IgA Production Alter Administration of a Probiotic

Autores
Gigola, G.; Gandini, Norberto Ariel; Curino, Alejandro Carlos; Fermento, María Eugenia; Ullua, Natalia Paola; Maturi, Horacio Vicente; Perez, Jose Emanuel; Perdigon, Gabriela del Valle
Año de publicación
2007
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Probiotics are live microorganisms that exert health beneficial actions to the host after their oral administration if they are given in suitable amounts. They have a stimulatory effect on humoral immunity by increasing the amount of available local and circulating IgA. However, it is possible that continuos administration might not be the optimal way to administer them. Our objective was to compare two doses of probiotics and study their effect on local immunity in rat intestine. For this purpose we used 27 rats that were divided in three groups: Control group (3 animals), Group A (15 animals ) and group B (9 animals ). Group A was given 1 ml of BIOFLORA (SIDUS Lab) and Group B 0,2 ml. Animals were sacrificed at 3,5,8,10 and 15 weeks of treatment and intestine was resected and processed by freezing technique. Immunofluorescence was performed in order to study the amount of IgA+ cells. Results: BIOFLORA stimulated the production of IgA secretory cells in groupAwith a peak at 3 days whereas in group B it did not exert significant effects with respect to control animals. The amount of IgA+ cells returned to basal values after 8 days of treatment. The results suggest that the increase in IgAsecretory cells is dependent on the dose of probiotic and the period of treatment and that it is possible that a cyclic treatment would be a better way of administering the probiotic
Fil: Gigola, G.. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Ullua, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Maturi, Horacio Vicente. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Perez, Jose Emanuel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
XLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
IGA
PROBIOTICOS
MICROORGANISMS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243736
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling IgA Production Alter Administration of a ProbioticGigola, G.Gandini, Norberto ArielCurino, Alejandro CarlosFermento, María EugeniaUllua, Natalia PaolaMaturi, Horacio VicentePerez, Jose EmanuelPerdigon, Gabriela del ValleIGAPROBIOTICOSMICROORGANISMShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Probiotics are live microorganisms that exert health beneficial actions to the host after their oral administration if they are given in suitable amounts. They have a stimulatory effect on humoral immunity by increasing the amount of available local and circulating IgA. However, it is possible that continuos administration might not be the optimal way to administer them. Our objective was to compare two doses of probiotics and study their effect on local immunity in rat intestine. For this purpose we used 27 rats that were divided in three groups: Control group (3 animals), Group A (15 animals ) and group B (9 animals ). Group A was given 1 ml of BIOFLORA (SIDUS Lab) and Group B 0,2 ml. Animals were sacrificed at 3,5,8,10 and 15 weeks of treatment and intestine was resected and processed by freezing technique. Immunofluorescence was performed in order to study the amount of IgA+ cells. Results: BIOFLORA stimulated the production of IgA secretory cells in groupAwith a peak at 3 days whereas in group B it did not exert significant effects with respect to control animals. The amount of IgA+ cells returned to basal values after 8 days of treatment. The results suggest that the increase in IgAsecretory cells is dependent on the dose of probiotic and the period of treatment and that it is possible that a cyclic treatment would be a better way of administering the probioticFil: Gigola, G.. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Ullua, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Maturi, Horacio Vicente. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Perez, Jose Emanuel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaXLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularMar del PlataArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243736IgA Production Alter Administration of a Probiotic; XLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Mar del Plata; Argentina; 2007; 84-840327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://newsite.saib.org.ar/publicaciones/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:10:09Zoai:ri.conicet.gov.ar:11336/243736instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:10:10.004CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv IgA Production Alter Administration of a Probiotic
title IgA Production Alter Administration of a Probiotic
spellingShingle IgA Production Alter Administration of a Probiotic
Gigola, G.
