Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges
- Autores
- Caravelli, Alejandro Horacio; Contreras, Edgardo Martin; Giannuzzi, Leda; Zaritzky, Noemi Elisabet
- Año de publicación
- 2003
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Activated sludge technology is more used than any other for biological treatment of wastewater. However, filamentous bulking is a very common problem in activatedslud ge plants, chlorine being the chemical agent normally used to control it. In this work the effect of chlorine on microorganisms present in activatedslud ge flocs was assessed by a respirometric technique (oxygen uptake rate, OUR) and by the INT-dehydrogenase activity test (DHA) measured by two techniques: spectrophotometry (DHAa) andimage analysis (DHAi). Both DHA tests were optimized and correlated with the respirometric technique (OUR) using pure cultures of a filamentous microorganism (Sphaerotilus natans) under chlorine inhibition. Using these correlations the tested methods were applied to determine the action of chlorine on respiratory activity in activatedslud ge. The OUR andthe DHAa quantifies the action of chlorine on the total respiratory activity (RA) of flocs (filamentous andfloc-forming bacteria); in contrast, the DHAi test evaluatesspecific action of chlorine on the RA of filamentous microorganisms.In activated sludge flocs containing filamentous microorganisms, a chlorine dose of 4.75mg Cl2 (gVSS)1 with a contact time of 20 min reduced about 80% of the RA of filamentous bacteria while affecting only 50-60% of the total RA of flocs. Besides, a chlorine dose of 7.9 mgCl2 (gVSS)1 produced the total respiratory inactivation of filamentous microorganisms after 10 min contact, however, with this dose the total RA of activated sludge flocs was reduced only about 45–65%; controlling filamentous bulking without affecting too much floc-forming bacteria.At the testedchlorine concentrations the inhibition of filamentous microorganisms was higher than in the whole activated sludge. Although floc-forming microorganisms were demonstrated to be more susceptible to chlorine than filamentous in pure cultures, results obtainedin the present work confirmed that it is the location of the filamentous microorganisms in the flocs and the presence of extracellular polymer substances which largely determines their higher susceptibility to chlorine; consequently this feature plays a critical role in bulking control.
Fil: Caravelli, Alejandro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Contreras, Edgardo Martin. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Giannuzzi, Leda. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina - Materia
-
S.natans
filamentous microorganisms - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/127137
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Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludgesCaravelli, Alejandro HoracioContreras, Edgardo MartinGiannuzzi, LedaZaritzky, Noemi ElisabetS.natansfilamentous microorganismshttps://purl.org/becyt/ford/2.7https://purl.org/becyt/ford/2Activated sludge technology is more used than any other for biological treatment of wastewater. However, filamentous bulking is a very common problem in activatedslud ge plants, chlorine being the chemical agent normally used to control it. In this work the effect of chlorine on microorganisms present in activatedslud ge flocs was assessed by a respirometric technique (oxygen uptake rate, OUR) and by the INT-dehydrogenase activity test (DHA) measured by two techniques: spectrophotometry (DHAa) andimage analysis (DHAi). Both DHA tests were optimized and correlated with the respirometric technique (OUR) using pure cultures of a filamentous microorganism (Sphaerotilus natans) under chlorine inhibition. Using these correlations the tested methods were applied to determine the action of chlorine on respiratory activity in activatedslud ge. The OUR andthe DHAa quantifies the action of chlorine on the total respiratory activity (RA) of flocs (filamentous andfloc-forming bacteria); in contrast, the DHAi test evaluatesspecific action of chlorine on the RA of filamentous microorganisms.In activated sludge flocs containing filamentous microorganisms, a chlorine dose of 4.75mg Cl2 (gVSS)1 with a contact time of 20 min reduced about 80% of the RA of filamentous bacteria while affecting only 50-60% of the total RA of flocs. Besides, a chlorine dose of 7.9 mgCl2 (gVSS)1 produced the total respiratory inactivation of filamentous microorganisms after 10 min contact, however, with this dose the total RA of activated sludge flocs was reduced only about 45–65%; controlling filamentous bulking without affecting too much floc-forming bacteria.At the testedchlorine concentrations the inhibition of filamentous microorganisms was higher than in the whole activated sludge. Although floc-forming microorganisms were demonstrated to be more susceptible to chlorine than filamentous in pure cultures, results obtainedin the present work confirmed that it is the location of the filamentous microorganisms in the flocs and the presence of extracellular polymer substances which largely determines their higher susceptibility to chlorine; consequently this feature plays a critical role in bulking control.Fil: Caravelli, Alejandro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Contreras, Edgardo Martin. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Giannuzzi, Leda. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaPergamon-Elsevier Science Ltd2003-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/127137Caravelli, Alejandro Horacio; Contreras, Edgardo Martin; Giannuzzi, Leda; Zaritzky, Noemi Elisabet; Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges; Pergamon-Elsevier Science Ltd; Water Research; 37; 3-2003; 2097-21050043-1354CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0043135402006012info:eu-repo/semantics/altIdentifier/doi/10.1016/S0043-1354(02)00601-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:04Zoai:ri.conicet.gov.ar:11336/127137instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:04.88CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges |
title |
Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges |
spellingShingle |
Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges Caravelli, Alejandro Horacio S.natans filamentous microorganisms |
title_short |
Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges |
