Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs

Autores
Gómez Elías, Matías Daniel; Fissore, Rafael A.; Cuasnicu, Patricia Sara; Cohen, Debora Juana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Compensatory endocytosis (CE) is one of the primary mechanisms through whichcells maintain their surface area after exocytosis. Considering that in eggs massiveexocytosis of cortical granules (CG) takes place after fertilization, the aim of this was to evaluate the occurrence of CE following cortical exocytosis in mouseeggs. For this purpose, we developed a pulse‐chase assay to detect CG membraneinternalization. Results showed internalized labeling in SrCl2‐activated and fertilized eggs when chasing at 37°C, but not at a nonpermissive temperature (4°C). The use of kinase and calcineurin inhibitors led us to conclude that this internal labeling corresponded to CE. Further experiments showed that CE in mouse eggs isdependent on actin dynamics and dynamin activity, and could be associated with atransient exposure of phosphatidylserine. Finally, CE was impaired in A23187ionophore‐activated eggs, highlighting once again the mechanistic differencesbetween the activation methods. Altogether, these results demonstrate for the firsttime that egg activation triggers CE in mouse eggs after exocytosis of CG, probably as a plasma membrane homeostasis mechanism.
Fil: Gómez Elías, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Fissore, Rafael A.. University of Massachussets; Estados Unidos
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
COMPENSATORY ENDOCYTOSIS
CORTICAL GRANULES
EGG ACTIVATION
FERTILIZATION
PHOSPHATIDYLSERINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/106316
Acceder
id CONICETDig_d4796627ed27eb0b30bc993c4a17c997
oai_identifier_str oai:ri.conicet.gov.ar:11336/106316
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggsGómez Elías, Matías DanielFissore, Rafael A.Cuasnicu, Patricia SaraCohen, Debora JuanaCOMPENSATORY ENDOCYTOSISCORTICAL GRANULESEGG ACTIVATIONFERTILIZATIONPHOSPHATIDYLSERINEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Compensatory endocytosis (CE) is one of the primary mechanisms through whichcells maintain their surface area after exocytosis. Considering that in eggs massiveexocytosis of cortical granules (CG) takes place after fertilization, the aim of this was to evaluate the occurrence of CE following cortical exocytosis in mouseeggs. For this purpose, we developed a pulse‐chase assay to detect CG membraneinternalization. Results showed internalized labeling in SrCl2‐activated and fertilized eggs when chasing at 37°C, but not at a nonpermissive temperature (4°C). The use of kinase and calcineurin inhibitors led us to conclude that this internal labeling corresponded to CE. Further experiments showed that CE in mouse eggs isdependent on actin dynamics and dynamin activity, and could be associated with atransient exposure of phosphatidylserine. Finally, CE was impaired in A23187ionophore‐activated eggs, highlighting once again the mechanistic differencesbetween the activation methods. Altogether, these results demonstrate for the firsttime that egg activation triggers CE in mouse eggs after exocytosis of CG, probably as a plasma membrane homeostasis mechanism.Fil: Gómez Elías, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Fissore, Rafael A.. University of Massachussets; Estados UnidosFil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaWiley-liss, Div John Wiley & Sons Inc2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/106316Gómez Elías, Matías Daniel; Fissore, Rafael A.; Cuasnicu, Patricia Sara; Cohen, Debora Juana; Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Physiology; 235; 5; 10-2019; 4351-43600021-9541CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29311info:eu-repo/semantics/altIdentifier/doi/10.1002/jcp.29311info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:13Zoai:ri.conicet.gov.ar:11336/106316instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:13.844CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs
title Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs
spellingShingle Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs
Gómez Elías, Matías Daniel
COMPENSATORY ENDOCYTOSIS
CORTICAL GRANULES
EGG ACTIVATION
FERTILIZATION
PHOSPHATIDYLSERINE
title_short Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs
title_full Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs
title_fullStr Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs
title_full_unstemmed Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs
title_sort Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs
dc.creator.none.fl_str_mv Gómez Elías, Matías Daniel
Fissore, Rafael A.