IGA
PROBIOTICOS
MICROORGANISMS
title_short IgA Production Alter Administration of a Probiotic
title_full IgA Production Alter Administration of a Probiotic
title_fullStr IgA Production Alter Administration of a Probiotic
title_full_unstemmed IgA Production Alter Administration of a Probiotic
title_sort IgA Production Alter Administration of a Probiotic
dc.creator.none.fl_str_mv Gigola, G.
Gandini, Norberto Ariel
Curino, Alejandro Carlos
Fermento, María Eugenia
Ullua, Natalia Paola
Maturi, Horacio Vicente
Perez, Jose Emanuel
Perdigon, Gabriela del Valle
author Gigola, G.
author_facet Gigola, G.
Gandini, Norberto Ariel
Curino, Alejandro Carlos
Fermento, María Eugenia
Ullua, Natalia Paola
Maturi, Horacio Vicente
Perez, Jose Emanuel
Perdigon, Gabriela del Valle
author_role author
author2 Gandini, Norberto Ariel
Curino, Alejandro Carlos
Fermento, María Eugenia
Ullua, Natalia Paola
Maturi, Horacio Vicente
Perez, Jose Emanuel
Perdigon, Gabriela del Valle
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv IGA
PROBIOTICOS
MICROORGANISMS
topic IGA
PROBIOTICOS
MICROORGANISMS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Probiotics are live microorganisms that exert health beneficial actions to the host after their oral administration if they are given in suitable amounts. They have a stimulatory effect on humoral immunity by increasing the amount of available local and circulating IgA. However, it is possible that continuos administration might not be the optimal way to administer them. Our objective was to compare two doses of probiotics and study their effect on local immunity in rat intestine. For this purpose we used 27 rats that were divided in three groups: Control group (3 animals), Group A (15 animals ) and group B (9 animals ). Group A was given 1 ml of BIOFLORA (SIDUS Lab) and Group B 0,2 ml. Animals were sacrificed at 3,5,8,10 and 15 weeks of treatment and intestine was resected and processed by freezing technique. Immunofluorescence was performed in order to study the amount of IgA+ cells. Results: BIOFLORA stimulated the production of IgA secretory cells in groupAwith a peak at 3 days whereas in group B it did not exert significant effects with respect to control animals. The amount of IgA+ cells returned to basal values after 8 days of treatment. The results suggest that the increase in IgAsecretory cells is dependent on the dose of probiotic and the period of treatment and that it is possible that a cyclic treatment would be a better way of administering the probiotic
Fil: Gigola, G.. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Ullua, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Maturi, Horacio Vicente. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Perez, Jose Emanuel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
XLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Probiotics are live microorganisms that exert health beneficial actions to the host after their oral administration if they are given in suitable amounts. They have a stimulatory effect on humoral immunity by increasing the amount of available local and circulating IgA. However, it is possible that continuos administration might not be the optimal way to administer them. Our objective was to compare two doses of probiotics and study their effect on local immunity in rat intestine. For this purpose we used 27 rats that were divided in three groups: Control group (3 animals), Group A (15 animals ) and group B (9 animals ). Group A was given 1 ml of BIOFLORA (SIDUS Lab) and Group B 0,2 ml. Animals were sacrificed at 3,5,8,10 and 15 weeks of treatment and intestine was resected and processed by freezing technique. Immunofluorescence was performed in order to study the amount of IgA+ cells. Results: BIOFLORA stimulated the production of IgA secretory cells in groupAwith a peak at 3 days whereas in group B it did not exert significant effects with respect to control animals. The amount of IgA+ cells returned to basal values after 8 days of treatment. The results suggest that the increase in IgAsecretory cells is dependent on the dose of probiotic and the period of treatment and that it is possible that a cyclic treatment would be a better way of administering the probiotic
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243736
IgA Production Alter Administration of a Probiotic; XLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Mar del Plata; Argentina; 2007; 84-84
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243736
identifier_str_mv IgA Production Alter Administration of a Probiotic; XLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Mar del Plata; Argentina; 2007; 84-84
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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