title_full |
Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges |
title_fullStr |
Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges |
title_full_unstemmed |
Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges |
title_sort |
Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges |
dc.creator.none.fl_str_mv |
Caravelli, Alejandro Horacio Contreras, Edgardo Martin Giannuzzi, Leda Zaritzky, Noemi Elisabet |
author |
Caravelli, Alejandro Horacio |
author_facet |
Caravelli, Alejandro Horacio Contreras, Edgardo Martin Giannuzzi, Leda Zaritzky, Noemi Elisabet |
author_role |
author |
author2 |
Contreras, Edgardo Martin Giannuzzi, Leda Zaritzky, Noemi Elisabet |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
S.natans filamentous microorganisms |
topic |
S.natans filamentous microorganisms |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.7 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Activated sludge technology is more used than any other for biological treatment of wastewater. However, filamentous bulking is a very common problem in activatedslud ge plants, chlorine being the chemical agent normally used to control it. In this work the effect of chlorine on microorganisms present in activatedslud ge flocs was assessed by a respirometric technique (oxygen uptake rate, OUR) and by the INT-dehydrogenase activity test (DHA) measured by two techniques: spectrophotometry (DHAa) andimage analysis (DHAi). Both DHA tests were optimized and correlated with the respirometric technique (OUR) using pure cultures of a filamentous microorganism (Sphaerotilus natans) under chlorine inhibition. Using these correlations the tested methods were applied to determine the action of chlorine on respiratory activity in activatedslud ge. The OUR andthe DHAa quantifies the action of chlorine on the total respiratory activity (RA) of flocs (filamentous andfloc-forming bacteria); in contrast, the DHAi test evaluatesspecific action of chlorine on the RA of filamentous microorganisms.In activated sludge flocs containing filamentous microorganisms, a chlorine dose of 4.75mg Cl2 (gVSS)1 with a contact time of 20 min reduced about 80% of the RA of filamentous bacteria while affecting only 50-60% of the total RA of flocs. Besides, a chlorine dose of 7.9 mgCl2 (gVSS)1 produced the total respiratory inactivation of filamentous microorganisms after 10 min contact, however, with this dose the total RA of activated sludge flocs was reduced only about 45–65%; controlling filamentous bulking without affecting too much floc-forming bacteria.At the testedchlorine concentrations the inhibition of filamentous microorganisms was higher than in the whole activated sludge. Although floc-forming microorganisms were demonstrated to be more susceptible to chlorine than filamentous in pure cultures, results obtainedin the present work confirmed that it is the location of the filamentous microorganisms in the flocs and the presence of extracellular polymer substances which largely determines their higher susceptibility to chlorine; consequently this feature plays a critical role in bulking control. Fil: Caravelli, Alejandro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Contreras, Edgardo Martin. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Giannuzzi, Leda. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina |
description |
Activated sludge technology is more used than any other for biological treatment of wastewater. However, filamentous bulking is a very common problem in activatedslud ge plants, chlorine being the chemical agent normally used to control it. In this work the effect of chlorine on microorganisms present in activatedslud ge flocs was assessed by a respirometric technique (oxygen uptake rate, OUR) and by the INT-dehydrogenase activity test (DHA) measured by two techniques: spectrophotometry (DHAa) andimage analysis (DHAi). Both DHA tests were optimized and correlated with the respirometric technique (OUR) using pure cultures of a filamentous microorganism (Sphaerotilus natans) under chlorine inhibition. Using these correlations the tested methods were applied to determine the action of chlorine on respiratory activity in activatedslud ge. The OUR andthe DHAa quantifies the action of chlorine on the total respiratory activity (RA) of flocs (filamentous andfloc-forming bacteria); in contrast, the DHAi test evaluatesspecific action of chlorine on the RA of filamentous microorganisms.In activated sludge flocs containing filamentous microorganisms, a chlorine dose of 4.75mg Cl2 (gVSS)1 with a contact time of 20 min reduced about 80% of the RA of filamentous bacteria while affecting only 50-60% of the total RA of flocs. Besides, a chlorine dose of 7.9 mgCl2 (gVSS)1 produced the total respiratory inactivation of filamentous microorganisms after 10 min contact, however, with this dose the total RA of activated sludge flocs was reduced only about 45–65%; controlling filamentous bulking without affecting too much floc-forming bacteria.At the testedchlorine concentrations the inhibition of filamentous microorganisms was higher than in the whole activated sludge. Although floc-forming microorganisms were demonstrated to be more susceptible to chlorine than filamentous in pure cultures, results obtainedin the present work confirmed that it is the location of the filamentous microorganisms in the flocs and the presence of extracellular polymer substances which largely determines their higher susceptibility to chlorine; consequently this feature plays a critical role in bulking control. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/127137 Caravelli, Alejandro Horacio; Contreras, Edgardo Martin; Giannuzzi, Leda; Zaritzky, Noemi Elisabet; Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges; Pergamon-Elsevier Science Ltd; Water Research; 37; 3-2003; 2097-2105 0043-1354 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/127137 |
identifier_str_mv |
Caravelli, Alejandro Horacio; Contreras, Edgardo Martin; Giannuzzi, Leda; Zaritzky, Noemi Elisabet; Modelling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges; Pergamon-Elsevier Science Ltd; Water Research; 37; 3-2003; 2097-2105 0043-1354 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0043135402006012 info:eu-repo/semantics/altIdentifier/doi/10.1016/S0043-1354(02)00601-2 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613495254941696 |
score |
13.070432 |