellular polymer substances which largely determines their higher susceptibility to chlorine; consequently this feature plays a critical role in bulking control.Fil: Caravelli, Alejandro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Contreras, Edgardo Martin. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Giannuzzi, Leda. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaPergamon-Elsevier Science Ltd2003-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/127137Caravelli, Alejandro Horacio; Contreras, Edgardo Martin; Giannuzzi, Leda; Zaritzky, Noemi Elisabet; Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges; Pergamon-Elsevier Science Ltd; Water Research; 37; 3-2003; 2097-21050043-1354CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0043135402006012info:eu-repo/semantics/altIdentifier/doi/10.1016/S0043-1354(02)00601-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:41:57Zoai:ri.conicet.gov.ar:11336/127137instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:41:58.165CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges
title Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges
spellingShingle Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges
Caravelli, Alejandro Horacio
S.natans
filamentous microorganisms
title_short Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges
title_full Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges
title_fullStr Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges
title_full_unstemmed Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges
title_sort Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges
dc.creator.none.fl_str_mv Caravelli, Alejandro Horacio
Contreras, Edgardo Martin
Giannuzzi, Leda
Zaritzky, Noemi Elisabet
author Caravelli, Alejandro Horacio
author_facet Caravelli, Alejandro Horacio
Contreras, Edgardo Martin
Giannuzzi, Leda
Zaritzky, Noemi Elisabet
author_role author
author2 Contreras, Edgardo Martin
Giannuzzi, Leda
Zaritzky, Noemi Elisabet
author2_role author
author
author
dc.subject.none.fl_str_mv S.natans
filamentous microorganisms
topic S.natans
filamentous microorganisms
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.7
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Activated sludge technology is more used than any other for biological treatment of wastewater. However, filamentous bulking is a very common problem in activatedslud ge plants, chlorine being the chemical agent normally used to control it. In this work the effect of chlorine on microorganisms present in activatedslud ge flocs was assessed by a respirometric technique (oxygen uptake rate, OUR) and by the INT-dehydrogenase activity test (DHA) measured by two techniques: spectrophotometry (DHAa) andimage analysis (DHAi). Both DHA tests were optimized and correlated with the respirometric technique (OUR) using pure cultures of a filamentous microorganism (Sphaerotilus natans) under chlorine inhibition. Using these correlations the tested methods were applied to determine the action of chlorine on respiratory activity in activatedslud ge. The OUR andthe DHAa quantifies the action of chlorine on the total respiratory activity (RA) of flocs (filamentous andfloc-forming bacteria); in contrast, the DHAi test evaluatesspecific action of chlorine on the RA of filamentous microorganisms.In activated sludge flocs containing filamentous microorganisms, a chlorine dose of 4.75mg Cl2 (gVSS)1 with a contact time of 20 min reduced about 80% of the RA of filamentous bacteria while affecting only 50-60% of the total RA of flocs. Besides, a chlorine dose of 7.9 mgCl2 (gVSS)1 produced the total respiratory inactivation of filamentous microorganisms after 10 min contact, however, with this dose the total RA of activated sludge flocs was reduced only about 45–65%; controlling filamentous bulking without affecting too much floc-forming bacteria.At the testedchlorine concentrations the inhibition of filamentous microorganisms was higher than in the whole activated sludge. Although floc-forming microorganisms were demonstrated to be more susceptible to chlorine than filamentous in pure cultures, results obtainedin the present work confirmed that it is the location of the filamentous microorganisms in the flocs and the presence of extracellular polymer substances which largely determines their higher susceptibility to chlorine; consequently this feature plays a critical role in bulking control.
Fil: Caravelli, Alejandro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Contreras, Edgardo Martin. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Giannuzzi, Leda. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
description Activated sludge technology is more used than any other for biological treatment of wastewater. However, filamentous bulking is a very common problem in activatedslud ge plants, chlorine being the chemical agent normally used to control it. In this work the effect of chlorine on microorganisms present in activatedslud ge flocs was assessed by a respirometric technique (oxygen uptake rate, OUR) and by the INT-dehydrogenase activity test (DHA) measured by two techniques: spectrophotometry (DHAa) andimage analysis (DHAi). Both DHA tests were optimized and correlated with the respirometric technique (OUR) using pure cultures of a filamentous microorganism (Sphaerotilus natans) under chlorine inhibition. Using these correlations the tested methods were applied to determine the action of chlorine on respiratory activity in activatedslud ge. The OUR andthe DHAa quantifies the action of chlorine on the total respiratory activity (RA) of flocs (filamentous andfloc-forming bacteria); in contrast, the DHAi test evaluatesspecific action of chlorine on the RA of filamentous microorganisms.In activated sludge flocs containing filamentous microorganisms, a chlorine dose of 4.75mg Cl2 (gVSS)1 with a contact time of 20 min reduced about 80% of the RA of filamentous bacteria while affecting only 50-60% of the total RA of flocs. Besides, a chlorine dose of 7.9 mgCl2 (gVSS)1 produced the total respiratory inactivation of filamentous microorganisms after 10 min contact, however, with this dose the total RA of activated sludge flocs was reduced only about 45–65%; controlling filamentous bulking without affecting too much floc-forming bacteria.At the testedchlorine concentrations the inhibition of filamentous microorganisms was higher than in the whole activated sludge. Although floc-forming microorganisms were demonstrated to be more susceptible to chlorine than filamentous in pure cultures, results obtainedin the present work confirmed that it is the location of the filamentous microorganisms in the flocs and the presence of extracellular polymer substances which largely determines their higher susceptibility to chlorine; consequently this feature plays a critical role in bulking control.
publishDate 2003
dc.date.none.fl_str_mv 2003-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/127137
Caravelli, Alejandro Horacio; Contreras, Edgardo Martin; Giannuzzi, Leda; Zaritzky, Noemi Elisabet; Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges; Pergamon-Elsevier Science Ltd; Water Research; 37; 3-2003; 2097-2105
0043-1354
CONICET Digital
CONICET
url http://hdl.handle.net/11336/127137
identifier_str_mv Caravelli, Alejandro Horacio; Contreras, Edgardo Martin; Giannuzzi, Leda; Zaritzky, Noemi Elisabet; Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges; Pergamon-Elsevier Science Ltd; Water Research; 37; 3-2003; 2097-2105
0043-1354
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0043135402006012
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0043-1354(02)00601-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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