Variability in gut mucosal secretory IgA in mice along a working day

Autores
Burns, Patricia Graciela; Oddi, Sofia Lorena; Forzani, Liliana Maria; Tabacman, Eduardo; Reinheimer, Jorge Alberto; Vinderola, Celso Gabriel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To assess the variability of secretory immunoglobulin A (S-IgA) in the lumen and feces of mice along a working day. Results: Mice were maintained under a 12 h light-dark cycle, light period starting at 8 AM. S-IgA was determined in feces and intestinal content (after one or three washes) at three points along the day: at the beginning, in the middle and at the end of the light period (ELP). Significant reduction in the content of S-IgA in the small intestine fluid and in feces was observed at the end of the light cycle, which coincides with the end of a regular working day (8 PM) in any given animal facility. It was also observed that three washes of the small intestine were more effective than one flush to recover a significant higher amount of S-IgA, with the smallest coefficient of variation observed by the ELP. A smaller CV would imply a reduced number of animals needed to achieve the same meaningful results. The results may be useful when designing animal trials for the selection of probiotic candidates based on their capacity of activating S-IgA, since it would imply a more rational use of experimental animals.
Fil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Oddi, Sofia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Forzani, Liliana Maria. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina
Fil: Tabacman, Eduardo. Somalogic Inc.; Estados Unidos
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Materia
CIRCADIAN RHYTHM
IGA
INTESTINE
MICE
VARIABILITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84872
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/84872
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Variability in gut mucosal secretory IgA in mice along a working dayBurns, Patricia GracielaOddi, Sofia LorenaForzani, Liliana MariaTabacman, EduardoReinheimer, Jorge AlbertoVinderola, Celso GabrielCIRCADIAN RHYTHMIGAINTESTINEMICEVARIABILITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: To assess the variability of secretory immunoglobulin A (S-IgA) in the lumen and feces of mice along a working day. Results: Mice were maintained under a 12 h light-dark cycle, light period starting at 8 AM. S-IgA was determined in feces and intestinal content (after one or three washes) at three points along the day: at the beginning, in the middle and at the end of the light period (ELP). Significant reduction in the content of S-IgA in the small intestine fluid and in feces was observed at the end of the light cycle, which coincides with the end of a regular working day (8 PM) in any given animal facility. It was also observed that three washes of the small intestine were more effective than one flush to recover a significant higher amount of S-IgA, with the smallest coefficient of variation observed by the ELP. A smaller CV would imply a reduced number of animals needed to achieve the same meaningful results. The results may be useful when designing animal trials for the selection of probiotic candidates based on their capacity of activating S-IgA, since it would imply a more rational use of experimental animals.Fil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Oddi, Sofia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Forzani, Liliana Maria. Universidad Nacional del Litoral. Facultad de Ingeniería Química; ArgentinaFil: Tabacman, Eduardo. Somalogic Inc.; Estados UnidosFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaBioMed Central Ltd.2018-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84872Burns, Patricia Graciela; Oddi, Sofia Lorena; Forzani, Liliana Maria; Tabacman, Eduardo; Reinheimer, Jorge Alberto; et al.; Variability in gut mucosal secretory IgA in mice along a working day; BioMed Central Ltd.; BMC Research Notes; 11; 1; 2-2018; 1-51756-0500CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3213-0info:eu-repo/semantics/altIdentifier/doi/10.1186/s13104-018-3213-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:36:19Zoai:ri.conicet.gov.ar:11336/84872instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:36:19.449CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Variability in gut mucosal secretory IgA in mice along a working day
title Variability in gut mucosal secretory IgA in mice along a working day
spellingShingle Variability in gut mucosal secretory IgA in mice along a working day
Burns, Patricia Graciela
CIRCADIAN RHYTHM
IGA
INTESTINE
MICE
VARIABILITY
