Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles
- Autores
- Hamer, Mariana; Carballo, Romina Raquel; Rezzano, Irene Noemí
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The covalent binding of the porphyrin to immobilized gold nanoparticles resulted in a very useful and simple method to evaluate the absorption spectroscopy of a monolayer of porphyrins and the spectral changes produced by additional ligands. The layer by layer deposition showed no distinguishable porphyrin-related absorption bands whereas the direct simultaneous binding of porphyrins (Cu(II) and Fe(III) tetrapyridylporphine) to immobilized gold nanoparticles showed a significantly higher absorption band at 412 nm corresponding to the Soret band of the porphyrin ring. The ATR-FTIR spectra showed typical absorptions bands of porphyrin core at around 1000 cm−1 and 800 cm−1, which were only observed in the Cu(II) complex of TPyP/AuNPs–APTES/SiO2 suggesting that the CuTPyP molecule is tilted with respect to the substrate surface. The presence of FeTPyP produces changes in the geometry of the adsorbed CuTPyP porphyrins, indicating a flat accommodation of the porphyrinic rings in FeTPyP–CuTPyP composite that could be the reason of the differential tendency to include H2O2 as ligand. The difference UV–visible spectra of the bimetallic structures showed a very sensitive response to H2O2 concentration with a Kapp 3.75 × 103 M−1(R: 0.9865; Std E: 2.6 × 10−2) which was notoriously similar to Km value for peroxidase enzyme (2.5 × 103 M−1).
Fil: Hamer, Mariana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carballo, Romina Raquel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rezzano, Irene Noemí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Gold Nanoparticles
Heterobimetallic Porphyrin Complexes
Enzyme Mimics - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/18169
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Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticlesHamer, MarianaCarballo, Romina RaquelRezzano, Irene NoemíGold NanoparticlesHeterobimetallic Porphyrin ComplexesEnzyme Mimicshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The covalent binding of the porphyrin to immobilized gold nanoparticles resulted in a very useful and simple method to evaluate the absorption spectroscopy of a monolayer of porphyrins and the spectral changes produced by additional ligands. The layer by layer deposition showed no distinguishable porphyrin-related absorption bands whereas the direct simultaneous binding of porphyrins (Cu(II) and Fe(III) tetrapyridylporphine) to immobilized gold nanoparticles showed a significantly higher absorption band at 412 nm corresponding to the Soret band of the porphyrin ring. The ATR-FTIR spectra showed typical absorptions bands of porphyrin core at around 1000 cm−1 and 800 cm−1, which were only observed in the Cu(II) complex of TPyP/AuNPs–APTES/SiO2 suggesting that the CuTPyP molecule is tilted with respect to the substrate surface. The presence of FeTPyP produces changes in the geometry of the adsorbed CuTPyP porphyrins, indicating a flat accommodation of the porphyrinic rings in FeTPyP–CuTPyP composite that could be the reason of the differential tendency to include H2O2 as ligand. The difference UV–visible spectra of the bimetallic structures showed a very sensitive response to H2O2 concentration with a Kapp 3.75 × 103 M−1(R: 0.9865; Std E: 2.6 × 10−2) which was notoriously similar to Km value for peroxidase enzyme (2.5 × 103 M−1).Fil: Hamer, Mariana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carballo, Romina Raquel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rezzano, Irene Noemí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18169Hamer, Mariana; Carballo, Romina Raquel; Rezzano, Irene Noemí; Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles; Elsevier Science; Sensors and Actuators B: Chemical; 160; 1; 12-2011; 1282-12870925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2011.09.063info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400511008653info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:14Zoai:ri.conicet.gov.ar:11336/18169instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:14.731CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles |
title |
Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles |
spellingShingle |
Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles Hamer, Mariana Gold Nanoparticles Heterobimetallic Porphyrin Complexes Enzyme Mimics |
title_short |
Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles |
