Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles

Autores
Hamer, Mariana; Carballo, Romina Raquel; Rezzano, Irene Noemí
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The covalent binding of the porphyrin to immobilized gold nanoparticles resulted in a very useful and simple method to evaluate the absorption spectroscopy of a monolayer of porphyrins and the spectral changes produced by additional ligands. The layer by layer deposition showed no distinguishable porphyrin-related absorption bands whereas the direct simultaneous binding of porphyrins (Cu(II) and Fe(III) tetrapyridylporphine) to immobilized gold nanoparticles showed a significantly higher absorption band at 412 nm corresponding to the Soret band of the porphyrin ring. The ATR-FTIR spectra showed typical absorptions bands of porphyrin core at around 1000 cm−1 and 800 cm−1, which were only observed in the Cu(II) complex of TPyP/AuNPs–APTES/SiO2 suggesting that the CuTPyP molecule is tilted with respect to the substrate surface. The presence of FeTPyP produces changes in the geometry of the adsorbed CuTPyP porphyrins, indicating a flat accommodation of the porphyrinic rings in FeTPyP–CuTPyP composite that could be the reason of the differential tendency to include H2O2 as ligand. The difference UV–visible spectra of the bimetallic structures showed a very sensitive response to H2O2 concentration with a Kapp 3.75 × 103 M−1(R: 0.9865; Std E: 2.6 × 10−2) which was notoriously similar to Km value for peroxidase enzyme (2.5 × 103 M−1).
Fil: Hamer, Mariana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carballo, Romina Raquel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rezzano, Irene Noemí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Gold Nanoparticles
Heterobimetallic Porphyrin Complexes
Enzyme Mimics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18169

id CONICETDig_b0b2947d1146aa2d168543ff98bdfb0a
oai_identifier_str oai:ri.conicet.gov.ar:11336/18169
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticlesHamer, MarianaCarballo, Romina RaquelRezzano, Irene NoemíGold NanoparticlesHeterobimetallic Porphyrin ComplexesEnzyme Mimicshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The covalent binding of the porphyrin to immobilized gold nanoparticles resulted in a very useful and simple method to evaluate the absorption spectroscopy of a monolayer of porphyrins and the spectral changes produced by additional ligands. The layer by layer deposition showed no distinguishable porphyrin-related absorption bands whereas the direct simultaneous binding of porphyrins (Cu(II) and Fe(III) tetrapyridylporphine) to immobilized gold nanoparticles showed a significantly higher absorption band at 412 nm corresponding to the Soret band of the porphyrin ring. The ATR-FTIR spectra showed typical absorptions bands of porphyrin core at around 1000 cm−1 and 800 cm−1, which were only observed in the Cu(II) complex of TPyP/AuNPs–APTES/SiO2 suggesting that the CuTPyP molecule is tilted with respect to the substrate surface. The presence of FeTPyP produces changes in the geometry of the adsorbed CuTPyP porphyrins, indicating a flat accommodation of the porphyrinic rings in FeTPyP–CuTPyP composite that could be the reason of the differential tendency to include H2O2 as ligand. The difference UV–visible spectra of the bimetallic structures showed a very sensitive response to H2O2 concentration with a Kapp 3.75 × 103 M−1(R: 0.9865; Std E: 2.6 × 10−2) which was notoriously similar to Km value for peroxidase enzyme (2.5 × 103 M−1).Fil: Hamer, Mariana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carballo, Romina Raquel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rezzano, Irene Noemí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18169Hamer, Mariana; Carballo, Romina Raquel; Rezzano, Irene Noemí; Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles; Elsevier Science; Sensors and Actuators B: Chemical; 160; 1; 12-2011; 1282-12870925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2011.09.063info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400511008653info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:14Zoai:ri.conicet.gov.ar:11336/18169instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:14.731CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles
title Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles
spellingShingle Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles
Hamer, Mariana
Gold Nanoparticles
Heterobimetallic Porphyrin Complexes
Enzyme Mimics
