Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix

Autores
Ramírez Tapias, Yuly Andrea; Lapasset Laumann, Aldana S.; Britos, Claudia Noelia; Rivero, Cintia Wanda; Trelles, Jorge Abel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Enzyme immobilization using hydrogels is a low-cost and effective system for the degradation of bulk pectin derived from orange industry residues. Polygalacturonases obtained from four different bacterial strains of Streptomyces genus were immobilized in alginate gel and assayed for pectin hydrolysis. The enzyme from Streptomyces halstedii ATCC 10897 proved to be superior and more stable within the alginate matrix. Furthermore, a new strategy to improve alginate bead stability using a mixture of calcium and strontium is reported; this technique allowed enhancing the mechanical properties by combining different amounts of these cations for ionotropic gelation. The developed biocatalyst showed maximum hydrolysis at 2 h, generating 1.54 mg/mL of reducing sugars and decreasing the viscosity of polygalacturonic acid by 98.9%. Reusability up to 29 successive reactions (58 h) demonstrated a very stable performance. The heterogeneous biocatalyst was used in the enzymatic saccharification of orange peel albedo (2.23 mg/mL) for adding value to this agro-waste by industrial exploitation.
Fil: Ramírez Tapias, Yuly Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Lapasset Laumann, Aldana S.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Britos, Claudia Noelia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Rivero, Cintia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Materia
Calcium And Strontium Mixture
Enzymatic Stability
Immobilized Biocatalyst
Reusability
Streptomyces Halstedii Atcc 10897
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41472
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41472
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrixRamírez Tapias, Yuly AndreaLapasset Laumann, Aldana S.Britos, Claudia NoeliaRivero, Cintia WandaTrelles, Jorge AbelCalcium And Strontium MixtureEnzymatic StabilityImmobilized BiocatalystReusabilityStreptomyces Halstedii Atcc 10897https://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Enzyme immobilization using hydrogels is a low-cost and effective system for the degradation of bulk pectin derived from orange industry residues. Polygalacturonases obtained from four different bacterial strains of Streptomyces genus were immobilized in alginate gel and assayed for pectin hydrolysis. The enzyme from Streptomyces halstedii ATCC 10897 proved to be superior and more stable within the alginate matrix. Furthermore, a new strategy to improve alginate bead stability using a mixture of calcium and strontium is reported; this technique allowed enhancing the mechanical properties by combining different amounts of these cations for ionotropic gelation. The developed biocatalyst showed maximum hydrolysis at 2 h, generating 1.54 mg/mL of reducing sugars and decreasing the viscosity of polygalacturonic acid by 98.9%. Reusability up to 29 successive reactions (58 h) demonstrated a very stable performance. The heterogeneous biocatalyst was used in the enzymatic saccharification of orange peel albedo (2.23 mg/mL) for adding value to this agro-waste by industrial exploitation.Fil: Ramírez Tapias, Yuly Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; ArgentinaFil: Lapasset Laumann, Aldana S.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; ArgentinaFil: Britos, Claudia Noelia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; ArgentinaFil: Rivero, Cintia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; ArgentinaFil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; ArgentinaSpringer Verlag Berlín2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41472Ramírez Tapias, Yuly Andrea; Lapasset Laumann, Aldana S.; Britos, Claudia Noelia; Rivero, Cintia Wanda; Trelles, Jorge Abel; Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix; Springer Verlag Berlín; 3 Biotech; 7; 6; 12-20172190-5738CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s13205-017-1010-4info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs13205-017-1010-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:35Zoai:ri.conicet.gov.ar:11336/41472instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:36.012CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix
title Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix
spellingShingle Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix
Ramírez Tapias, Yuly Andrea
Calcium And Strontium Mixture
Enzymatic Stability
Immobilized Biocatalyst
Reusability
Streptomyces Halstedii Atcc 10897
title_short Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix
title_full Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix
title_fullStr Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix
title_full_unstemmed Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix
title_sort Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix
dc.creator.none.fl_str_mv Ramírez Tapias, Yuly Andrea
Lapasset Laumann, Aldana S.