Cuasnicu, Patricia Sara
Cohen, Debora Juana
author Gómez Elías, Matías Daniel
author_facet Gómez Elías, Matías Daniel
Fissore, Rafael A.
Cuasnicu, Patricia Sara
Cohen, Debora Juana
author_role author
author2 Fissore, Rafael A.
Cuasnicu, Patricia Sara
Cohen, Debora Juana
author2_role author
author
author
dc.subject.none.fl_str_mv COMPENSATORY ENDOCYTOSIS
CORTICAL GRANULES
EGG ACTIVATION
FERTILIZATION
PHOSPHATIDYLSERINE
topic COMPENSATORY ENDOCYTOSIS
CORTICAL GRANULES
EGG ACTIVATION
FERTILIZATION
PHOSPHATIDYLSERINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Compensatory endocytosis (CE) is one of the primary mechanisms through whichcells maintain their surface area after exocytosis. Considering that in eggs massiveexocytosis of cortical granules (CG) takes place after fertilization, the aim of this was to evaluate the occurrence of CE following cortical exocytosis in mouseeggs. For this purpose, we developed a pulse‐chase assay to detect CG membraneinternalization. Results showed internalized labeling in SrCl2‐activated and fertilized eggs when chasing at 37°C, but not at a nonpermissive temperature (4°C). The use of kinase and calcineurin inhibitors led us to conclude that this internal labeling corresponded to CE. Further experiments showed that CE in mouse eggs isdependent on actin dynamics and dynamin activity, and could be associated with atransient exposure of phosphatidylserine. Finally, CE was impaired in A23187ionophore‐activated eggs, highlighting once again the mechanistic differencesbetween the activation methods. Altogether, these results demonstrate for the firsttime that egg activation triggers CE in mouse eggs after exocytosis of CG, probably as a plasma membrane homeostasis mechanism.
Fil: Gómez Elías, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Fissore, Rafael A.. University of Massachussets; Estados Unidos
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Compensatory endocytosis (CE) is one of the primary mechanisms through whichcells maintain their surface area after exocytosis. Considering that in eggs massiveexocytosis of cortical granules (CG) takes place after fertilization, the aim of this was to evaluate the occurrence of CE following cortical exocytosis in mouseeggs. For this purpose, we developed a pulse‐chase assay to detect CG membraneinternalization. Results showed internalized labeling in SrCl2‐activated and fertilized eggs when chasing at 37°C, but not at a nonpermissive temperature (4°C). The use of kinase and calcineurin inhibitors led us to conclude that this internal labeling corresponded to CE. Further experiments showed that CE in mouse eggs isdependent on actin dynamics and dynamin activity, and could be associated with atransient exposure of phosphatidylserine. Finally, CE was impaired in A23187ionophore‐activated eggs, highlighting once again the mechanistic differencesbetween the activation methods. Altogether, these results demonstrate for the firsttime that egg activation triggers CE in mouse eggs after exocytosis of CG, probably as a plasma membrane homeostasis mechanism.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/106316
Gómez Elías, Matías Daniel; Fissore, Rafael A.; Cuasnicu, Patricia Sara; Cohen, Debora Juana; Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Physiology; 235; 5; 10-2019; 4351-4360
0021-9541
CONICET Digital
CONICET
url http://hdl.handle.net/11336/106316
identifier_str_mv Gómez Elías, Matías Daniel; Fissore, Rafael A.; Cuasnicu, Patricia Sara; Cohen, Debora Juana; Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Physiology; 235; 5; 10-2019; 4351-4360
0021-9541
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29311
info:eu-repo/semantics/altIdentifier/doi/10.1002/jcp.29311
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613846069673984
score 13.070432

Publicaciones similares