title_short Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles
title_full Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles
title_fullStr Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles
title_full_unstemmed Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles
title_sort Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles
dc.creator.none.fl_str_mv Hamer, Mariana
Carballo, Romina Raquel
Rezzano, Irene Noemí
author Hamer, Mariana
author_facet Hamer, Mariana
Carballo, Romina Raquel
Rezzano, Irene Noemí
author_role author
author2 Carballo, Romina Raquel
Rezzano, Irene Noemí
author2_role author
author
dc.subject.none.fl_str_mv Gold Nanoparticles
Heterobimetallic Porphyrin Complexes
Enzyme Mimics
topic Gold Nanoparticles
Heterobimetallic Porphyrin Complexes
Enzyme Mimics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The covalent binding of the porphyrin to immobilized gold nanoparticles resulted in a very useful and simple method to evaluate the absorption spectroscopy of a monolayer of porphyrins and the spectral changes produced by additional ligands. The layer by layer deposition showed no distinguishable porphyrin-related absorption bands whereas the direct simultaneous binding of porphyrins (Cu(II) and Fe(III) tetrapyridylporphine) to immobilized gold nanoparticles showed a significantly higher absorption band at 412 nm corresponding to the Soret band of the porphyrin ring. The ATR-FTIR spectra showed typical absorptions bands of porphyrin core at around 1000 cm−1 and 800 cm−1, which were only observed in the Cu(II) complex of TPyP/AuNPs–APTES/SiO2 suggesting that the CuTPyP molecule is tilted with respect to the substrate surface. The presence of FeTPyP produces changes in the geometry of the adsorbed CuTPyP porphyrins, indicating a flat accommodation of the porphyrinic rings in FeTPyP–CuTPyP composite that could be the reason of the differential tendency to include H2O2 as ligand. The difference UV–visible spectra of the bimetallic structures showed a very sensitive response to H2O2 concentration with a Kapp 3.75 × 103 M−1(R: 0.9865; Std E: 2.6 × 10−2) which was notoriously similar to Km value for peroxidase enzyme (2.5 × 103 M−1).
Fil: Hamer, Mariana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carballo, Romina Raquel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rezzano, Irene Noemí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The covalent binding of the porphyrin to immobilized gold nanoparticles resulted in a very useful and simple method to evaluate the absorption spectroscopy of a monolayer of porphyrins and the spectral changes produced by additional ligands. The layer by layer deposition showed no distinguishable porphyrin-related absorption bands whereas the direct simultaneous binding of porphyrins (Cu(II) and Fe(III) tetrapyridylporphine) to immobilized gold nanoparticles showed a significantly higher absorption band at 412 nm corresponding to the Soret band of the porphyrin ring. The ATR-FTIR spectra showed typical absorptions bands of porphyrin core at around 1000 cm−1 and 800 cm−1, which were only observed in the Cu(II) complex of TPyP/AuNPs–APTES/SiO2 suggesting that the CuTPyP molecule is tilted with respect to the substrate surface. The presence of FeTPyP produces changes in the geometry of the adsorbed CuTPyP porphyrins, indicating a flat accommodation of the porphyrinic rings in FeTPyP–CuTPyP composite that could be the reason of the differential tendency to include H2O2 as ligand. The difference UV–visible spectra of the bimetallic structures showed a very sensitive response to H2O2 concentration with a Kapp 3.75 × 103 M−1(R: 0.9865; Std E: 2.6 × 10−2) which was notoriously similar to Km value for peroxidase enzyme (2.5 × 103 M−1).
publishDate 2011
dc.date.none.fl_str_mv 2011-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18169
Hamer, Mariana; Carballo, Romina Raquel; Rezzano, Irene Noemí; Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles; Elsevier Science; Sensors and Actuators B: Chemical; 160; 1; 12-2011; 1282-1287
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18169
identifier_str_mv Hamer, Mariana; Carballo, Romina Raquel; Rezzano, Irene Noemí; Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles; Elsevier Science; Sensors and Actuators B: Chemical; 160; 1; 12-2011; 1282-1287
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2011.09.063
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400511008653
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613781087322112
score 13.070432