Britos, Claudia Noelia
Rivero, Cintia Wanda
Trelles, Jorge Abel
author Ramírez Tapias, Yuly Andrea
author_facet Ramírez Tapias, Yuly Andrea
Lapasset Laumann, Aldana S.
Britos, Claudia Noelia
Rivero, Cintia Wanda
Trelles, Jorge Abel
author_role author
author2 Lapasset Laumann, Aldana S.
Britos, Claudia Noelia
Rivero, Cintia Wanda
Trelles, Jorge Abel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Calcium And Strontium Mixture
Enzymatic Stability
Immobilized Biocatalyst
Reusability
Streptomyces Halstedii Atcc 10897
topic Calcium And Strontium Mixture
Enzymatic Stability
Immobilized Biocatalyst
Reusability
Streptomyces Halstedii Atcc 10897
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Enzyme immobilization using hydrogels is a low-cost and effective system for the degradation of bulk pectin derived from orange industry residues. Polygalacturonases obtained from four different bacterial strains of Streptomyces genus were immobilized in alginate gel and assayed for pectin hydrolysis. The enzyme from Streptomyces halstedii ATCC 10897 proved to be superior and more stable within the alginate matrix. Furthermore, a new strategy to improve alginate bead stability using a mixture of calcium and strontium is reported; this technique allowed enhancing the mechanical properties by combining different amounts of these cations for ionotropic gelation. The developed biocatalyst showed maximum hydrolysis at 2 h, generating 1.54 mg/mL of reducing sugars and decreasing the viscosity of polygalacturonic acid by 98.9%. Reusability up to 29 successive reactions (58 h) demonstrated a very stable performance. The heterogeneous biocatalyst was used in the enzymatic saccharification of orange peel albedo (2.23 mg/mL) for adding value to this agro-waste by industrial exploitation.
Fil: Ramírez Tapias, Yuly Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Lapasset Laumann, Aldana S.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Britos, Claudia Noelia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Rivero, Cintia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
description Enzyme immobilization using hydrogels is a low-cost and effective system for the degradation of bulk pectin derived from orange industry residues. Polygalacturonases obtained from four different bacterial strains of Streptomyces genus were immobilized in alginate gel and assayed for pectin hydrolysis. The enzyme from Streptomyces halstedii ATCC 10897 proved to be superior and more stable within the alginate matrix. Furthermore, a new strategy to improve alginate bead stability using a mixture of calcium and strontium is reported; this technique allowed enhancing the mechanical properties by combining different amounts of these cations for ionotropic gelation. The developed biocatalyst showed maximum hydrolysis at 2 h, generating 1.54 mg/mL of reducing sugars and decreasing the viscosity of polygalacturonic acid by 98.9%. Reusability up to 29 successive reactions (58 h) demonstrated a very stable performance. The heterogeneous biocatalyst was used in the enzymatic saccharification of orange peel albedo (2.23 mg/mL) for adding value to this agro-waste by industrial exploitation.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41472
Ramírez Tapias, Yuly Andrea; Lapasset Laumann, Aldana S.; Britos, Claudia Noelia; Rivero, Cintia Wanda; Trelles, Jorge Abel; Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix; Springer Verlag Berlín; 3 Biotech; 7; 6; 12-2017
2190-5738
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41472
identifier_str_mv Ramírez Tapias, Yuly Andrea; Lapasset Laumann, Aldana S.; Britos, Claudia Noelia; Rivero, Cintia Wanda; Trelles, Jorge Abel; Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix; Springer Verlag Berlín; 3 Biotech; 7; 6; 12-2017
2190-5738
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s13205-017-1010-4
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs13205-017-1010-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Verlag Berlín
publisher.none.fl_str_mv Springer Verlag Berlín
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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