title_short Variability in gut mucosal secretory IgA in mice along a working day
title_full Variability in gut mucosal secretory IgA in mice along a working day
title_fullStr Variability in gut mucosal secretory IgA in mice along a working day
title_full_unstemmed Variability in gut mucosal secretory IgA in mice along a working day
title_sort Variability in gut mucosal secretory IgA in mice along a working day
dc.creator.none.fl_str_mv Burns, Patricia Graciela
Oddi, Sofia Lorena
Forzani, Liliana Maria
Tabacman, Eduardo
Reinheimer, Jorge Alberto
Vinderola, Celso Gabriel
author Burns, Patricia Graciela
author_facet Burns, Patricia Graciela
Oddi, Sofia Lorena
Forzani, Liliana Maria
Tabacman, Eduardo
Reinheimer, Jorge Alberto
Vinderola, Celso Gabriel
author_role author
author2 Oddi, Sofia Lorena
Forzani, Liliana Maria
Tabacman, Eduardo
Reinheimer, Jorge Alberto
Vinderola, Celso Gabriel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CIRCADIAN RHYTHM
IGA
INTESTINE
MICE
VARIABILITY
topic CIRCADIAN RHYTHM
IGA
INTESTINE
MICE
VARIABILITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: To assess the variability of secretory immunoglobulin A (S-IgA) in the lumen and feces of mice along a working day. Results: Mice were maintained under a 12 h light-dark cycle, light period starting at 8 AM. S-IgA was determined in feces and intestinal content (after one or three washes) at three points along the day: at the beginning, in the middle and at the end of the light period (ELP). Significant reduction in the content of S-IgA in the small intestine fluid and in feces was observed at the end of the light cycle, which coincides with the end of a regular working day (8 PM) in any given animal facility. It was also observed that three washes of the small intestine were more effective than one flush to recover a significant higher amount of S-IgA, with the smallest coefficient of variation observed by the ELP. A smaller CV would imply a reduced number of animals needed to achieve the same meaningful results. The results may be useful when designing animal trials for the selection of probiotic candidates based on their capacity of activating S-IgA, since it would imply a more rational use of experimental animals.
Fil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Oddi, Sofia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Forzani, Liliana Maria. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina
Fil: Tabacman, Eduardo. Somalogic Inc.; Estados Unidos
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
description Objective: To assess the variability of secretory immunoglobulin A (S-IgA) in the lumen and feces of mice along a working day. Results: Mice were maintained under a 12 h light-dark cycle, light period starting at 8 AM. S-IgA was determined in feces and intestinal content (after one or three washes) at three points along the day: at the beginning, in the middle and at the end of the light period (ELP). Significant reduction in the content of S-IgA in the small intestine fluid and in feces was observed at the end of the light cycle, which coincides with the end of a regular working day (8 PM) in any given animal facility. It was also observed that three washes of the small intestine were more effective than one flush to recover a significant higher amount of S-IgA, with the smallest coefficient of variation observed by the ELP. A smaller CV would imply a reduced number of animals needed to achieve the same meaningful results. The results may be useful when designing animal trials for the selection of probiotic candidates based on their capacity of activating S-IgA, since it would imply a more rational use of experimental animals.
publishDate 2018
dc.date.none.fl_str_mv 2018-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84872
Burns, Patricia Graciela; Oddi, Sofia Lorena; Forzani, Liliana Maria; Tabacman, Eduardo; Reinheimer, Jorge Alberto; et al.; Variability in gut mucosal secretory IgA in mice along a working day; BioMed Central Ltd.; BMC Research Notes; 11; 1; 2-2018; 1-5
1756-0500
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84872
identifier_str_mv Burns, Patricia Graciela; Oddi, Sofia Lorena; Forzani, Liliana Maria; Tabacman, Eduardo; Reinheimer, Jorge Alberto; et al.; Variability in gut mucosal secretory IgA in mice along a working day; BioMed Central Ltd.; BMC Research Notes; 11; 1; 2-2018; 1-5
1756-0500
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3213-0
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13104-018-3213-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central Ltd.
publisher.none.fl_str_mv BioMed Central Ltd.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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