Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria
- Autores
- Martínez Júlvez, Marta; Goñi Rasia, Guillermina Marcela; Pérez Amigot, Daniel; Laplaza, Rubén; Ionescu, Irina Alexandra; Petrocelli, Silvana; Tondo, Maria Laura; Sancho, Javier; Orellano, Elena Graciela; Medina, Milagros
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Ferredoxin-NADP(H) reductases (FNRs) deliver NADPH or low potential one-electron donors to redox-based metabolism in plastids and bacteria. Xanthomonas citri subsp. citri (Xcc) is a Gram-negative bacterium responsible for citrus canker disease that affects commercial citrus crops worldwide. The Xcc fpr gene encodes a bacterial type FNR (XccFPR) that contributes to the bacterial response to oxidative stress conditions, usually found during plant colonization. Therefore, XccFPR is relevant for the pathogen survival and its inhibition might represent a strategy to treat citrus canker. Because of mechanistic and structural differences from plastidic FNRs, XccFPR is also a potential antibacterial target. We have optimized an activity-based high-throughput screening (HTS) assay that identifies XccFPR inhibitors. We selected 43 hits from a chemical library and narrowed them down to the four most promising inhibitors. The antimicrobial effect of these compounds was evaluated on Xcc cultures, finding one with antimicrobial properties. Based on the functional groups of this compound and their geometric arrangement, we identified another three XccFPR inhibitors. Inhibition mechanisms and constants were determined for these four XccFPR inhibitors. Their specificity was also evaluated by studying their effect on the plastidic Anabaena PCC 7119 FNR, finding differences that can become interesting tools to discover Xcc antimicrobials.
Fil: Martínez Júlvez, Marta. Universidad de Zaragoza; España
Fil: Goñi Rasia, Guillermina Marcela. Universidad de Zaragoza; España
Fil: Pérez Amigot, Daniel. Universidad de Zaragoza; España
Fil: Laplaza, Rubén. Universidad de Zaragoza; España. Universidad de Santiago de Compostela; España
Fil: Ionescu, Irina Alexandra. Universidad de Zaragoza; España
Fil: Petrocelli, Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Tondo, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Sancho, Javier. Universidad de Zaragoza; España
Fil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Medina, Milagros. Universidad de Zaragoza; España - Materia
-
ACTIVITY-BASED HIGH-THROUGHPUT SCREENING
ENZYME INHIBITORS
FERREDOXIN-NADP(H) REDUCTASE
XANTHOMONAS CITRI SUBSP. CITRI - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/53227
Ver los metadatos del registro completo
id |
CONICETDig_afcd2491b5b1f35fff9ee5f9f5603537 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/53227 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteriaMartínez Júlvez, MartaGoñi Rasia, Guillermina MarcelaPérez Amigot, DanielLaplaza, RubénIonescu, Irina AlexandraPetrocelli, SilvanaTondo, Maria LauraSancho, JavierOrellano, Elena GracielaMedina, MilagrosACTIVITY-BASED HIGH-THROUGHPUT SCREENINGENZYME INHIBITORSFERREDOXIN-NADP(H) REDUCTASEXANTHOMONAS CITRI SUBSP. CITRIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ferredoxin-NADP(H) reductases (FNRs) deliver NADPH or low potential one-electron donors to redox-based metabolism in plastids and bacteria. Xanthomonas citri subsp. citri (Xcc) is a Gram-negative bacterium responsible for citrus canker disease that affects commercial citrus crops worldwide. The Xcc fpr gene encodes a bacterial type FNR (XccFPR) that contributes to the bacterial response to oxidative stress conditions, usually found during plant colonization. Therefore, XccFPR is relevant for the pathogen survival and its inhibition might represent a strategy to treat citrus canker. Because of mechanistic and structural differences from plastidic FNRs, XccFPR is also a potential antibacterial target. We have optimized an activity-based high-throughput screening (HTS) assay that identifies XccFPR inhibitors. We selected 43 hits from a chemical library and narrowed them down to the four most promising inhibitors. The antimicrobial effect of these compounds was evaluated on Xcc cultures, finding one with antimicrobial properties. Based on the functional groups of this compound and their geometric arrangement, we identified another three XccFPR inhibitors. Inhibition mechanisms and constants were determined for these four XccFPR inhibitors. Their specificity was also evaluated by studying their effect on the plastidic Anabaena PCC 7119 FNR, finding differences that can become interesting tools to discover Xcc antimicrobials.Fil: Martínez Júlvez, Marta. Universidad de Zaragoza; EspañaFil: Goñi Rasia, Guillermina Marcela. Universidad de Zaragoza; EspañaFil: Pérez Amigot, Daniel. Universidad de Zaragoza; EspañaFil: Laplaza, Rubén. Universidad de Zaragoza; España. Universidad de Santiago de Compostela; EspañaFil: Ionescu, Irina Alexandra. Universidad de Zaragoza; EspañaFil: Petrocelli, Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Tondo, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Sancho, Javier. Universidad de Zaragoza; EspañaFil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Medina, Milagros. Universidad de Zaragoza; EspañaMolecular Diversity Preservation International2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53227Martínez Júlvez, Marta; Goñi Rasia, Guillermina Marcela; Pérez Amigot, Daniel; Laplaza, Rubén; Ionescu, Irina Alexandra; et al.; Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria; Molecular Diversity Preservation International; Molecules; 23; 1; 12-2017; 1-151420-3049CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/molecules23010029info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1420-3049/23/1/29info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:51Zoai:ri.conicet.gov.ar:11336/53227instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:51.331CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria |
title |
Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria |
spellingShingle |
Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria Martínez Júlvez, Marta ACTIVITY-BASED HIGH-THROUGHPUT SCREENING ENZYME INHIBITORS FERREDOXIN-NADP(H) REDUCTASE XANTHOMONAS CITRI SUBSP. CITRI |
title_short |
Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria |
title_full |
Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria |
title_fullStr |
Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria |
title_full_unstemmed |
Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria |
title_sort |
Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria |
dc.creator.none.fl_str_mv |
Martínez Júlvez, Marta Goñi Rasia, Guillermina Marcela Pérez Amigot, Daniel Laplaza, Rubén Ionescu, Irina Alexandra Petrocelli, Silvana Tondo, Maria Laura Sancho, Javier Orellano, Elena Graciela Medina, Milagros |
author |
Martínez Júlvez, Marta |
author_facet |
Martínez Júlvez, Marta Goñi Rasia, Guillermina Marcela Pérez Amigot, Daniel Laplaza, Rubén Ionescu, Irina Alexandra Petrocelli, Silvana Tondo, Maria Laura Sancho, Javier Orellano, Elena Graciela Medina, Milagros |
author_role |
author |
author2 |
Goñi Rasia, Guillermina Marcela Pérez Amigot, Daniel Laplaza, Rubén Ionescu, Irina Alexandra Petrocelli, Silvana Tondo, Maria Laura Sancho, Javier Orellano, Elena Graciela Medina, Milagros |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
ACTIVITY-BASED HIGH-THROUGHPUT SCREENING ENZYME INHIBITORS FERREDOXIN-NADP(H) REDUCTASE XANTHOMONAS CITRI SUBSP. CITRI |
topic |
ACTIVITY-BASED HIGH-THROUGHPUT SCREENING ENZYME INHIBITORS FERREDOXIN-NADP(H) REDUCTASE XANTHOMONAS CITRI SUBSP. CITRI |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Ferredoxin-NADP(H) reductases (FNRs) deliver NADPH or low potential one-electron donors to redox-based metabolism in plastids and bacteria. Xanthomonas citri subsp. citri (Xcc) is a Gram-negative bacterium responsible for citrus canker disease that affects commercial citrus crops worldwide. The Xcc fpr gene encodes a bacterial type FNR (XccFPR) that contributes to the bacterial response to oxidative stress conditions, usually found during plant colonization. Therefore, XccFPR is relevant for the pathogen survival and its inhibition might represent a strategy to treat citrus canker. Because of mechanistic and structural differences from plastidic FNRs, XccFPR is also a potential antibacterial target. We have optimized an activity-based high-throughput screening (HTS) assay that identifies XccFPR inhibitors. We selected 43 hits from a chemical library and narrowed them down to the four most promising inhibitors. The antimicrobial effect of these compounds was evaluated on Xcc cultures, finding one with antimicrobial properties. Based on the functional groups of this compound and their geometric arrangement, we identified another three XccFPR inhibitors. Inhibition mechanisms and constants were determined for these four XccFPR inhibitors. Their specificity was also evaluated by studying their effect on the plastidic Anabaena PCC 7119 FNR, finding differences that can become interesting tools to discover Xcc antimicrobials. Fil: Martínez Júlvez, Marta. Universidad de Zaragoza; España Fil: Goñi Rasia, Guillermina Marcela. Universidad de Zaragoza; España Fil: Pérez Amigot, Daniel. Universidad de Zaragoza; España Fil: Laplaza, Rubén. Universidad de Zaragoza; España. Universidad de Santiago de Compostela; España Fil: Ionescu, Irina Alexandra. Universidad de Zaragoza; España Fil: Petrocelli, Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Tondo, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Sancho, Javier. Universidad de Zaragoza; España Fil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Medina, Milagros. Universidad de Zaragoza; España |
description |
Ferredoxin-NADP(H) reductases (FNRs) deliver NADPH or low potential one-electron donors to redox-based metabolism in plastids and bacteria. Xanthomonas citri subsp. citri (Xcc) is a Gram-negative bacterium responsible for citrus canker disease that affects commercial citrus crops worldwide. The Xcc fpr gene encodes a bacterial type FNR (XccFPR) that contributes to the bacterial response to oxidative stress conditions, usually found during plant colonization. Therefore, XccFPR is relevant for the pathogen survival and its inhibition might represent a strategy to treat citrus canker. Because of mechanistic and structural differences from plastidic FNRs, XccFPR is also a potential antibacterial target. We have optimized an activity-based high-throughput screening (HTS) assay that identifies XccFPR inhibitors. We selected 43 hits from a chemical library and narrowed them down to the four most promising inhibitors. The antimicrobial effect of these compounds was evaluated on Xcc cultures, finding one with antimicrobial properties. Based on the functional groups of this compound and their geometric arrangement, we identified another three XccFPR inhibitors. Inhibition mechanisms and constants were determined for these four XccFPR inhibitors. Their specificity was also evaluated by studying their effect on the plastidic Anabaena PCC 7119 FNR, finding differences that can become interesting tools to discover Xcc antimicrobials. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/53227 Martínez Júlvez, Marta; Goñi Rasia, Guillermina Marcela; Pérez Amigot, Daniel; Laplaza, Rubén; Ionescu, Irina Alexandra; et al.; Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria; Molecular Diversity Preservation International; Molecules; 23; 1; 12-2017; 1-15 1420-3049 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/53227 |
identifier_str_mv |
Martínez Júlvez, Marta; Goñi Rasia, Guillermina Marcela; Pérez Amigot, Daniel; Laplaza, Rubén; Ionescu, Irina Alexandra; et al.; Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria; Molecular Diversity Preservation International; Molecules; 23; 1; 12-2017; 1-15 1420-3049 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules23010029 info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1420-3049/23/1/29 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842269774305820672 |
score |
13.13397 |