title_full |
Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles |
title_fullStr |
Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles |
title_full_unstemmed |
Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles |
title_sort |
Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles |
dc.creator.none.fl_str_mv |
Hamer, Mariana Carballo, Romina Raquel Rezzano, Irene Noemí |
author |
Hamer, Mariana |
author_facet |
Hamer, Mariana Carballo, Romina Raquel Rezzano, Irene Noemí |
author_role |
author |
author2 |
Carballo, Romina Raquel Rezzano, Irene Noemí |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Gold Nanoparticles Heterobimetallic Porphyrin Complexes Enzyme Mimics |
topic |
Gold Nanoparticles Heterobimetallic Porphyrin Complexes Enzyme Mimics |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The covalent binding of the porphyrin to immobilized gold nanoparticles resulted in a very useful and simple method to evaluate the absorption spectroscopy of a monolayer of porphyrins and the spectral changes produced by additional ligands. The layer by layer deposition showed no distinguishable porphyrin-related absorption bands whereas the direct simultaneous binding of porphyrins (Cu(II) and Fe(III) tetrapyridylporphine) to immobilized gold nanoparticles showed a significantly higher absorption band at 412 nm corresponding to the Soret band of the porphyrin ring. The ATR-FTIR spectra showed typical absorptions bands of porphyrin core at around 1000 cm−1 and 800 cm−1, which were only observed in the Cu(II) complex of TPyP/AuNPs–APTES/SiO2 suggesting that the CuTPyP molecule is tilted with respect to the substrate surface. The presence of FeTPyP produces changes in the geometry of the adsorbed CuTPyP porphyrins, indicating a flat accommodation of the porphyrinic rings in FeTPyP–CuTPyP composite that could be the reason of the differential tendency to include H2O2 as ligand. The difference UV–visible spectra of the bimetallic structures showed a very sensitive response to H2O2 concentration with a Kapp 3.75 × 103 M−1(R: 0.9865; Std E: 2.6 × 10−2) which was notoriously similar to Km value for peroxidase enzyme (2.5 × 103 M−1). Fil: Hamer, Mariana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carballo, Romina Raquel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rezzano, Irene Noemí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
The covalent binding of the porphyrin to immobilized gold nanoparticles resulted in a very useful and simple method to evaluate the absorption spectroscopy of a monolayer of porphyrins and the spectral changes produced by additional ligands. The layer by layer deposition showed no distinguishable porphyrin-related absorption bands whereas the direct simultaneous binding of porphyrins (Cu(II) and Fe(III) tetrapyridylporphine) to immobilized gold nanoparticles showed a significantly higher absorption band at 412 nm corresponding to the Soret band of the porphyrin ring. The ATR-FTIR spectra showed typical absorptions bands of porphyrin core at around 1000 cm−1 and 800 cm−1, which were only observed in the Cu(II) complex of TPyP/AuNPs–APTES/SiO2 suggesting that the CuTPyP molecule is tilted with respect to the substrate surface. The presence of FeTPyP produces changes in the geometry of the adsorbed CuTPyP porphyrins, indicating a flat accommodation of the porphyrinic rings in FeTPyP–CuTPyP composite that could be the reason of the differential tendency to include H2O2 as ligand. The difference UV–visible spectra of the bimetallic structures showed a very sensitive response to H2O2 concentration with a Kapp 3.75 × 103 M−1(R: 0.9865; Std E: 2.6 × 10−2) which was notoriously similar to Km value for peroxidase enzyme (2.5 × 103 M−1). |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/18169 Hamer, Mariana; Carballo, Romina Raquel; Rezzano, Irene Noemí; Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles; Elsevier Science; Sensors and Actuators B: Chemical; 160; 1; 12-2011; 1282-1287 0925-4005 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/18169 |
identifier_str_mv |
Hamer, Mariana; Carballo, Romina Raquel; Rezzano, Irene Noemí; Enzyme mimic of heterobimetallic Cu(II) and Fe(III) tetrapyridylporphine modified nanoparticles; Elsevier Science; Sensors and Actuators B: Chemical; 160; 1; 12-2011; 1282-1287 0925-4005 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2011.09.063 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400511008653 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613781087322112 |
score |
13.070432 |