Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content

Autores
Cappa, Andrea; de Paola, Maria Matilde; Wetten, Paula; de Blas, Gerardo Andrés; Michaut, Marcela Alejandra
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oocyte in vitro maturation does not entirely support all the nuclear and cytoplasmic changes that occur physiologically and it is poorly understood whether in vitro maturation affects the competence of cortical granules to secret their content during cortical reaction. Here, we characterize cortical granule exocytosis (CGE) in live mouse oocytes activated by strontium chloride using the fluorescent lectin FITC-LCA. We compared the kinetic of CGE between ovulated (in vivo matured, IVO) and in vitro matured (IVM) mouse oocytes. Results show that: 1) IVM oocytes have a severely reduced response to strontium chloride; 2) the low response was confirmed by quantification of remnant cortical granules in permeabilized cells and by a novel method to quantify the exudate in non permeabilized cells; 3) the kinetic of CGE in IVO oocytes was rapid and synchronous; 4) the kinetic of CGE in IVM oocytes was delayed and asynchronous; 5) cortical granules in IVM oocytes show an irregular limit with the cortical granule free domain. We propose the analysis of CGE in live oocytes as a biological test to evaluate the competence of IVM mouse oocytes.
Fil: Cappa, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: de Paola, Maria Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Wetten, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: de Blas, Gerardo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Michaut, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
CORTICAL GRANULE EXOCYTOSIS
REAL TIME
IN VITRO MATURATION
MOUSE OOCYTE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92629
Acceder
id CONICETDig_afb07567c43ba33ab3f49005e207ac15
oai_identifier_str oai:ri.conicet.gov.ar:11336/92629
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their contentCappa, Andreade Paola, Maria MatildeWetten, Paulade Blas, Gerardo AndrésMichaut, Marcela AlejandraCORTICAL GRANULE EXOCYTOSISREAL TIMEIN VITRO MATURATIONMOUSE OOCYTEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Oocyte in vitro maturation does not entirely support all the nuclear and cytoplasmic changes that occur physiologically and it is poorly understood whether in vitro maturation affects the competence of cortical granules to secret their content during cortical reaction. Here, we characterize cortical granule exocytosis (CGE) in live mouse oocytes activated by strontium chloride using the fluorescent lectin FITC-LCA. We compared the kinetic of CGE between ovulated (in vivo matured, IVO) and in vitro matured (IVM) mouse oocytes. Results show that: 1) IVM oocytes have a severely reduced response to strontium chloride; 2) the low response was confirmed by quantification of remnant cortical granules in permeabilized cells and by a novel method to quantify the exudate in non permeabilized cells; 3) the kinetic of CGE in IVO oocytes was rapid and synchronous; 4) the kinetic of CGE in IVM oocytes was delayed and asynchronous; 5) cortical granules in IVM oocytes show an irregular limit with the cortical granule free domain. We propose the analysis of CGE in live oocytes as a biological test to evaluate the competence of IVM mouse oocytes.Fil: Cappa, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: de Paola, Maria Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Wetten, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: de Blas, Gerardo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Michaut, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaCompany of Biologists2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92629Cappa, Andrea; de Paola, Maria Matilde; Wetten, Paula; de Blas, Gerardo Andrés; Michaut, Marcela Alejandra; Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content; Company of Biologists; Biology Open; 10-2018; 1-282046-6390CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1242/bio.031872info:eu-repo/semantics/altIdentifier/url/https://bio.biologists.org/content/7/12/bio031872info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:17:31Zoai:ri.conicet.gov.ar:11336/92629instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:17:31.836CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content
title Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content
spellingShingle Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content
Cappa, Andrea
CORTICAL GRANULE EXOCYTOSIS
REAL TIME
IN VITRO MATURATION
MOUSE OOCYTE
title_short Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content
title_full Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content
title_fullStr Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content
title_full_unstemmed Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content
title_sort Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content
dc.creator.none.fl_str_mv Cappa, Andrea
de Paola, Maria Matilde
Wetten, Paula
de Blas, Gerardo Andrés
Michaut, Marcela Alejandra
author Cappa, Andrea
author_facet Cappa, Andrea
de Paola, Maria Matilde
Wetten, Paula
de Blas, Gerardo Andrés
Michaut, Marcela Alejandra
author_role author
author2 de Paola, Maria Matilde
Wetten, Paula
de Blas, Gerardo Andrés
Michaut, Marcela Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv CORTICAL GRANULE EXOCYTOSIS
REAL TIME
IN VITRO MATURATION
MOUSE OOCYTE
topic CORTICAL GRANULE EXOCYTOSIS
REAL TIME
IN VITRO MATURATION
MOUSE OOCYTE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Oocyte in vitro maturation does not entirely support all the nuclear and cytoplasmic changes that occur physiologically and it is poorly understood whether in vitro maturation affects the competence of cortical granules to secret their content during cortical reaction. Here, we characterize cortical granule exocytosis (CGE) in live mouse oocytes activated by strontium chloride using the fluorescent lectin FITC-LCA. We compared the kinetic of CGE between ovulated (in vivo matured, IVO) and in vitro matured (IVM) mouse oocytes. Results show that: 1) IVM oocytes have a severely reduced response to strontium chloride; 2) the low response was confirmed by quantification of remnant cortical granules in permeabilized cells and by a novel method to quantify the exudate in non permeabilized cells; 3) the kinetic of CGE in IVO oocytes was rapid and synchronous; 4) the kinetic of CGE in IVM oocytes was delayed and asynchronous; 5) cortical granules in IVM oocytes show an irregular limit with the cortical granule free domain. We propose the analysis of CGE in live oocytes as a biological test to evaluate the competence of IVM mouse oocytes.
Fil: Cappa, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: de Paola, Maria Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Wetten, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: de Blas, Gerardo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Michaut, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description Oocyte in vitro maturation does not entirely support all the nuclear and cytoplasmic changes that occur physiologically and it is poorly understood whether in vitro maturation affects the competence of cortical granules to secret their content during cortical reaction. Here, we characterize cortical granule exocytosis (CGE) in live mouse oocytes activated by strontium chloride using the fluorescent lectin FITC-LCA. We compared the kinetic of CGE between ovulated (in vivo matured, IVO) and in vitro matured (IVM) mouse oocytes. Results show that: 1) IVM oocytes have a severely reduced response to strontium chloride; 2) the low response was confirmed by quantification of remnant cortical granules in permeabilized cells and by a novel method to quantify the exudate in non permeabilized cells; 3) the kinetic of CGE in IVO oocytes was rapid and synchronous; 4) the kinetic of CGE in IVM oocytes was delayed and asynchronous; 5) cortical granules in IVM oocytes show an irregular limit with the cortical granule free domain. We propose the analysis of CGE in live oocytes as a biological test to evaluate the competence of IVM mouse oocytes.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92629
Cappa, Andrea; de Paola, Maria Matilde; Wetten, Paula; de Blas, Gerardo Andrés; Michaut, Marcela Alejandra; Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content; Company of Biologists; Biology Open; 10-2018; 1-28
2046-6390
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92629
identifier_str_mv Cappa, Andrea; de Paola, Maria Matilde; Wetten, Paula; de Blas, Gerardo Andrés; Michaut, Marcela Alejandra; Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content; Company of Biologists; Biology Open; 10-2018; 1-28
2046-6390
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1242/bio.031872
info:eu-repo/semantics/altIdentifier/url/https://bio.biologists.org/content/7/12/bio031872
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980955981086720
score 12.993085

Publicaciones similares